Rational design of a global inhibitor of the virulence response in Staphylococcus aureus, based in part on localization of the site of inhibition to the receptor-histidine kinase, AgrC

Two-component signaling systems involving receptor-histidine kinases are ubiquitous in bacteria and have been found in yeast and plants. These systems provide the major means by which bacteria communicate with each other and the outside world. Remarkably, very little is known concerning the extracellular ligands that presumably bind to receptor-histidine kinases to initiate signaling. The two-component agr signaling circuit in Staphylococcus aureus is one system where the ligands are known in chemical detail, thus opening the door for detailed structure–activity relationship studies. These ligands are short (8- to 9-aa) peptides containing a thiolactone structure, in which the α-carboxyl group of the C-terminal amino acid is linked to the sulfhydryl group of a cysteine, which is always the fifth amino acid from the C terminus of the peptide. One unique aspect of the agr system is that peptides that activate virulence expression in one group of S. aureus strains also inhibit virulence expression in other groups of S. aureus strains. Herein, it is demonstrated by switching the receptor-histidine kinase, AgrC, between strains of different agr specificity types, that intragroup activation and intergroup inhibition are both mediated by the same group-specific receptors. These results have facilitated the development of a global inhibitor of virulence in S. aureus, which consists of a truncated version of one of the naturally occurring thiolactone peptides.

Kyōto-shi, Japan, ca. 1732 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan de Miaco résidence de l'empereur ecclésiastique : copié d'après l'original japonnois ... qui se trouve dans le Cabinet du Chevalier Hans Sloane = Platte grondt van de stad Miaco, zetel van den geestelyken erf-keyzer van Japan : na een origineele japansche kaart ... onder den Ridder Hans Sloane berustende door J. G. Scheuchser. It was published by chez R. & I. Ottens in ca. 1732. Scale [ca. 1:14,000]. Covers Kyōto-shi, Japan. Map in French and Dutch.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Tokyo Universal Transverse Mercator (UTM) Zone 53N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, streets, mountains, selected buildings, and more. Relief shown pictorially.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Levensschets van sulthan Ibrahiem, vorst van Eirakh: uit het Arabisch in het Maleisch vertaald door eenen Maleischen schriftgeleerden

en uit het Maleisch in het Nederduitsch overgebracht door Philippus Pieter Roorda van Eijsinga.

The tarnished golden door : civil rights issues in immigration.

Item 288-A.

Album studiosorum Academiae Groninganae, uitgegeven door het Historisch genootschap te Groningen.

Mode of access: Internet.

Statistical evaluation of the effectiveness of Federal Motor Vehicle Safety Standard 214: Side Door Strength. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Catalogus van schilderijen teekeningen en beelden in het Stedelijk Museum : bijeengebracht door de vereeniging tot het vormen van een openbare verzameling van hedendaagsche kunst te Amsterdam.

Mode of access: Internet.

Door locking systems study. Phase 1. Final report.

National Highway Safety Bureau, Washington, D.C.

Performance requirements for doors, door retaining devices and adjacent structures.

National Highway Safety Bureau, Washington, D.C.

Evaluation methodologies for four federal motor vehicle safety standards. FMVSS 214: side door strength. FMVSS 301: fuel system integrity. FMVSS 215: exterior protection. FMVSS 208: occupant crash protection. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Final design and implementation plan for evaluating the effectiveness of FMVSS 214: side door strength. Tasks #4 & #5 report.

National Highway Traffic Safety Administration, Washington, D.C.

Side door latch/hinge assembly evaluation. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Cost evaluation for nine Federal Motor Vehicle Safety Standards - Task IX: FMVSS 214, side door strength, identification and cost evaluation of design and manufacturing changes. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

A preliminary evaluation of seat back locks for two-door passenger cars with folding front seatbacks. Technical report.

Mode of access: Internet.