Envelhecimento acelerado de sementes de espécies oleráceas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Eletrical conductivity and deterioration of soybean seeds exposed to different storage conditions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Electrical conductivity and mineral composition of the imbibition solution of bean seeds during storage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relationship of black layer and milk line development on maize seed maturity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Qualidade fisiológica de sementes de seringueira (Hevea brasiliensis Muell. Arg.), tratadas com benlate durante o armazenamento

A ocorrência de um período relativamente frio e seco, imediatamente após o período de coleta de sementes de seringueira - Hevea brasiliensis Muell. Arg. (março-abril), levou à realização do presente trabalho com o objetivo de encontrar condições adequadas de preservação da qualidade fisiológica das sementes. Assim, estudou-se a qualidade fisiológica de sementes, tratadas com Benlate (0,1%), e armazenadas em sacos de polietileno transparente, por períodos de 0 a 5 meses, sob condições ambientais de laboratório. Para a avaliação da qualidade fisiológica das sementes, determinou-se a germinação e o vigor através do índice de velocidade de emergência (IVE) e do comprimento da parte aérea das plântulas (CPA), em 1987 e 1988. A germinação e o IVE foram obtidos sob duas condições ambientais diferentes, ambiente do laboratório e em germinador a 30°C. O tratamento fungicida utilizado não exerceu efeito significativo na preservação das sementes. A germinação, o IVE e o CPA reduziram-se com o prolongamento do armazenamento. Os valores da germinação foram baixo desde o início dos trabalhos, o que pode ser atribuído ao teor de água inicial, que estava abaixo de 30%. O teste de germinação de sementes dessa espécie pode ser feito utilizando-se areia como substrato, a 30°C e com a contagem final aos 20 dias após a instalação do teste.

Efeito do vigor e do tratamento fungicida nos testes de germinação e de sanidade de sementes de soja

Soybeans seeds with different levels of vigour, treated and or no with fungicide were submitted to the test of germination in the sand and roll substrates of paper and the test of sanity. In test of germination, there were only significant differences between treatments that used soybeans with high vigour and with low vigour, regardless of the presence or absence of fungicide treatment, in the two substrates. The soybeans with low vigour no treated provided the largest percentages of seed contaminated with fungi, in contrast, the seeds with high vigour treated. There was only a slight contamination in soybeans with low vigour treated, unlike seeds with low vigour untreated, which showed the incidence of most fungi found. In the germination of seedlings normal, there are differences between treatments that used seeds with high vigour and low vigour, and is independent of the fungicide treatment and the type of substratum used. The effect of a fungicide depends of the vigour of seeds when of the occurrence of fungi associated to the soybean seeds of the cultivar analyzed.

Potencial fisiológico de sementes de mutambo (Guazuma ulmifolia Lam.) em diferentes procedências

The work had as objective to evaluate the provenances of seeds to verify the efficiency of different tests of vigor for identification of the physiological potential in four lots of seeds of mutamba (Guazuma ulmifolia Lam.). The study it was developed in the Laboratory of Analysis of Seeds and in the Experimental Fishery of Ornamental and Forest Plants - UNESP, Jaboticabal, SP. Four lots of seeds proceeding from three São Paulo localities had been used: lots I and II, collected in the city of Jaboticabal in 2000 and 2001, respectively, lot III: collected in Saint Rita do Passo Quatro and it crowds IV: collected in Luiz Antonio, both in 2001. The seeds used in all the tests had been escarificadas with sulfuric acid during 50 minutes, and washed in current water during 15 minutes. They had been evaluated: seed moisture, test of germination, test of first counting, index of germination speed, emergency in fishery, index of emergency speed. The seeds moisture of the seeds in the different lots had presented small variations (9,2 10.0%). Statistical differences for the germination percentage, emergency in fishery and index of speed of emergency in the different studied lots had not been verified. The tests of first counting and index of germination speed had revealed adequate for evaluation of the physiological potential of the lots. The seeds of lot III proceeding from the city of Santa Rita do Passo Quatro, had presented superior physiological potential to the too much studied lots.

Efeito do tratamento químico sobre a conservação de sementes de milho durante o armazenamento

O objetivo do trabalho foi estudar o efeito de combinações de inseticidas e de fungicidas sobre a conservação de sementes de milho durante o armazenamento. Os tratamentos aplicados e respectivas doses, para 1000kg de sementes, foram os seguintes: 1) testemunha; 2) anilina (936mL); 3) anilina (936mL) + Thiabendazole (120g) + Dicarboximida (187,5g) + Pirimifos- methyl (6,25mL) + Deltamethrin (0,75mL); 4) anilina (936mL) + Thiabendazole (240g) + Dicarboximida (375,0g) + Pirimifos- methyl (12,50mL) + Deltamethrin (1,50mL); 5) anilina (936mL) + Thiabendazole (480g) + Dicarboximida (750,0g)+ Pirimifos- methyl (25,00mL) + Deltamethrin (3,00mL); 6) anilina (936mL) + Thiabendazole (960g) + Dicarboximida (1500,0g) + Pirimifos- methyl (50,00mL) + Deltamethrin (6,00mL). As sementes foram analisadas quanto ao teor de água, germinação e vigor (testes de envelhecimento acelerado e de frio com solo) aos 0, 6, 9 e 12 meses de armazenamento. Os dados obtidos permitiram concluir que os tratamentos químicos aplicados tendem, com o aumento das dosagens, a gerar efeitos latentes, desfavoráveis ao desempenho das sementes, intensificados com o prolongamento do período de armazenamento.

Efeito dos substratos e temperaturas na germinação de sementes de Cnidosculus phyllacanthus Pax & K. Hoffm. (Faveleira)

Este trabalho foi conduzido com o objetivo estudar diferentes substratos e temperaturas para germinação de sementes de faveleira. Foram testadas as temperaturas constantes de 25º e 30ºC e alternadas com amplitudes de 5ºC (30-35ºC), 10ºC (20-30º e 25-35ºC) e 15ºC (20-35ºC), e fotoperíodo de oito horas para a temperatura mais elevada e dezesseis horas para a temperatura mais baixa. Os substratos papel de filtro, areia, vermiculita e papel germitest, foram umedecidos com solução de nistatina 0,2%. Para o teste de germinação em laboratório para sementes de faveleira pode-se recomendar os substratos areia, vermiculita, papel germitest e papel filtro combinado com temperaturas alternadas de 20ºC -30ºC. Para o teste de vigor, velocidade de germinação de sementes de faveleira, pode-se recomendar o substrato papel filtro combinado com as temperaturas alternadas de 20ºC -30ºC.

Teste de condutividade elétrica para sementes de mamoneira

The aim was to study the efficiency of electrical conductivity test in evaluating the quality of castor seeds. Emergence of seedlings was evaluated in the field, with five seed lots of cv. AL Guarany 2002. The seeds were submitted to the following tests: germination; first count of germination; accelerated aging (45 degrees C by 24 to 100% UR), emergence of seedlings in the field, rate of speed of development of seedlings and electrical conductivity, testing the periods (2, 4, 6, 8 and 24 hours) and the number of seeds (25,50 and 75) in 75ml of distilled water conditioning to 25 degrees C. A completely randomized design was used. The averages were compared by the Tukey test at 5%. The mean laboratory and field test data were correlated. Electrical conductivity test for 4 hours with 25 seeds and 6 hours with 50 seeds, and the rate of speed emergency proved efficient in the selection of lots vigor by providing information equivalent to the emergence of seedlings in the field.

In vitro acaricidal activity of neem (Azadirachta indica) seed extracts with known azadirachtin concentrations against Rhipicephalus microplus

The effect of four extracts from neem seeds (Azadirachta indica) containing 2000, 5000, 9000 and 10,000 ppm of azadirachtin A (AZA), quantified by high-performance liquid chromatography (HPLC) and diluted to 1.25%; 2.5%; 5.0%; 10.0% and 12.8% was verified by in vitro tests with engorged females and larvae of the cattle tick Rhipicephalus micro plus. The results from the bioassays with the engorged females showed that the main toxic effect of the extracts was reduction of the reproductive parameters, with a sharp drop in the number of eggs laid and the hatching rate, mainly when the extracts were diluted to 10.0% and 12.8%. The product effectiveness (PE) calculations for all the solutions tested showed that the AZA solution at 10,000 ppm (N10) was the most effective. However, statistical analysis of the PE data obtained for the proportional AZA concentrations in the different diluted extracts showed significance (P<0.05) of the effects included in the model (extract dilution, principle effect (classificatory) of the assay (extract) and the interaction between the two), indicating significant variations due to the dilution, the test and the interaction between the two factors in the tests with engorged females. For solutions N2, N5, and N9, it was not possible to estimate LC(90) values in the dilution range tested. The lowest LC(50) was observed for extract N5, and although extract N10 was the only extract for which the LC(90) could be estimated within the range tested, the LC(50) was higher than for N5 and N9. These results suggest that substances other than AZA present in the extracts influenced the efficacy, especially up to a certain LC range. In the tests with larvae, no mortality was observed, indicating zero effectiveness of all the extracts tested. The results of the tests with engorged females showed that the neem extracts had acaricide activity, inhibiting egg laying and the larval hatching rate. Complementary studies are necessary to develop new methods to isolate and/or identify other substances besides AZA contained in this plant, to enable using products made from it as acaricides. (C) 2011 Elsevier B.V. All rights reserved.

Procedimento para condução do teste de envelhecimento acelerado em sementes de algodão

The evaluation of the effect of seed has been fundamental tool in the program of quality control, and the accelerated aging test, an important part of that process. Some authors report that the germination and vigor are associated with seed size and facing the lack of consensus for the use of this test methodology and the relationship of seed size on the physiological quality, this work aimed to adapt the existing methodology as the temperature and period of conditioning on the performance of two sizes of seed cotton. The seeds were classified into two meshes of sieves, and then subjected to tests of water content, germination and electrical conductivity. The experimental design was completely randomized, analyzed statistically in a factorial threefold. Seeds of different sizes, showed no difference in percentage of germination. Seeds subjected to 40 degrees C for 42h of conditioning showed better percentage of germination.

Tamanho e secagem de sementes de palmeira Jussara sobre a germinação e o vigor

With the objective to determine the influence of the dehydration and of the size and/or weight on germination percentage and the vigor of jussara palm seeds (Euterpe edulis Mart.), 10 individuals' ripe fruits, picked in the Experimental Station of Ubatuba (IAC) they were classified by size in 5 sieves of circular sieve: 26/64" (10,31mm), 28/64" (11,11mm), 30/64" (11,90mm), 32/64" (12,69mm) and 34/64" (13,49mm). It was determined, the medium weight of the classified seeds. The quality of the seeds was evaluated through the following parameters: water content of the seeds; germination and vigor (first germination counting). It was evaluated the drying speed of different seed sizes for 48 hours, in dry chamber. The sizes of 28/64", 30/64" and 32/64" represented 98,7% of the total weight of the harvested seeds and showed similar drying speed, percentage of germination and vigor. With the increase of the drying period, the smaller seeds (28/64") had their germination less affected them the intermediate (30/64") and bigger sized seeds (32/64").

Efficiency of seed treatment with fungicides in castor bean crop genotypes

With the objective of assessing castor bean genotypes in relation to the influence of seeds treatment on agronomic characteristics and physiological quality of seeds, two experiments were conducted between November, 2005 and March, 2006. In the first study, experimental design used was randomized complete blocks, in factorial scheme (2 x 10), by comparing Lyra hybrid and AL Guarany 2002 cultivar and nine fungicides, including its mixtures and test, with four repetitions. Plant height, stem diameter, medium yield and observation of diseases incidence were evaluated. In the second study, conducted in randomized entirely design, in factorial scheme 2 x 10 with four repetitions, Savana and AL Guarany 2002 genotypes were analyzed, using the same fungicides. Germination, infected and dead seeds, vigor, initial moisture content and simulation of field emergency and seeds sanitary quality were also evaluated. The results obtained point that there is positive influence of seed treatment on agronomic characteristics in castor bean crop, increasing grain yield for AL Guarany cultivar and Lyra hybrid. In the analysis of seed quality, it was observed that seed treatments with fungicides and their mixtures provided superior percentages of germination and vigor, reduced the number of infected and dead seeds and increased seeds emergency for Guarany 2002 cultivar and Savana hybrid.

Seletividade de herbicidas aplicados em pós-emergência sobre capim-colonião e efeito na qualidade das sementes: effects on seed germination

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)