Monoubiquitination and Activity of the Paracaspase MALT1 Requires Glutamate 549 in the Dimerization Interface.

The mucosa-associated lymphoid tissue protein-1 (MALT1, also known as paracaspase) is a protease whose activity is essential for the activation of lymphocytes and the growth of cells derived from human diffuse large B-cell lymphomas of the activated B-cell subtype (ABC DLBCL). Crystallographic approaches have shown that MALT1 can form dimers via its protease domain, but why dimerization is relevant for the biological activity of MALT1 remains largely unknown. Using a molecular modeling approach, we predicted Glu 549 (E549) to be localized within the MALT1 dimer interface and thus potentially relevant. Experimental mutation of this residue into alanine (E549A) led to a complete impairment of MALT1 proteolytic activity. This correlated with an impaired capacity of the mutant to form dimers of the protease domain in vitro, and a reduced capacity to promote NF-κB activation and transcription of the growth-promoting cytokine interleukin-2 in antigen receptor-stimulated lymphocytes. Moreover, this mutant could not rescue the growth of ABC DLBCL cell lines upon MALT1 silencing. Interestingly, the MALT1 mutant E549A was unable to undergo monoubiquitination, which we identified previously as a critical step in MALT1 activation. Collectively, these findings suggest a model in which E549 at the dimerization interface is required for the formation of the enzymatically active, monoubiquitinated form of MALT1.

El documental multimèdia interactiu: una proposta de model d'anàlisi

El propòsit d‘aquest treball de recerca és l‘estudi del procés actual de convergència entre el gènere documental tradicional i el gènere documental interactiu en línia. Pretenem examinar com les noves tecnologies digitals transformen les lògiques de creació i de producció del documental audiovisual convencional. Volem presentar un primer estat de la qüestió sobre les línies de confluència entre els dos àmbits, argumentar una proposta de definició i de caracterització del nou gènere interactiu i avançar unes propostes de model d‘anàlisi del documental multimèdia interactiu, que serà objecte de fonamentació i d‘aplicació en la futura tesi doctoral

A double layer model of the gas bubble/water interface

Zeta potential is a physico-chemical parameter of particular importance to describe sorption of contaminants at the surface of gas bubbles. Nevertheless, the interpretation of electrophoretic mobilities of gas bubbles is complex. This is due to the specific behavior of the gas at interface and to the excess of electrical charge at interface, which is responsible for surface conductivity. We developed a surface complexation model based on the presence of negative surface sites because the balance of accepting and donating hydrogen bonds is broken at interface. By considering protons adsorbed on these sites followed by a diffuse layer, the electrical potential at the head-end of the diffuse layer is computed and considered to be equal to the zeta potential. The predicted zeta potential values are in very good agreement with the experimental data of H-2 bubbles for a broad range of pH and NaCl concentrations. This implies that the shear plane is located at the head-end of the diffuse layer, contradicting the assumption of the presence of a stagnant diffuse layer at the gas/water interface. Our model also successfully predicts the surface tension of air bubbles in a KCl solution. (c) 2012 Elsevier Inc. All rights reserved.

Aplicativo multimídia em plataforma móvel para o ensino da mensuração da pressão venosa central

O objetivo do estudo foi desenvolver e avaliar um aplicativo multimídia em plataforma móvel para o ensino da Mensuração da Pressão Venosa Central (PVC). A pesquisa foi desenvolvida em três fases (Levantamento das necessidades; Metodologia de desenvolvimento do aplicativo multimídia e Avaliação do aplicativo multimídia). A multimídia foi o método escolhido por favorecer um ambiente motivador e dinâmico, integrar imagens e textos num aplicativo disponível para celulares, constituindo-se um meio móvel e autônomo de aprendizagem. Os resultados permitem demonstrar a viabilidade do desenvolvimento da ferramenta para subsidiar a prática pedagógica e abrem perspectivas para acreditar que, na educação em Enfermagem, a tecnologia disponível pode descortinar novos modos de aprender significativamente.

Adolescent support networks in a health care context: the interface between health, family and education

AbstractOBJECTIVEAnalyze adolescents' perceptions about support networks and their health needs.METHODAnalytical and interpretive study using focus groups conducted in municipal state schools in Fortaleza, in the State of Ceará during the first semester of 2012. The sample comprised 36 male and female adolescents aged between 13 and 16 years attending the ninth grade of the second phase of elementary school.RESULTSThematic analysis revealed that the health care support network and interaction between health professionals, education professionals and family members was insufficient, constituting a lack of an integrated network to enable and provide support for health promotion.CONCLUSIONCoordination between education, health and family services has the potential to act as a support network to help meet adolescents' healthcare needs and demands.

Desenvolvimento de Aplicações Web e Multimédia: Desenvolvimento de uma Website

Este relatório descreve o estágio realizado pela autora, no âmbito da licenciatura em Tecnologias de Informação e Comunicação (TIC), da Universidade Pública de Cabo Verde (Uni-CV), realizado na empresa Tera C&D, que decorreu entre 2 Março a 31 Julho de 2009. O referido é o fruto de um projecto, que consistiu no desenvolvimento de um Website dinâmico, com o suporte de uma Base de Dados. Durante o período de estágio, a autora integrada numa equipa composta por quatro elementos, que actuam na área de Gestão de Sistemas Informáticos, Redes de Computadores, Desenvolvimento Web e Multimédia e Programação. Este trabalho foi desenvolvido sob o desígnio de trabalho colaborativo, em que a autora teve o apoio de várias pessoas. No decorrer deste período do estágio, em que foi desenvolvido o projecto, foram executadas um conjunto de actividades desde a análise dos documentos até a criação do site efectivamente. Paralelamente a essas actividades, decorreu uma formação, cujo grande objectivo foi orientar os formandos na execução das actividades e adquirir conhecimentos sobre Novas Tecnologias de Desenvolvimento Web. Considerando os objectivos referidos, o relatório ficará composto por uma parte introdutória, na qual se fará o enquadramento e também serão especificadas, as motivações associadas à escolha do tema. Seguidamente far-se-á a caracterização da empresa acolhedora, focando os aspectos tais como: a actividade principal da empresa, os objectivos os princípios e valores que conduzema mesma. Após fazer a caracterização da empresa acolhedora, far-se-á uma descrição sucinta das actividades realizadas no estágio. O desenvolvimento continuará com o relato de sobre a preparação do ambiente de desenvolvimento, para demonstrar detalhadamente a preparação deste ambiente, antes do inicio do projecto.Tecnologias e Ferramentas utilizadas será o ponto seguinte, no qual vão ser abordadas as diversas tecnologias e ferramentas utilizadas no desenvolvimento do referido projecto. O desenvolvimento segue-se com o tópico “Processo de Desenvolvimento Website”. Neste ponto será descrito pormenorizadamente as diferentes fases do processo de desenvolvimento de um Website, descrevendo com detalhes, o que significa cada fase e as actividades desenvolvidas em cada uma delas. Após descrever o Processo de Desenvolvimento, falar-se-á do projecto desenvolvido propriamente dito, expondo imagens que ilustram cada fase ou actividade realizada, caso for possível. Na parte conclusiva, falar-se-á da importância do desenvolvimento deste trabalho para a autora, do sentimento, das habilidades e competências adquiridas ou aperfeiçoadas.

The State of the Immigrant Body and the Body of the State: Negotiations at the Interface

Using an ethnographic analysis of the social interfaces between state agents and Cape Verdean students in Portugal, observed through participant observation in medical appointments, social work, immigration services and legal support to immigrants, this article aims to examine disciplinary state practices and the negotiations and power struggles that take place. The ethnographic cases discussed demonstrate how the idea of a fair and neutral state is simultaneously reproduced and denied in practice, thus elucidating the state as a symbol of union of an effective disunity. The ethnographic examples also indicate other dimensions of state practice, besides micro-disciplinary powers, which create room for flexibility and adaptation. And it is in this sense that ethnographies of interfaces between state and citizen offer a more relative perspective of excessively systematic interpretations of governmentality, illustrating how the effects of contradictory state practices are as unpredictable as human action itself.

The rate-limiting step for glucose transport into the hypothalamus is across the blood-hypothalamus interface.

Specialized glucosensing neurons are present in the hypothalamus, some of which neighbor the median eminence, where the blood-brain barrier has been reported leaky. A leaky blood-brain barrier implies high tissue glucose levels and obviates a role for endothelial glucose transporters in the control of hypothalamic glucose concentration, important in understanding the mechanisms of glucose sensing We therefore addressed the question of blood-brain barrier integrity at the hypothalamus for glucose transport by examining the brain tissue-to-plasma glucose ratio in the hypothalamus relative to other brain regions. We also examined glycogenolysis in hypothalamus because its occurrence is unlikely in the potential absence of a hypothalamus-blood interface. Across all regions the concentration of glucose was comparable at a given plasma glucose concentration and was a near linear function of plasma glucose. At steady-state, hypothalamic glucose concentration was similar to the extracellular hypothalamic glucose concentration reported by others. Hypothalamic glycogen fell at a rate of approximately 1.5 micromol/g/h and remained present in substantial amounts. We conclude for the hypothalamus, a putative primary site of brain glucose sensing that: the rate-limiting step for glucose transport into brain cells is at the blood-hypothalamus interface, and that glycogenolysis is consistent with a substantial blood -to- intracellular glucose concentration gradient.

Sistemas de Detecção de Intrusões Análise de intrusões na rede multimédia da Unipiaget

Os sistemas computacionais ligados através da rede precisam, cada vez mais, de medidas e mecanismos de protecção que os permitem realizar as suas tarefas de acordo com os objectivos traçados pelos indivíduos ou organizações que os utilizam. Neste sentido, os Intrusion Detection System (IDS) ou Sistemas de Detecção de Intrusão desempenham um papel preponderante na monitorização contínua de actividades de um sistema pretendendo, essencialmente, garantir a autenticidade, confidencialidade, integridade e, sobretudo, a disponibilidade dos seus recursos. O presente trabalho apresenta um estudo sobre os Sistemas de Detecção de Intrusões, apresentando ainda um caso de estudo sobre a Rede Multimédia da Universidade Jean Piaget de Cabo Verde, estudo esse que incide sobre uma ferramenta Open Source, o Snort

Biosphere/lithosphere interface : microbially-mediated CaCO3 precipitation in hypersaline and freshwater environments

Abstract: Microbial mats very efficiently cycle elements, such as C, 0, N, S and H, which makes them key players of redox processes at the biosphere-lithosphere interface. They are characterized by high metabolic activities and high turnover rates (production and consumption) of biomass, which mainly consists of cell material and of extracellular organic matter (EOM). The EOM forms a matrix, embedding the microbial cells and fulfilling various functions within the microbial mat, including: mat attachment to surfaces; creation of micro-domains within the mat; physical stabilization under hy- drodynamic stress and the protection of the cells in multiple other stress conditions. EOM mainly consists of polysaccharides, amino acids, and a variety of chemical func-tional groups {e.g., -C00H, - SH -OH). These groups strongly bind cations such as Ca2+ and Mg2+ and thus exert a strong control on carbonate mineral formation within the microbial mat. A feedback mechanism between community metabolisms, their prod¬ucts, and the surrounding physicochemical microenvironment thus influences the de¬gree of carbonate saturation favoring either carbonate precipitation or dissolution. We investigated the driving forces and mechanisms of microbialite formation in the Sari ne River, FR, Switzerland, the hypersaline lake, Big Pond, Bahamas and in labo¬ratory experiments. The two fundamentally different natural systems allowed us to compare the geochemical conditions and microbial metabolisms, necessary for car¬bonate formation in microbial mats. Although carbonates are oversaturated in both environments, precipitation does not occur on physicochemical substrates (i.e. out¬side the microbial mats). In the Sarine a high crystal nucleation threshold exceeds the carbonate saturation, despite the high carbonate alkalinity in the water column. Cyanobacterial photosynthesis strongly locally enhances the carbonate alkalinity, whereas the EOM attract and immobilize calcium, which increases the saturation state and finally leads to carbonate precipitation within the EOM (in this case the cyanobacterial sheath) as nucleation template. In Big Pond, the presence of calcium- chelating anions (i.e. sulfate) and EOM, as well as the presence of magnesium, lowers the calcium activity in the water column and mat, and thus inhibits carbonate pre¬cipitation. Coupled with other heterotrophic metabolisms, sulfate reduction uses the EOM as carbon source, degrading it. The resulting EOM consumption creates alkalin¬ity, releases calcium and consumes sulfate in mat-micro domains, which leads to the formation of carbonate layers at the top of the microbial mat. Résumé: Interface biosphère/lithosphère: médiation microbienne de la précipitation de CaC03 dans des environnements en eaux douces et hypersalines Les tapis microbiens engendrent une circulation très efficace des éléments, tels que C, 0, N, S et H, ce qui en fait des acteurs clé pour les processus d'oxydoréduction à l'inter¬face biosphère-lithosphère. Ils sont caractérisés par des taux élevés d'activité méta¬bolique, ainsi que par la production et la consommation de biomasse, principalement constituée de cellules microbiennes et de matière organique extracellulaire (MOE). Dans un tapis microbien, les cellules microbiennes sont enveloppées par une matrice de MOE qui a différentes fonctions dont l'attachement du tapis aux surfaces, la créa¬tion de micro-domaines dans le tapis, la stabilisation physique en situation de stress hydrodynamique, et la protection des cellules dans de multiples autres conditions de stress. La MOE se compose principalement de polysaccharides, d'acides aminés, et d'une variété de groupes fonctionnels chimiques (par exemple, COOH, -SH et -OH). Ces groupes se lient fortement aux cations, tels que Ca2+ et Mg2+, et exercent ainsi un contrôle fort sur la formation de CaC03 dans le tapis microbien. Un mécanisme de rétroaction, entre les métabolismes de la communauté microbienne, leurs produits, et le microenvironnement physico-chimique, influence le degré de saturation de car¬bonate, favorisant soit leur précipitation, soit leur dissolution. Nous avons étudié le moteur et les mécanismes de minéralisation dans des tapis de la Sarine, FR, Suisse et du lac hypersalin, Big Pond, aux Bahamas, ainsi que durant des expériences en laboratoire. Les deux systèmes naturels, fondamentalement dif¬férents, nous ont permis de comparer les conditions géochimiques et les métabolis¬mes nécessaires à la formation des carbonates dans des tapis microbiens. Bien que les carbonates soient sursaturés dans les deux environnements, la précipitation ne se produit pas sur des substrats physico-chimiques (en dehors du tapis microbien). Dans la Sarine, malgré un taux d'alcalinité élevé, les valeurs de seuil pour la nucléa- tion de carbonates sont plus hautes que la saturation du carbonate. La photosynthèse cyanobactérienne augmente localement l'alcalinité, alors que la MOE attire et immo¬bilise le calcium, ce qui augmente l'état de saturation et conduit finalement à la pré¬cipitation des carbonates, en utilisant la MOE comme substrat de nucléation. À Big Pond, la présence de chélateurs de calcium, notamment les anions (p.ex. le sulfate) et la MOE, ainsi que la présence de magnésium, réduit l'activité du calcium et inhibe en conséquence la précipitation des carbonates. Couplée avec d'autres métabolismes hétérotrophes, la réduction des sulfates utilise la MOE comme source de carbone, en la dégradant. Cette consommation de MOE crée l'alcalinité, consomme des sulfates et libère du calcium dans des micro-domaines, conduisant à la formation de couches de carbonates dans le haut du tapis microbien.

Multimédia e sua aplicação no ensino secundário em Cabo Verde

O presente trabalho monográfico intitulado “Multimédia e a sua aplicação no ensino secundário em Cabo Verde”, tem como propósito analisar o uso da multimédia no ensino secundário em Cabo Verde com particularidade especial em relação à ilha do Fogo. A obtenção dos dados analisados processou-se mediante a aplicação de questionários para professores e alunos e de entrevistas realizadas junto dos Directores das escolas secundárias. Para qualquer pessoa que não tenha conhecimento em termos de multimédia ao ler este trabalho ficará com algumas ideias do que é multimédia, dos elementos que a constituem, o modo de divulgação dos conteúdos, as vantagens e as desvantagens e entre outros inerentes à mesma.