Alcohol and alcohol safety. A curriculum manual for senior high level. Volume II of II. A teacher's activities guide.

National Highway Traffic Safety Administration, Washington, D.C.

Achieving a high level of smoothness in concrete pavements without sacrificing long-term performance /

"HRDI-11/10-05(2M)E"--P. [4] of cover.

Alcohol and alcohol safety. A curriculum manual for junior high level. Volume II of II. A teacher's activities guide.

National Highway Traffic Safety Administration, Washington, D.C.

Alcohol and alcohol safety. A curriculum manual for senior high level. Volume I of II.

National Highway Traffic Safety Administration, Washington, D.C.

Alcohol and alcohol safety. A curriculum manual for junior high level. Volume I of II.

National Highway Traffic Safety Administration, Washington, D.C.

The coder : a program module for code generation in high-level language compilers /

Originally presented as the author's thesis (M.S.), University of Illinois at Urbana-Champaign.

Long-term management of liquid high-level radioactive wastes stored at the Western New York Nuclear Service Center, West Valley : final Environmental Impact Statement.

"June 1982."

The Third International Seabed High-Level Waste Disposal Assessment Workshop, Albuquerque, New Mexico, February 6-7, 1978 : a report to the NEA Radioactive Waste Management Committee /

Mode of access: Internet.

The perpetual care of high-level radioactive liquid wastes in New York State; a Technological and cost study,

This work performed under contract C-16348 for the New York State Office of Atomic Development.

The Illinois Spent Nuclear Fuel and High-Level Waste Inspection and Escort Program /

"5/99"--Colophon.

High-level food consumption in the United States /

"December 1945."

The comparative study of high-level manpower in developing societies : a tentative framework /

Mode of access: Internet.

Rabbit CYP4B1 engineered for high-level expression in Escherichia coli: ligand stabilization and processing of the N-terminus and heme prosthetic group

Modifications at the N-terminus of the rabbit CYP4B1 gene resulted in expression levels in Escherichia coli of up to 660 nmol/L. Solubilization of the enzyme from bacterial membranes led to substantial conversion to cytochrome P420 unless alpha-naphthoflavone was added as a stabilizing ligand. Mass spectrometry analysis and Edman sequencing of purified enzyme preparations revealed differential N-terminal post-translational processing of the various constructs expressed. Notably, bacterial expression of CYP4B1 produced a holoenzyme with >98.5% of its heme prosthetic group covalently linked to the protein backbone. The near fully covalently linked hernoproteins exhibited similar rates and regioselectivities of lauric acid hydroxylation to that observed previously for the partially heme processed enzyme expressed in insect cells. These studies shed new light on the consequences of covalent heme processing in CYP4B1 and provide a facile system for future mechanistic and structural studies with the enzyme. (C) 2003 Elsevier Science (USA). All rights reserved.