Human herpesvirus type 6 and type 1 infection increases susceptibility to nonmelanoma skin tumors

In order to investigate herpesvirus (HHV) role in the susceptibility to skin cancer, we compared HHV6 and HHV1 incidence in DNA samples extracted from 120 lesions and 41 normal skin tissues. HHV6 (31.7%) and HHV1 (23.8%) were detected more frequently in skin cancer than in control individuals (14.6 and 5%, respectively) (P=0.0391 and P=0.00094, respectively). The risk of presenting basal cell carcinomas (BCC) was more than 3 times higher for HHV-6 infected patients (OR=3.182; 95% CI: 1.125-8.997). The risk for HHV-1 infected individuals of presenting BCC and squamous cell carcinomas was increased 8 and 6 times, respectively (OR=8.125; 95% CI: 1.735-38.043 and OR=6.290; 95% CI: 1.283-30.856, respectively). (c) 2004 Elsevier B.V.. All rights reserved.

Peso de abate de machos não-castrados para produção do bovino jovem. 1. Desempenho em confinamento e custos de produção

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Peso de abate de machos não-castrados para produção do bovino jovem. 2. Peso, Idade e caracteristicas da carcaça

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Kaposi's sarcoma-associated herpesvirus infection and Kaposi's sarcoma in Brazil

Kaposi's sarcoma (KS) became a critical health issue with the emergence of acquired immunodeficiency syndrome (AIDS) in the 1980s. Four clinical-epidemiological forms of KS have been described: classical KS, endemic KS,iatrogenic KS, and AIDS-associated KS. In 1994, Kaposi's sarcoma-associated herpesvirus (KSHV) or human herpesvirus type 8 was identified by Chang and colleagues, and has been detected worldwide at frequencies ranging from 80 to 100%. The aim of the present study was to evaluate the frequency of KSHV infection in KS lesions from HIV-positive and HIV-negative patients in Brazil, as well as to review the current knowledge about KS transmission and detection. For these purposes, DNA from 51 cases of KS was assessed by PCR: 20 (39.2%) cases of classical KS, 29 (56.9%) of AIDS-associated KS and 2 (3.9%) of iatrogenic KS. Most patients were males (7.5:1, M/F), and mean age was 47.9 years (SD = ± 18.7 years). As expected, HIV-positive KS patients were younger than patients with classical KS. On the other hand, patients with AIDS-associated KS have early lesions (patch and plaque) compared to classical KS patients (predominantly nodular lesions). This is assumed to be the result of the early diagnose of KS in the HIV-positive setting. KSHV infection was detected by PCR in almost all cases (48/51; 94.1%), irrespectively of the clinical-epidemiological form of KS. These results show that KSHV is associated with all forms of KS in Brazilian patients, a fact that supports the role of this virus in KS pathogenesis. © 2006 Brazilian Journal of Medical and Biological Research.

Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino

The objective of this study was to evaluate the effect of beta-lactoglobulin polymorphism, breed and seasonality on physico-chemical characteristics and stability of bovine milk. Six Holstein-Zebu (H-Z) and five Holstein dairy herds were selected, of which 660 Holstein and 293 crossbred Holstein-Zebu milk and blood samples were collected, adding up 953 samples, distributed in two collecting periods at rainy and two at dry seasons. Each milk sample was analyzed for titratable acidity, pH, freezing point and milk ethanol stability, at the following ethanol concentrations: 70, 76, 80 and 84GL. Individual blood samples were submitted to polymerase chain reaction (PCR) for the determination of beta-lactoglobulin polymorphism. No effect of beta-lactoglobulin polymorphism was observed in physico-chemical characteristics of milk, in both studied breed. Breed effect for Holstein and H-Z, respectively, was observed on titratable acidity (16.16 and 17.07°D, while effect of seasonality (for rainy and dry seasons, respectively) was also observed on freezing point (-0.5411 and -0.5376°H). Effects of breed and seasonality on milk stability were observed (Holstein-Zebu milk was less stable on dry season), however, no effect of beta-lactoglobulin on milk stability was observed.

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen 1 mimics Epstein-Barr virus EBNA1 immune evasion through central repeat domain effects on protein processing

Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV8]) and Epstein-Barr virus (EBV/HHV4) are distantly related gammaherpesviruses causing tumors in humans. KSHV latency-associated nuclear antigen 1 (LANA1) is functionally similar to the EBV nuclear antigen-1 (EBNA1) protein expressed during viral latency, although they have no amino acid similarities. EBNA1 escapes cytotoxic lymphocyte (CTL) antigen processing by inhibiting its own proteosomal degradation and retarding its own synthesis to reduce defective ribosomal product processing. We show here that the LANA1 QED-rich central repeat (CR) region, particularly the CR2CR3 subdomain, also retards LANA1 synthesis and markedly enhances LANA1 stability in vitro and in vivo. LANA1 isoforms have half-lives greater than 24 h, and fusion of the LANA1 CR2CR3 domain to a destabilized heterologous protein markedly decreases protein turnover. Unlike EBNA1, the LANA1 CR2CR3 subdomain retards translation regardless of whether it is fused to the 5′ or 3′ end of a heterologous gene construct. Manipulation of sequence order, orientation, and composition of the CR2 and CR3 subdomains suggests that specific peptide sequences rather than RNA structures are responsible for synthesis retardation. Although mechanistic differences exist between LANA1 and EBNA1, the primary structures of both proteins have evolved to minimize provoking CTL immune responses. Simple strategies to eliminate these viral inhibitory regions may markedly improve vaccine effectiveness by maximizing CTL responses. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Estudo da perda da tensao do enxerto de tendão calcâneo bovino

Lesion of the anterior cruciate ligament (ACL) in the knee is a problem that affects animals as well as humans and may evolve with joint instability that is often symptomatic and incapacitating. The main option for ligament substitution is the autologous tendon graft. The graft undergoes tensioning during ACL reconstructive surgery to reestablish the normal laxity of the ACL- deficient knee. Although graft tensioning plays a fundamental role in postoperative clinical evolution, ideal tensioning levels have not been established in the literature. Therefore, graft elongation that may occur over time is still one of the main reasons for ligament reconstruction failure. In this study, ten bovine calcaneus tendons underwent two successive assays of physiological tensioning for a maximum deformation of 2.5% of the initial tendon length, maintained for 600s with force (N) values recorded at zero time (initial), 300s and 600s. At the end of the first assay, the tendon returned to its initial length and was maintained at rest for 300s and then the next tensioning assay was initiated, repeating the previous procedure. Statistical analysis revealed that tendon elongation is more pronounced during the initial 300s, reflecting a more accentuated decline in the tension values of the graft. Therefore, 300s after tensioning and fixing the graft, the surgeon can assess with more precision if the tensioning level was adequate.

Esterco bovino para o desenvolvimento inicial de plantas provenientes de quatro matrizes de Corymbia citriodora

This work aimed to evaluate the effect of cattle manure levels in the phase of implementation Corymbia citriodora (Hook.) K. D. Hill & L. A. S. Johnson (Eucalyptus citriodora) seedlings in field, produced from seeds collected from four mother trees. The experiment was carried out in green house, in pots filled with 5 dm 3 of Oxisol. Five cattle manure levels were applied 0, 10, 20, 30, and 40 t ha -1 (0, 25, 50, 75, and 100 g pot -1). All treatments received lime levels which were calculated to increase the base saturation degree to 50%. The soil fertility was evaluated after 30 days of soil incubation with manure and lime. The seeds were collected from mother trees called 2, 8, 20 and 29. Sowing was performed directly in rigid plastic containers of 50 cm 3 and seedlings were transplanted when they were around 17 cm height. The experimental design was entirely randomized, in a factorial 5 × 4 (five doses of cattle manure and seeds of four mother trees) scheme and four repetitions. Each parcel was a pot with two plants. At 90 days the height, foliar area, stalk diameter, shoot and root dry matter. The plants responded positively to application of manure, but differently for each evaluated growth characteristic linearly or quadratically. The manure levels had linear effects on growth characteristics and dry matter production of the plants from mother trees 2 and 20 moreover these plants require more cattle manure levels than ones from mother trees 8 and 29. The cattle manure promoted the best development of plants from mother trees 8 and 29, in relationship with dry matter production of shoot components was approximately 27 t ha-1, equivalent to 67.5 g pot.

produção de mudas de maracujazeiro amarelo submetidas à salinidade em solo com biofertilizante bovino

The experiment was conducted in a greenhouse from May 1 to July 31, 2008, in Areia county, Paraiba State, PB, Brazil, in order to evaluate the effects of irrigation water salinity on initial growth of the passionfruit seedlings in non-saline substrate with and without bovine biofertilizer. The treatments were distributed in a completely randomized design, with three replications and twelve plants per plots, in a factorial arrangement 5 × 2 × 2, corresponding the former to the levels of salinity in the irrigation water: 0.5; 1.0; 2.0; 3.0 and 4.0 dS m-1, in soils with and without bovine biofertilizer applied at two moments (25 and 65 days after seedling emergence). The growth of the seedlings and the soil electrical conductivity were evaluated at the end of the experiment. The biofertilizer was diluted in a low saline water at a 1:1 ratio and was applied once two days before sowing, corresponding to 10% of the substrates volume. The increase in water salinity inhibited the growth in height of plants, leaf area and root length, but always to a lesser extent in the treatments with bovine biofertilizer. The increase in electrical conductivity of the irrigation water elevated the soil salinity, independently of the addition of biofertilizer.

Atividade antimicrobiana in vitro de extrato de Punica granatum L. sobre staphylococcus aureus isolado em leite bovino

Bovine mastitis is considered important disease causing major economic losses in dairy herds. Antimicrobial drug can promote resistance, chemical residues in food and environmental contamination. The purpose of this study was to evaluate the antibacterial activity in vitro of pomegranate extract on Staphylococcus aureus isolated from bovine milk, evaluate its antioxidant activity, and quantify levels of total phenols and fravonoides of the different extracts used. Aqueous extracts were used in nature and dry, from the peel of the fruit (EAC) and leaves (EAF). Additionally, it was evaluated the antioxidant activity (AA%), total phenols and flavonoids. Milk samples were inoculated, incubated, and the colonies were characterized as Staphylococcus aureus were adjusted to 1.0x106 UFC/mL in the 6 standard of the MacFarland scale. The sensitivity of the microbial isolates were determined in quintuplicate, in disk diffusion test. The minimum inhibitory concentration was determined by visible inhibition zone greater than 15mm. The results were evaluated by ANOVA, Tukey 5%, using the program SISVAR 5.3 - DEX/UFLA. Results implied that the aqueous extract from the bark of dried fruit was capable of inhibiting bacterial growth at concentrations of 3%. The other treatments only showed this activity, from the concentrations of 15%, 20% and 30% for dry EAF, in nature EAC and in nature EAF, respectively. Regarding the action antioxidant of dry EAC, was not correlated with total phenols and flavonoids. Probably other alkaloids substances present in the extract studied, may have been responsible for this activity. It is concluded that extracts of Punica granatum L., especially those obtained by the shell of the fruit, showed inhibitory activity against S. aureus, indicating your use potential for the control of bovine mastitis.

Crescimento inicial e consumo hídrico de nim submetido ao estresse salino e biofertilizante bovino

An experiment was carried out during the period of January to July/2010, in municipality of Areia, Paraiba State, Brazil, in order to evaluate effects of the irrigation with saline water, bovine biofertilizer and drainage of the soil on water consumption and growth of neem seedlings. The experimental design was in randomized blocks using factorial 5×2×2, referring to five levels of saline water (0.5; 1.5; 3.0; 4.5; 6.0 dS nr-1) in soil without and with bovine biofertilizer and in pots without and with drainage. In plants the water consumption, growth in height, stem diameter, number of leaves, dry matter of roots, aerial part and total dry mass were evaluated and in soil the electrical conductivity of saturation extract - EC was determined. The bovine biofertilizer, after dilution in non saline water (0.49 dS nr-1) e no chlorinated water in 1:1 ratio was applied once two days before sowing, equivalent to 10% of substrate volume. Irrigation was applied daily with each water type applying volume sufficient to maintain the soil with water content at level of field capacity. From results the increase in salinity of water inhibited the water consumption by plants independently of the soil with or without bovine biofertilizer. The salinity of water in soil with and without bovine biofertilizer also reduced the growth of neem plants but with more pronounced effect in the treatments without application of organic fertilizer to soil in liquid form.

Comportamento do noni à salinidade da água de irrigação em solo com biofertilizante bovino

Salt excess in soil and water used for irrigation can cause significant loss of production and growth in cultivated plants. Among some options for reduction of negative effects of salts to plants in cultivated areas, fermented bio fertilizer has been used to grow vegetables and fruit tree irrigated with saline water. The study aimed at evaluating the behavior of the noni plant to salinity of the irrigation water in substrate with and with no bio fertilizer. Treatments were arranged in a randomized block design with four replications, using a 5 × 2 factorial arrangement. Five levels of electrical conductivity of irrigation water (0.5, 1.5, 3.0, 4.5, 6.0 dS m-1) were used in substrates with and with no bio fertilizer. Parameters were evaluated as follows: plant height, stem diameter, number of leaves, leaf area, shoot dry matter and water consumption. All evaluated variables were negatively affected by the increase in salt concentration of the irrigation water, but always with less intense effects in treatments with bio fertilizer.The bio fertilizer does not eliminate, but mitigates the negative effects of salts in noni plants.

Estudo comparativo entre peritônio bovino e biomembrana de látex natural na substituição de fragmento da parede da bexiga em coelhos (Oryctolagus cuniculus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência das proteínas morfogenéticas ósseas adsorvidas à hidroxiapatita e aglutinantes do colágeno bovino na consolidação da fratura do rádio em coelhos

Pós-graduação em Medicina Veterinária - FMVZ

Avaliação comparativa das técnicas de ELISA indireto, antígeno acidificado tamponado, 2-mercaptoetanol com soroaglutinação lenta e fixação de complemento para o diagnóstico da brucelose em rebanho bovino não vacinado, rebanho vacinado com dose padrão e rebanho vacinado com dose reduzida da amostra 19 de Brucella abortus

Pós-graduação em Medicina Veterinária - FMVZ