HIV-1 Tat protein mimicry of chemokines

The HIV-1 Tat protein is a potent chemoattractant for monocytes. We observed that Tat shows conserved amino acids corresponding to critical sequences of the chemokines, a family of molecules known for their potent ability to attract monocytes. Synthetic Tat and a peptide (CysL24–51) encompassing the “chemokine-like” region of Tat induced a rapid and transient Ca2+ influx in monocytes and macrophages, analogous to β-chemokines. Both monocyte migration and Ca2+ mobilization were pertussis toxin sensitive and cholera toxin insensitive. Cross-desensitization studies indicated that Tat shares receptors with MCP-1, MCP-3, and eotaxin. Tat was able to displace binding of β-chemokines from the β-chemokine receptors CCR2 and CCR3, but not CCR1, CCR4, and CCR5. Direct receptor binding experiments with the CysL24–51 peptide confirmed binding to cells transfected with CCR2 and CCR3. HIV-1 Tat appears to mimic β-chemokine features, which may serve to locally recruit chemokine receptor-expressing monocytes/macrophages toward HIV producing cells and facilitate activation and infection.

Collagenase-3 Induction in Rat Lung Fibroblasts Requires the Combined Effects of Tumor Necrosis Factor-α and 12-Lipoxygenase Metabolites: A Model of Macrophage-induced, Fibroblast-driven Extracellular Matrix Remodeling during Inflammatory Lung Injury

The mechanisms responsible for the induction of matrix-degrading proteases during lung injury are ill defined. Macrophage-derived mediators are believed to play a role in regulating synthesis and turnover of extracellular matrix at sites of inflammation. We find a localized increase in the expression of the rat interstitial collagenase (MMP-13; collagenase-3) gene from fibroblastic cells directly adjacent to macrophages within silicotic rat lung granulomas. Conditioned medium from macrophages isolated from silicotic rat lungs was found to induce rat lung fibroblast interstitial collagenase gene expression. Conditioned medium from primary rat lung macrophages or J774 monocytic cells activated by particulates in vitro also induced interstitial collagenase gene expression. Tumor necrosis factor-α (TNF-α) alone did not induce interstitial collagenase expression in rat lung fibroblasts but did in rat skin fibroblasts, revealing tissue specificity in the regulation of this gene. The activity of the conditioned medium was found to be dependent on the combined effects of TNF-α and 12-lipoxygenase-derived arachidonic acid metabolites. The fibroblast response to this conditioned medium was dependent on de novo protein synthesis and involved the induction of nuclear activator protein-1 activity. These data reveal a novel requirement for macrophage-derived 12-lipoxygenase metabolites in lung fibroblast MMP induction and provide a mechanism for the induction of resident cell MMP gene expression during inflammatory lung processes.

1992 Men's Gymnastics Team

Back Row: Christopher Burkhardt, John Besancon, Cory Huttenga, Rich Dopp, Doug McCreery, Geoff Bidwell, Christian Boniforti, Raul Molina, Paul Mariani

Middle Row: Bob Darden-head coach, Royce Toni, Ben Verrall, Matt Marsich, Seth Rubin, Michael Mott, Jorge Camacho, Brian Winkler, asst. Coach Mike Milidonis

Front Row: David Nader, Scott Harris, Josh Miner, Glenn Hill, Jim Round, Ruben Ceballos, trainer Frank Velasquez

1993 Men's Gymnastics Team

Back Row: Mat Burleson, Evan Feldman, Bob Young, Chris Onuska, Brad Terris, Geoff Bidwell, Kris Klinger, Jason Taft

Middle Row: head coach Bob Darden, Chris Burkhardt, Rich Dopp, Chris Bonifiorti, Raul Molina, John Besancon, Coney Huttenga, Paul Mariani, Brian Winkler, mngr. Stacey Shingte, asst. coach Mike Milidonis

Front Row: Royce Toni, Ben Verrall, Seth Rubin, Josh Miners, Matt Marsich, Jorge Camacho, Mike Mott, trainer Jon Fetter

1994 Men's Gymnastics Team

Fourth Row (left to right): Jerrod Farrand, Lawrence Neukom, Flavio Martins, Brad Terris, Jason MacDonald, Paul Bischoff.

Third Row: Jason Taft, Kris Klinger, Evan Feldman, Bob Young, Chris Onuska.

Second Row: Head coach Bob Darden, trainer Tammy Ogeltree, Brian Winkler, Paul Mariani, Christian Boniforti, Raul Molina, Rich Dopp, Cory Huttenga, manager Stacey Shingle, assistant coach Mike Milidonis.

First Row: Matt Marsich, Mike Mott, Ben Verrall, Jorge Camacho, Josh Miner, Royce Toni, Seth Rubin.

Ginnasio di esperimento per i giovani compositori di musica : proposto dal maestro Giacomo Servadio e approvato dall'impresa già costituita.

Mode of access: Internet.

Le sorelline, novelle /

Mode of access: Internet.

Metrical geometry, contact transformations and nuclear physics.

Cover title.

Ignatii de Asso de libris quibusdam hispanorum rarioribus disquisitio.

Mode of access: Internet.

Delle censure dell'abate Antonio Rosmini-Serbati contro la dottrina religiosa di G.D. Romagnosi : saggio /

Mode of access: Internet.

Conversaciones de Lauriso Tragiense, pastor arcade, sobre los vicios y defectos del Teatro moderno y el modo de corregirlos y enmendarlos /

Mode of access: Internet.

Oratio de praestantia, et vtilitate librorum SS. D. N. Benedicti XIV ... De beatificatione seruorum dei, et beatorum canonizatione ... /

Mode of access: Internet.

Storia del processo politico di F.D. Guerrazzi ed altri imputati di perduellione. Corredata di documenti.

Mode of access: Internet.

Alessandro Berthier, principe di Neuchâtel e di Wagram ...

"Estratto dal Fanfulla della domenica, 11 marzo 1900. n.10."