Immune response in human melanoma after transfer of an allogeneic class I major histocompatibility complex gene with DNA–liposome complexes

Analysis of the antitumor immune response after gene transfer of a foreign major histocompatibility complex class I protein, HLA-B7, was performed. Ten HLA-B7-negative patients with stage IV melanoma were treated in an effort to stimulate local tumor immunity. Plasmid DNA was detected within treated tumor nodules, and RNA encoding recombinant HLA-B7 or HLA-B7 protein was demonstrated in 9 of 10 patients. T cell migration into treated lesions was observed and tumor-infiltrating lymphocyte reactivity was enhanced in six of seven and two of two patients analyzed, respectively. In contrast, the frequency of cytotoxic T lymphocyte against autologous tumor in circulating peripheral blood lymphocytes was not altered significantly, suggesting that peripheral blood lymphocyte reactivity is not indicative of local tumor responsiveness. Local inhibition of tumor growth was detected after gene transfer in two patients, one of whom showed a partial remission. This patient subsequently received treatment with tumor-infiltrating lymphocytes derived from gene-modified tumor, with a complete regression of residual disease. Thus, gene transfer with DNA–liposome complexes encoding an allogeneic major histocompatibility complex protein stimulated local antitumor immune responses that facilitated the generation of effector cells for immunotherapy of cancer.

A captain of the Gordons service experiences 1900-1909 /

Mode of access: Internet.

Suite de l'Histoire universelle /

Mode of access: Internet.

Defense against toxin weapons /

Mode of access: Internet.

Bogue genealogy; descendants of John Bogue of East Haddam, Conn., and wife, Rebecca Walkley; also the North Carolina Bogues and miscellaneous Bogue records; ancestors of James Hubbard Bogue and wife, Polly Adelaide Phillips their royal lines,

Includes index.

A genealogical record of Thomas Bascom, and his descendants /

Mode of access: Internet.

Johnsonian gleanings.

Bibliographical footnotes.

Louis Hébert : premier colon canadien et sa famille.

Mode of access: Internet.

Historical sketches and reminiscences of an octogenarian.

Sketches of Washington County, Virginia, including an account of the Preston family.

Memoir of William Madison Peyton, of Roanoke, together with some of his speeches in the House of delegates of Virginia, and his letters in reference to secession and the threatened civil war in the United States, etc., etc.

Appendixes: A. Abridged genealogy, or pedigree of the ancient noble family of Peyton: p. [311]-336; Isleham Hall, the priory and church, co. Cambridge, England: p. 337-354.--B. Memoranda of the Preston family, by O. Brown: p. [355]-374.--C. Abridged pedigree of the Lewis family: p. 375-379.--D. Extract from the Washington pedigree ... by John Washington: p. [380]-383.

Life and letters of Horace Bushnell ...

"The genealogy of Horace Bushnell": p. [569]-590.

Genealogies of the Lewis and kindred families,

Mode of access: Internet.

The Buckners of Virginia and the allied families of Strother and Ashby,

Contains also the Lewis, Washington, Stith, Thruston, Dickinson, Thornton and Claiborne families.