Qualidade de mudas de eucalipto produzidas sob diferentes lâminas de irrigação e dois tipos de substrato

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cultivo orgânico sequencial de hortaliças com dois sistemas de irrigação e duas coberturas de solo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Controle fuzzy espacialmente diferenciado para um sistema de irrigação

Traditional irrigation projects do not locally determine the water availability in the soil. Then, irregular irrigation cycles may occur: some with insufficient amount that leads to water deficit, other with excessive watering that causes lack of oxygen in plants. Due to the nonlinear nature of this problem and the multivariable context of irrigation processes, fuzzy logic is suggested to replace commercial ON-OFF irrigation system with predefined timing. Other limitation of commercial solutions is that irrigation processes either consider the different watering needs throughout plant growth cycles or the climate changes. In order to fulfill location based agricultural needs, it is indicated to monitor environmental data using wireless sensors connected to an intelligent control system. This is more evident in applications as precision agriculture. This work presents the theoretical and experimental development of a fuzzy system to implement a spatially differentiated control of an irrigation system, based on soil moisture measurement with wireless sensor nodes. The control system architecture is modular: a fuzzy supervisor determines the soil moisture set point of each sensor node area (according to the soil-plant set) and another fuzzy system, embedded in the sensor node, does the local control and actuates in the irrigation system. The fuzzy control system was simulated with SIMULINK® programming tool and was experimentally built embedded in mobile device SunSPOTTM operating in ZigBee. Controller models were designed and evaluated in different combinations of input variables and inference rules base

Produção da figueira submetida a diferentes épocas de poda e irrigação

O trabalho teve como objetivo avaliar diferentes épocas de poda, correspondentes aos meses de julho, agosto, setembro e outubro dos anos de 2004/05 e 2005/06, com e sem o uso de irrigação, em figueira 'Roxo de Valinhos', no município de Botucatu-SP. Para atingir tal objetivo, adotou-se o delineamento experimental em parcelas subdivididas, com 5 repetições em blocos, onde as parcelas corresponderam aos tratamentos com e sem irrigação, e as subparcelas foram constituídas pelas podas realizadas nos quatro meses do ano. Foram avaliadas características de produção e alguns atributos de qualidade, que serviram para indicar a necessidade do uso de irrigação. Os resultados obtidos permitiram concluir que, na avaliação do desdobramento da interação entre épocas de poda e irrigação complementar, o mês de agosto, com o uso de irrigação complementar, foi o mais favorável para a realização da poda da figueira, proporcionando as maiores produções médias verificadas no ensaio, tanto no ciclo agrícola de 2004/05 (3.513,8 g planta-1), quanto em 2005/06 (4.110,7 g planta-1). em condições não-irrigadas, não houve diferença estatística entre os meses de julho, agosto e setembro de 2004/05 e entre julho e agosto de 2005/06.

Acúmulo de nitrogênio, fósforo e potássio pelo algodoeiro sob irrigação cultivado em sistemas convencional e adensado

O adensamento da cultura do algodão aumenta a competição entre plantas por recursos, como luz, nutrientes e água; logo, o período de florescimento é reduzido e, consequentemente, a marcha de acúmulo de matéria seca é alterada. O objetivo deste trabalho foi determinar a marcha de absorção de N, P e K pelo algodoeiro, identificando a época de maior absorção e a quantidade absorvida e exportada em espaçamentos adensado (48 cm - 20,58 plantas m-2), intermediário (75 cm - 13,30 plantas m-2) e convencional (96 cm - 10,39 plantas m-2). Foram amostradas três plantas úteis por parcela aos 46, 69, 99, 139, 148 e 166 dias após a emergência (DAE), em que foram determinados o acúmulo de matéria seca, N, P e K. O acúmulo de matéria seca nas plantas cultivadas no espaçamento de 48 cm foi maior no início do desenvolvimento, igualando-se às demais ao final do ciclo, o que resultou em maior acúmulo de N entre os 69 e 99 DAE, no menor espaçamento. O adensamento antecipa o pico de absorção de nutrientes, sugerindo a antecipação das adubações de cobertura com N e K no algodoeiro cultivado nesse sistema. Para condições de fertilidade média/alta, a dose de nutrientes a ser aplicada não precisaria ser alterada em função do aumento da densidade de plantas, pois não há variação nas quantidades de N, P e K exportados por kg de algodão produzido, em fibra ou em caroço.

Irrigação e certificação da cafeicultura na Região Centro-Oeste de São Paulo

An unsatisfactory profitability has led to the gradual decline of the coffee plantations in São Paulo. Among the alternatives that arise for solving this problem, two stand out: the irrigation of crops and certification of properties. Even in areas considered suitable for cultivation of arabica coffee (Coffea arabica L.), the use of irrigation can promote an increase in grain yield. In addition, the product certification promotes their differentiation in the market and can improve the economic results of the activity. This study aimed to evaluate the development and productivity of coffee crops subjected to irrigation or not, comparing economic data in the marketing of coffee certified or not, under the conditions of the mid-west region of São Paulo state. The field experimental design was in randomized blocks with two treatments (with and without drip irrigation) and ten repetitions. The costs of production and profitability by selling the final product were calculated. The irrigation increased the development and productivity of coffee. The return on investment took place after the third harvest. Irrigation combined with the sale of certified coffee provided financial balance 1,192% higher than the coffee is not irrigated and non-certified.

Épocas de poda e uso da irrigação em figueira 'roxo de valinhos' na região de Botucatu, SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Teores nutricionais em folhas e frutos de figueira, submetida a épocas de poda e irrigação

The present study aimed to evaluating the nutritional content in leaves and fruits of the fig tree 'Roxo de Valinhos', pruned at different periods corresponding to the months of July, August, September and October in the years of 2004 and 2005, with and without the use of irrigation, in the county of Botucatu, São Paulo State, Brazil. To achieve this objective, the adopted experimental design was in blocks with subdivided plots and 5 replications, in which plots corresponded to treatments with and without irrigation and subplots included prunings done in the above-mentioned four months. The levels of N, P, K, Ca, Mg, S, B, Cu, Mn and Zn in leaves and fruits were evaluated in the two crop cycles. The results indicated no significant differences among macro and micronutrient levels in the leaves subjected to treatments with and without irrigation in the cycle 2004/05, except for cupper which showed higher level with the treatment including irrigation (6 mg kg(-1)). In the fruits, there was no difference, except for Zn, which also showed the highest levels (28 mg kg(-1)) with irrigation. In the crop cycle 2005/06, there were differences for N (40 g kg(-1)) and K (20 g kg(-1)) in the leaves, where the highest levels were observed with the treatment including irrigation. In the fruits, N had significant difference and its highest level was observed without irrigation (21 g kg(-1)). In relation to the pruning periods, significant differences were observed for Ca, Fe and Zn content in the leaves and Ca, K, Mg, S and Zn content in the fruits in the crop cycle 2004/05. In the cycle 2005/06, there were not differences among the levels of the evaluated nutrients in the leaves, and in the fruits there was difference for N, Ca and Cu.

Indução do florescimento e crescimento de tangerineira 'Poncã' (Citrus reticulata Blanco) em função da irrigação e da aplicação de paclobutrazol

O experimento foi conduzido na Faculdade de Ciências Agronômicas - UNESP/Câmpus de Botucatu (SP), com o objetivo de avaliar o florescimento fora de época e o crescimento vegetativo da tangerineira 'Poncã'. Nesse contexto, adotou-se o delineamento estatístico em blocos casualizados, em parcelas subdivididas, com duas repetições, na instalação do ensaio. Os dois tratamentos de -0,03 e -0,05 MPa, como potenciais mínimos da água no solo, constituíram as parcelas e as quatro doses de paclobutrazol: 0; 4; 8 e 12 g por planta, nas subparcelas. No segundo ano de pesquisa, foram mantidas as mesmas parcelas e foram aplicadas nas plantas das subparcelas as doses de 0; 500; 1000 e 2000 mg l-1 de paclobutrazol, via foliar. Cada parcela foi constituída de 16 plantas, sendo oito destinadas à avaliação. Utilizou-se, em ambas as combinações, de um tratamento sem irrigação (-0,07 MPa) e sem aplicação de paclobutrazol, como testemunha. Foram avaliados parâmetros, como a altura, diâmetro médio, volume e área de projeção da copa e condutância estomática para caracterizar a resposta das plantas aos tratamentos empregados. Concluiu-se que a aplicação do paclobutrazol e a variação do potencial da água no solo não proporcionaram a indução do florescimento fora de época das tangerineiras, e que os níveis de paclobutrazol influenciaram no desenvolvimento das plantas.

Avaliação do desenvolvimento da região de Cruzeta (RN), através do uso intensivo de água para irrigação

This work aims for the evaluation of Cruzeta Irrigated Perimeter, RN, which consists in the efficient use of water for agricultural production. The goal is looking for the available quantity of water for supplying required demands for adequate and economically viable cultures for the region. It is supposed that regional community water is supplied by pipelines from sources located outside of the region. From this study it is recommended the implantation of adequate installments for culture management in accordance with the availability of water resources and others conditionings. It must be considered the intensity of rainy and drought seasons in order to adjust the cultivated area and equipments to be operated, and also, the use of operating models and simulations in order to establish alert levels and eventually, reduction of irrigated area. Based on obtained data it is proposed the cultivation of different types of non-permanent cultures so that temporary cultures would be extensively produced in periods of abundant reservoir storage water permitting the transformation of storage water in storage culture products and few or no production in severe drought periods. This is the basic premise for sustainable agricultural development for Brazilian semiarid region

Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)

Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.

Encoding mechanisms based on fast oscillations in the retina of the cat and their dependencies on anesthesia

Processing in the visual system starts in the retina. Its complex network of cells with different properties enables for parallel encoding and transmission of visual information to the lateral geniculate nucleus (LGN) and to the cortex. In the retina, it has been shown that responses are often accompanied by fast synchronous oscillations (30 - 90 Hz) in a stimulus-dependent manner. Studies in the frog, rabbit, cat and monkey, have shown strong oscillatory responses to large stimuli which probably encode global stimulus properties, such as size and continuity (Neuenschwander and Singer, 1996; Ishikane et al., 2005). Moreover, simultaneous recordings from different levels in the visual system have demonstrated that the oscillatory patterning of retinal ganglion cell responses are transmitted to the cortex via the LGN (Castelo-Branco et al., 1998). Overall these results suggest that feedforward synchronous oscillations contribute to visual encoding. In the present study on the LGN of the anesthetized cat, we further investigate the role of retinal oscillations in visual processing by applying complex stimuli, such as natural visual scenes, light spots of varying size and contrast, and flickering checkerboards. This is a necessary step for understanding encoding mechanisms in more naturalistic conditions, as currently most data on retinal oscillations have been limited to simple, flashed and stationary stimuli. Correlation analysis of spiking responses confirmed previous results showing that oscillatory responses in the retina (observed here from the LGN responses) largely depend on the size and stationarity of the stimulus. For natural scenes (gray-level and binary movies) oscillations appeared only for brief moments probably when receptive fields were dominated by large continuous, flat-contrast surfaces. Moreover, oscillatory responses to a circle stimulus could be broken with an annular mask indicating that synchronization arises from relatively local interactions among populations of activated cells in the retina. A surprising finding in this study was that retinal oscillations are highly dependent on halothane anesthesia levels. In the absence of halothane, oscillatory activity vanished independent of the characteristics of the stimuli. The same results were obtained for isoflurane, which has similar pharmacological properties. These new and unexpected findings question whether feedfoward oscillations in the early visual system are simply due to an imbalance between excitation and inhibition in the retinal networks generated by the halogenated anesthetics. Further studies in awake behaving animals are necessary to extend these conclusions

Avaliação microscópica dos fragmentos ósseos obtidos por diferentes métodos de osteotomia e de irrigação em aloenxertos irradiados e congelados de coelho

Oral and facial bone defects can undertake appearance, psychosocial well-being and stomathognatic function of its patients. Over the yerars several strategies for bone defect regeneration have arised to treat these pathologies, among them the use of frozen and irradiated bone allograft. Manipulation of bone grafts it s not determined yet, and several osteotomy alternatives can be observed. The present work evaluated with a microscope the bone fragments obtained from different osteotomy methods and irrigation on rings and blocks allografts irradiated and frozen at 80° negative in a rabbit model. The study is experimental in vitro and it sample was an adult male New Zealand rabbit. The animal was sacrificed to obtain long bones, that were submitted to freezing at 80º negative and irradiated with Cobalt- 60. Then the long bones were sectioned into 24 bone pieces, divided into 4 groups: G1 (n=06) osteotomy was performed with bur No. 6 forming rings with 5 mm thickness with high-speed handpiece with manual irrigation; G2 (n=06) osteotomy was performed with bur No. 6 forming rings with 5 mm thick with surgical motor with a manual irrigation rotation 1500 rpm; GA (n=06), osteotomy with trephine using manual irrigation with saline; and GB (n=06), osteotomy with trephine using saline from peristaltic pumps of surgical motor. Five bone pieces of each group were prepared for analysis on light microscopy (LM) and one on electronic scan electronic microscopy (SEM). On the SEM analysis edges surface, presence of microcracks and Smear Layer were evaluated. Analyzing osteotomy technics on SEM was observed: increased presence of microcracks cutting with high speed; increased presence of areas covered by Smear Layer when cutting with motor implant. The irrigation analysis with SEM was observed: that the presence of microcracks does not depend on the type of irrigation; on manual irrigation, there was greater discrepancy between the cutting lines. The descriptive analysis of the osteotomy and irrigation process on LM showed: histological analysis showing the bony margins with clear tissue changed layer, composed of blackened tissue of charred appearance near to the cortical bone; on the edges of the bony part, bone fragments that were displaced during the bone cut and bone irregularities were observed. After analysis of results we can conclude: that there was greater regularity of the bone cut using high-speed handpiece than using motor implant; the cut with trephine using saline irrigated from peristaltic pumps of surgical motor showed greater homogeneity when compared with manual irrigation; charred tissue was found in all obtained bone samples, whit no significant statistically difference on the proportion of carbonization of the two analysed technics

Aspectos anatômicos do olho e neuroquímicos da retina do mocó (Kerodon rupestris)

The visual system is an important link between the animal and the environment, com profound influences on the habits and lifestyle in various habitats. Adaptive mechanismsto the temporal niche are present in the visual system of many vertebrates, involving changins in ocular dimensios and design, retinal cell distribution and organization of neurochemical circuits related to the retinal resolution or sensitivity. The sensory system of the eye is represented by the retina, whose organization is responsible by receipty, initial analysis, and transmission of the information to the brain. The knowledge of the position of the eyes in the head and the distribution of retinal cells allow to identify adaptive aspects of each species to its visual field, which is characteristic to the ecological niche it occupies. In this research, we study eye anatomical characteristics and retina neurochemical features of the rock cavy (Kerodon rupestris), a tipical Brazilian rodent from the suborder Hystricomorpha, family Caviidae. The rock cavy has lateral eyes well constitute bony orbit and well differentiated extrinsic muscle. The study of the descriptive and morphometric anatomy of the showed mean values of axial diameter 10.7±0,5mm and equatorial diameter 11.6±0.7mm. The pupil is slit shaped and the lens has mean axial diameter 5.4±0.03 mm, corresponding to ~45% of the axial diameter of the eye. The posterior nodal distance and the retinal magnification factor were estimated at 6.74 mm e 118 μm/grau, respectively. Flat mounts were processed for Nissl stain, and the topographic distribution of ganglion cells showed a moderate visual band, just below the optic disc, with higher density in the ventral retina. Retinal vertical sections and flat mounts were processed for immunohistochemistry to visualize tyrosine hydroxilase (TH) and thus two types of TH+ cells were detected. Type 1 cells had strong TH-immunoreactivity, the body cell varied from 120.047 to 269.373 μm2 stratifying in the sublamina 1 of the IPL. Type 2 cells were weakly TH-imunoreactive, had cell body located mostly in the IPL, varying from 54.848 to 177.142 μm2, constituting ~10% of the TH+ cells. Both cell types exhibited similar topographic distribution with higher density found in a horizontal band along of the naso-temporal axis in the dorsal retina. The total population of dopaminergic cells was 2,156±469,4 cells, occupying an average area of 198,164 μm2. The presence of cones and rods was detected by immunohistochemistry in vertical sections and flat mounts. S cones density is around 10 times smaller than L cones, with different degree of spatial organization. Other retinal neuronal populations of the rock cavy were also detected in vertical sections with specific markers. Comparative analysis of the anatomical characteristics of the rock cavy eye 12 suggest that it was designed to acquire higher sensitivity to light, at expense of image sharpness, compatible with a vision at mesopic conditions. Additionally, the distribution of the 2 subtypes of dopaminergic cells in a naso-temporal band in the dorsal retina seems suitable to a gain in sensitivity, coherent with an animal with predominantly crepuscular activity pattern

Efeitos da irrigação e adubação nitrogenada sobre a massa de forragem de cultivares de Panicum maximum Jacq.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)