Bag and size limit analyses for red drum in Northern and Southern Regions of the U.S. Atlantic

Assessments of the Atlantic red drum for the northern (North Carolina and north) and southern (South Carolina through east coast of Florida) regions along the U. S. Atlantic coast were recently completed. The joint Red Drum Technical Committee (SAFMC/ASMFC) selected the most appropriate catch matrix (incorporating an assumption on size of recreationally-released fish), selectivity of age 3 relative to age 2, and virtual population analysis (FADAPT). Given gear- and age-specific estimates of fishing mortality (F) for the 1992-1998 period, analyses were made of potential gains in escapement through age 4 and static spawning potential ratio (SPR) from further reductions in fishing mortality due to changes in slot and bag limits. Savings from bag limits were calculated given a particular slot size for the recreational fishery, with no savings for the commercial fisheries in the northern region due to their being managed primarily through a quota. Relative changes in catch-at-age estimates were used to adjust age-specific F and hence calculated escapement through age 4 and static SPR. Adjustment was made with the recreational savings to account for release mortality (10%, as in the stock assessment). Alternate runs for the northern region commercial fishery considered 25% release mortality for lengths outside the slot (instead of 0% for the base run), and 0% vs. 10% gain or loss across legal sizes in F. These results are summarized for ranges of bag limits with increasing minimum size limit (for fixed maximum size), and with decreasing maximum size limit (for fixed minimum size limit). For the southern region, a bag limit of one-fish per angler trip would be required to attain the stated target of 40% static SPR if the current slot limit were not changed. However, for the northern region, a bag limit of one-fish per angler trip appears to be insufficient to attain the stated target of 40% static SPR while maintaining the current slot limit. (PDF contains 41 pages)

Hydroacoustic evaluation of spawning red hind (Epinephelus guttatus) aggregations along the coast of Puerto Rico in 2002 and 2003

Red hind (Epinephelus guttatus) have been overfished in the Caribbean and were included with seven other regional grouper species deemed vulnerable to risk of extinction. The Puerto Rico Department of Natural and Environmental Resources desired to map spawning red hind aggregations within commonwealth waters as part of their resource management program for the species. Mobile hydroacoustic surveys were conducted over 3-day periods in 2002 and 2003, indexed to the full moon phase in February or March when red hind were known to aggregate. Four vessels concurrently sampled the southwest, south, and southeast coasts of Puerto Rico in 2002. In 2003, three vessels conducted complementary surveys of the northwest, north, and northeast coasts of the island, completing a circuit of the coastal shelf-spawning habitat. These surveys indicated that red hind spawning aggregations were prevalent along the south and west coasts, and sparse along the north coast during the survey periods. Highest spawning red hind concentrations were observed in three areas offshore of the west coast of Puerto Rico, around Mona and Desecheo islands (20,443 and 10,559 fish/km2, respectively) and in the Bajo de Cico seasonal closed area (4,544 fish/km2). Following both 2002 and 2003 surveys, a series of controlled acoustic measurements of known local fish species in net pens were conducted to assess the mean target strength (acoustic backscatter) of each group. Ten species of fish were measured, including red hind (E. guttatus), coney (E. fulvus), white grunt (Haemulon plumieri), pluma (Calamus pennatula), blue tang (Acanthurus coeruleus), squirrel fish (Holocentrus spp.), black durgeon (Melichtyhs niger), ocean file fish (Canthidermis sufflamen), ocean surgeon fish (Acanthurus bahianus), and butter grouper (Mycteroperca spp.). In general, the mean target strength results from the caged fish experiments were in agreement with published target strength length relationships, with the exception of white grunt and pluma.

Desarrollo de una aplicación para una red de sensores inalámbricos (WSN) mediante Contiki

Durante los últimos años hemos venido observando la tendencia a incorporar capacidad de proce- samiento y comunicación a dispositivos que hasta entonces se utilizaban de modo independiente. La evolución de los móviles a smartphones es un claro ejemplo de dicha tendencia, aunque también cabe mencionar otros ejemplos, como es el caso de los denominados hogares inteligentes, en los que elementos del hogar se encuentran interconectados y pueden actuar de modo conjunto. Esta ten- dencia no se limita a sistemas independientes, sino que propone interconectar todos los elementos disponibles para conformar la denominada Internet de los Objetos/Cosas o Internet of Things, IoT. Una de las mayores dificultades que se presenta en estos sistemas es que las características de es- tos nuevos dispositivos inteligentes, en general limitados en términos de cómputo, almacenamiento, autonomía o comunicación, queda a menudo lejos de los equipos informáticos tradicionales. Esta cuestión, junto con la ausencia de estándares para gestionar estos dispositivos, constituye un impor- tante problema a abordar. Considerando este marco, en este proyecto se ha desarrollado una aplicación orientada a este tipo de dispositivos. Más concretamente, la aplicación tiene como soporte una red de sensores inalámbricos, WSN, con el objetivo de realizar seguimiento de individuos. Cabe destacar que el desarrollo de la aplicación se ha realizado utilizando Contiki OS, sistema ope- rativo diseñado especialmente para dispositivos con características limitadas como los presentados anteriormente y firme candidato a convertirse en estándar.

Soporte a la explotación de datos y gestión de una red de sensores

El seguimiento de distintas especies de animales contribuye en gran medida a su estudio y, por tanto, a su conservación y control. Los avances tecnológicos de los últimos años han facilitado las posibilidades de seguimiento con la creación de distintos dispositivos que permiten conocer los movimientos de la especie que se desea estudiar. Uno de los sistemas más utilizados consiste en la utilización de dispositivos GPS incorporados al espécimen sobre el que se realiza el seguimiento y cuya señal es recogida por satélites que se encargan de almacenar y posteriormente reenviar la información para su almacenamiento y procesamiento en el laboratorio. El principal problema de este sistema es su elevado coste. Existen alternativas que no presentan un coste tal alto, tales como el uso de módulos basados en telefonía móvil. Sin embargo, tienen limitaciones de cobertura, por lo que no es aplicable en todos los ámbitos. Este proyecto forma parte de una propuesta que ofrece realizar seguimiento de ejemplares de una especie de ave, la gaviota Patiamarilla, en Gipuzkoa mediante la utilización de una red de sensores y que tiene varias ventajas frente a las opciones presentadas anteriormente. En este proyecto en concreto se ha diseñado e implementado el módulo que permite recoger la información obtenida por el conjunto de sensores (cada ejemplar lleva incorporado un sensor que permite registrar su posición) y enviarla a un servidor centralizado para su posterior consulta y análisis. Adicionalmente, también se permite consultar el último estado registrado de cada dispositivo de seguimiento, además de contemplar la posibilidad de actualizar su software.

Red-shift conical emission by femtosecond pulses at low input power

Red-shift conical emission (CE) is observed by femtosecond laser pulse propagating in BK7 at a low input power (compared to those input powers for generation of blue-shift CE). With the increasing input power the blue-shift CE begins to appear whereas the red-shift CE ring (902 nm in our experiment) disappears accompanied by the augment of the central white spot size synchronously. The disappearing of red-shift CE in our experiment is explained such that the increase of axial intensity is much higher than that of ring emission and the augment of the central white spot size with the increasing input laser power.

Red shift and broadening of backward harmonic radiation from electron oscillations driven by femtosecond laser pulse

The characteristics of backward harmonic radiation due to electron oscillations driven by a linearly polarized fs laser pulse are analysed considering a single electron model. The spectral distributions of the electron's backward harmonic radiation are investigated in detail for different parameters of the driver laser pulse. Higher order harmonic radiations are possible for a sufficiently intense driving laser pulse. We have shown that for a realistic pulsed photon beam, the spectrum of the radiation is red shifted as well as broadened because of changes in the longitudinal velocity of the electrons during the laser pulse. These effects are more pronounced at higher laser intensities giving rise to higher order harmonics that eventually leads to a continuous spectrum. Numerical simulations have further shown that by increasing the laser pulse width the broadening of the high harmonic radiations can be controlled.

Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy

The effect of alcohol solution on single human red blood Cells (RBCs) was investigated using near-infrared laser tweezers Raman spectroscopy (LTRS). In our system, a low-power diode laser at 785 nm was applied for the trapping of a living cell and the excitation of its Raman spectrum. Such a design could simultaneously reduce the photo-damage to the cell and suppress the interference from the fluorescence on the Raman signal. The denaturation process of single RBCs in 20% alcohol solution was investigated by detecting the time evolution of the Raman spectra at the single-cell level. The vitality of RBCs was characterized by the Raman band at 752 cm(-1), which corresponds to the porphyrin breathing mode. We found that the intensity of this band decreased by 34.1% over a period of 25 min after the administration of alcohol. In a further study of the dependence of denaturation on alcohol concentration, we discovered that the decrease in the intensity of the 752 cm(-1) band became more rapid and more prominent as the alcohol concentration increased. The present LTRS technique may have several potential applications in cell biology and medicine, including probing dynamic cellular processes at the single cell level and diagnosing cell disorders in real time. Copyright (c) 2005 John Wiley T Sons, Ltd.

Diseño y despliegue de una red inalámbrica segura y control de acceso de sensores IP

[ES]El proyecto está orientado a conseguir una comunicación inalámbrica y segura de una red de sensores IP. Por un lado, mediante el protocolo 6LoWPAN se consigue que los datos se transmitan mediante IPv6 y, por otro lado, gracias al protocolo LADON se establecen los servicios de seguridad de autenticación, integridad de datos, autorización y control de acceso.

Computationally Guided Monomerization of Red Fluorescent Proteins of the Class Anthozoa

Red fluorescent proteins (RFPs) have attracted significant engineering focus because of the promise of near infrared fluorescent proteins, whose light penetrates biological tissue, and which would allow imaging inside of vertebrate animals. The RFP landscape, which numbers ~200 members, is mostly populated by engineered variants of four native RFPs, leaving the vast majority of native RFP biodiversity untouched. This is largely due to the fact that native RFPs are obligate tetramers, limiting their usefulness as fusion proteins. Monomerization has imposed critical costs on these evolved tetramers, however, as it has invariably led to loss of brightness, and often to many other adverse effects on the fluorescent properties of the derived monomeric variants. Here we have attempted to understand why monomerization has taken such a large toll on Anthozoa class RFPs, and to outline a clear strategy for their monomerization. We begin with a structural study of the far-red fluorescence of AQ143, one of the furthest red emitting RFPs. We then try to separate the problem of stable and bright fluorescence from the design of a soluble monomeric β-barrel surface by engineering a hybrid protein (DsRmCh) with an oligomeric parent that had been previously monomerized, DsRed, and a pre-stabilized monomeric core from mCherry. This allows us to use computational design to successfully design a stable, soluble, fluorescent monomer. Next we took HcRed, which is a previously unmonomerized RFP that has far-red fluorescence (λemission = 633 nm) and attempted to monomerize it making use of lessons learned from DsRmCh. We engineered two monomeric proteins by pre-stabilizing HcRed’s core, then monomerizing in stages, making use of computational design and directed evolution techniques such as error-prone mutagenesis and DNA shuffling. We call these proteins mGinger0.1 (λem = 637 nm / Φ = 0.02) and mGinger0.2 (λem = 631 nm Φ = 0.04). They are the furthest red first generation monomeric RFPs ever developed, are significantly thermostabilized, and add diversity to a small field of far-red monomeric FPs. We anticipate that the techniques we describe will be facilitate future RFP monomerization, and that further core optimization of the mGingers may allow significant improvements in brightness.

Mechanism of red colouration in Tovel-See (”Conclusions” only) [Translation from: Archiv fur Hydrobiologie 38 299-302, 1941]

Cases of red colouration in small lake basins, due to the abundant appearance of microorganisms have long been known. Usually it is caused by a fast, sudden, intensive propagation (so called ”bloom”) of Cyanophycae and bacteria. (e.g. Oscillatoracae, thiobacteria etc.). An exception to this is the red colouration of Tovel-See, an alpine lake basin in the Dolomites of the Brenta group (Trentino), lying at a height of 1178 m and hidden in the woodland of a valley. Here the red bloom has a double rhythm: a daily and a yearly rhythm. The colouration of one part of the lake takes place in the warmest months of the year (i.e. July, August, September) and in the middle hours of the day. The immediate origin of the bloom has been known for a long time: it is caused by the Peridinacae Glenodinium sanguineum. This paper describes the phenomenon of red colouration of the lake and discusses its conditions.

Diseño de un detector para una red de vigilancia radiológica ambiental

[ES]El objetivo de este trabajo es analizar el montaje de un contador proporcional de radiación para su uso en una red de vigilancia radiológica ambiental desde su diseño básico hasta su puesta en marcha. Se realizará un análisis de las razones que nos han llevado a elegir este tipo de contadores de ionización gaseosa (frente a otros tipos), para llegar a la conclusión de que son los más adecuados para la vigilancia radiológica ambiental. Este análisis describirá la solución a la que se ha llegado para construir un contador con los medios al alcance y el porqué de su diseño concreto, así como la metodología de trabajo que se ha seguido para la obtención del mismo. Basándonos en suposiciones teóricas derivadas del análisis geométrico de la instrumentación para la obtención de resultados previsibles, se realizará una verificación mediante ensayos técnicos en el laboratorio. Se presupuestará el material utilizado y las horas requeridas, para llevar una cuenta de lo que le ha costado al departamento y lo que le habría costado en caso de no poseer la instrumentación utilizada. Después de realizar diversos test relacionados con la instrumentación electrónica, se procederá a obtener unas conclusiones derivadas de los ensayos en el laboratorio para su posterior comparación con las estimaciones teóricas. En el anexo I se encuentran los planos de diseño que se han realizado y en el anexo II los resultados finales obtenidos de los ensayos.

Diseño y desarrollo de una interfaz de configuración de un generador de tráfico de red

[ES]Se trata de un software de gobierno para un inyector de tráfico de altas prestaciones capaz de adaptar el tráfico de red a distintos parámetros. Además, se deberán integrar el conjunto en la arquitectura de pruebas del laboratorio.

Diseño electrónico de una red de comunicaciones para monitorización de estructuras

[ES]El Trabajo Fin de Grado “Diseño electrónico de una red de comunicaciones para monitorización de estructuras” consiste en el desarrollo de un sistema de comunicación orientado al despliegue y gestión de una red con un alto número de sensores para monitorización de estructuras a bordo de una aeronave. El objetivo principal es hacer viable la utilización de dicha red, partiendo de la premisa de crear una alternativa con mayor eficiencia energética que los sistemas actualmente disponibles para tal fin.

Reposición de la Red Básica Municipal de Donostia/San Sebastián

En el presente Trabajo Fin de Grado, se trabajará sobre la Red Básica Municipal de Donostia/San Sebastián, donde el autor ha sido partícipe en la campaña de reposición de la Red. Nos remontaremos a los orígenes de la Red, explicando cómo nació la Red Básica Municipal de Donostia/San Sebastián y la metodología que se usó para los trabajos previos a su implantación. A lo largo del trabajo, se explicará la metodología de observación y cálculo que se ha empleado en la actual reposición de la Red, el cual ha sido desarrollado por la empresa Geograma S.L. para el Ayuntamiento de San Sebastián entre enero y mayo de 2014. Finalmente, se estudiará como metodología alternativa las observaciones RTK en Red (NRTK), con el fin de validar el método para trabajos de observación de Redes Urbanas de Referencias Topográficas.