Androgen receptor targets NFKB and TSPI to suppress prostate tumor growth in vivo

The androgen role in the maintenance of prostate epithelium is subject to conflicting opinions. While androgen ablation drives the regression of normal and cancerous prostate, testosterone may cause both proliferation and apoptosis. Several investigators note decreased proliferation and stronger response to chemotherapy of the prostate cancer cells stably expressing androgen receptor (AR), however no mechanistic explanation was offered. In this paper we demonstrate in vivo anti-tumor effect of the AR on prostate cancer growth and identify its molecular mediators. We analyzed the effect of AR on the tumorigenicity of prostate cancer cells. Unexpectedly, the AR-expressing cells formed tumors in male mice at a much lower rate than the AR-negative controls. Moreover, the AR-expressing tumors showed decreased vascularity and massive apoptosis. AR expression lowered the angiogenic potential of cancer cells, by increasing secretion of an anti-angiogenic protein, thrombospondin-1. AR activation caused a decrease in RelA, a subunit of the pro-survival transcription factor NF kappa B, reduced its nuclear localization and transcriptional activity. This, in turn, diminished the expression of its anti-apoptotic targets, Bcl-2 and IL-6. Increased apoptosis within AR-expressing tumors was likely due to the NF kappa B suppression, since it was restricted to the cells lacking nuclear (active) NF kappa B. Thus we for the first time identified combined decrease of NF kappa B and increased TSP1 as molecular events underlying the AR anti-tumor activity in vivo. Our data indicate that intermittent androgen ablation is preferable to continuous withdrawal, a standard treatment for early-stage prostate cancer. (C) 2007 Wiley-Liss, Inc.

Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle

Background: The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression. Methodology/Principal Findings: A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation. Conclusions/Significance: Of the,27,000 predicted open reading frames, transcripts homologous to only,5900 are not detectable in any of these life cycle stages, indicating that this single-celled organism does indeed contain a large number of functional genes. Transcripts from over 5000 predicted genes are expressed at levels >5x corrected background and 95 genes are expressed at >250x corrected background in all stages. Transcripts homologous to 91 predicted genes are specifically expressed and 155 more are highly up-regulated in growing cells, while 90 are specifically expressed and 616 are up-regulated during starvation. Strikingly, transcripts homologous to 1068 predicted genes are specifically expressed and 1753 are significantly up-regulated during conjugation. The patterns of gene expression during conjugation correlate well with the developmental stages of meiosis, nuclear differentiation and DNA elimination. The relationship between gene expression and chromosome fragmentation is analyzed. Genes encoding proteins known to interact or to function in complexes show similar expression patterns, indicating that co-ordinate expression with putative genes of known function can identify genes with related functions. New candidate genes associated with the RNAi-like process of DNA elimination and with meiosis are identified and the late stages of conjugation are shown to be characterized by specific expression of an unexpectedly large and diverse number of genes not involved in nuclear functions.

A produção de vinhos em regiões tropicais: anais.

Características dos solos agrícolas da região semi-árida do Vale do São Francisco. Bases de dados georreferenciadas para a seleção de áreas vitícolas do Vale do Submédio São Francisco . O clima vitícola do Submédio São Francisco e o zoneamento dos períodos de produção de uvas para elaboração de vinhos. Variabilidade espacial do clima no Vale do Submédio São Francisco com vistas ao zoneamento mesoclimático . Zonificación de terroir aplicado a zonas sin tradición vitícola: el caso del Valle Submedio del Río San Francisco (Pernambuco, Brasil). Comportamiento fisiológico de la vid en climas cálidos y en particular durante el período de maduración de la uva. Técnicas de produção vitícola com ciclos sucessivos em condições tropicais. Análise dos atuais sistemas de produção de uvas para vinho no Vale do São Francisco. Introdução e avaliação de novas cultivares para vinho no Vale do São Francisco. Manejo de água na cultura da videira. Problemas fitossanitários na viticultura do Vale do São Francisco . Problemas entomológicos na viticultura do Vale do São Francisco. Selección de cultivares y desarrollo de nuevos viños de calidad en climas cálidos: comportamiento agronómico y enológico en la región de jerez, España. Estabilidade da matéria corante dos vinhos de regiões de clima quente. Peculiaridades de la vinificación de uvas producidas en climas cálidos. Diferenciação da composição fenólica de vinhos portugueses induzida por colagens protéicas. Características das uvas do Vale do São Francisco sob o ponto de vista enológico. Características analíticas e sensoriais de vinhos produzidos no Vale do Submédio São Francisco, Brasil. Desafios e prioridades para o desenvolvimento científico e tecnológico da produção de vinhos de qualidade em zonas tropicais.

Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light

Chungui Lu, Olga A. Koroleva, John F. Farrar, Joe Gallagher, Chris J. Pollock, and A. Deri Tomos (2002). Rubisco small subunit, chlorophyll a/b-binding protein and sucrose : fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light. Plant Physiology, 130 (3) pp.1335-1348 Sponsorship: BBSRC RAE2008

Nowe metody wyznaczania parametru asymetrii tensora gradientu pola elektrycznego w spektroskopii NQR i ich zastosowanie do badania układów nieuporządkowanych

Wydział Fizyki

Konstrukcje awansu społecznego w Stanach Zjednoczonych. Między orientacją na dyplom uniwersytecki a sukcesem medialnym

Wydział Studiów Edukacyjnych

Egzystencjalna autonomia człowieka a świat wartości duchowych według Lwa Tołstoja

Wydział Teologiczny

Informe final asesoría en diseño : carpintería y trabajos en tagua Chiquinquirá Boyacá y cestería en esparto Cerinza Boyacá, Bogotá desarrollo de producto.

165 hojas : fotografías, cuadros.

Laboratorio Colombiano de Diseño para la Artesanía y la Pequeña Empresa : modulo taller fundamentos del diseño aplicado a la artesanía.

39 hojas : ilustraciones.

Carpintería y trabajos en tagua. Chiquinquirá (Boyacá). Desarrollo de producto.

139 hojas : ilustraciones.

Cestería en esparto - Cerinza (Boyacá) Etapa producción y socialización.

38 hojas : fotografías, cuadros.

Informe Final : Asesoría en Diseño Gráfico.

56 hojas : ilustraciones, cuadros.

Diagnóstico del trabajo artesanal en concha de nácar. Santa Marta - Magdalena.

51 hojas : fotografías.

Asesoria de diseño para producción de líneas de candelabros.

86 hojas : ilustraciones, fotografías a color.

Cestería en Tumaco : cuaderno de diseño

31 hojas: ilustraciones, fotografías a color.