第6章 取引額行列の推計におけるRAS 法の特徴

取引額表においてすべての要素は知られておらず、行および列の合計がベクトルとして既知であるとき、適当な行列を初期値にすることにより取引額の要素を推計することができる。比例反復法あるいはRAS 法は、繰り返し計算によって解を求めるそのような推計方法である。本章は正数を要素とする行列X (0) に対して任意の正数を要素とするベクトルa とb、D(a) をa を要素とする対角行列とするとき、初期値をX (0) と(0) ( ) (0) ( )X1 = D a X D b としたRAS 法による解は一致することを示す。RAS 法の解はその初期値においてD(a) とD(b) に対して無関係である。

Repetitio. ru. [et] ca. p[er] v[est]ras de donationib[us] int[er] virum [et] vxorem / edita a Joa[n]ne lupi de palacios ruuios ...

Sign.: []2, a-z8, [et]8, [cum]8, [rum]8, 2a-2d8, 2e-2f6, A10, a8, b10

Influence of the training system on the agronomic behavior and grape composition of Albariño in Rías Baixas apellation

The aim of this study was to evaluate if early defoliation can be an alternative to bunch thinning in limiting yield and improving quality in grapes of the white cultivar Loureiro (Vitis vinifera L.), grafted onto 1103P. The field trial had been set up in a commercial vineyard in Vinhos Verdes Region (Northwest of Portugal, 41º 48? 53? N, 8º 24? 42? W). Treatments studied, performed five days before full bloom were: LR5 ? Leaf removal of the first five basal leaves, performed manually, LR8 ? Leave removal of the first eight basal leaves, LRM ? mechanical leaf removal and C ? the control, without defoliation. This paper reports the results of four years (2010-2013). The results presented a significant removal of main leaf area after defoliation principally in the most intensive treatment (LR8) but at harvest, the total leaf area had been compensated by lateral regrowth and no statistical differences between the treatments and the control were found. Early defoliation caused a decrease in fruit set and also a significant reduction in the diameter of the berry within the more severe defoliation treatments (LR5 and LR8). Yield factors were also significantly affected by the defoliation, causing a reduction of bunch weight and in 2013 a yield reduction in LR8 and LRM, and in 2010 in LR8. Conversely, LR5 presented a yield always similar to the control C. The reduction of cluster compactness and the substantial improvement of the microclimate at the cluster level significantly reduced bunch rot incidence in the defoliated modalities compared to control. No carry-over effects, along the four years trial were observed Early defoliation proved to be a canopy management technique that can have a strong impact in the final quality of grapes, reducing the compactness and lower the incidence and intensity of bunch rot, even if the reduction of yield observed in other papers had not been observed in all modalities.

Alloantigen-induced unresponsiveness in cord blood T lymphocytes is associated with defective activation of Ras

Human umbilical cord blood T lymphocytes (CBTL) respond to primary allostimulation but they do not proliferate upon rechallenge with alloantigen. Using PKH-26-labeled cells created a proliferative block that was observed only in CBTL that have divided during primary stimulation (PKH-26dim) but not in unstimulated (PKH-26bright) CBTL. CBTL’s secondary unresponsiveness resembles anergy and can be overcome by treatment with phorbol myristate acetate (PMA) and ionomycin or by high doses (50–100 units/ml) of interleukin 2. Addition of interleukin 2 to the primary cultures does not prevent the induction of secondary unresponsiveness. Defective Ras activation is detected in PKH-26dim CBTL during secondary response to alloantigen or after antibody-mediated T cell receptor stimulation whereas Ras is activated and proliferation is induced in CBTL during primary alloantigenic stimulation. Upon stimulation with PMA plus ionomycin, PMA plus alloantigen, but not alloantigen plus ionomycin, Ras is activated in PKH-26dim CBTL, and the block in proliferation is overcome. Correction of PKH-26dim CBTL’s proliferative defect correlates with PMA-induced Ras activation, suggesting a defect in the signaling pathway leading to Ras. Ras-independent signals, necessary but not sufficient to induce PKH-26dim CBTL proliferation, are provided by alloantigen exposure, as evident by the ability of PMA plus alloantigen but not PMA alone to overcome the proliferative block. Functional signal transduction through CD28 in PKH-26dim CBTL is supported by detectable CD28-mediated PI-3 kinase activation after PKH-26dim CBTL’s exposure to alloantigen or CD28 cross-linking. These results suggest that defective activation of Ras plays a key role in PKH-26dim CBTL’s secondary unresponsiveness and point to a defect along the T cell receptor rather than the CD28 signaling pathway.