PLANT-TO-SEED TRANSMISSION of CURTOBACTERIUM FLACCUMFACIENS pv. FLACCUMACIENS IN A DRY BEAN CULTIVAR

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pathogenicity of Curtobacterium flaccumfaciens pv. flaccumfaciens to several plant species

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparação de dois métodos de extração de Xanthomonas campestris pv. phaseoli em sementes de feijoeiro

The objective of this research was to evaluate the effectiveness of the extraction of Xanthomonas campestris pv. phaseoli from naturally infected dry bean seeds. Extraction methods tested included soaking whole seeds in sterilized saline phosphate buffer and crushing seeds after soaking in sterilized saline phosphate buffer. The bacterium was isolated on a semiselective agar medium. The seed crushing method was found to be more effective. The bacterium strains isolated were pathogenic to bean leaves, reacted with X. campestris pv. phaseoli antiserum, and had morphological and physiological/biochemical characteristics typical of the X. campestris pv. phaseoli.

Virulência de isolados Dexanthomonas campestris pv. Phaseoli (Smith) dye em feijoeiro

The objective of this research was to evaluate de reaction of leaves and pods of five cultivars of bean (Phaseolus vulgaris L.) plants to twenty strains of Xanthomonas campestris pv. phaseoli. The strains were inoculated onto leaves in a greenhouse, and onto pods in a growth chamber. The results obtained were analyzed and the strains classified into three groups: low, medium, and high virulence. Most of the strains showed high virulence on leaves of Carioca and Rio Negro cultivars, as opposed to only low to medium virulence on leaves of IAPAR 14, IAPAR 16, and G. N. Nebraska # 1 sel. 27 cultivars. There were, however, individual strains powerful enough to overcome the leaf resistance of IAPAR 14, IAPAR 16, and G. N. Nebraska # 1 sel. 27. With regard to pods, most strains showed high virulence on all bean cultivars, with exception of IAPAR 14 where virulence was at medium level. A correlation between leaf and pod symptoms was found to exist in Carioca, Rio Negro, and IAPAR 14 cultivars. No such correlation was observed in IAPAR 16 and G. N. Nebraska # 1 sel. 27. Comparing strains producing melanine in vitro with those not producing this pigment, no difference was observed with regard to virulence.

Inverter-fed linear asynchronous machine

The behaviors of an arc-shaped stator induction machine (the sector-motor) and a disc-secondary linear induction motor are analyzed in this work for different values of the frequency. Variable frequency is produced by a voltage source controlled-current inverter which keeps constant the r.m.s. value of the phase current, also assuring a sinusoidal waveform. For the simulations of the machine developed thrust, an equivalent circuit is used. It is obtained through the application of the one-dimensional theory to the modeling. The circuit parameters take into account the end effects, always present is these kind of machines. The phase current waveforms are analyzed for their harmonic contents. Experimental measurements were carried out in laboratory and are presented with the simulations, for comparison.

Reação de genótipos de feijoeiro comum a Fusarium oxysporum f. sp. phaseoli, Macrophomina phaseolina e Xanthomonas campestris pv. phaseoli

The behavior of dry bean (Phaseolus vulgaris L.) genotypes PI 150414, PI 163117, PI 175829 white, PI 175829 purple, PI 175858, PI 197687, A 417, A 420, A 429, Xan 160, Xan 161, WISHBR 40, and IAC Carioca inoculated with Fusarium oxysporum f. sp. phaseoli, Macrophomina phaseolina, and Xanthomonas campestris pv. phaseoli was evaluated under greenhouse condition. The bean genotypes Xan 160, PI 150414, A 417, PI 175829 purple, Xan 161, A 420, PI 163117, and PI 175829 white were resistant to F. oxysporum f. sp. phaseoli, and only PI 155829 white had a good level of resistance to M. phaseolina. All bean genotypes were susceptible to Feij-4 strain, and only Xan 161 had some level of leaf resistance to Feij-41 strain of X. campestris pv. phaseoli.

Semi-selective culture medium for Xanthomonas axonopodis pv. malvacearum detection in cotton seeds (Gossypium hirsutum L.)

The cotton disease known as angular leaf spot, caused by Xanthomonas axonopodis pv. malvacearum (Xam) has been causing cotton losses in several producing regions around the world. Xam is transmitted by seeds, which may be infected both externally and internally. Infected seeds constitute the main long-distance dissemination mode of the pathogen. In view of this, the use of healthy seeds is a must. To accomplish that, detection methodologies for the bacteria must be developed be used in seed health analysis laboratories. This study aimed to develop a semi-selective medium for Xam detection in cotton seeds. The semi-selective culture medium was named MSSXAN and it was consisted of peptone (5.0 g), beef extract (3 g), sucrose (5 g), soluble starch (10 g), agar (15 g), CaCl 2 (0.25 g), Tween 80 (10 mL), distilled water (1,000 mL), crystal violet solution at 1% (150 μL), cephalexin (50 mg 1*), methyl thyophanate (10 mg*) and chlorothalonil (10 mg*) - *added after culture medium autoclaving. This MSSXAN medium shows low repressiveness to Xam and it be used for isolation of this bacteria in cotton seeds health analysis. © 2009 Academic Journals Inc.

Development and validation of a Xanthomonas axonopodis pv. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome

Background. From shotgun libraries used for the genomic sequencing of the phytopathogenic bacterium Xanthomonas axonopodis pv. citri (XAC), clones that were representative of the largest possible number of coding sequences (CDSs) were selected to create a DNA microarray platform on glass slides (XACarray). The creation of the XACarray allowed for the establishment of a tool that is capable of providing data for the analysis of global genome expression in this organism. Findings. The inserts from the selected clones were amplified by PCR with the universal oligonucleotide primers M13R and M13F. The obtained products were purified and fixed in duplicate on glass slides specific for use in DNA microarrays. The number of spots on the microarray totaled 6,144 and included 768 positive controls and 624 negative controls per slide. Validation of the platform was performed through hybridization of total DNA probes from XAC labeled with different fluorophores, Cy3 and Cy5. In this validation assay, 86% of all PCR products fixed on the glass slides were confirmed to present a hybridization signal greater than twice the standard deviation of the deviation of the global median signal-to-noise ration. Conclusions. Our validation of the XACArray platform using DNA-DNA hybridization revealed that it can be used to evaluate the expression of 2,365 individual CDSs from all major functional categories, which corresponds to 52.7% of the annotated CDSs of the XAC genome. As a proof of concept, we used this platform in a previously work to verify the absence of genomic regions that could not be detected by sequencing in related strains of Xanthomonas. © 2010 Moreira et al; licensee BioMed Central Ltd.

Inversor CS Boost monofásico em aplicações com fontes renováveis

This paper presents a new methodology for the operation and control of a single-phase current-source (CS) Boost Inverter, considering that the conventional current-source inverter (CSI) has a right-half-plane (RHP) zero in its control-to-output transfer function, and this RHP zero causes the known non-minimum-phase effects. In this context, a special design with low boost inductance and a multi-loop control is developed in order to assure stable and very fast dynamics. Furthermore, the Inverter presents output voltage with very low total harmonic distortion (THD), reduced components and high power density. Therefore, this paper presents the inverter operation, the proposed control technique, and main simulation and experimental results in order to demonstrate the feasibility of the proposal. © 2010 IEEE.

Single-phase current-source-boost inverter for renewable energy sources

This paper presents a new methodology for the operation and control of a single-phase current-source (CS) Boost Inverter, considering that the conventional CS boost inverter has a right-half-plane (RHP) zero in its control-to-output transfer function, and this RHP zero causes the known non-minimum-phase effects. In this context, a special design with low boost inductance and a multi-loop control is developed in order to assure stable and very fast dynamics. Furthermore, the proposed inverter presents output voltage with very low total harmonic distortion (THD), reduced components and high power density. Therefore, this paper presents the inverter operation, the proposed control technique, the main simulation results and a prototype in order to demonstrate the feasibility of the proposal. © 2011 IEEE.

Comparative analysis of MPPT techniques for PV applications

This paper presents a careful evaluation among the most usual MPPT techniques, doing meaningful comparisons with respect to the amount of energy extracted from the photovoltaic (PV) panel, PV voltage ripple, dynamic response and use of sensors, considering that the models are first implemented via MatLab/Simulink®, and after a digitally controlled boost DC-DC converter was implemented and connected to an Agilent Solar Array simulator in order to verify the simulation results. The prototype was built, the algorithms are digitally developed and the main experimental results are also presented, including dynamic responses and the experimental tracking factor (TF) for the analyzed MPPT techniques. © 2011 IEEE.

Modeling of a MIT for the application of a frequency inverter of the electric vehicle

Nowadays, drives that use a combination of induction motors and frequency inverters are very common, a fact due to the financial practicality and viability in purchasing and operating that system. This system modeling and simulation becomes important when it wants to evaluate the performance, to calculate and correct parameters, and it has a fundamental role in functionality and viability analysis for application of new configurations and technologies. This work is about to elaborate a simple induction motor model based in the torque versus speed characteristic, using the linearization method for application in a specific operation range to be controlled by a frequency inverter. © 2011 Springer-Verlag Berlin Heidelberg.

Research on photovoltaics: Review, trends and perspectives

This paper presents a briefly review, some trends and perspectives in the field of Photovoltaic energy conversion, which is considered to be the most important renewable energy source in few years, in the coming decades. The power electronics plays a fundamental role in this process, developing systems each times more competitive, efficient, reliable, and also reducing costs and reducing the payback time. Some trends are visible, which are the use of Silicon Carbide devices in PV inverters, the use of integrated inverter structures, the integration of power converters into the PV module or the use of few PV series connection, the development of thinner and more efficient solar cells. Moreover, the discussion about the necessity of MPPT and anti-island schemes are presented, mainly considering the expected growth of grid-tied applications. © 2011 IEEE.

3-D Space Vector PWM implementation for Four-Leg Voltage Source Inverter

The Space Vector PWM implementation and operation for a Four-leg Voltage Source Inverter (VSI) is detailed and discussed in this paper. Although less common, four-leg VSIs are a viable solution for situations where neutral connection is necessary, including Active Power Filter applications. This topology presents advantages regarding the VSI DC link and capacitance, which make it useful for high power devices. Theory, implementation and simulations are also discussed in this paper. © 2011 IEEE.