646 resultados para Girolamo Muzio
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Coaut. tomado de pr
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Vieta en port
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Vieta en port
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Vieta en port
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Vieta en port
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Marca tip. en port
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Brefeldin A, a fungal metabolite that inhibits membrane transport, induces the mono(ADP-ribosyl)ation of two cytosolic proteins of 38 and 50 kDa as judged by SDS/PAGE. The 38-kDa substrate has been previously identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report that the 50-kDa BFA-induced ADP-ribosylated substrate (BARS-50) has native forms of 170 and 130 kDa, as determined by gel filtration of rat brain cytosol, indicating that BARS-50 might exist as a multimeric complex. BARS-50 can bind GTP, as indicated by blot-overlay studies with [alpha-32P]GTP and by photoaffinity labeling with guanosine 5'-[gamma-32P] [beta,gamma-(4-azidoanilido)]triphosphate. Moreover, ADP-ribosylation of BARS-50 was completely inhibited by the beta gamma subunit complex of G proteins, while the ADP-ribosylation of GAPDH was unmodified, indicating that this effect was due to an interaction of the beta gamma complex with BARS-50, rather than with the ADP-ribosylating enzyme. Two-dimensional gel electrophoresis and immunoblot analysis shows that BARS-50 is a group of closely related proteins that appear to be different from all the known GTP-binding proteins.
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Inscripcin en ngulo derecho: "2"
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Mode of access: Internet.
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Reprint: Rassegna Nazionale (Firenze : Ufficio della Rassegna Nazionale), anno 12, fasc. 478.
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bk.1. 1-7 -- bk.2. 8-12.
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The plates are engraved by A. Rivelanti and Girolamo Carattoni.
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Title vignette.
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Includes errata, V1r, V3v.
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[superscript pi]A a2-a4 b A-F G H-2O [2P] 2Q 2R 2S 2T-2X 2Y 2Z-3A 3B 3C-6T [6V-6Z] [dagger-2 daggers] chi-2chi.