Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mites and leaf domatia: no evidence of mutualism in Coffea arabica plants

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Use of strontium for bovine oocyte activation

Strontium efficiently activates mouse oocytes, however, there is limited information on its use in cattle. Thus, the objective of this study was to establish a suitable protocol for activating bovine oocyte with strontium. For pronuclear development, the absence of calcium and magnesium in the activation medium (TALP) with 10 and 50mM strontium (34.4 and 53.1%, respectively) was superior to the complete TALP (6.5 and 19.4%, respectively). In all activation media, better results were observed with 25 and 50 mM strontium (21.9-53.1 and 19.4-53.1%, respectively). Incubation for 4 h promoted similar results in all strontium concentrations. However, strontium at 15, 20, and 25 mM for 6 and 8 h (40.7, 46.7, and 48.3%, and 29.3, 48.3, and 40.7%, respectively) were superior to control (15.5 and 10%, respectively). After in vitro maturation for 26 h, strontium (S; 20 mM in Ca2+ and Mg2+-free TALP for 6 h), ionomycin + strontium (IS), and strontium + ionomycin (SI) (60, 63.3, and 65%, respectively) were similar in pronuclear development and superior to ionomycin (I; 5 mu M for 5 min; 36.7%). In treatments S and I, only 1 PN zygotes were observed. In treatment S, most of them had 1 and 2 PB (35.7 and 60.7%, respectively), and in treatment I, 0, 1, and 2 PB (14.3, 57.1, and 28.6%, respectively). Most of the zygotes in treatment IS and SI were 1 PN 2 PB (77.4 and 61.6%, respectively). The number of oocytes with clusters of cortical granules was similar in all treated groups (11-29%). Cortical granule exocytosis in treatment IS (68%) was similar to S (54%) and superior to 1, SI, and control (27, 45, and 5.0%, respectively). Cleavage and blastocyst rates were similar for S, I, IS, and SI treatments (61.7-76.7, and 8.3-13.3%, respectively) and the same was observed for ICM, TE, and total cell number, and ICM/total cell ratio (22-25, 64-69, and 86-95, and 0.26-0.27). In conclusion, strontium may be efficiently applied for bovine oocyte activation at 20 mM in Ca2+-and Mg2+-free TALP medium for 6 h.

Desenvolvimento vegetativo de quatro espécies de grama irrigadas com efluente doméstico

This research aimed to compare the development of four grasses species irrigated by dripping with an effluent from a domestic wastewater station treatment in a small rural community, and supply water from Sabesp. For this purpose, an experiment was conducted in a greenhouse at the FCA/UNESP (Botucatu - SP) for 12 months in a completely randomized 4 × 3 factorial design, with four replications, totalizing 48 parcels. Grass species used in this experiment were: Santo Agostinho (Stenotaphrum secandatum (Walt.) Kuntze); Bermudas (Cynodum dactylon); Esmeralda (Zoysia japônica) and São Carlos (Axnopus sp). The treatments were: T1- Irrigation with re-use; T2- Irrigation with re-use + chemical fertilization and T3- Irrigation with supply water from Sabesp + chemical fertilization (control). Data were submitted to comparison of means by Tukey's test at 5 %. The results showed that nitrogen in the wastewater was enough for the good development of the grass species. However, its use caused higher potassium loss.

Desenvolvimento vegetativo de quatro espécies de grama irrigadas com efluente de estação de tratamento de esgoto doméstico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O cut-up em Naked Lunch de Willian Burroughs: a narrativa em estilhaços

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Transposição de galharia como técnica de restauração de áreas degradadas: uma avaliação da eficiência do método na atração de fauna

O estudo foi realizado numa área degradada pertencente ao campus da UNESP em Rio Claro, e teve como objetivo avaliar a eficiência do método de nucleação para recuperação de áreas degradadas do tipo “transposição de galharia” na atração de fauna. As pilhas de galhos podem funcionar como abrigos artificiais, atraindo potenciais dispersores de sementes secundários, o que potencialmente abreviaria o processo de regeneração de um ambiente degradado. O estudo foi feito através do levantamento de pequenos mamíferos por armadilhas de interceptação e queda em quatro parcelas de 2500 m², sendo duas delas com o tratamento (pilhas de galhos), e duas funcionando como controle, e o resultado esperado seria uma maior quantidade de indivíduos capturados e de gêneros taxonômicos nas parcelas com o tratamento. Entretanto, as quantidades totais de indivíduos capturados no bloco das parcelas com tratamento e no bloco das parcelas controle foram, segundo o teste de Mann-Whitman, estatisticamente idênticos. Também a quantidade de gêneros taxonômicos capturados foi igual nos dois blocos. Desta forma, concluímos que o método “transposição de galharia” foi ineficiente neste estudo, da forma como foi empregado, não contribuindo para um aumento na fauna de pequenos roedores e marsupiais. O método pode ter fracassado devido a pequena quantidade de pilhas de galhos utilizados para o tamanho da área, como também pelo recobrimento da área por uma grande quantidade de gramíneas, que podem ter funcionado por si só como abrigo para os animais capturados, além de poderinibir a entrada de outros gêneros de pequenos mamíferos não-voadores. Sugerimos portanto, para o método ser mais eficiente, a colocação de uma maior quantidade de pilhas de galhos do que a utilizada no presente estudo, e elas devem ser maiores. Para ecossistemas que apresentam gramíneas densas e altas... (Resumo completo, clicar acesso eletrônico abaixo)

A ideologia dos filmes utilizados na educação infantil: uma análise a partir da teoria crítica

This work has as purpose to show what are the influences of media in the education and in the shaping the thinking and understand that ideologies are present in the films used in the classroom in kindergarten. For this, I directed the discussion beginning by concepts as Ideology and its more different definitions by several thinkers over the years, the thinkers of Critical Theory of Frankfurt School, called “frankfurtians” of first and second generation of this school, also making an association between Cinema and Education, evidencing concepts involving this association as Cultural Industry, using films properly in classrooms in kindergarten, with the teacher as mediator and then discussed what amounts of movies on children's education, how they assist and deepen the knowledge developed by students in schools of infantile education. Additionally, they are exposed the different opinions about the subject Film and Education for students from kindergarten, what benefits and what disadvantages in the use of films, the different opinions on the subject. We also discuss, in this work, the ideologies present in three films produced by Walt Disney, they are Cinderella, Snow White and the Seven Dwarfs and Alice in Wonderland, relating them to how they can contribute to the development and learning students in kindergarten. I associated more as possible the previous discussions to analyze the ideologies present in these films, by summarizing these, and decomposing them through an analysis tool called frame. Finally, I completed everything that was discussed and analyzed, which students in kindergarten may develop with the use of films in schools, what they contribute, in some way, for the learning and education of students as viewers using the school as access to different cultures and the teacher as a mediator of this relationship

Valente: o cinema como ferramenta para a discussão sobre gêneros em sala de aula

Este texto pretende analisar o filme de animação Valente, produzido pela Walt Disney Pictures e pela Pixar Animation Studios, em busca de elementos que abordem o tema “gênero” e, assim, utilizá-lo de modo a propor a discussão em sala de aula sobre este mesmo tema, levando em consideração o contexto atual da imagem da mulher e pontos históricos que sejam interessantes ao debate

O estilo do rei: o diálogo entre O Rei Leão, da Disney, e Hamlet, de Shakespeare, sob uma perspectiva semiótica

This work consists of a semiotic analysis of the animation motion picture The Lion King (1994), by the Walt Disney Animation Studios, describing its intertextual relation with Hamlet, by Shakespeare, considering Disney’s individuality, its style. In order to study style in texts, we use discursive semiotics theory, highlighting Discini’s work (2013) and the concept of discursive settings. Hence, a deep discussion about The Lion King’s style, compared to the Shakespeare’s play, is established. The movie, once a syncretic text, requires advanced studies on Expression Plane, its plastic, musical and verbal/phonic aspects. We find these studies in the work of José Luiz Fiorin (2009), Lúcia Teixeira (2009), Ana Claúdia de Oliveira (2009), Jean-Marie Floch (2009) and Antônio Vicente Pietroforte (2008). We note how a syncretic text makes the discursive settings more complex by assembling plastic and sonorous materials in semissimbolic relation. Once defined The Lion King’s style, we analyze the way it justifies the intense popularity of this kind of animation features. Finally, it is important to understand how Disney uses in its style not only discursive settings, but also passional settings, revealing its own way to stir emotions on the spectator

Estudo das categorias narrativas, variações e permanências nas versões de Basile, Perrault, Grimm e Disney de A Bela Adormecida

Pós-graduação em Estudos Literários - FCLAR

O American Israel Public Affairs Office Committee (AIPAC) e sua influência na política externa dos Estados Unidos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effects of plant regulators on the growth and flowering of Saint Augustine grass plants

The objective of this work was to evaluate the effects of three plant growth inhibitors on the development and emission of floral rachis of Saint Augustine grass [Stenotaphrum secundatum (Walt.) Kuntzel] plants. The study was carried out in a 15 month old lawn with the experimental plots being distributed in accordance with a complete randomized block design with four replications. The treatments consisted of sole application of trinexapac-ethyl (113, 226, 452, 678, and 904 g ai ha(-1)), prohexadione-calcium (100 and 200 g ai ha(-1)), and bispyribac-sodium (40 and 60 g ai ha(-1)) plus a check treatment in which the plants were not submitted to any of the plant growth inhibitors. The effects of those products were evaluated in terms of visual signs of plant intoxication, plant height, emission and height of floral rachises, and chip total dry matter production. All the plant growth inhibitors resulted in visible injury to the plants but these intoxication signs practically disappeared 28 days after the application. Trinexapac-ethyl at the dose of 904 g ai ha(-1) reduced plant height by 59.7%, the emission of floral rachis by 96.4%, and the amount of chip dry matter production by 87.7%. Plant growth inhibitors may reduce the number of times of lawn plants cutting up to 119 days after their application with no harmful effects on the plants visual aspect.

Estúdio Ghibli: um aparato sobre as técnicas ilustrativas e filosofia oriental dos principais longas metragens de Hayao Miyazaki

In front of the vast and widespread use of new technology in 3D animation currently, this research aims to give an overview adjacent to major film productions with Studio Ghibli and its main director/animator: Hayao Miyazaki. Showing up as an interesting exception to worldwide success, the Ghibli feature films are made with the predominance of 2D hand-drawn art. Its process dates back to the first animations created in the early twentieth century, though such tools have been “overcome” by big studios like Walt Disney, Pixar and Dreamworks, the works of Miyazaki still get considerable highlight, preferring the pencil and paper than the computer. With the aid of some authors in particular as McCloud and the founders of Anima Mundi, and other theorists of design and subjective philosophy, some analyzes are mapped to better understand the connection between the work of Miyazaki and his contribution to the field of illustration and originality as a whole. The objective is to find the key points of Ghibli animations which will drive new parameters between creativity, illustration and Western and Eastern praxis.

Block & Bridle Annual 1956-1957

Charles Adams (Faculty Advisor), Delbert Kuhlman, John Klingenberg, Ardyce Haring, Harvey Jorgensen, Roy Volzke, Billie Reed, Paul Yeutter Prof. Loeffel, Carolyn Hall, Byron Kort, Larry Paul, Elwin Mosier, Charles Corkle, Kay Robohm, Daniel Stilwell Duane Stokebrand, Gary Briggs, Walt Patterson, Wendell Mousel, Keith Smith, Darrel Zessin, Richard Bonne, Donald Kasbohm, Bruce Skinner Bob Discoe, Doyle Hulme, Jim Smith, Carl Lorenzen, Jay Cook, Gary Berke, Bob Volk, Roger Hild Donald Kuhl, Russell Person, Ray Cada, Ray DeBower, Bob Dannert, Phil Starck, Kay Knudsen, Jerry Brownfield, Allan McClure, Wally Bierman Morris Ochsner, Warren Mitchell, Ed McReynolds, Gerald Dart, Arza Snyder, Mervy Schliefert, Arley Waldo, Tom Hoffman, John Wink, Virgil Gellermann, Duane Neuman