High resolution ultrastructural mapping of total calcium: electron spectroscopic imaging/electron energy loss spectroscopy analysis of a physically/chemically processed nerve-muscle preparation.

We report on a procedure for tissue preparation that combines thoroughly controlled physical and chemical treatments: quick-freezing and freeze-drying followed by fixation with OsO4 vapors and embedding by direct resin infiltration. Specimens of frog cutaneous pectoris muscle thus prepared were analyzed for total calcium using electron spectroscopic imaging/electron energy loss spectroscopy (ESI/EELS) approach. The preservation of the ultrastructure was excellent, with positive K/Na ratios revealed in the fibers by x-ray microanalysis. Clear, high-resolution EELS/ESI calcium signals were recorded from the lumen of terminal cisternae of the sarcoplasmic reticulum but not from longitudinal cisternae, as expected from previous studies carried out with different techniques. In many mitochondria, calcium was below detection whereas in others it was appreciable although at variable level. Within the motor nerve terminals, synaptic vesicles as well as some cisternae of the smooth endoplasmic reticulum yielded positive signals at variance with mitochondria, that were most often below detection. Taken as a whole, the present study reveals the potential of our experimental approach to map with high spatial resolution the total calcium within individual intracellular organelles identified by their established ultrastructure, but only where the element is present at high levels.

Sedimentology and age on profile Lz1023

A 270 cm long sediment sequence was recovered with a piston corer from east lobe Bonney, Taylor Valley, Antarctica, and characterized according to its sedimentological, mineralogical, and geochemical properties. It is the first record of such length recovered from east lobe Bonney. The sediment core is mainly composed of halite crystals of different sizes, water, and a relatively low and stable proportion of clastic particles. Although the sediment surface was probably disturbed by the coring process and absence or low contents of organic material or carbonates hampers the establishment of a robust chronology by radiocarbon dating, the core probably contains at least several hundred years of information about the history of the lake and the Bonney basin. Variations in halite crystal sizes and amount as well as variations in the composition of clastic material can be related to past lake level changes and evaporation cycles.