cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus collilineatus (Cdc PLA2). The cDNA encoding Cdc PLA2 from whole venom was sequenced. The deduced amino acid sequence of this cDNA has high overall sequence identity with the group II PLA2 protein family. Cdc PLA2 has 14 cysteine residues capable of forming seven disulfide bonds that characterize this group of PLA2 enzymes. Cdc PLA2 was isolated using conventional Sephadex G75 column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC). The molecular mass was estimated using matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. We tested the neuromuscular blocking activities on chick biventer cervicis neuromuscular tissue. Phylogenetic analysis of Cdc PLA2 showed the existence of two lines of N6-PLA2, denominated F24 and S24. Apparently, the sequences of the New World’s N6-F24-PLA2 are similar to those of the agkistrodotoxin from the Asian genus Gloydius. The sequences of N6-S24-PLA2 are similar to the sequence of trimucrotoxin from the genus Protobothrops, found in the Old World.

Overview of Zika virus (ZIKV) infection in regards to the Brazilian epidemic

Zika virus (ZIKV), a mosquito-borne flavivirus, belongs to the Flaviviridae family, genus Flavivirus. ZIKV was initially isolated in 1947 from a sentinel monkey in the Zika forest, Uganda. Little clinical importance was attributed to ZIKV, once only few symptomatic cases were reported in some African and Southeast Asiatic countries. This situation changed in 2007, when a large outbreak was registered on the Yap Island, Micronesia, caused by the Asian ZIKV lineage. Between 2013 and 2014, ZIKV spread explosively and caused many outbreaks in different islands of the Southern Pacific Ocean and in 2015 autochthonous transmission was reported in Brazil. Currently, Brazil is the country with the highest number of ZIKV-positive cases in Latin America. Moreover, for the first time after the discovery of ZIKV, the Brazilian scientists are studying the possibility for the virus to cause severe congenital infection related to microcephaly and serious birth defects due to the time-spatial coincidence of the alarming increase of newborns with microcephaly and the Brazilian ZIKV epidemic. The present review summarizes recent information for ZIKV epidemiology, clinical picture, transmission, diagnosis and the consequences of this emerging virus in Brazil.

Coping with globalization: Asian versus Latin American strategies of development, 1980-2010

When compared to Latin America, Asian economies since 1980 have grown faster and have done so with relatively modest inequalities. Why? A comparison of Asia and Latin America underlines the superiority of the nationalist capitalist model of development, which has often been pursued more explicitly in Asia, over that of a dependent capitalist model, which has often been pursued in Latin America. In comparison to Latin America, the Asian model has facilitated higher and less volatile rates of economic growth and a greater political room to pursue social democratic policies. The "tap root" of these alternate pathways is relative autonomy from global constraints: states and economies in Asia have been more nationalist and autonomous than in Latin America.

Gender and human security : the challenge facing Canada's human security policy /

During the 1980's and for much of the 1990's, many countries in the Asia Pacific were renowned for their economic development and prosperity. The Asian tigers were a source of great interest for many economists and international investors. The 1997 Asian financial crisis, however, dramatically altered the growth and the performance of these economies. The crisis sent several ofAsia's best performing economies on a downward spiral from which many have yet to fully recover. The crisis exposed the financial and the political weaknesses ofmany countries in the region. Moreover, the crisis severely affected the wellbeing and the security ofmany ofthe region's citizens. This text will examine the economic crisis in greater detail and explore current debates in the study of international relations theory. More specifically, this paper will examine recent challenges posed to traditional international relations theory and address alternative approaches to this field of study. This paper will examine Critical theory and its role in shifting the referent object of security from the state to the individual. In this context, this paper will also assess Critical theory's role in enabling such issues as gender and human security to find a place on the agendas of international relations scholars and foreign policy makers. The central focus ofthis study will be the financial crisis and its impact on human security in the Southeast Asia. Furthermore, this paper will assess the recovery efforts ofthe domestic governments, international organizations and various Canadian sponsored initiatives in the context ofhuman security.

South-West Monsoon Rainfall of Kerala and Its Variability

This study focuses on the south –west monsoon rainfall over Kerala and its variability both on the spatial and temporal scales. The main objectives of the study are, interanual, long-term and decadal variabilities in MRF(monsoon rain fall),relationship between antecedent global circulation parameters, diurnal variability using data of a large number of stations in Kerala and the spatial distribution of rainfall under two large scale synoptic. Kerala gets nearly 190cm of rainfall during the south-west monsoon season 1st June to 30th September. This is more than twice the monsoon rainfall of India. A good part of kerala’s rainfall is caused by the orography of the Western Ghats Mountain ranges. The state receives 286cm of annual rainfall of which 68%is during the south-west monsoon season. The summer monsoon rainfall of Kerala shows a decreasing trend of 12.0%in 96 years. The study shows that the Intra Seasonal Oscillations(ISO) of the monsoon season has large interanual variability,some years having long period and other years having short period ISO. It is seen that Western Ghats has a strong control on the east west profile on the monsoon rainfall.

AN INVESTIGATION ON AIR-SEA INTERACTION IN THE INDIAN OCEAN DURING CONTRASTING MONSOONS

The study is undertaken with an objective to investigate the linkage between air-sea fluxes in the Indian Ocean and monsoon forcing. Since the monsoon activity is linked to fluxes, the variability of surface marine meteorological fields under the variable monsoon conditions is also studied. The very objective of the present study is to document various sea surface parameters of the Indian Ocean and to examine the anomalies found in them. Hence it is attempted to relate the anomaly to the variability of monsoon over India, highlighting the occasion of contrasting monsoon periods. The analysis of anomalies of surface meteorological fields such as SST, wind speed and direction, sea level pressure and cloud cover for contrasting monsoons are also studied. During good monsoon years, the pressure anomalies are negative indicating a fall in SLP during pre-monsoon and monsoon months. The interaction of the marine atmosphere with tropical Indian Ocean and its influence on ISMR continue to be an area of active research.

Interannual Variability of Upwelling Indices in the Southeastern Arabian Sea: A Satellite Based Study

Increase in sea surface temperature with global warming has an impact on coastal upwelling. Past two decades (1988 to 2007) of satellite observed sea surface temperatures and space borne scatterometer measured winds have provided an insight into the dynamics of coastal upwelling in the southeastern Arabian Sea, in the global warming scenario. These high resolution data products have shown inconsistent variability with a rapid rise in sea surface temperature between 1992 and 1998 and again from 2004 to 2007. The upwelling indices derived from both sea surface temperature and wind have shown that there is an increase in the intensity of upwelling during the period 1998 to 2004 than the previous decade. These indices have been modulated by the extreme climatic events like El–Nino and Indian Ocean Dipole that happened during 1991–92 and 1997–98. A considerable drop in the intensity of upwelling was observed concurrent with these events. Apart from the impact of global warming on the upwelling, the present study also provides an insight into spatial variability of upwelling along the coast. Noticeable fact is that the intensity of offshore Ekman transport off 8oN during the winter monsoon is as high as that during the usual upwelling season in summer monsoon. A drop in the meridional wind speed during the years 2005, 2006 and 2007 has resulted in extreme decrease in upwelling though the zonal wind and the total wind magnitude are a notch higher than the previous years. This decrease in upwelling strength has resulted in reduced productivity too.

Intraseasonal oscillation of total precipitable water over North Indian Ocean and its application in the diagnostic study of coastal rainfall

TRMM Microwave Imager (TMI) is reported to be a useful sensor to measure the atmospheric and oceanic parameters even in cloudy conditions. Vertically integrated specific humidity, Total Precipitable Water (TPW) retrieved from the water vapour absorption channel (22GHz.) along with 10m wind speed and rain rate derived from TMI is used to investigate the moisture variation over North Indian Ocean. Intraseasonal Oscillations (ISO) of TPW during the summer monsoon seasons 1998, 1999, and 2000 over North Indian Ocean is explored using wavelet analysis. The dominant waves in TPW during the monsoon periods and the differences in ISO over Arabian Sea and Bay of Bengal are investigated. The northward propagation of TPW anomaly and its coherence with the coastal rainfall is also studied. For the diagnostic study of heavy rainfall spells over the west coast, the intrusion of TPW over the North Arabian Sea is seen to be a useful tool.

A Study on the Transport and Distribution of Atmospheric Aerosols and its Influence on Regional Meteorological Parameters over the Indian Subcontinent

This doctoral thesis addresses the growing concern about the significant changes in the climatic and weather patterns due to the aerosol loading that have taken place in the Indo Gangetic Plain(IGP)which includes most of the Northern Indian region. The study region comprises of major industrial cities in India (New Delhi, Kanpur, Allahabad, Jamshedpur and Kolkata). Northern and central parts of India are one of the most thickly populated areas in the world and have the most intensely farmed areas. Rapid increase in population and urbanization has resulted in an abrupt increase in aerosol concentrations in recent years. The IGP has a major source of coal; therefore most of the industries including numerous thermal power plants that run on coal are located around this region. They inject copious amount of aerosols into the atmosphere. Moreover, the transport of dust aerosols from arid locations is prevalent during the dry months which increase the aerosol loading in theatmosphere. The topography of the place is also ideal for the congregation of aerosols. It is bounded by the Himalayas in the north, Thar Desert in the west, the Vindhyan range in the south and Brahmaputra ridge in the east. During the non‐monsoon months (October to May) the weather in the location is dry with very little rainfall. Surface winds are weak during most of the time in this dry season. The aerosols that reach the location by means of long distance transport and from regional sources get accumulated under these favourable conditions. The increase in aerosol concentration due to the complex combination of aerosol transport and anthropogenic factors mixed with the contribution from the natural sources alters the optical properties and the life time of clouds in the region. The associated perturbations in radiative balance have a significant impact on the meteorological parameters and this in turn determines the precipitation forming process. Therefore, any change in weather which disturbs the normal hydrological pattern is alarming in the socio‐economic point of view. Hence, the main focus of this work is to determine the variation in transport and distribution of aerosols in the region and to understand the interaction of these aerosols with meteorological parameters and cloud properties.

A Study on Aerosol Transport Dynamics over the Indian Landmass and the Adjoining Oceanic Regions Using Satellite Data

The present study brings out the influence of transport dynamics on the aerosol distribution over the Indian region at a few selected geographically distinct locations. Over the Bay of Bengal the dominant pathway of aerosol transport during the pre-monsoon period is through higher altitudes (~ 3 km); directed from the Indian main land. In contrast, the aerosol pathways over the Arabian Sea during the same period are quite complex. They are directed from geographically different environments around the ocean through different altitudes. However in general, the day-to-day variability of AOD at both these regions is significantly influenced by the features of atmospheric circulation especially, the wind convergence at higher altitudes (around 3 km). Over the Ganga Basin during the winter period, the wind convergence at lower altitudes (< I km) govems the shon term variations in AOD, while the mean AOD distribution at this location is mainly governed by the local anthropogenic sources.

Dynamics and Thermodynamics of the Arabian Sea Warm Pool

The present study examines the importance of low saline waters and resulting barrier layer in the dynamics of the ASWP using observational data.The oceanic general circulation models (OGCM) are very useful for exploring the processes responsible for the ASWP and their variability. The circulation and thermohaline structure stimulated by an OGCM changes a lot when the resolution is increased from mesoscale to macro scale. For a reasonable simulation of the ASWP, we must include the mesoscale turbulence in numerical models. Especially the SEAS is an eddy prominent region with a horizontal dimension of 100 to 500 km and vertical extent of hundred meters. These eddies may have an important role on the evolution of ASWP, which has not been explored so far.Most of the earlier studies in the SEAS showed that the heat buildup in the mixed layer during the pre-monsoon (March-May) is primarily driven by the surface heat flux through the ocean-atmosphere interface, while the 3-dimensional heat budget of the ML physical processes that are responsible for the formation of the ASWP are unknown. With this background the present thesis also examines the relative importance of mixed layer processes that lead to the formation of warm pool in the SEAS.

Contribution of size fractions of planktonic algae to primary organic productivity in the coastal waters of Cochin- south west coast of lndia

This thesis entitled “Contribution of size fractions of planktonic algae to primary organic productivity in the coastal waters of cochin,south west coast of india”. Marine ecosystems planktonic algae are the most important primary producers on wliich considerable attention is being given on account of their supreme status in the marine food chain.The study of primary production in the Indian Ocean started With DANA (I928-30),, John Murray t I933-34). Discovery ( I934) and Albatross (I947-48) expeditions which tried to evaluate productivity from nutrients and standing crop of phytoplankton .The bioproductivity of the marine environment is dependent on various primary producers. ranging in size from picoplankton to larger macro phytoplankton. The quantity and quality of various size fractions of planktonic algae at any locality depend mainly on the hydrographic conditions of the area .In the coastal waters of Cochin- south west coast of lndia. Planktonic algal community is composed mainly of the diatoms, the dinoflagellates, the blue-green algae and the silicoflagellates, the former two contributing the major flora and found distributed in the all size fractions. The maximum number of species of diatoms at station 1 and station 2 was found in the pre-monsoon season.. The size groups of planktonic algae greater than 53 um are dominated by filamentous- chain forming and colonial diatoms. The coastal waters of Cochin. planktonic algae less than 53 um in size contribute significantly to primary productivity and the biodiversity of the microflora, indicating the presence of rich fishery resources in the south west coast of india.The study of different size fractions of planktonic algae and their relative contribution to the primary organic production is a useful tool for the estimation of the quantity and quality of fisheries.A deeper investigation on the occurrence of these microalgae and proper identification of their species would be of immense help for the assessment of the specificity and magnitude of fishery resources.

Studies on the Back Water Oyster, grassostrea madra— sensis (Preston) of Cochin Harbour

A detailed study of the hydrography of the Cochin Backwaters, the habitat off crassostrea madrasensis has been carried out. Data pertaining to air temperature, water temperature, salinity, dissolved oxygen, turbidity and rainfall have been collected and presented. The temperature fluctuation was in the range of 5°C only and that of salinity between 1.1%o and 32.9%o. Fairly steady salinity has been recorded during the pre-monsoon period (February to May) and drastic declension during the monsoon period (June-September).Dissolved oxygen varied between 2.5 ml/l and 6.5 ml/l. Turbidity was highest in June (27.9 p.p.m.) and minimum (10.2 p.p.m.) in February. A detailed study on marine biofouling in the Cochin Backwaters has been made with special reference to primary film, settlement and growth of the fouling organisms such as hydroids, bryozoans, tube-dwelling polychaetes, barnacles and modiolus

Computational models for the prediction of severe thunderstorms over east indian region

Thunderstorm is one of the most spectacular weather phenomena in the atmosphere. Many parts over the Indian region experience thunderstorms at higher frequency during pre-monsoon months (March- May), when the atmosphere is highly unstable because of high temperatures prevailing at lower levels. Most dominant feature of the weather during the pre-monsoon season over the eastern Indo-Gangetic plain and northeast India is the outburst of severe local convective storms, commonly known as ‘Nor’wester’ or ‘Kalbaishakhi’. The severe thunderstorms associated with thunder, squall line, lightning and hail cause extensive losses in agriculture, damage to structure and also loss of life. The casualty due to lightning associated with thunderstorms in this region is the highest in the world. The highest numbers of aviation hazards are reported during occurrence of these thunderstorms. In India, 72% of tornadoes are associated with this thunderstorm.

Characteristics of ABL during active and weak phases of monsoon

Understanding of the Atmospheric Boundary Layer (ABL) is imperative in the arena of the monsoon field. Here, the features of the ABL are studied employing Conserved Variable Analysis (CVA) using equivalent potential temperature and humidity. In addition, virtual potential temperature and wind are used during active and weak phases of monsoon. The analysis is carried out utilising the radiosonde observations during the monsoon months for two stations situated in the west coast of India. All these parameters show considerable variations during active and weak monsoon phases in both the stations. The core speed and core height vary with these epochs. The core speed is found to be more than 38 knots in the active monsoon phase around 1.2 km over Trivandrum and around 2 km over Mangalore. But during weak monsoon phase the core wind speed is decreased and core height is elevated over both stations. The wind direction shows an additional along shore component during weak monsoon period. The Convective Boundary Layer (CBL) height shows increase during weak monsoon phase over both stations due to less cloudiness and subsequent insolation. The CBL height during the southwest monsoon is more over Mangalore and is attributed by the orographic lifting in the windward side of the Western Ghats while the influence of the Ghats is less over Trivandrum.