Conservation of synteny between the genome of the pufferfish (Fugu rubripes) and the region on human chromosome 14 (14q24.3) associated with familial Alzheimer disease (AD3 locus)

The genome of the pufferfish (Fugu rubripes) (400 Mb) is approximately 7.5 times smaller than the human genome, but it has a similar gene repertoire to that of man. If regions of the two genomes exhibited conservation of gene order (i.e., were syntenic), it should be possible to reduce dramatically the effort required for identification of candidate genes in human disease loci by sequencing syntenic regions of the compact Fugu genome. We have demonstrated that three genes (dihydrolipoamide succinyltransferase, S31iii125, and S20i15), which are linked to FOS in the familial Alzheimer disease focus (AD3) on human chromosome 14, have homologues in the Fugu genome adjacent to Fugu cFOS. The relative gene order of cFOS, S31iii125, and S20i15 was the same in both genomes, but in Fugu these three genes lay within a 12.4-kb region, compared to >600 kb in the human AD3 locus. These results demonstrate the conservation of synteny between the genomes of Fugu and man and highlight the utility of this approach for sequence-based identification of genes in human disease loci.

Molecular markers reveal cryptic sex in the human pathogen Coccidioides immitis.

Coccidioides immitis, cause of a recent epidemic of "Valley fever" in California, is typical of many eukaryotic microbes in that mating and meiosis have yet to be reported, but it is not clear whether sex is truly absent or just cryptic. To find out, we have undertaken a population genetic study using PCR amplification, screening for single-strand conformation polymorphisms, and direct DNA sequencing to find molecular markers with nucleotide-level resolution. Both population genetic and phylogenetic analyses indicate that C. immitis is almost completely recombining. To our knowledge, this study is the first to find molecular evidence for recombination in a fungus for which no sexual stage has yet been described. These results motivate a directed search for mating and meiosis and illustrate the utility of single-strand conformation polymorphism and sequencing with arbitrary primer pairs in molecular population genetics.

The Thelephoraceae of North America.

8-12

Indenture acknowledging sale of property, Boston, 1753

Document acknowledges the sale of the late Samuel Clark's house and property to Alexander Hill. Samuel Clark's executor, James Clark, was required by law to sell the property to the highest bidder in order to pay the debts of the deceased.

University of Michigan Women's Cross Country Team, 1983

Back Row: captain Judy Yuhn, Sue Schroeder, Jennifer Rioux, Kelli Burt, Cathy Schmidt, coach Francie Kraker-Goodridge

Front Row: Melissa Thompson, Patty Shanahan, Kim Bruce, Metsy Schneider, Carol Lam, Bonnie McDonald

UM Men's Cross Country Team. 1993

Top Row: Nick Watson, Kevin Sullivan, Ian Forsyth, Theo Molla

Middle Row: David Barnett, Matt Schroeder, Ryan Burt, Kenneth Kozloff, Michael Mahler, Don MacDonald

Front Row: head coach Ron Warhurst, Kris Eggle, Jay Schemanske, Mark Kwiatkowski, Jon Aubuchon

Not pictured: Jim Finlayson, Scott MacDonald, Andy Hayes, Robert Lee, Shawn MacKay, Daniel Shea, Chad Tibbetts

Traffic offenders and community service sentencing: an overview. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

The Reformed Presbyterian and covenanter.

Editors: 1863-73, Thomas Sproull (1863-66, with J.M. Willson; 1868-73, with J.W. Sproull); 1874-95, J.W. Sproull and D.B. Willson.

Loran-C signal analysis : final report /

"Report no. CG-D-4-80."

Homelessness : programs and the people they serve : findings of the National Survey of Homeless Assistance Providers and Clients : summary /

"December 1999."

The Fifty-fifth Regiment of the Massachusetts Volunteer Infantry, colord [sic], June 1863-September 1865 /

"Third edition, revized, with additions and in simplified spelling, February, 1919."

California apples situation and outlook, 1949 /

Mode of access: Internet.

Walnut harvesting : methods, equipment, and costs /

Mode of access: Internet.

Uncle William, the man who was shif'less,

Mode of access: Internet.