Identification of Inositol 1,3,4-Trisphosphate 5-Kinase and Inositol 1,3,4,5-Tetrakisphosphate 6-Kinase in Immature Soybean Seeds

In extracts of immature soybean (Glycine max [L.] Merr.) seeds inositol tetrakisphosphate was formed from [3H]inositol 1,3,4-trisphosphate but not from [3H]inositol 1,4,5-trisphosphate. Inositol 1,3,4-trisphosphate kinase was purified to a specific activity of 3.55 min−1 mg−1 by polyethylenimine clarification and anion-exchange chromatography. The partially purified enzyme converted [3H]inositol 1,3,4-trisphosphate to inositol 1,3,4,5-tetrakisphosphate as the major product and inositol 1,3,4,6- and/or 1,2,3,4-tetrakisphosphate as the minor product. Subsequent experiments revealed a separate inositol 1,3,4,5-tetrakisphosphate 6-kinase activity, which could link these enzymes to inositol hexakisphosphate synthesis via the previously reported inositol 1,3,4,5,6-pentakisphosphate 2-kinase. The apparent Km values for inositol 1,3,4-trisphosphate kinase were 200 ± 0 nm for inositol 1,3,4-trisphosphate and 171 ± 4 μm for ATP, and the reaction was not reversible. The kinetics were such that no activity could be detected using unlabeled inositol 1,3,4-trisphosphate and [γ-32P]ATP, which suggested that other kinases may have been observed when less purified fractions were incubated with radiolabeled ATP. Inositol 1,3,4-trisphosphate kinase was nonspecifically inhibited more than 80% by various inositol polyphosphates at a concentration of 100 μm.

Cinq pieces pour le piano, op.6, no.1 and 2, Prelude et nocturne.

Mode of access: Internet.

Employment and pay rolls in state unemployment compensation systems, 1938. Employment security memorandum no. 6. pt. 1-2.

Pt. 1. Introduction and charts. Pt. 2. Tables.

Operator's manual : aviator's night vision imaging system AN/AVS-6(V)1, (NSN 5855-01-138-4749) and AN/AVS-6(V)2, (NSN 5855-01-138-4748).

"5 July 1983."

Two drawings of a house: Sheet no. 02, perspective north-east, 5/2/52; sheet no. 1, plan lover level, 5/2/52 revised 6/28/52

Lance Burghardt bracketed his exposures and provided 3 different files of these items (2 tifs, 1 jpg). Each shares same housescan number, differentiated by suffix 1of3, 2of3, or 3of3....