963 resultados para TRANSFUSÃO DE SANGUE


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The study evaluated the performance and carcass composition index of Nile tilapia (Oreochromis niloticus) fed with diets containing increasing levels of spray-dried blood meal (SDBM) and vat-dried blood meal (VDBM) and formulated based on digestible amino acids. Two hundred and fifty-two fingerlings were distributed in a completely randomized design, in a (2 x 4) + 1 factorial model, two types of blood meal with four levels of each blood meal in the diet, and a control diet (without blood meal), with four replications. The treatments consisted of soybean meal-based control diet, with 34% digestible protein (DP) and 3,200 kcal of digestible energy kg-1 (DE), plus four diets formulated with SDBM and four diets with VDBM, containing 5, 10, 15 and 20% of each meal in feed, maintaining identical DP, DE, phosphorus, calcium, lysine, methionine, threonine and tryptophan levels as those of the control diet. The results show that it is possible to use up to 15% VDBM in diets of Nile tilapia (O. niloticus) between 5 to 150 g of body weight.

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Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Apocynin, a methoxy-catechol originally extracted from the root of Picrorhiza kurroa, has been used as an inhibitor of the NADPH oxidase complex in phagocytic and nonphagocytic cells. Its mechanism of inhibition is linked to their prior activation through the action of peroxidases leading to oxidation of the dimeric product, diapocynin. In this study, dipocinina was synthesized and investigated its effect as an inhibitor of activation NADPH oxidase in neutrophils (PMN) and peripheral blood mononuclear cells (PBMC). The synthesis of diapocinina was performed by oxidation of apocinina by potassium persulphate in the midst of water for 5 minutes at room temperature. The precipitate was filtered and washed with water and methanol. Diapocinina was characterized by mass spectrometry. PMN and PBMC were obtained from peripheral blood of healthy donors and purified for gelatin sedimentation, or centrifugation with Histopaque ®, the red cells were lysed with ice water or ammonium chloride. Diapocinina or apocinina were incubated with opsonized zymosan, activation of PMNs and release of superoxide anion, these monitored by chemiluminescent assay dependent lucigenina. We found that diapocinina inhibitor was no better than the apocinina in PMN. However, diapocinina was more efficient than apocinina as an inhibitor of NADPH oxidase in PBMC. In conclusion, whereas PBMC are relatively poor compared with peroxidases PMN, our results are consistent with the need for oxidation apocinina for its effect as an inhibitor of NADPH oxidase

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Os estudos hematológicos das diferentes espécies de peixes são de interesse ecológico e fisiológico, auxiliando na compreensão da relação entre as características sanguíneas, a filogenia, a atividade física, o hábitat e a adaptabilidade dos peixes ao ambiente. No experimento realizado foram testados os efeitos de águas contaminadas em parâmetros hematológicos de peixes da espécie Prochilodus lineatus, em períodos de coleta de 7 e 20 dias, nos quais o sangue foi coletado com seringas heparinizadas, foram montadas lâminas de esfregaço , as quais foram coradas pelo corante de Leishman. Estas lâminas foram analisadas e fotografadas com o auxílio de um microscópio óptico Leica, no qual foram feitas contagens totais de células brancas e contagens diferenciais de trombócitos e leucócitos, para a análise estatística. O grupo exposto ao Lago Azul apresentou uma elevação no número de leucócitos e no total de células brancas, evidenciando que os contaminantes químicos do ambiente estavam atuando de forma semelhante a um antígeno no corpo do animal fazendo com que suas células de defesa se proliferassem. Quanto ao grupo exposto ao detergente, observou-se que após os vinte dias de experimento ocorreu uma diminuição no número de trombócitos. Tais resultados evidenciam que a variação no número de leucócitos apresentou-se como um indicador de poluição ambiental e que os detergentes biodegradáveis podem em certo tempo de exposição ocasionar um déficit em funções vitais de peixes como à coagulação e a prevenção contra infecções, eventos ligados diretamente com os trombócitos. A maioria dos vertebrados aquáticos possui brânquias, estruturas especializadas nas trocas gasosas e responsáveis por grande parte das trocas iônicas. Este órgão acaba por absorver grande parte das substâncias presentes na água, que ao caírem na...(Resumo completo, clicar acesso eletrônico abaixo)

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Blood transfusion is a very important therapeutic and profilatic procedure. However, the most part of the transfusions in animals in Brazil are done with total blood. The transfusion therapy made in an appropriate way, with hemotherapic products, can save lives and warranties a better health to patients. In all transfusion procedures, the cross match test must be done because there are lots of different blood group types, based on erythrocytes antigen. A slow infusion rate and intensive patient monitoration help to prevent transfusion reactions, mainly hemolytic reactions

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Equine neonatal isoerythrolysis is a neonatal foals’ illness. Results from the incompatibility of blood type between the foal and the mare and mediated by maternal antibody absorbed by the colostrum against foal’s red blood cells. Characterized by a type ll hypersensitivity reaction, where the exhibition of the organism to a strange antigen, that it takes the sensitization of the lymphocytes B that after the removal of the antigens by the reticule-endothelial system the production of immunoglobulin is decreased, with the formation of cellular immunological will cause the occurrence of the illness in foal of sensitized mares. The most important clinical signs are severe anemia and jaundice, and this illness should be differentiated of other as: hemolysis induced by bacterial toxins, diseases of the hepatobiliary system, disseminated intravascular coagulation and incompatibility in blood transfusions. Like the sensitization happens during the previous incompatible foal’s birth, most cases occur in foals of multiparous mares. However during the first pregnancy the mare can generate a foal with neonatal 7 isoerythrolysis if she have developed placental anomaly in the beginning of the pregnancy which blood cells in her circulation

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Objective - At evaluating the IL-10 production in maternal blood and placenta and correlate them with perinatal outcomes in pregnancies complicated by hyperglycemia, or with risk to developing it, differentiated by the glycemic mean (GM < or ≥ 100mg/dL). Method- 186 pregnant women were distributed into groups GM < 100 mg/dL and GM ≥ 100 mg/dL. We evaluated the GM, HbA1c levels, maternal and placental IL-10 and TNF-α and the correlation between placental cytokines and perinatal outcomes. Results - In maternal blood, the lower concentrations of IL-10 (1.01 ± 0.87 vs. 3.08 ± 5.57 pg / mL, p = 0.0019) were observed in GM ≥ 100 mg / dL group. Placental IL-10 was directly correlated with hemoglobin levels (r = 0.63, p = 0.02) and insulin (r = 0.78, p = 0.01) from umbilical cord and with Apgar scores 1 (r = 0.53, p = 0.0095) and Apgar 5 (r = 0.69, p = 0.0003). Conclusion - GM ≥ 100mg/dL was associated with decreased of maternal IL-10. Placental IL-10 was similar in both groups and correlated directly with hemoglobin and insulin and with Apgar scores of 1st. and 5th. minutes

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Os valores encontrados para a viscosidade sanguínea tendem a aparecer com diferenças significativas, de acordo com o sexo, avançar da idade e uso de substâncias. Pelo fato de o sangue ser um fluido não newtoniano, não podemos expressar sua viscosidade em termos absolutos sem que sejam levadas em conta as condições em que a mensuração é feita. Estudos anteriores destacaram a importância esta medida, considerando o efeito da idade, o uso de substâncias, bem como sua relação com Fluxo Sanguíneo Regional Cerebral, o que pode indicar correlação com declínios cognitivos. Entretanto, a prática médica carece ainda de um método simples e clinicamente prático para a verificação desta medida, além de valores de referência. Como passo inicial no sentido de padronizar os procedimentos e condições do processo de medição, avaliamos a quantidade mínima de amostra necessária para a medição da viscosidade sanguínea, utilizando este equipamento. Um total de 20 amostras de sangue foram coletadas de indivíduos saudáveis, entre 18 e 60 anos, em tubos com EDTA, e cada amostra dividida em 9 sub-amostras (de 600μl, 550μl, 500μl, 450μl, 400μl, 350μl, 300μl, 250μl e 200μl), totalizando, portanto, um total de aproximadamente 180 medições. A quantidade de 200 μl apresentou diferenças significativas com relação à primeira medida obtida, quando comparada com todas as demais quantidades (p<0,001), o que indica que com o uso desta quantidade, não é possível obter resultados confiáveis, pois existe alteração nos valores de VS obtidos relacionada à quantidade de material utilizado. Considerando a menor quantidade, bem como, que estivesse dentro da faixa de variação aceitável de 0,2 mPa.s, e a qual não apresentasse diferença significativa quando comparadas às demais (p>0,05), foi verificada a quantidade de 250 μl como a mais eficiente... (Resumo completo, clicar acesso eletrônico abaixo)

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Our previous studies have shown that low concentrations (noncytotoxics) of antineoplastic agents modulate positively the dendritic cells, favoring their in vitro maturation and improving their antigen presenting function. The effects on colorectal cancer cells (HCT-116) were also investigated and we have observed an increased immunogenicity and susceptibility to cytotoxic T cells. Thereby, this study aimed to investigate the effect of 5-fluorouracil (5-FU) an azacitidine (AZA), in minimum effective and noncytotoxic concentrations on lymphocytes of healthy donors. In this study we have analyzed the cytotoxic effect of drugs at these concentrations as well as the proliferative ability of lymphocytes. In vitro production of IL-10 and IFN-γ has been also evaluated. We have observed that low concentrations of those chemotherapeutic agents are not cytotoxic for lymphocytes. However, the minimum effective concentrations (5-FU: 0,410±0,088 e AZA: 0,757±0,233; p<0, 05) have reduced the cell number. Proliferative activity of allogeneic lymphocytes in a mixed reaction (MLR) was not affected by the treatment. The cytokine production was not affected by the treatments, either. In conclusion, low concentrations of 5-FU and AZA has no deleterious effects on human peripheral blood lymphocytes and seems to be safe for combinatory administration with DC vaccines

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Pós-graduação em Medicina Veterinária - FCAV

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Blood is one of the most important and powerful elements in magical and religious symbology. As it is an essential substance for animal survival, it does not sound strange that through the ages blood has been given many significant symbolic values (both positive and negative). It can provide life, protection and prosperity in the same proportion that it can cause calamities, destruction and even death. In Opus agriculturae, a farming treatise written by Palladius (V AD), more specifically in Book I, Chapter 35, the author presents prescriptions to protect farms against scourges and climatic phenomena. He mentions some procedures in which blood is a fundamental component in the success of the prescriptions. The aim of the present article is to identify any magic power associated with these practices, their specific symbolic value, especially when related to menstrual blood and to other feminine elements with some magical or religious value. An evaluation is also made of the extent of these symbologies in the context of other practices and situations linked not only to agricultural magic, but to religion and to the ritual practices of other communities, whether in ancient times or not.