Anormalidades fetais e placentárias decorrentes da produção in vitro de embriões bovinos

In vitro production of bovine embryo (IVP) has become a remarkable assisted reproduction biotechnology in Brazil, once it is considered an important tool for the genetic improvement of the herd. However, many abnormalities are associated with IVP technologies, such as higher incidences of embryo loss, abortions, hydrallantois and dystocia, prolonged gestation, increased birth weight and high perinatal mortality. Collectively, these abnormalities are known as “Large Offspring Syndrome”, which has limited the large-scale use of IVP technologies in cattle industry

Anestro pós-parto: luteólise prematura

The prolonged postpartum anoestrus in cows reduces the number of calves leading to a significant economic loss to producers. Suckling and nutrition are the factors of great importance to the extension of the post-partum period. Besides, the occurrence of short cycles within 30 to 40 days postpartum contributes to an increase in the parturition–conception interval. These cycles are related to development of a corpus luteum with reduced duration after the first ovulation (less than 12 days). It is known that the short persistence of the corpus luteum is caused by advance of the luteolytic mechanisms. However, there is a lack of consensus regarding the cause of this anticipation. There are two currently accepted hypotheses, one related to the lack of prior exposure to progesterone, and the other related to the low concentration of pre-ovulatory estrogen. Considering the decrease in the incidence of short cycles in cows treated with progesterone and estrogen, the main protocols of ovulation induction include combination of both hormones. Therefore, this study aimed at describing the post-partum anestrous in cows and the main predisposing factors, emphasizing the first postpartum ovulation, short cycle and, its respective causes and consequences

Investimento reprodutivo em fêmeas de populações sintópicas de Dendropsophus nanus (Boulenger, 1889) e D. sanborni (Schmidt, 1944) (Anura: Hylidae)

As espécies estudadas neste projeto, Dendropsophus nanus e Dendropsophus sanborni, são pererecas de pequeno porte, morfologicamente semelhantes e ocupam nichos similares. O presente trabalho foi realizado na Floresta Estadual Edmundo Navarro de Andrade, município de Rio Claro, estado de São Paulo e teve como objetivo obter informações acerca da biologia reprodutiva de ambas as espécies. Os principais resultados obtidos foram referentes à: (1) Relações de tamanho-fecundidade em fêmeas de D. nanus, sendo que foi significativa a correlação entre número de ovos e comprimento rostro-cloacal de fêmeas, o que não ocorreu para correlações entre tamanho dos ovos e CRC de fêmeas e entre tamanho e número de ovos, (2) Seleção do tamanho dos machos em D. nanus, com as fêmeas aparentemente acasalando com machos proporcionalmente mais leves e (3) Comportamento de desova de D. sanborni semelhante ao relatado para D. werneri, havendo dois tipos de interação parecidos: um envolvendo movimentos da cabeça e o outro das patas traseiras do macho, ambos descritos pela primeira vez para D. sanborni neste estudo.

Impacto da Mastite nos parâmetros reprodutivos em gado de leite

This study aimed to conduct a review in most articles relating mastitis and reproduction. Mastitis affects the reproduction according to its time of occurrence and agent. Mastitis affects the interval interestrus and also increases the number of days open, the number of services per conception, the abortion rate and decreases the conception rate. The inflammatory reaction generated by mastitis and all the toxins produced by bacteria alter the entire endocrine profile of cows that experienced episodes of mastitis and thus affect their reproduction

Estudos sobre os comportamentos reprodutivos e cuidados parentais em ciclídeos neotropicais

O estudo objetivou apresentar uma revisão bibliográfica sobre a filogenia, evolução, comportamentos reprodutivos e a evolução do cuidado parental em peixes da família Cichlidae, com ênfase em Cichlinae (ciclídeos Neotropicais). Atualmente a filogenia do grupo tem confirmado o monofiletismo da subfamília Etroplinae (India, Sri Lanka e Madagascar) como grupo irmão dos demais ciclídeos. Ptychochrominae (Madagascar) também monofilética é considerada grupo irmão dos clados Cichlinae (neotropicais) e Pseudocrenilabrinae (africanos), sendo os últimos clados irmãos entre si. Em relação às estratégias reprodutivas dos Cichlidae pode-se dividi-los quanto sua incubação em: Incubadores bucais (mouth-brooders), incubadores de substrato (substrate-spawners) e incubadores bucais tardios (mouth-brooders tardios), tais características podem nos oferecer embasamento para a compreensão das condições pelas quais as diferentes formas de cuidado parental e métodos de acasalamento evoluíram. O estudo enfatiza os comportamentos reprodutivos e cuidados parentais nos Cichlinae, descrevendo comportamentos que auxiliam na compreensão da biologia reprodutiva do grupo e sugerindo hipóteses sobre a origem e irradiação dos Cichlidae

História natural de Hypsiboas albopunctatus (Anura: Hylidae) na região de Rio Claro, estado de São Paulo

Natural history studies aim to know where the organisms live, what they do, and their relationships within the environment, including the other organisms. The anurans, among other vertebrates, exhibits the greatest variety of reproductive modes as well as a high complexity of social organization, that may enable a lot of naturalist studies. Anurans modes of organization are direct related to parental care, vocalization of chorus organization, and with males territoriality. In general, the social organization is influenced by species reproductive pattern. This study aim to get information about the natural history of Hypsiboas albopunctatus (perereca-cabrinha) a species of the family Hylidae, on the region of Rio Claro Municipality, São Paulo State, Brazil. The major aspects discussed were: seasonality, spatial distribution, acoustic and visual communication, reproductive mode, reproductive site, abiotic factors influences, territoriality, courtship behaviour, satellite male behavior, embrace and defensive behavior. To obtain this data, nocturnal field excursions were realized monthly. Two sites of Rio Claro county where chosen as studies sites, the sítio Cantaclaro (22o19’36’’S; 47o42’57’’O), on the District of Itapé and Floresta Estadual Edmundo Navarro de Andrade (FEENA) (22o24’58’’S; 47o31’26’’O).

Sanidade Uterina Pós Parto: Aspectos Imunológicos e Terapêuticos

Function of the uterus is often compromised in cattle by bacterial contamination of the uterine lumen after parturition. Pathogenic bacteria often persist, causing uterine disease, a major cause of infertility in cattle. Knowledge of the immunological aspects of the uterus involved in maintaining reproductive healthiness is fundamental to the study of uterine infections that affect the uterus postpartum. Polymorphonuclear leukocytes have an important role because they are the first line of defense against colonization of bacteria in utero (Hammon and Goff, 2006). The establishment of uterine infection depends in parts of endocrine environment, particularly progesterone, which suppresses the immune system (Lewis, 2003). In the puerperium may occur uterine disorders, such as retained placenta, puerperal metritis, clinical and subclinical endometritis and pyometra, this review was proposed a study of the immunology involved in uterine health and a better understanding of uterine disorders, using the model of Sheldon et al. al, (2006) for classification of diseases, and a study of best treatment options and discussion about its functionality, because a lot of controversy among authors about choosing a treatment and another and between treated and untreated

Influência da luz LED em culturas de células-tronco mesenquimais in-vitro

Stem cells are defined as cells capable of self-renewal and differentiation into specialized cells when submited to external signalings in the enviroment. Among adult stem cells, mesenchymal cells occupy an important position because they can differentiate into mesodermal cells such as osteoblasts, adipocytes and chondrocytes. Cell therapy consists in the use of mesenchymal stem cells (MSC) in the treatment of degenerative diseases and harmed tissue reconstruction. Due to the longstanding and costly procedure for cultivation of MSC, it was proposed the use of low power light sources, such as light emitting diodes (LED), to optimize these factors. Recent works have shown a series of results from the influence of LED light on biological tissues such as increased rate of cell proliferation, increased RNA, DNA and ATP synthesis rate. The purpose of this study is to compare the biomodulator effect of LED light set at wavelengths 630nm ± 10nm and 805nm ± 10nm on the mesenchymal stem cells proliferation. For this, the mesenchymal stem cells culture adopted the procedure used in the Departament of Animal Reproduction and Veterinary Radiology of the Faculty of Veterinary Medicine and Animal Sciences of Botucatu. MSC were obtained from an adult horse bone marrow, and isolated by density gradient separation, with the FICOLL reagent and by centrifugation. The pellet containing the stem cells was removed and these were placed in low glucose DMEM culture medium, containing 10% fetal calf serum and antibiotics. The material was observed daily by inverted microscopy for monitoring the progression of the cells and subsequently the amount of cells were counted in a Neubauer counting chamber. The amount of MSC was obtained by cell culture seeded in 24 wells culture plate and segregated into three distinct groups: Group 1 was irradiated with wavelength set at 630nm ± 10 nm, Group... (Complete abstract click electronic access below)

Efeitos de diferentes fontes de gordura alimentar na resposta a aspiração folicular de cabras anglo-nubianas

Pós-graduação em Medicina Veterinária - FCAV

Características dos espermatozóides em espécies de Leptodactylus (Anura, Leptodactylidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Área de vida e biologia reprodutiva da maria-da-restinga (Phylloscartes kronei) (Aves, Tyrannidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da corticoterapia pré-natal e durante a puberdade sobre a morfofisiologia do lobo ventral da próstata de ratos senis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expressão gênica e potencial de desenvolvimento in vitro dos complexos cúmulos-oócitos ovinos tratados com roscovitina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Utiização de altrenogest injetável par o estabelecimento e manutençao da gestação em éguas receptoras de embriões acíclicas

Pós-graduação em Medicina Veterinária - FMVZ

Características seminais e resfriamento de sêmen de tamanduá (Myrmecophaga tridactyla) de vida livre

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)