Reconfigurable IEEE1451-FPGA based weblab infrastructure

Weblabs are spreading their influence in Science and Engineering (S&E) courses providing a way to remotely conduct real experiments. Typically, they are implemented by different architectures and infrastructures supported by Instruments and Modules (I&Ms) able to be remotely controlled and observed. Besides the inexistence of a standard solution for implementing weblabs, their reconfiguration is limited to a setup procedure that enables interconnecting a set of preselected I&Ms into an Experiment Under Test (EUT). Moreover, those I&Ms are not able to be replicated or shared by different weblab infrastructures, since they are usually based on hardware platforms. Thus, to overcome these limitations, this paper proposes a standard solution that uses I&Ms embedded into Field-Programmable Gate Array (FPGAs) devices. It is presented an architecture based on the IEEE1451.0 Std. supported by a FPGA-based weblab infrastructure able to be remotely reconfigured with I&Ms, described through standard Hardware Description Language (HDL) files, using a Reconfiguration Tool (RecTool).

Uma aplicação Web para apoio a laboratórios remotos reconfiguráveis baseados na norma IEEE 1451.0

Nos últimos anos, o processo de ensino e aprendizagem tem sofrido significativas alterações graças ao aparecimento da Internet. Novas ferramentas para apoio ao ensino têm surgido, nas quais se destacam os laboratórios remotos. Atualmente, muitas instituições de ensino disponibilizam laboratórios remotos nos seus cursos, que permitem, a professores e alunos, a realização de experiências reais através da Internet. Estes são implementados por diferentes arquiteturas e infraestruturas, suportados por vários módulos de laboratório acessíveis remotamente (e.g. instrumentos de medição). No entanto, a sua inclusão no ensino é ainda deficitária, devido: i) à falta de meios e competências técnicas das instituições de ensino para os desenvolverem, ii) à dificuldade na partilha dos módulos de laboratório por diferentes infraestruturas e, iii) à reduzida capacidade de os reconfigurar com esses módulos. Para ultrapassar estas limitações, foi idealizado e desenvolvido no âmbito de um trabalho de doutoramento [1] um protótipo, cuja arquitetura é baseada na norma IEEE 1451.0 e na tecnologia de FPGAs. Para além de garantir o desenvolvimento e o acesso de forma normalizada a um laboratório remoto, este protótipo promove ainda a partilha de módulos de laboratório por diferentes infraestruturas. Nesse trabalho explorou-se a capacidade de reconfiguração de FPGAs para embutir na infraestrutura do laboratório vários módulos, todos descritos em ficheiros, utilizando linguagens de descrição de hardware estruturados de acordo com a norma IEEE 1451.0. A definição desses módulos obriga à criação de estruturas de dados binárias (Transducer Electronic Data Sheets, TEDSs), bem como de outros ficheiros que possibilitam a sua interligação com a infraestrutura do laboratório. No entanto, a criação destes ficheiros é bastante complexa, uma vez que exige a realização de vários cálculos e conversões. Tendo em consideração essa mesma complexidade, esta dissertação descreve o desenvolvimento de uma aplicação Web para leitura e escrita dos TEDSs. Para além de um estudo sobre os laboratórios remotos, é efetuada uma descrição da norma IEEE 1451.0, com particular atenção para a sua arquitetura e para a estrutura dos diferentes TEDSs. Com o objetivo de enquadrar a aplicação desenvolvida, efetua-se ainda uma breve apresentação de um protótipo de um laboratório remoto reconfigurável, cuja reconfiguração é apoiada por esta aplicação. Por fim, é descrita a verificação da aplicação Web, de forma a tirar conclusões sobre o seu contributo para a simplificação dessa reconfiguração.

L'émergence d'un principe de justice distributive en droit international économique : analyse de l'évolution du traitement spécial et différencié du GATT à l'OMC

La libéralisation des échanges a fait augmenter les richesses, mais en réalité, elles se sont concentrées dans les pays développés. La question de la distribution plus équitable des richesses s'est rapidement posée. Le système GATT/OMC a joué un rôle décisif dans la libéralisation des échanges et dans l'articulation des rapports entre les pays développés et les pays en développement (PED). L'émergence et l'incarnation juridique dans le système GATT/OMC d'un principe de justice distributive passe par l'évolution du traitement spécial et différencié (TSD). Sous le GATT, le TSD s'est d'abord manifesté par l'article XVIII et la Partie IV du GATT de 1947, la Clause d'habilitation et le Système de préférences de 1971. Le TSD ainsi proposé appartenait essentiellement à la sof law et a échoué dans sa tentative d'intégrer les PED au système SCM. Sous l'OMC, le TSD a changé de paradigme et de mandat. Le TSD est passé d'un outil voué à mettre au développement des PED à un mécanisme employé à aider les PED à mettre en œuvre les nouvelles politiques de libéralisation découlant des accords de l'OMC. Les dispositions TSD seront alors dispersées dans l'ensemble des accords de l'OMC, mais sans jamais transcender la forme «soft law» qui les caractérisait sous le GATT. L'échec de la Conférence de Seattle, en 1999, engendrera le «Programme de Doha pour le développement», en 2001. La Déclaration de Doha était alors perçue comme l'incarnation de la transformation de l'OMC en organisation qui se préoccupe désormais de justice distributive. En observant de près le texte de la Déclaration de Doha et en analysant sa valeur juridique, on ne constate pas de progrès significatifs. Encore une fois, les mesures proposées le sont sous forme de déclarations d'intention et de promesses, voire d'engagement à négocier. Actuellement, le Cycle de Doha tarde à aboutir et tout nous porte à croire que l'avènement de l'OMC n'a pas concrétisé la volonté des PED d'une répartition plus équitable des richesses.

Limited sampling strategies for estimation of cyclosporine exposure in pediatric hematopoietic stem cell transplant recipients : methodological improvement and introduction of sampling time deviation analysis.

Le suivi thérapeutique est recommandé pour l’ajustement de la dose des agents immunosuppresseurs. La pertinence de l’utilisation de la surface sous la courbe (SSC) comme biomarqueur dans l’exercice du suivi thérapeutique de la cyclosporine (CsA) dans la transplantation des cellules souches hématopoïétiques est soutenue par un nombre croissant d’études. Cependant, pour des raisons intrinsèques à la méthode de calcul de la SSC, son utilisation en milieu clinique n’est pas pratique. Les stratégies d’échantillonnage limitées, basées sur des approches de régression (R-LSS) ou des approches Bayésiennes (B-LSS), représentent des alternatives pratiques pour une estimation satisfaisante de la SSC. Cependant, pour une application efficace de ces méthodologies, leur conception doit accommoder la réalité clinique, notamment en requérant un nombre minimal de concentrations échelonnées sur une courte durée d’échantillonnage. De plus, une attention particulière devrait être accordée à assurer leur développement et validation adéquates. Il est aussi important de mentionner que l’irrégularité dans le temps de la collecte des échantillons sanguins peut avoir un impact non-négligeable sur la performance prédictive des R-LSS. Or, à ce jour, cet impact n’a fait l’objet d’aucune étude. Cette thèse de doctorat se penche sur ces problématiques afin de permettre une estimation précise et pratique de la SSC. Ces études ont été effectuées dans le cadre de l’utilisation de la CsA chez des patients pédiatriques ayant subi une greffe de cellules souches hématopoïétiques. D’abord, des approches de régression multiple ainsi que d’analyse pharmacocinétique de population (Pop-PK) ont été utilisées de façon constructive afin de développer et de valider adéquatement des LSS. Ensuite, plusieurs modèles Pop-PK ont été évalués, tout en gardant à l’esprit leur utilisation prévue dans le contexte de l’estimation de la SSC. Aussi, la performance des B-LSS ciblant différentes versions de SSC a également été étudiée. Enfin, l’impact des écarts entre les temps d’échantillonnage sanguins réels et les temps nominaux planifiés, sur la performance de prédiction des R-LSS a été quantifié en utilisant une approche de simulation qui considère des scénarios diversifiés et réalistes représentant des erreurs potentielles dans la cédule des échantillons sanguins. Ainsi, cette étude a d’abord conduit au développement de R-LSS et B-LSS ayant une performance clinique satisfaisante, et qui sont pratiques puisqu’elles impliquent 4 points d’échantillonnage ou moins obtenus dans les 4 heures post-dose. Une fois l’analyse Pop-PK effectuée, un modèle structural à deux compartiments avec un temps de délai a été retenu. Cependant, le modèle final - notamment avec covariables - n’a pas amélioré la performance des B-LSS comparativement aux modèles structuraux (sans covariables). En outre, nous avons démontré que les B-LSS exhibent une meilleure performance pour la SSC dérivée des concentrations simulées qui excluent les erreurs résiduelles, que nous avons nommée « underlying AUC », comparée à la SSC observée qui est directement calculée à partir des concentrations mesurées. Enfin, nos résultats ont prouvé que l’irrégularité des temps de la collecte des échantillons sanguins a un impact important sur la performance prédictive des R-LSS; cet impact est en fonction du nombre des échantillons requis, mais encore davantage en fonction de la durée du processus d’échantillonnage impliqué. Nous avons aussi mis en évidence que les erreurs d’échantillonnage commises aux moments où la concentration change rapidement sont celles qui affectent le plus le pouvoir prédictif des R-LSS. Plus intéressant, nous avons mis en exergue que même si différentes R-LSS peuvent avoir des performances similaires lorsque basées sur des temps nominaux, leurs tolérances aux erreurs des temps d’échantillonnage peuvent largement différer. En fait, une considération adéquate de l'impact de ces erreurs peut conduire à une sélection et une utilisation plus fiables des R-LSS. Par une investigation approfondie de différents aspects sous-jacents aux stratégies d’échantillonnages limités, cette thèse a pu fournir des améliorations méthodologiques notables, et proposer de nouvelles voies pour assurer leur utilisation de façon fiable et informée, tout en favorisant leur adéquation à la pratique clinique.

Influence of compounding and process variables on quality of condams made from natural rubber latex

Condoms are widely accepted as a contraceptive for family planning and population control. It is also accepted as the most effective barrier against sexually transmitted diseases, especially AIDS, the incurable disease. But presence of pinholes and low film strength of condoms make it unsuitable for the purpose. Quality improvement of condoms by reducing the pinhole formation and increasing the film strength is thus an essential requirement for population control as well as for preventing the spread of sexually transmitted diseases. Strict implementation of WHO specification of condoms further increases the rejection percentage. This causes higher rejection loss to condom manufacturers because the defects could be identified only at the final stage of processing. If the influence of various factors which cause these defects is known, manufacturers can take remedial measures to reduce the defectives so that rejection loss can be decreased and quality of condoms increased. In the present study, it was proposed to conduct experiments to improve the quality of condoms by reducing the pinhole rejection percentage and increasing the tensile properties, burst volume, and burst pressure. Ageing property improvement also was an important target among other parameters. Until a cure for AIDS is found, a high quality latex condom is the only effective device in the prevention of the spread of HIV, AIDS and STD's. Hence it is all the more necessary to have high quality condoms.

Comportamiento anticonceptivo en estudiantes universitarios y riesgo de infección con el virus de inmunodeficiencia humana (VIH)

Se examinan los comportamientos anticonceptivos de 225 jóvenes universitarios (22'7% hombres y 77'3% mujeres) con el objetivo de conocer su eficacia para evitar embarazos no deseados, SIDA y otras enfermedades de transmisión sexual (ETS). Se recogió información sobre los diferentes métodos anticonceptivos empleados por los sujetos con su última pareja, sin establecer ningún límite temporal. La mitad de los estudiantes no habían tenido nunca relaciones sexuales completas. La mitad de las conductas heterosexuales de los jóvenes sexualmente activos fueron adecuadas para protegerse frente al SIDA, otras ETS y embarazos no deseados (usar siempre preservativo); un tercio de las conductas únicamente eran útiles para evitar embarazos (píldora anticonceptiva) y las restantes (usar ocasionalmente el preservativo o el coitus interruptus ) les pusieron en riesgo frente a los tres problemas. Además, conforme se eleva la edad o el número de parejas de los estudiantes, es más frecuente el uso de píldoras anticonceptivas y menos probable el empleo de preservativo. Estos resultados sugieren que los estudiantes sexualmente activos están más interesados en la prevención de los embarazos no deseados que en evitar infectarse con el SIDA u otras ETS, por lo que seria conveniente promocionar entre ellos el uso del preservativo como método anticonceptivo, destacando sus ventajas frente a la píldora

Prevención de los comportamientos sexuales de riesgo en los adolescentes: SIDA, otras enfermedades de transmisión sexual y embarazos no deseados

En la actualidad, los adolescentes son uno de los grupos que por sus características conductuales, cognitivas y sociales, se encuentran en mayor riesgo frente al posible contagio con el VIH. Esta amenaza para la salud y el bienestar de los adolescentes, se ha venido a sumar a otros problemas ya existentes en este colectivo: los embarazos no deseados y las enfermedades de transmisión sexual (ETS), que también se derivan de la no utilización de precauciones en las relaciones sexuales. En este trabajo se exponen diversos factores biológicos, psicológicos (conductuales y cognitivos) y sociales que pueden facilitar, dificultar o impedir los comportamientos sexuales de prevención de los adolescentes, y se revisan y valoran algunas de las intervenciones preventivas realizadas. A partir de esta revisión se argumenta sobre la conveniencia de unificar los programas para la prevención simultánea de los tres trastornos, maximizando de esta forma los recursos disponibles

Investigación perfil en competencias y habilidades del empresario bogotano: trabajo de campo

Este documento corresponde al trabajo de campo para la investigación doctoral de Luis Alberto Estrada titulado “Perfil en competencias del empresario bogotano”. El objetivo que el equipo de investigación definió fue concretar las habilidades y competencias que acompañan a los emprendedores y fundadores de empresa en la primera década del siglo XXI. Para ello, se tomó una base de datos de la Cámara de Comercio de Bogotá, la cual contiene la información comercial de las empresas de la ciudad con matrícula mercantil vigente creadas en el periodo de 2000 a 2011 con un mínimo de 5 años de ventas registradas. Mediante el análisis de la información se seleccionó a las cincuenta empresas de mayor crecimiento en ventas, de las cuales ocho fueron encuestadas y entrevistadas junto a sus fundadores. Los hallazgos que se encontraron en este estudio muestran un perfil del grupo de encuestados con respecto a su formación, su relación, su gestión, sus aspiraciones, su compostura, su visión y su trascendencia.

Teleconsultation in sexual and reproductive health for young adults through mobile devices: Experience in Colombia

Background: Sexual risk behaviors associated with poor information on sexuality have contributed to major public health problems in the area of sexual and reproductive health in teenagers and young adults in Colombia. Objective: To measure the perception of changes in sexual and reproductive risk behavior after the use of a teleconsultation service via mobile devices in a sample of young adults. Methods: A before and after observational study was designed, where a mobile application to inquire about sexual and reproductive health was developed. The perception of changes in sexual and reproductive health risk behaviors in a sample of young adults after the use of the application was measured using the validated survey “Family Health International (FHI) – Behavioral Surveillance Survey (BSS) – Survey for Adults between 15 to 40 Years”. Non-probabilistic convenience recruitment was undertaken through the study´s web page. Participants answered the survey online before and after the use of the mobile application for a six month period (intervention). For the inferential analysis, data was divided into three groups (dichotomous data, discrete quantitative data, and ordinal data), to compare the results of the questions between the first and the second survey. For all tests, a confidence interval of 95% was established. For dichotomous data, the Chi-squared test was used. For quantitative data, we used the Student’s t-test, and for ordinal data, the Mann-Whitney-Wilcoxon test. Results: A total of 257 subjects were registered in the study and met the selection criteria. The pre-intervention survey was answered by 232 subjects, and 127 completely answered the post-intervention survey, of which 54.3% did not use the application, leaving an effective population of 58 subjects for analysis. 53% (n=31) were female, and 47% (n=27) were male. The mean age was 21 years, ranging between 18 and 40 years. The differences between the answers on the first and the second survey were not statistically significant. The main risk behaviors identified in the population were homosexual relations, non-use of condoms, sexual relations with non-regular and commercial partners, the use of psychoactive substances, and ignorance about the symptoms of sexually transmitted diseases and HIV transmission. Conclusions: Although there were no differences between the pre- and post-intervention results, the study revealed different risk behaviors among the participating subjects. These findings highlight the importance of promoting educational strategies on this matter and the importance of providing patients with easily accessible tools with reliable health information.

Conhecimentos e atitudes face ao uso de métodos contraceptivos e à prevenção das ist's em jovens

O aumento das infecções sexualmente transmissíveis e da gravidez indesejada, fez com que os jovens fossem considerados um grupo de intervenção prioritário. Esta investigação avaliou os conhecimentos e as atitudes face aos métodos contraceptivos e ISTs em jovens. Administraram-se 436 questionários a 113 rapazes e 323 raparigas, entre os 18 e os 24 anos. Aplicaram-se medidas de conhecimento (CKI ; STD-HBKT ) e de atitudes (CAS ; STD- AS ) acerca dos métodos contraceptivos e das ISTs. Os resultados obtidos demonstram que a maioria é sexualmente activa e utiliza simultaneamente preservativo e pílula. Os rapazes referem mais frequentemente parceiros ocasionais e maior frequência de actividade sexual sob efeito de álcool ou drogas que as raparigas. Relativamente aos conhecimentos dos métodos e das ISTs, encontraram-se diferenças estatisticamente signficativas entre individuos de sexo diferentes: as raparigas apresentam mais conhecimentos e preocupação preventiva face aos risco e os rapazes mais aceitação do risco.

Biomineralisation by earthworms: an investigation into the stability and distribution of amorphous calcium carbonate

Background Many biominerals form from amorphous calcium carbonate (ACC), but this phase is highly unstable when synthesised in its pure form inorganically. Several species of earthworm secrete calcium carbonate granules which contain highly stable ACC. We analysed the milky fluid from which granules form and solid granules for amino acid (by liquid chromatography) and functional group (by Fourier transform infrared (FTIR) spectroscopy) compositions. Granule elemental composition was determined using inductively coupled plasma-optical emission spectroscopy (ICP-OES) and electron microprobe analysis (EMPA). Mass of ACC present in solid granules was quantified using FTIR and compared to granule elemental and amino acid compositions. Bulk analysis of granules was of powdered bulk material. Spatially resolved analysis was of thin sections of granules using synchrotron-based μ-FTIR and EMPA electron microprobe analysis. Results The milky fluid from which granules form is amino acid-rich (≤ 136 ± 3 nmol mg−1 (n = 3; ± std dev) per individual amino acid); the CaCO3 phase present is ACC. Even four years after production, granules contain ACC. No correlation exists between mass of ACC present and granule elemental composition. Granule amino acid concentrations correlate well with ACC content (r ≥ 0.7, p ≤ 0.05) consistent with a role for amino acids (or the proteins they make up) in ACC stabilisation. Intra-granule variation in ACC (RSD = 16%) and amino acid concentration (RSD = 22–35%) was high for granules produced by the same earthworm. Maps of ACC distribution produced using synchrotron-based μ-FTIR mapping of granule thin sections and the relative intensity of the ν2: ν4 peak ratio, cluster analysis and component regression using ACC and calcite standards showed similar spatial distributions of likely ACC-rich and calcite-rich areas. We could not identify organic peaks in the μ-FTIR spectra and thus could not determine whether ACC-rich domains also had relatively high amino acid concentrations. No correlation exists between ACC distribution and elemental concentrations determined by EMPA. Conclusions ACC present in earthworm CaCO3 granules is highly stable. Our results suggest a role for amino acids (or proteins) in this stability. We see no evidence for stabilisation of ACC by incorporation of inorganic components.

Subclinical thyroid dysfunctions are independent risk factors for mortality in a 7.5-year follow-up: the Japanese-Brazilian thyroid study

Objective: The currently available data concerning the influence of subclinical thyroid disease (STD) on morbidity and mortality are conflicting. Our objective was to investigate the relationships between STD and cardiometabolic profile and cardiovascular disease at baseline, as well as with all-cause and cardiovascular mortality in a 7.5-year follow-up. Design: Prospective, observational study. Methods: An overall of 1110 Japanese-Brazilians aged above 30 years, free of thyroid disease, and not taking thyroid medication at baseline were studied. In a cross-sectional analysis, we investigated the prevalence of STD and its relationship with cardiometabolic profile and cardiovascular disease. All-cause and cardiovascular mortality rates were assessed for participants followed for up to 7.5 years. Association between STD and mortality was drawn using multivariate analysis, adjusting for potential confounders. Results: A total of 913 (82.3%) participants had euthyroidism, 99 (8.7%) had subclinical hypothyroidism, and 69 (6.2%) had subclinical hyperthyroidism. At baseline, no association was found between STD and cardiometabolic profile or cardiovascular disease. Multivariate-adjusted hazard ratios (HRs (95% confidence interval)) for all-cause mortality were significantly higher for individuals with both subclinical hyperthyroidism (HR, 3.0 (1.5-5.9); n=14) and subclinical hypothyroidism (HR, 2.3 (1.2-4.4); n=13) than for euthyroid subjects. Cardiovascular mortality was significantly associated with subclinical hyperthyroidism (HR, 3.3 (1.4-7.5); n=8), but not with subclinical hypothyroidism (HR, 1.6 (0.6-4.2); n=5). Conclusion: In the Japanese-Brazilian population, subclinical hyperthyroidism is an independent risk factor for all-cause and cardiovascular mortality, while subclinical hypothyroidism is associated with all-cause mortality.

Self-reported Body Changes and Associated Factors in Persons Living with HIV

The study aimed at verifying the associated factors of self-perceived body changes in adults living with HIV in highly-active antiretroviral therapy (HAART) in the city of Sao Paulo, Brazil. This cross-sectional study was conducted among people living with HIV on HAART for at least three months. A standardized questionnaire was used for assessing self-perceived body changes. Associated factors relating to self-reported body changes in people living with HIV (PLHIV) were assessed with Student`s t-test and chi-square test. In total, 507 patients were evaluated. The mean time since diagnosis was 6.6 years [standard deviation (SD)+/-4.1], and the mean duration of HAART was 5.1 years (SD+/-3.3). Self-perceived body changes were reported by 79.5% of the participants and were associated with viral load and duration of HAART. Fibre intake was lower among males who gained in abdominal fat (p=0.035). HAART-related body changes were reported by the large majority of the population and were associated with demographic and clinical variables.

Chronic colitis associated with HIV infection can be related to intraepithelial infiltration of the colon by CD8+T lymphocytes

Gastrointestinal complications in AIDS patients with diarrhoea are common clinical manifestations, frequently diagnosed by colonoscopy as non-specific colitis. We retrospectively study colon biopsies diagnosed as chronic colitis associated with HIV (CCH). Biopsies were sorted as patients with AIDS (serum CD4 < 200 cell/mm(3)) but without any clear infectious process (n = 12) and patients without HIV infection (n = 24). There are low numbers of CD4+ T lymphocytes in lamina propria of AIDS patients, but CD8+ T populations in this area appear to be similar in all studied groups, regardless of HIV infection or laboratory evidence of a specific agent. We found the clear evidence of CD8+ T cells infiltration in colonic mucosa in HIV patients with microscopic colitis. An imbalance of lymphocyte subpopulations in the colon, both in the lamina propria and epithelium, could result in an intraepithelial CD8 infiltration, involved in the pathogenesis of CCH in AIDS patients.

Akt Expression is Diminished by Physical Inactivity in Early Stages of Development in Soleus Muscle of Rats

Metabolic Syndrome is a group of conditions related to obesity and physical inactivity. Little is known about the role of physical inactivity, in early stages of development, in the susceptibility to insulin resistant phenotype induced by high fat diet. Akt plays a key role in protein synthesis and glucose transport in skeletal muscle and has been regulated by muscle activity. The objective of present study was to determine the effect of early physical inactivity on muscle growth and susceptibility to acquire a diabetic phenotype and to assess its relationship with Akt expression. Forty Wistar male rats were distributed in two groups (standard group, Std) and movement restriction (RM). Between days 23 and 70 after birth, RM group was kept in small cages that did not allow them to perform relevant motor activity. From day 71 to 102 after birth, 10 rats of each group were fed with hyperlipidic diet (groups Std-DAG and RM-DAG). No differences were observed in total body weight although DAG increased epididymal fat pad weight. RM decreased significantly the soleus weight. Insulin-mediated glucose uptake was lower in RM-DAG group. Akt protein levels were lower in RM groups. Real time RT-PCR analysis showed that movement restriction decreased mRNA levels of AKT1 in soleus muscle, regardless of supplied diet. These findings suggest that early physical inactivity limits muscle`s growth and contributes to instauration of insulin resistant phenotype, which can be partly explained by dysregulation of Akt expression.