Perfil lipídico da membrana plasmática e alterações morfofuncionais de esperamatozóides de garanhões resistentes e sensíveis à criopreservação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Erva mate minimiza as alterações do perfil lipídico promovidas por elevado consumo de sacarose

The yerba mate (Ilex paraguariensis) is composed of bioactive that interfere with lipid metabolism. The objective was to evaluate if daily consumption of mate tea (MT) change the lipid deposits and dyslipidemia caused by excessive consumption of sucrose. Thirty male Wistar rats (40 days old) were divided into four groups: Group C - free access to commercial chow and deionized water; S - free access to commercial food, water and sucrose solution 30% (w/v) in water; MTS and deionized - free access to commercial feed solution, water, 30% sucrose (w/v) and treated with daily infusion of MT (soluble mate Leão Júnior®) via orogastric tube at a dose of 100 mg/kg/mc for 8 weeks. After the trial period the lipid profile was evaluated by the following parameters: a) direct weighing of the retroperitoneal (RP) and epididymal (EPI) adipose tissues; b) determination of plasma total cholesterol, triglycerides and HDL cholesterol concentration. The MT promoted the reduction of 1.4 times in both tissues RP and EPI in MTS group compared to group S. Treatment with MT decreased 2.7 times triglyceride in the MTS group compared with the group S. The sucrose consumption did not alter the plasma cholesterol concentration, however the consumption of MT significantly reduced total cholesterol circulating. The HDL cholesterol concentration, in the MTS group, showed higher concentration than in group S (1.3 times). MT prevents in young male rats the increase of lipid deposits and dyslipidemia caused by excessive consumption of sucrose.

Efeito do treinamento aquático no perfil lipídico e na hipertrofia ventricular esquerda de camundongos hiperlipidêmicos

This study assessed the effects of Water training on the lipid profile and left ventricular structures in hyperlipidemic mice. Twenty-eight male LDLr-/- mice were randomly separated into 4 groups: sedentary, fed a standard diet (C); exercising, fed a standard diet (C+TRE); sedentary, fed a hyperlipidic diet (C+HL); and exercising, fed a hyperlipidic diet (E+HL). The exercising mice trained daily for 60 minutes during 60 days. After 48 hours of the end of the training period and 12 hours of rest fasting the animals were underwent euthanasia and the blood was collected for measuring the plasma levels of triglycerides, total cholesterol and its fractions (LDL, HDL, VLDL). The heart was removed and the left ventricle was weighed fresh to calculate the ratio left-ventricle weight (mg)/body weight (g). The results showed that the training was more effective in improving lipid plasma levels when combined with a balanced diet, thereby confirming that it is essential to associate physical exercise and diet. The training protocol resulted in eccentric left ventricular hypertrophy in the standard-diet group and decreased interstitial collagen deposition in the myocardium of the high-fat-diet animals, which may indicate an improved diastolic function with consequent improvement in the systolic function. It was concluded that regular moderate aerobic exercise induce beneficial and prophylactic adaptations to heart which promoted a better health condition and prevention to diseases.

Acúmulo de cádmio, crômio e níquel e isolamento de micro-organismos potenciais para biorremediação em área agrícola

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relação amônio e nitrato, mitigação da toxicidade amoniacal com silício e curva de acúmulo de nutrientes em mudas de maracujazeiro

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Efeitos de reguladores do metabolismo lipídico no desenvolvimento in vitro de embriões bovinos e na sobrevivência à vitrificação

Pós-graduação em Medicina Veterinária - FCAV

Teor e acúmulo de nutrientes em grãos de feijão comum em semeadura direta, sob déficit hídrico

O feijão comum possui notória importância socioeconômica, constitui uma das mais importantes fontes proteicas da dieta brasileira e juntamente com o arroz, proporciona uma dieta mais vantajosa e equilibrada em termos de aminoácidos. O objetivo desse trabalho foi avaliar o efeito das combinações de diferentes reposições hídricas nas fases vegetativa - I e reprodutiva - II, durante o ciclo do feijoeiro IAC Alvorada, e comparar o teor nutricional dos grãos. Foi utilizado o delineamento em blocos casualizados, com quatro repetições em fatorial 4 x 4, sendo quatro níveis de déficit hídrico 100, 80, 60 e 40% da evapotranspiração da cultura (ETc) e os mesmos níveis foram repetidos em duas fases de desenvolvimento da cultura do feijão (fases I e II). Cada parcela possuía as dimensões de 4 m x 1,8 m, totalizando 7,2 m2 de 0,45 m. Inicialmente irrigou-se pelo sistema aspersão convencional em um período de 60 minutos diariamente, posteriormente o sistema adotado foi por gotejamento, conforme cada tratamento de restrição hídrica. Avaliaram-se os teores nutricionais de macro (N, P, K, Ca, Mg e S) e micronutrientes (B, Cu, Fe, Mn e Fe). A interação entre as lâminas nas fases I e II influenciou nos teores de micronutrientes Fe e S em grãos de feijão. Houve aumento dos teores de Fe e diminuição dos teores de Cu, Mn e B com a deficiência hídrica em uma das fases. Os macro e micronutrientes mais extraídos foram N, P, K, Fe, B e Mn. O N e o P são os nutrientes exportados em maior quantidade pelos grãos.

Acúmulo de micronutrientes em beterraba submetida a diferentes manejos de fertirrigação e niveis de salinidade

Knowledge of the amount of nutrients accumulated in the plant provides important information that can assist in the fertilization of crops program. To study the salt effect caused by high amounts of fertilizers applied by fertigation, an experiment was conducted in a greenhouse at the FCA/UNESP, Botucatu, SP. The experiment lasted 90 days (01/11/11 to 29/01/12) constituting five levels of electrical conductivity (1.0, 3.0, 6.0, 9.0, and 12.0 dS m-1), fertigation two management (M1: traditional management, M2: management by controlling the ionic concentration of the soil solution) and two varieties of beets (Early Wonder and Itapuã) blocks with 4 repeats forming a 5x2x2 factorial. From the dry weight of shoots and roots was determined the levels of micronutrients (B, Cu, Fe, Mn, Zn mg kg-1), and by multiplying the dry matter accumulation was determined in these plant . The accumulation of micronutrients in different parts of the plant followed the order: Fe> Mn> Zn> B> Cu for the different management fertigation studied. The Cu, Fe, Mn element present responses to increased soil salinity leading to greater absorption.

Acúmulo de nutrientes e resposta da alface à adubação fosfatada

Nutrient absorption and response of lettuce to phosphorus fertilization. The objective of this study was to evaluate the response of lettuce (cultivar Verônica) to different levels of phosphorus fertilization. The experiment was conducted from 25/09/2003 (sowing) to 03/12/2003 (harvesting) at the Fazenda Experimental São Manuel, UNESP/FCA, Botucatu/SP, under protective structures. The experiment was conducted in a randomized block design, with five treatments (0, 200, 400, 600 and 800kg.ha-1 of P2 O5, in the form of triple superphosphate) and five replications. Plants were grown in 13L plastic pots containing Latossolo Vermelho Distrófico Típico. A quadratic response was observed for the fresh mass of the plant shoots and leaf area, with maximum equivalent levels of 733 and 756kg.ha-1 of P2 O5 , respectively, as well as for potassium, calcium, magnesium, sulfur, boron, copper, iron, manganese and zinc accumulation. Linear increases were observed with the treatments of P2 O5 for dry mass of the shoots, leaf number, plant height and nitrogen and phosphorus accumulation.

Acúmulo de macronutrientes e produtividade de genótipos de cártamo (Carthamus tinctorius L.) em função da adubação nitrogenada no sistema plantio direto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Perfil lipídico da membrana citoplasmática de espermatozoides in natura e criopreservados de jumentos e cavalos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Investigação da expressão de genes do metabolismo lipídico em pacientes com Diabetes Mellitus tipo 2, Dislipidemia e Doença Periodontal Crônica

Pós-graduação em Odontologia - FOAR

Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial

The action of man has led, over the years, major impacts on the environment, especially in aquatic ecosystems, leading to an impairment of water quality, considered one of the essential factors for the maintenance of vital functions and consequently the life of the planet. Among the activities considered a risk for the environment are linked to pollution from many sources and even entire watersheds, whether by industrial waste, sewage, or for substances used in agriculture, such as pesticides, herbicides and fertilizers. The (2,4-D) 2,4- dichlorophenoxyacetic acid is used worldwide, and the fact that its genotoxicity is proven by several studies and by its long persistence in soil, which enables the leaching and percolation of compounds affecting water bodies, toxicity studies are relevant and justifiable. Thus, this study aimed to evaluate the toxicity of 2,4-D by examining the liver of the fish Oreochromis niloticus exposed to different dilutions. Portions of liver were collected and fixed for histological and histochemical techniques to detect total proteins, polysaccharides and lipids. lipids. Treatment with 2,4-D herbicide apparently did not alter the lipid profiles, the accumulation of polysaccharides, and the presence of total proteins. The 2.5 and 5.0% were lethal to fish. These mortalities are probably of high toxic and cytotoxic potential of 2,4-D herbicide results. Several histopathological changes were found, such as: loss of cytoplasmic integrity, loss of cell limit, nuclear deformation, vacuolated cytoplasm, tissue disorganization and hydropic degeneration. Statistically significant changes were: hydropic degeneration and vacuolated cytoplasm. It is concluded, therefore, that the qualitative morphological analysis is an important method for observing changes in liver toxicology studies. As the O. niloticus species is an efficient biological indicator of water pollution by 2,4-D

Investigação da expressão de genes do metabolismo lipídico em pacientes com Diabetes Mellitus tipo 2, Dislipidemia e Doença Periodontal Crônica

Pós-graduação em Odontologia - FOAR