Influ��ncia do desponte de pan��culas de sorgo sacarino sobre a qualidade da mat��ria-prima e produ����o de bioetanol

P��s-gradua����o em Microbiologia Agropecu��ria - FCAV

Produ����o de bioetanol a partir da fermenta����o de caldo de sorgo sacarino e cana-de-a����car

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Ac��mulo de nutrientes e tempo de decomposi����o da palhada de esp��cies forrageiras em fun����o de ��pocas de semeadura

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Ac��mulo de mat��ria seca e macronutrientes por cultivares de sorgo sacarino

Nutrient accumulation curves help us understand the nutritional demands of a crop. The aim of this study was to evaluate the growth and accumulation of macronutrients by the sweet sorghum cultivars CVSW80007, CVSW80147, CVSW82028, CVSW82158. The experiment was conducted in a renewal sugarcane ratoon area at Uchoa county, SP, being deployed in January 2012. The plants were collected in 15 days intervals from the 10th after emergence (DAE). The greatest content of dry matter, N, P, Mg and S, was due to by the CVSW82028 cultivar, while the CVSW82158 extract the greatest amount of K and the CVSW80147 of Ca. The stalks represented the highs percentage of dry matter and nutrient content, except nitrogen and phosphorus, in which grains corresponded to the higher percentage of total absorption. The CVSW80007, CVSW82028 and CVSW82158 cultivars showed the following decreasing nutrient extraction N>K>Ca>Mg>P>S and the CVSW80147, K>N>Ca>Mg>P>S. Considering only the stalks harvest, the decreasing nutrient exportation is the same for all cultivars: K>N>Ca>Mg>S>P. Whereas the decreasing nutrient exportation sequence considering stalks and grain harvest is the following for the CVSW80007, CVSW80147 and CVSW82158 cultivars: K>N>Ca>Mg>P>S and K>N>Ca>Mg>P>S for the CVSW82028.

Effect of cropping systems in no-till farming on the quality of a Brazilian Oxisol

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Couve consorciada com sorgo e feij��o-guandu na ocorr��ncia de pulg��es e insetos predadores

P��s-gradua����o em Agronomia (Entomologia Agr��cola) - FCAV

La podredumbre del cuello del sorgo causada por Fusarium verticilloides

El sorgo anual ha sido clasificado por de Wet (1978) como Sorghum bicolor (L.) Moench (2n=20) subespecie bicolor, con 5 razas b��sicas: bicolor, guinea, caudatum, kafir y durra, las que incluyen los distintos tipos de sorgo existentes en el mercado como los graniferos, los forrajeros tipo sud��n, dulces y escoberos (Giorda y Cordes, 2005).

Perspectivas del cultivo de sorgo azucarero para bio-etanol en Espa��a en base a resultados varietales en ensayos multilocales

La especie Sorghum bicolor (L.) Moench., perteneciente a la familia de las gram��neas (Poaceae), es una planta de origen tropical, sensible al fotoperiodo y a la temperatura, que muestra una alta eficiencia fotosint��tica en condiciones adecuadas de cultivo.

Isolation and Characterization of Glutathione S-Transferase Isozymes from Sorghum1

Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2,-trifluoro-4���-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelectric point of 4.9. GST A1/A1 exhibited high activity with 1-chloro-2, 4,dinitrobenzene (CDNB) but low activity with the chloroacetanilide herbicide metolachlor. For GST A1/A1, the random, rapid-equilibrium bireactant kinetic model provided a good description of the kinetic data for the substrates CDNB and glutathione (GSH). GST B1/B2 was a heterodimer with subunit molecular masses of 26 kD (designated the B1 subunit) and 28 kD (designated the B2 subunit) and a native isoelectric point of 4.8. GST B1/B2 exhibited low activity with CDNB and high activity with metolachlor as the substrate. The kinetics of GST B1/B2 activity with GSH and metolachlor fit a model describing a multisite enzyme having two binding sites with different affinities for these substrates. Both GST A1/A1 and GST B1/B2 exhibited GSH-conjugating activity with ethacrynic acid and GSH peroxidase activity with cumene hydroperoxide, 9-hydroperoxy-trans-10,cis-12-octadecadienoic acid and 13-hydroperoxy-cis-9,trans-11-octadecadienoic acid. Both GST A1/A1 and GST B1/B2 are glycoproteins, as indicated by their binding of concanavalin A. Polyclonal antibodies raised against GST A1/A1 exhibited cross-reactivity with the B1 subunit of GST B1/B2. Comparisons of the N-terminal amino acid sequences of the GST A1, B1, and B2 subunits with other type I ��-GSTs indicated a high degree of homology with the maize GST I subunit and a sugarcane GST.

Structure and Expression of a Dhurrinase (��-Glucosidase) from Sorghum1

Sorghum (Sorghum bicolor L. Moench) has two isozymes of the cyanogenic ��-glucosidase dhurrinase: dhurrinase-1 (Dhr1) and dhurrinase-2 (Dhr2). A nearly full-length cDNA encoding dhurrinase was isolated from 4-d-old etiolated seedlings and sequenced. The cDNA has a 1695-nucleotide-long open reading frame, which codes for a 565-amino acid-long precursor and a 514-amino acid-long mature protein, respectively. Deduced amino acid sequence of the sorghum Dhr showed 70% identity with two maize (Zea mays) ��-glucosidase isozymes. Southern-blot data suggested that ��-glu-cosidase is encoded by a small multigene family in sorghum. Northern-blot data indicated that the mRNA corresponding to the cloned Dhr cDNA is present at high levels in the node and upper half of the mesocotyl in etiolated seedlings but at low levels in the root���only in the zone of elongation and the tip region. Light-grown seedling parts had lower levels of Dhr mRNA than those of etiolated seedlings. Immunoblot analysis performed using maize-anti-��-glucosidase sera detected two distinct dhurrinases (57 and 62 kD) in sorghum. The distribution of Dhr activity in different plant parts supports the mRNA and immunoreactive protein data, suggesting that the cloned cDNA corresponds to the Dhr1 (57 kD) isozyme and that the dhr1 gene shows organ-specific expression.

Photoperiod Control of Gibberellin Levels and Flowering in Sorghum1

Regulation of rhythmic peaks in levels of endogenous gibberellins (GAs) by photoperiod was studied in the short-day monocot sorghum (Sorghum bicolor [L.] Moench). Comparisons were made between three maturity (Ma) genotypes: 58M (Ma1Ma1, Ma2Ma2, phyB-1phyB-1, and Ma4Ma4 [a phytochrome B null mutant]); 90M (Ma1Ma1, Ma2Ma2, phyB-2phyB-2, and Ma4Ma4); and 100M (Ma1Ma1, Ma2Ma2, PHYBPHYB, and Ma4Ma4). Plants were grown for 14 d under 10-, 14-, 16-, 18-, and 20-h photoperiods, and GA levels were assayed by gas chromatography-mass spectrometry every 3 h for 24 h. Under inductive 10-h photoperiods, the peak of GA20 and GA1 levels in 90M and 100M was shifted from midday, observed earlier with 12-h photoperiods, to an early morning peak, and flowering was hastened. In addition, the early morning peaks in levels of GA20 and GA1 in 58M under conditions allowing early flowering (10-, 12-, and 14-h photoperiods) were shifted to midday by noninductive (18- and 20-h) photoperiods, and flowering was delayed. These results are consistent with the possibility that the diurnal rhythm of GA levels plays a role in floral initiation and may be one way by which the absence of phytochrome B causes early flowering in 58M under most photoperiods.

Phytochrome B and the Regulation of Circadian Ethylene Production in Sorghum1

The sorghum (Sorghum bicolor L. Moench) cultivar 58M, which contains the null mutant phytochrome B gene, shows reduced photoperiodic sensitivity and exhibits a shade-avoidance phenotype. Ethylene production by seedlings of wild-type and phytochrome B mutant cultivars was monitored every 3 h, and both cultivars were found to produce ethylene in a circadian rhythm, with peak production occurring during the day. The phytochrome B mutant produces rhythmic peaks of ethylene with approximately 10 times the amplitude of the wild-type counterpart with the same period and diurnal timing. The source of the mutant's additional ethylene is the shoot. The diurnal rhythm can be produced with either light or temperature cycles; however, both light and temperature cycles are required for circadian entrainment. The temperature signal overrides the light signal in the production of diurnal rhythms, because seedlings grown under thermoperiods reversed with the photoperiod produced ethylene peaks during the warm nights. To examine the effect of extreme shading on ethylene production, seedlings were grown under dim, far-red-enriched light. This treatment duplicated the phytochrome B mutant's shade-avoidance phenotype in the wild type and caused the wild type to produce ethylene peaks similar to those observed in the mutant. The results confirm that phytochrome B is not required for proper function of circadian timing, but it may be involved in modulating physiological rhythms driven by the biological clock oscillator.

Desempenho agron��mico, bromatol��gico e estabilidade fenot��pica de sorgo silageiro em Uberl��ndia - MG

Sorghum (Sorghum bicolor (L.) Moench) is a good alternative to be used as silage, especially in places with water scarcity and high temperatures, due to their morphological and physiological characteristics. The appropriate management, as the ideal seeding time, interferes both productivity and the quality of silage. The work was conducted with the objective of evaluating the agronomic and bromatological performance of varieties and hybrids of silage sorghum and their phenotypic stability in two seasons, season and off-season, in the city of Uberl��ndia, Minas Gerais. The experiments were performed at Capim Branco Experimental Farm of Federal University of Uberl��ndia (UFU), located in the referred city. There were two sowing dates in the same experimental area, off-season (March to June 2014) and season (November 2014 to March 2015), and the varieties and hybrids were evaluated in both situations. The design was a randomized block with 25 treatments (hybrids and varieties of sorghum) and three replications. Agronomical and bromatological data were subjected to an analysis of variance; averages were grouped by Scott-Knott test at 5% of probability, through Genes computer program; and to estimate the stability, it was opted for Annicchiarico method. The flowering of cultivars, dry matter productivity, plant height, Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF) and Crude Protein (CP) are affected by the environment and the variety. Regarding productivity and quality of the fiber, SF11 variety was superior, independent of the rated environment. In relation to the performance stability of dry matter, the varieties SF15, SF11, SF25, PROG 134 IPA, 1141572, 1141570 and 1141562 were highlighted. For the stability of the quality of fibers (FDA and FDN), the variety 1141562 stood out. The environment reduces the expression of characters ���days of flowering���, ���plant height��� and ���productivity of dry matter of hybrids���. From the 25 hybrids analyzed for productivity and stability of dry matter performance, seven were highlighted, regardless of the rated environment: Volumax commercial hybrid and experiments 12F39006, 12F39007, 12F37014, 12F39014, 12F38009 and 12F02006.

Efeito da embalagem, periodo e local de armazenamento na qualidade de sementes do sorgo.

Sementes de sorgo (Sorghum bicolor (L.) Moench) cv. EA-955 foram condicionadas em latas de metal vedadas, sacos de pano e de polipropileno e armazenadas sob condicoes naturais, em quatro localidades do Estado do Ceara: Fortaleza, Quixada, Morada Nova e Ubajara, com o objetivo de determinar a influencia da embalagem do local e do periodo de armazenamento sobre a qua qualidade fisiologica. Mensalmente, foi determinado o teor de umidade das smentes, e a cada dois meses a qualidade das sementes era avaliada por meio de testes de germinacao, vigor (comprimento da raiz) e peso seco de plantulas. Utilizou-se um delineamento no esquema fatorial, no qual os fatores eram cinco periodos de armazenamento (0, 2, 4, 6 e 8 meses) e tres tipos de embalagem (lata, pano, polipropileno), com quatro repeticoes. Durante o periodo de armazenamento, observou-se ser a lata de metal a embalagem que melhor preserva a qualidade das sementes. No que se refere aos locais, constatou-se que as smentes armazenadas um Ubajara mostraram melhor qualidade do que as sementes estocadas nas outras localidades.

Cover Crops and Nitrogen Fertilization Effects on Nitrogen Soil Fractions under Corn Cultivation in a No-Tillage System.

ABSTRACT: The use of cover crops has recently increased and represents an essential practice for the sustainability of no-tillage systems in the Cerrado region. However, there is little information on the effects of nitrogen fertilization and cover crop use on nitrogen soil fractions. This study assessed changes in the N forms in soil cropped to cover crops prior to corn growing. The experiment consisted of a randomized complete block design arranged in split-plots with three replications. Cover crops were tested in the plots, and the N topdressing fertilization was assessed in the subplots. The following cover species were planted in succession to corn for eight years: Urochloa ruziziensis, Canavalia brasiliensis M. ex Benth, Cajanus cajan (L.) Millsp, and Sorghum bicolor (L.) Moench. After corn harvesting, the soil was sampled at depths of 0.00-0.10 and 0.10-0.20 m. The cover crops showed different effects at different soil depths. The soil cultivated with U. ruziziensis showed higher contents of total-N and particulate-N than the soil cultivated with C. cajan. Particulate-N was the most sensitive to changes in the soil management among the fractions of N assessed. The soil under N topdressing showed a lower content of available-N in the 0.10-0.20 m layer, which may be caused by the season in which the sampling was conducted or the greater uptake of the available-N by corn.