Lithium reduces Gsk3b mRNA levels: implications for Alzheimer Disease

There is evidence of increased systemic expression of active GSK3B in Alzheimer`s disease patients, which apparently is associated with the formation of senile plaques and neurofibrillary tangles. Due to its central role in the pathogenesis of AD, GSK3B is currently a promising target of the pharmaceutical industry. Whilst trials with specific GSK inhibitors in AD are under way, major attention has been focused on the neuroprotective effects of lithium. Whereas the direct and indirect inhibitory effects of lithium over GSK3 activity have been documented by several groups, its effects over Gsk3 transcription have not yet been addressed. We used quantitative PCR to evaluate the transcriptional regulation of Gsk3a and Gsk3b in lithium-treated primary cultures of rat cortical and hippocampal neurons. We found a significant and dose-dependent reduction in the expression of Gsk3b, which was specific to hippocampal cells. This same effect was further confirmed in vivo by measuring Gsk3 expression in different brain regions and in peripheral leukocytes of adult rats treated with lithium. Our studies show that LiCl can modulate Gsk3b transcription in vitro and in vivo. This observation suggest new regulatory effects of lithium over Gsk3b, contributing to the better understanding of its mechanisms of action, offering a new and complementary explanation for Gsk3b modulation and reinforcing its potential for the inhibition of key pathological pathways in Alzheimer`s disease.

Mechanism of Muscle Vibrations During Stimulated and Voluntary Isometric Contractions of Mammalian Skeletal Muscle

When a muscle contracts it produces vibrations. The origin of these vibrations is not known in detail. The purpose of this study was to determine the mechanism associated with muscle vibrations. Mechanisms which have been proposed in the literature were described as theories (cross-bridge cycling, vibrating string and unfused motor unit theories). Specific predictions were derived from each theory, and tested in three conceptually different studies. In the first study, the influence of recruitment strategies of motor units (MUs) on the vibromyographic (VMG) signal was studied in the in-situ cat soleus using electrical stimulation of the soleus nerve. VMG signals increased with increasing recruitment and decreased with increasing firing rates of MUs. Similar results were obtained for the human rectus femoris (RF) muscle using percutaneous electrical stimulation of the femoral nerve. The influence of MU activation on muscle vibrations was studied in RF by analyzing VMG signals at different percentages (0-100%) of the maximal voluntary contraction (MVC). In our second study, we tested the effects of changing the material properties of the in-situ cat soleus (through muscle length changes) on the VMG signal. The magnitude of the VMG signal was higher for intermediate muscle lengths compared to the longest and the shortest muscle lengths. The decreased magnitude of the VMG signal at the longest and at the shortest muscle lengths was associated with increased passive stiffness and with decreased force transients during unfused contractions, respectively. In the third study, the effect of fatigue on muscle vibrations was studied in human RF and vastus lateralis (VL) musc1es during isometric voluntary contractions at a leveI of 70% MVC. A decrease in the VMG signal magnitude was observed in RF (presumably due to derecruitment of MUs) and an increase in VL (probably related to the enhancement of physiological tremor, which may have occurred predorninantly in a mediolateral direction) with fatigue. The unfused MU theory, which is based on the idea that force transients produced by MUs during unfused tetanic contraction is the mechanism for muscle vibrations, was supported by the results obtained in the above three studies.

Encontro com Eduardo Escorel

Encontro com o cineasta Eduardo Escorel, coordenador acad��mico da P��s-Gradua����o em Cinema Document��rio da FGV. Apresenta����o sobre o surgimento e desenvolvimento do que hoje se conhece como cinema document��rio e sobre as no����es de 'documento' e de 'verdade' que s��o a ele associados. [Parte 1: Introdu����o, Sinos da Profundeza, Werner Herzog, Encena����o no Document��rio]. [Parte 2: Origem do curso de Cinema Document��rio da FGV]. [Parte 3: Matuszewsk, Imagem como Documento]. [Parte 4: Matuszewsk, Edward S. Curtis]. [Parte 5: Edward S. Curtis, Na Terra dos Ca��adores de Cabe��a]. [Parte 6: Flaherty, Moana]. [Parte 7: Grierson, Drifters]. [Parte 8: Fury, Fritz Lang, Credibilidade do Document��rio]. [Parte 9: A Imagem como Prova, Carta da Sib��ria, Chris Marker]. [Parte 10: Shoah, Claude Lanzmann]. [Parte 11: Shoah, Claude Lanzmann]. [Parte 12: Sinos da Profundeza, Werner Herzog, Informa����es sobre a P��s-Gradua����o em Cinema Document��rio da FGV]. [Parte 13: Informa����es sobre a P��s-Gradua����o em Cinema Document��rio da FGV].

Se inicia ora����o com pronome cl��tico? Atitudes lingu��sticas, na escola, em rela����o aos padr��es brasileiros de coloca����o pronominal

Focusing on the empirical assessment issue, proposed by Language change theory (cf. cf. WEINREICH; LABOV; HERZOG, 2006; LABOV, [1972] 2008), this research assists to clarifying Portuguese teacher s attitudes in Natal- RN, regarding (a) to proclisis in three specific contexts: in the beginning of a simple/compound sentence (V1), after the subjects (SV), and proclisis after the secondary verb in complex verbal structures (V1V2); (b) to students who use such patterns in usage. Specific contexts were gathered thanks to their representing of the standard variety, as many studies have proven (Martins, 2012; Schei, 2003; Biazzoli, 2010, 2012). The research aims at: (i) verifying by means of a classroom assessment test, whether Portuguese teachers correct proclisis on referred contexts; (ii) identifying, via attitudinal tests what actions teachers take regarding to the usage of standards above mentioned, as well as students as users of those. Twenty Portuguese teachers, picked at random out of different of public schools in Natal-RN, responded to a classroom assessment test in addition to other two attitudinal ones. Results achieved point to a recurring high proclisis correction index of 50% in simple/compound sentences, even though such variety has been implemented to pronominal usage standards in Brazilian Portuguese. This setting of usage was generally assessed negatively, having no commonality between this assessment and the neutral one used by students. Unlike previous setting, the proclisis after subject did not receive any correction of the twenty teachers, what proves coherence with the positive evaluation both the varieties and the students attained. As for the second verb of complex verbal structures, proclisis correction went negative on presenting single results, despite their proximity, with correction indexes of 20% (infinite structures), 10% (present progressive structures) and 25% (participle structures). The assessment on these contexts of proclisis ranged between positive and neutral, also valid for the one students utilized. It means that proclisis in the beginning of simple/compound sentences are yet seemingly spotted in writing school scenario, much likely due to the negative evaluation, opposite to students . Later to subjects and earlier to secondary verbs in structures, proclisis appears to be acknowledged in writing school scenarios, which reflects on teachers assessment as compared to students who use proclisis in these contexts; being in general either positive or neutral

A implementa����o do voc�� em cartas pessoais norte-riograndenses do s��culo XX

Considering the theoretical and methodological presuppositions of Variationist Sociolinguistics (cf. WEINREICH; LABOV; HERZOG, 2006; LABOV, [1972] 2008), in this dissertation, we describe and analyze the process of variation/change involving the personal pronouns tu and voc��, and its extension in the pronominal paradigm in Brazilian Portuguese (BP), in three sets of personal letters written by people from Rio Grande do Norte (RN) along the 20th century. The discursive universe of those letters is news from the cities in which the informers lived and the themes from their everyday life (trade, jobs, trips, family and politics). Part of the analyzed letters integrate the written by hand minimum corpus of the Projeto de Hist��ria do Portugu��s Brasileiro no Rio Grande do Norte (PHPB-RN). We are based on previous studies about the pronominal system in BP Menon (1995), Faraco (1996), Lopes e Machado (2005), Rumeu (2008), Lopes (2009), Lopes, Rumeu e Marcotulio (2011), Lopes e Marcotulio (2011) e Martins e Moura (2012) , which register the form voc�� replaces tu from the end of the first half of 20th century and attest the following situation: while (a) the imperative verbal forms, (b) the explicit subjects and (c) prepositional complement pronouns are favorable contexts for voc��, the (d) non imperative verbal forms (with null subject), (e) the non prepositional complement pronoun and (f) the possessive pronoun are contexts of resistance of tu. The results got in this dissertation confirm, partially, the statements defended by the previous studies regarding the favorable contexts for the implementation of voc�� in BP: (i) there are, in the letters from the first two decades of 20th century (1916 to 1925), high frequency of the usage of the form voc�� (98%); (ii) in the personal letters of RN especially in the love letters, in which there are higher recurrence of intimate subjects the discursive universe proved to be itself very relevant in the determination/conditions of the forms of tu; (iii) the unique feminine informer of our sample uses, almost categorically, the forms of tu in letters of the period from 1946 to 1972; (iv) the letters corresponding to the period from 1992 to 1994 present a significant usage of the forms associated to the innovating voc��, letting appear the change is already implemented in the system of BP and there are, in that set of letters, strong evidences that make us state the pronominal forms of non prepositional complement (accusative/ dative) related to tu are also implemented in a system with an almost categorical usage of voc��

A express��o do condicionado contrafactual em constru����es se p, ent��o q no portugu��s brasileiro

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico

A comparison study of periapical repair in dogs' teeth using RoekoSeal and AH plus root canal sealers: A histopathological evaluation

The objective of the study was to evaluate the biocompatibility of RoekoSeal sealer Roeko (Dental Products, Langenau, Germany) with the periapical tissues of dogs and compare it with AH Plus sealer (Dentsply/De Trey, Konstanz, Germany). The pulps of 32 root canals were removed, the apical cementum layer perforated, the biomechanical preparation performed, and the root canals filled by lateral condensation technique. Ninety days after the surgery, the animals were euthanized, the bone with teeth removed, and the samples prepared for histopathological analysis. In group 1 (RoekoSeal Automix), deposition of mineralized tissue was observed, with complete newly mineralized apical formed tissue in 43.8% and partial sealing in 56.2%. In group 2 (AH Plus), in 12.5% there was complete newly mineralized apical formed tissue, in 75% the sealing was partial, and in 12.5% there was no sealing (p < 0.05). There were no differences between the groups in relation to the inflammatory infiltrate; thickness of the periodontal ligament; and the resorption of dentin, cementum or bone (p > 0.05).

Varia����o sazonal de clorofilas em folhas de Tabebuia avellanedae e Anadenanthera colubrina

The experiment had the aim of quantifying seasonal chlorophyll variation on leaves of Anadenanthera colubrina and Tabebuia avellanedae. The experiment used a randomized block design established as a 2 x 3 factorial (two species and three sampling seasons) with 5 replications. The concentrations of chlorophyll in leaves were performed at the end of winter, late spring, and summer. The content of chlorophyll a changed only for A. colubrina, bringing up from spring (117.52 mu g mL(-1)) to summer (151.13 mu g mL(-1)). For the two species, the proportion of chlorophyll b was higher between periods at the end of spring (average of 29.31 mu g mL(-1)) to the end of summer (average of 74.96 mu g mL(-1)) with close relationship with the decrease in global solar irradiance. The chlorophyll a/b relationship increased in late spring due to reduction of chlorophyll b content. A. colubrina showed greater adaptation to the studied environment.

Alternative measures of potential economic growth in Latin America

Includes bibliography

A concord��ncia verbal na l��ngua falada na regi��o noroeste do Estado de S��o Paulo

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Concord��ncia verbal e varia����o: um estudo descritivo-comparativo do portugu��s brasileiro e do portugu��s europeu

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Relembrando a Est��ria de Kaspar Hauser

Com base na teoria piagetiana, enfocando as rela����es pensamento e linguagem, esse artigo tenta tra��ar um paralelo entre Kaspar Hauser ��� personagem do filme ���Jeder f��r sich und Gott gegen alle��� dirigido pelo cineasta alem��o W. Herzog ��� e L. F., garoto cego de nascen��a, com 10 anos, que cresceu ���abandonado���, entregue a seus pr��prios recursos e que s�� come��ou a freq��entar escola aos 8 anos de idade.

Varia����o e gramaticaliza����o no uso de preposi����es em contextos de verbos de movimento no portugu��s brasileiro

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)