Hematology of free-living marsh deer (Blastocerus dichotomus) from Southeast Brazil

This work reports basic hematologic values of a sample of a population of free-living marsh deer (Blastocerus dichotomus) living by the Parana River in Southeast Brazil. Hematologic values are presented separately for male, female, and young animals as well as for anesthetized and nonanesthetized cervids. Nonanesthetized deer restrained by physical means had significantly higher erythrocyte indices and total leukocyte counts. Comparisons of blood parameters of anesthetized animals of different ages and gender differed slightly, with only two significant differences observed: young animals had significantly higher red blood cell counts than adult males and a lower blood total protein content when compared to adult females. Results indicate that two main reference ranges for blood values should be considered for marsh deer, for blood obtained from anesthetized or physically restrained individuals.

Species diversity and seasonality of free-living ticks (Acari : Ixodidae) in the natural habitat of wild Marsh deer (Blastocerus dichotomus) in Southeastern Brazil

This study evaluated the presence and seasonal activity of free-living ticks in remaining marsh areas by the Parana river, in Brazil. Eight field trips (once per season) for collection of ticks were performed during 2 years. Using CO2 traps, dragging, and visual inspection of vegetation, five free-living tick species were collected, in the following order of abundance: Amblyomma cajennense, Amblyom\ma dubitatum, Amblyomma triste, Amblyomma coelebs, and Amblyomma nodosum. The seasonal pattern of A. cajennense was characterized by the highest peaks for adult ticks in the summer/spring months, for nymphs in the winter and for larvae in the autumn and winter. A. dubitatum and A. triste presented similar seasonal patterns characterized by peaks of adult ticks in the autumn. Nymphs of A. dubitatum peaked in the winter of the first year and in the winter/spring of the second year. A. triste was the only species to be collected in significantly higher numbers in the marsh than in surrounding drier areas such as forest patches. Among domestic animals living close the marsh areas, horses were infested by Anocentor nitens, A. cajennense, and Boophilus microplus, bovines were infested solely by B. microplus, and dogs were infested by Rhipicephalus sanguineus. Adults of A. triste showed to be well adapted to the marsh environment. This result, at least partially, explains local previous observations on the association of A. triste with marsh deer, as this vertebrate host inhabits mainly the marsh area. (c) 2006 Published by Elsevier B.V.

Prevalence and molecular characterization of Anaplasmataceae agents in free-ranging Brazilian marsh deer (Blastocerus dichotomus)

Anaplasmataceae organisms comprise a group of obligate intracellular gram-negative, tick-borne bacteria that can infect both animals and humans. In the present work we investigate the presence of Ehrlichia, Anaplasrna, and Neorickettsia species in blood samples from Brazilian marsh deer (Blastocerus dichotomus), using both molecular and serologic techniques. Blood was collected from 143 deer captured along floodplains of the Parana River, near the Porto Primavera hydroelectric power plant. Before and after flooding, marsh deer were captured for a wide range research program under the financial support of São Paulo State Energy Company (CESP), between 1998 and 2001. Samples were divided into four groups according to time and location of capture and named MS01 (n = 99), MS02 (n = 18) (Mato Grosso do Sul, before and after flooding, respectively), PX (n = 9; Peixe River, after flooding), and AGUA (n = 17; Aguapei River, after flooding). The seroprevalences for Ehrlichia chaffeensis and Anaplasma phagocytophilum were 76.76% and 20.2% in MS01, 88.88% and 5.55% in MS02, 88.88% and 22.22% in PX, and 94.12% and 5.88% in AGUA, respectively. Sixty-one animals (42.65% of the total population) were PCR-positive for E. chaffeensis PCR (100.0% identity based on 16S rRNA, dsb, and groESL genes). Seventy deer (48.95% of the total population) were PCR-positive for Anaplasma spp. (99.0% of identity with A. platys, and in the same clade as A. phagocytophilum, A. bovis, and A. platys based on 16S rRNA phylogenetic analysis). Our results demonstrate that Brazilian marsh deer are exposed to E. chaffeensis and Anaplasma spp. and may act as reservoirs for these rickettsial agents, playing a role in disease transmission to humans and other animals. (c) 2012 Elsevier Ltd. All rights reserved.

NEST and EGGS of THE MARSH ANTWREN (STYMPHALORNIS ACUTIROSTRIS): THE ONLY MARSH-DWELLING THAMNOPHILID

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cytogenetic analysis of the Marsh Deer, Blastocerus dichotomus (Mammalia, Cervidae)

The chromosomic constitution of the Marsh Deer (Blastocerus dichotomus) was studied in 18 males and 18 females, mainly from the Tiete river basin in Sao Paulo State, Brazil. The species diploid number was determined to be 66 chromosomes and the fundamental number (FN), 74. The X and the Y were the largest and the smallest chromosome, respectively. Large amounts of the constitutive heterochromatin marked by the C band were located in the centromeric region of all the acrocentric chromosomes. The first chromosome pair was not marked and the second and third pairs showed weak centromeric markings. The X chromosome showed two strong telomeric markings while the Y was C band negative. Chromosomes four and five were the NOR carriers. Polymorphism for this band was observed in pair four. The results of this study are in agreement with other reports in the literature, in spite of the different origin of the animals.

Synergistic actions of 50°C water and imazalil dip treatments to preserve quality of late-season 'Marsh' grapefruit

Late-season grapefruits (Citrus paradisi Macf. cv. Marsh seedless) were dipped in water at 50°C for 3 min with and without 200 ppm imazalil (IMZ) or 1000 ppm IMZ at 19°C and were subsequently stored at 7°C and 90-95% relative humidity (RH) for 11 weeks plus one week at 21°C and approximately 75% RH to simulate a marketing period (SMP). Residue concentrations in fruit after treatment with 200 ppm IMZ at 50°C were 3.46 ppm, about twice the level (1.80 ppm) found in fruit treated with 1000 ppm IMZ at 19°C. Fungicide degradation rates during storage showed similar patterns resulting in an approximately 50% decrease. Both fungicide treatments significantly reduced decay and chilling injury (CI) during storage and SMP. Hot water reduced CI and decay but not as effectively as the IMZ treatments. Soluble solids concentrations were not affected by treatments, IMZ treatments resulted in significantly lower values of titratable acidity and higher concentrations of ethanol in the juice after SMP. Weight loss was significantly higher in fruit dipped in water at 50°C after SMP. No visible damage occurred to the fruit as a result of any of the treatments.

Comparison of artificial and natural, new and reused, brackish water for the larviculture of the freshwater prawn Macrobrachium rosenbergii in a recirculating system

Differences in culture duration, metamorphosis rate and the productivity in hatchery culture of M. rosenbergii using a closed system with natural and artificial brackish water were evaluated. Reuse of brackish water in more than one hatchery cycle was also evaluated. Natural and artificial brackish water constituted the two tested treatments, which were distributed in four independent recirculating systems (tank and respective biofilter). Four batches of cultures were conducted and the 2nd and 4th reused the water from the 1st and 3rd, respectively. Mean duration of the hatchery period was 28 d in natural brackish water and 31 d in artificial brackish water. The metamorphosis rate and the average productivity for the natural brackish water treatment were 74% and 60 postlarvae/ L. respectively, and values obtained with artificial brackish water were 55% and 44 postlarvae/L. The successful hatchery culture of M. rosenbergii in this specific artificial brackish water suggests its potential use in enterprises located far from the coast. Brackish water can be used in two consecutive cultures without a negative effect on productivity.

Ticks (Acari: Ixodidae) on Wild Marsh-Deer (Blastocerus dichotomus) from Southeast Brazil: Infestations before and after Habitat Loss

The lake from Porto-Primavera hydroelectric power station inundated an area of 2,200 km2 at the border of São Paulo and Mato-Grosso do Sul States, Brazil. Infestations by ticks were evaluated on 135 marsh deer, Blastocerus dichotomus (Illiger), captured before and after inundation. Ticks were collected for identification, and infestation level of animals was assessed by scoring. Deer were divided into four groups according to capture location and temporal relation to the inundation. Groups 1, 2, and 3 were captured before inundation. Group 4 was captured after inundation. Four tick species were found: Amblyomma cajennense (F.), Amblyomma triste Koch, Anocentor nitens (Neumann), and Boophilus microplus (Canestrini). Groups 1, 2, 3, and 4 had 30, 45, 100, and 96%, respectively, of animals carrying B. microplus ticks. A. triste was observed on 16, 22, 22, and 88% of animals from groups 1,2,3, and 4, respectively. A. nitens and A. cajennense were observed only on group 4, on 32 and 16% of the animals, respectively. Groups 1 and 2 had only 4.8 and 6.1% of animals with high infestation levels, respectively, and no ticks on 46.8% and 45.5% of the animals, respectively. Conversely, groups 3 and 4 lacked noninfested animals and had high infestation levels on 77.8 and 50% of deer, respectively. Marsh area shrinkage was blamed for higher infestation levels on deer from groups 3 and 4. The widespread presence of A. triste on marsh deer, a Neotropical tick species, raises the possibility of a natural host-parasite relationship.

Tuberculosis survey of free-ranging marsh deer (Blastocerus dichotomus) in Brazil

Esophageal-pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted, amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease.

Natural infection by Paramphistomoidea Stiles and Goldberger, 1910 trematodes in wild Marsh Deer (Blastocerus dichotomus Illiger, 1815) from Sérgio Mottas's hydroelectric power station flooding area.

Studies on helminthfauna of marsh deer Blastocerus dichotomus Illiger, 1815 are rare, although helminthic diseases are an important cause of mortality in these animals. Fifteen male and female adult marsh deer from Sergio Motta's hydroelectric power station flooding area at Paraná River which died during the capture and quarantine procedures, between 1998 and 1999, were necropsied. Three trematodes species, Paramphistomum cervi, Balanorchis anastrofus and Zygocotyle lunatum, all belonging to superfamily Paramphistomoidea, were identified. The obtained trematodes were identified, counted and their respectives descriptors of infection were determined. All necropsied animals were infected by helminths. Paramphistomum cervi was the most prevalent species, while Zygocotyle lunatum was found in only one animal.

Transferability of microsatellite loci from Cervidae species to the endangered Brazilian marsh deer, Blastocerus dichotomus

Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroelectric power station construction and agricultural land expansion. In the present study, we tested 38 microsatellite loci from four Cervidae species: Odocoileus virginianus (7), Rangifer tarandus (17), Capreolus capreolus (7), and Mazama bororo (7). Eleven loci showed clear amplification, opening a new perspective for the generation of fundamental population genetic data for devising conservation strategies for B. dichotomus. © FUNPEC-RP.

Sexual differences in parental feeding effort during the nestling period of the White-headed Marsh-Tyrant (Arundinicola leucocephala)

Parental effort may influence the fitness and survivorship of adult birds and their offspring from one breeding season to the next. Although much is known about parental feeding effort in tyrant flycatchers, many species remain understudied. In this study, I examined parental feeding effort of the White-headed Marsh-Tyrant (Awndinicola leucocephala) at a pond in southeastern Brazil. I conducted 10.3 hrs of observations from two locations: a high place with all view of the whole pond, that allowed me to observe flight distances of parents hunting for prey; and from near the nest to observe frequency of visits to the nest. The female marsh-tyrant flew farther while hunting prey and made more visits to the nest than did the male. The adults (mainly the female) provided a variety of terrestrial and aquatic arthropods to the nestlings. Additionally, parents removed fecal sacs from the nest and nestlings eliminated arthropod remains from the nest, the first records of nest sanitation activities by this species. Nonexclusive hypotheses that may explain the lower level of parental care provided by the male include: higher predation risk due its more conspicuous plumage, commitment of male to territory defense, and its selfish behavior influenced by indirect genotype fitness inherited by the offspring. © The Neotropical Ornithological Society.

Severe population decline of marsh deer, Blastocerus dichotomus (Cetartiodactyla: Cervidae), a threatened species, caused by flooding related to a hydroelectric power plant

Balancing power production and environmental conservation can be problematic. The objective of this study was to investigate the abundance of marsh deer in the Paraná River Basin, above the Sergio Motta (Porto Primavera) Dam, before and after the impact of the dam closure. A fixed-wing, flat window aircraft was used to survey study transects. Observations were recorded based on the distance sampling line transect method, assuming that the detection probability decreases with increased distance. The abundance of marsh deer in the survey region prior to flooding was estimated to be 974 individuals (CV = 0.23). The overall abundance dropped from 974 to 444 (CV = 0.26) individuals after flooding, an overall reduction of 54%. This reduction can be attributed to the direct impact of the flooding process, but it was likely exacerbated by indirect effects, such as increased disease, hunting, and reduction in food availability. Prior to flooding, the marsh deer was distributed widely throughout the dam's catchment area; however, the marsh deer habitat was almost completely destroyed by the flooding process. This situation highlights the need to implement management strategies that ensure the survival of the remaining fragmented population.

Integrated multi-trophic culture of Nile tilapia (Oreochromis niloticus) and Amazon river prawn (Macrobrachium amazonicum) in brackish water

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Novel and cross-amplified microsatellite loci for the critically endangered São Paulo marsh antwren Formicivora paludicola (Aves: Thamnophilidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)