New mitochondrial and nuclear evidences support recent demographic expansion and an atypical phylogeographic pattern in the Spittlebug Philaenus spumarius (Hemiptera, Aphrophoridae)

Copyright: © 2014 Rodrigues et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Geotecnia urbana da zona ribeirinha de Vila Nova de Gaia: criação de uma base de dados SIG geotécnica

O presente trabalho apresenta os resultados dos estudos geotécnicos e de uma base de dados da zona ribeirinha de Vila Nova de Gaia, com o objectivo de compreender melhor os aspectos geotécnicos em ambiente urbano numa área sensível com um registo histórico de instabilidade de taludes rochosos. Além disso, os escassos estudos científicos recentes de natureza geológica e geotécnica em Vila Nova de Gaia justificam o estudo exploratório da geotecnia urbana da zona ribeirinha de Vila Nova de Gaia. A importância de Vila Nova de Gaia como a terceira maior cidade portuguesa e como centro de intensa actividade económica e cultural despoleta uma constante necessidade de expansão. O aumento da densidade populacional acarreta a realização de projectos complexos de engenharia, utilizando o subsolo para a construção e, com frequência, em terrenos com características geotécnicas desfavoráveis. As cidades de Vila Nova de Gaia e do Porto foram sendo edificadas ao longo de encostas numa plataforma litoral caracterizada por uma vasta área aplanada, inclinando ligeiramente para Oeste. Esta plataforma foi cortada pelo Rio Douro num vale encaixado de vertentes abruptas, nas quais se localizam as zonas ribeirinhas das duas cidades. Este trabalho envolveu, inicialmente, uma caracterização topográfica, morfoestrutural, geotectónica e geomecânica da área de estudo e, numa fase posterior, o desenvolvimento duma base de dados geotécnica. Todos os dados geológicos e geotécnicos locais e os estudos geotécnicos levados a cabo in situ pelas diversas empresas e instituições foram representados cartograficamente numa base apoiada pelos Sistemas de Informação Geográfica (SIG). Esta metodologia inter‐disciplinar foi de grande valor para um melhor conhecimento dos riscos geológico‐geotécnicos ao longo das margens do Rio Douro. De facto, a cartografia geotécnica da zona ribeirinha de Vila Nova de Gaia deve constituir uma ferramenta importante para uma previsão mais rigorosa de futuras instabilidades de taludes e um bom instrumento para a gestão do espaço urbano.

Age constraints on the late cretaceous alkaline magmatism on the West Iberian margin

Cretaceous Research 30 (2009) 575–586

B and Delta hepatitis virus infection in a population of West Africa

Among the 424 serum samples examined, the prevalence of hepatitis virus infection turned out to be 89.6%, with 15.6% of HBsAg positivity. Some of the samples belonged to an afferent population and some other to workers of a West Africa rural hospital (Pop. Rep. of Benin). 27.3% of the positive subjects presented active replication of the virus, shown by the presence of HBeAg. Among the HBcAb positive subjects the anti-delta antibodies showed a positivity frequency of 19.7%. HBsAg presence in 15% of pregnant women suggested the importance of HBV mother-foetal transmission in the district. The examined results can be compared with those obtained in other African areas, with similar socio-economic conditions.

The Cenomanian-Turonian transition in West Central Portugal: ammonites and biostratigraphy

The Upper Cenomanian and Lower Turonian ammonite assemblages from the onshore sectors of the West Portuguese Margin are reviewed after new studies on the type section of Figueira da Foz, and correlative sections of Baixo Mondego. The faunal succession shows a strong contribution of vascoceratids and other ammonites with North African and Tethyan affinities. Euomphaloceras septemseriatum (Cragin, 1893), Kamerunoceras douvillei (Pervinquere, 1907), Fagesia catinus (Mantell, 1822), Neoptychites cephalotus (Courtiller, 1860), and Thomasites rollandi (Thomas & Peron, 1889) are for the first time mentioned to Portugal. The Upper Cenomanian is recognised after a set of 3 assemblage zones: Neolobites vibrayeanus z., Euomphaloceras septemseriatum z ., and Pseudaspidoceras pseudonodosoides z. The carbonate succession shows an important unconformity across the Cenomanian-Turonian boundary, associated to subaerial exposure, and to the development of a palaeokarst over Upper Cenomanian units. The first Lower Turonian carbonates are yielded a single but diverse ammonite assemblage of middle Lower Turonian age (Thomasites rollandi z.). This biozone was previously recognised in Central Tunisia by G. Chancellor et al. (1994).

Notes on the Cretaceous invertebrate faunas of Portugal I: a new species of Anadromus (Mollusca, Gastropoda) from the Campanian-Maastrichtian of Taveiro (Coimbra, West central Portugal)

A new species of terrestrial gastropod - Anadromus penai sp. nov. (Fam. Anadromidae) - is described from a set of composite moulds collected in reddish silts and clays of Campanian-Maastrichtian age, found in the lower pan of the Taveiro Formation (Taveiro, Coimbra, West Central Portugal). The known occurrences of this new species are restricted to the type locality. The main differences from other contemporaneous Anadromidae are the profuse spiral sculpture of the body-whorl, with 20-22 sub-equal, close, and regular ribs.

Upper Paleocene-Early Eocene mollusks of Silveirinha (Figueira da Foz, West Central Portugal)

A collection of fossil gastropods and bivalves assembled at the Thanetian/Ypresian vertebrate site of Silveirinha (Figueira da Foz, West Central Portugal) is analysed from the point of view of systematics and palaeoecology. The diversity is scarce but the age and exceptional characteristics of the site are factors that substantiate a detailed study. The taxa identified are: Bithynia soaresi sp. nov., Gyraulus antunesi sp. nov., Chlamys sp. and Cardiiacea gen. sp. indet. The prevailing of freshwater gastropods and the occurrence of 2 fragments of marine bivalves suggest a palaeoenvironmental setting that is in conformity with interpretations already established, which are based both in sedimentologic and vertebrate data. These interpretations point out the existence of a freshwater environment opened from time to time to marine influences, resulting from a palaeoatlantic coast placed some kilometres westwards.

The Farol Deposit (Depósito do Farol) – a Pleistocene beach deposit from Cape Mondego (Figueira da Foz, West Central Portugal)*

Among the Pleistocene and Holocene units recorded near the marine cliffs of Cape Mondego (Figueira da Foz, West Central Portugal) stands out the Farol Deposit (Depósito do Farol), at an altitude of ±95 m above present sea level. It is a marine terrace with three exposures of interstratified conglomerates and sands, overlapped by calclititic-fanglomerates. This sedimentary setting indicates that deposition took place in a seashore environment influenced by the proximity of a marine palaeocliff. The deposit has an interesting subfossil fauna with abraded and fragmented shells of Nucella lapillus (LINNÉ, 1758), Patella vulgata (LINNÉ, 1758) and Littorina littorea (LINNÉ, 1758), suggesting the existence of an environment with colder surface seawater, when compared with the present day Portuguese seashore. These specimens belonged to marine communities adapted to live in intertidal rocky platforms, which have been exposed to the cyclic action of waves and tidal flows, on the swash and surf zones. The Farol Deposit can be related to an Early/Middle Pleistocene “cold-water” episode, earlier to the Isotopic Stages 7 and 11. This episode occurred before the deposition of the units Quiaios Sands (Areias de Quiaios) and Cantanhede Sands (Areias de Cantanhede) (Sicilian?), but later than the Arazede Sands (Areias de Arazede) and Marinha das Ondas Sands (Areias de Marinha das Ondas) (Early Pleistocene).

Monitoring the ex-vivo expansion of human mesenchymal stem/stromal cells in xeno-free microcarrier-based reactor systems by MIR spectroscopy

Human mesenchymal stem/stromal cells (MSCs) have received considerable attention in the field of cell-based therapies due to their high differentiation potential and ability to modulate immune responses. However, since these cells can only be isolated in very low quantities, successful realization of these therapies requires MSCs ex-vivo expansion to achieve relevant cell doses. The metabolic activity is one of the parameters often monitored during MSCs cultivation by using expensive multi-analytical methods, some of them time-consuming. The present work evaluates the use of mid-infrared (MIR) spectroscopy, through rapid and economic high-throughput analyses associated to multivariate data analysis, to monitor three different MSCs cultivation runs conducted in spinner flasks, under xeno-free culture conditions, which differ in the type of microcarriers used and the culture feeding strategy applied. After evaluating diverse spectral preprocessing techniques, the optimized partial least square (PLS) regression models based on the MIR spectra to estimate the glucose, lactate and ammonia concentrations yielded high coefficients of determination (R2 ≥ 0.98, ≥0.98, and ≥0.94, respectively) and low prediction errors (RMSECV ≤ 4.7%, ≤4.4% and ≤5.7%, respectively). Besides PLS models valid for specific expansion protocols, a robust model simultaneously valid for the three processes was also built for predicting glucose, lactate and ammonia, yielding a R2 of 0.95, 0.97 and 0.86, and a RMSECV of 0.33, 0.57, and 0.09 mM, respectively. Therefore, MIR spectroscopy combined with multivariate data analysis represents a promising tool for both optimization and control of MSCs expansion processes.

Microencapsulation technology: a powerful tool for human embryonic stem cells expansion and cryopreservation

Dissertation presented to obtain a Master degree in Biotechnology at the Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Impact of Distributed Generation in the Transmission System Expansion Planning

In this work, the impact of distributed generation in the transmission expansion planning will be simulated through the performance of an optimization process for three different scenarios: the first without distributed generation, the second with distributed generation equivalent to 1% of the load, and the third with 5% of distributed generation. For modeling the expanding problem the load flow linearized method using genetic algorithms for optimization has been chosen. The test circuit used is a simplification of the south eastern Brazilian electricity system with 46 buses.

Bridging environmental conflicts with social metabolism : forestry expansion and socioeconomic change

Dissertação apresentada para obtenção do Grau de Doutor em Ciências do Ambiente, pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Distribution of hepatitis C virus genotypes among blood donors from mid-west region of Brazil

In order to investigate the hepatitis C virus (HCV) genotypes in mid-west region of Brazil, 250 anti-HCV positive blood donors were studied. Among them, the anti-HCV serological status was confirmed in 205 (82%). HCV RNA was detected in 165 samples, which were genotyped. HCV types 1, 2 and 3 were found in 67.9%, 3% and 29.1% of the donors, respectively. In Goiás state, subtype 1a (50%) was the most prevalent, followed by subtypes 3a (30.9%) and 1b (16.7%). In Mato Grosso state, subtype 1a was also predominant (41%), followed by subtypes 1b (29.5%) and 3a (25%). In Mato Grosso do Sul state, subtypes 1a and 1b were detected equally (36.8%), followed by 3a (21.1%). Subtype 2b was rare (2.4%, 4.5% and 5.3%, respectively). In Distrito Federal, subtype 3a (39%) was more frequent than 1a (31.7%) and the remaining (29.3%) belonged to subtype 1b.

EXPANSION OF VISCERAL LEISHMANIASIS IN THE STATE OF RIO DE JANEIRO, BRAZIL: REPORT OF THE FIRST AUTOCHTHONOUS CASE IN THE MUNICIPALITY OF VOLTA REDONDA AND THE DIFFICULTY OF DIAGNOSIS

Visceral Leishmaniasis has been showing remarkable epidemiological changes in recent decades, with marked expansion and an emergence of cases in urban areas of the North, Southeast and Midwest regions of Brazil. The Kala-azar cases reported here, despite being very characteristic, presented a great difficulty of diagnosis, because the disease is not endemic in Volta Redonda. The child underwent two hospitalizations in different hospitals, but got the correct diagnosis only after 11 months of symptom onset. In this report we discuss the main differential diagnoses and call attention to the suspected symptoms of visceral leishmaniasis in patients with prolonged fever, hepatosplenomegaly and pancytopenia, even in areas not traditionally endemic for the disease.