Food Colours: Existing and Emerging Food Safety Concerns

Food colours are added to different types of commodities to increase their visual attractiveness or to compensate for natural colour variations. The use of these additives is strictly regulated in the European Union, the United States and many other countries worldwide. There is a growing concern about the safety of some commonly used legal food colourants and there is a trend to replace the synthetic forms with natural products. Additionally, a number of dyes with known or suspected genotoxic or carcinogenic properties have been shown to be added illegally to foods. Robust monitoring programs based on reliable detection methods are required to assure the food is free from harmful colours. The aim of this review is to present an up to date status of the various concerns arising from use of colour additives in food. The most important food safety concerns in the field of food colours are lack of uniform regulation concerning legal food colours worldwide, possible link of artificial colours to hyperactive behaviour, replacement of synthetic colours with natural ones and the presence of harmful illegal dyes - both known but also new, emerging ones in food. The legal status of food colour additives in the EU, US and worldwide is summarized. The reported negative health effects of both legal and illegal colours are presented. The European Rapid Alert System for Food and Feed notifications and US import alerts concerning food colours are analyzed and trends in fraudulent use of colour additives identified. The detection methods for synthetic colours are also reviewed.

Industrial Organization: a Contract Based approach

Industrial Organization, a Contract Based approach (aka IOCB) offers an extensive and an up-to-date panorama of Industrial Organization. It is aimed at advanced undergraduates, graduates, academics and practitioners with an interest in the field. The analysis of market interactions, business strategies and public policy is performed using the standard framework of game theory and the recent advances of contract theory and information economics

Estructura computacional i aplicacions de la semblança molecular quàntica

La tesis tracta diferents aspectes relacionats amb el càlcul de la semblança quàntica, així com la seva aplicació en la racionalització i predicció de l'activitat de fàrmacs. Es poden destacar dos progressos importants en el desenvolupament de noves metodologies que faciliten el càlcul de les mesures de semblança quàntica. En primer lloc, la descripció de les molècules mitjançant les funciones densitat aproximades PASA (Promolecular Atomic Shell Approximation) ha permès descriure amb suficient precisió la densitat electrònica dels sistemes moleculars analitzats, reduint substancialment el temps de càlcul de les mesures de semblança. En segon lloc, el desenvolupament de tècniques de superposició molecular específiques de les mesures de semblança quàntica ha permès resoldre el problema de l'alineament en l'espai dels compostos comparats. El perfeccionament d'aquests nous procediments i algoritmes matemàtics associats a les mesures de semblança molecular quàntica, ha estat essencial per poder progressar en diferents disciplines de la química computacional, sobretot les relacionades amb les anàlisis quantitatives entre les estructures moleculars i les seves activitats biològiques, conegudes amb les sigles angleses QSAR (Quantitative Structure-Activity Relationships). Precisament en l'àrea de les relacions estructura-activitat s'han presentat dues aproximacions fonamentades en la semblança molecular quàntica que s'originen a partir de dues representacions diferents de les molècules. La primera descripció considera la densitat electrònica global de les molècules i és important, entre altres, la disposició dels objectes comparats en l'espai i la seva conformació tridimensional. El resultat és una matriu de semblança amb les mesures de semblança de tots els parells de compostos que formen el conjunt estudiat. La segona descripció es fonamenta en la partició de la densitat global de les molècules en fragments. S'utilitzen mesures d'autosemblança per analitzar els requeriments bàsics d'una determinada activitat des del punt de vista de la semblança quàntica. El procés permet la detecció de les regions moleculars que són responsables d'una alta resposta biològica. Això permet obtenir un patró amb les regions actives que és d'evident interès per als propòsits del disseny de fàrmacs. En definitiva, s'ha comprovat que mitjançant la simulació i manipulació informàtica de les molècules en tres dimensions es pot obtenir una informació essencial en l'estudi de la interacció entre els fàrmacs i els seus receptors macromoleculars.

Clave del relato histórico de Alfredo Pareja Diezcanseco: herencia colonial, Revolución liberal y mestizaje (Del Historiador y el Ensayista)

El autor analiza la perspectiva de Pareja sobre los problemas y logros del país hasta los años cincuenta, argumenta que es la de un republicano laico, socialista, modernizador y guayaquileño. Algunas valoraciones de Pareja se inscriben en el contexto de la inexistencia de un campo de saber historiográfico constituido en el Ecuador de esos años. Su perspectiva de la conquista tiene elementos de una visión hispanista del devenir histórico, que quiere reconciliarse con la búsqueda de una modernidad no alcanzada y afanosamente deseada. Respecto de la independencia, la libertad no se consagró en 1822, sino recién en 1895, con la Revolución liberal, que llevaría a la modernización del Estado, en ella jugaría un rol central el mestizo como actor colectivo. Finalmente, Pareja atribuye al mestizaje la posibilidad de incorporación del país a la dinámica civilizadora, de puesta al día con los valores de la democracia y el progreso.

Análise crítica dos produtos de mobilidade sentada - cadeiras de rodas - utilizados por crianças e adolescentes com paralisia cerebral em Natal/RN e outros municípios do Rio Grande do Norte

The objective of this work is to critically analyze the seated mobility adaptive equipment, wheelchair, used by non-ambulatory children/adolescents diagnosed with cerebral palsy of the quadriplegy type with ages in between 0-18 years old in the municipal city of Natal/RN and of other ones in the state of Rio Grande do Norte. This study is characterized as descriptive as it took into consideration the individualized conditions of each child/adolescent and of their home environment, and also evaluates the condition of the used seated mobility devices such as wheelchairs or strollers. Thirty three (33) subjects which had a wheelchair prescribed by a healthcare professional specialist in adaptive equipment within the past 3 years were selected. A questionnaire was applied through a phone with the patient s primary care giver or parents. The results obtained showed that all the equipment being used had an anatomical seat and back and the tilt system mechanism and even though most of the patients required small individual adjustments upon receiving the wheelchair in order to accommodate to the patients characteristics. From the point of view of the caregiver/parents interviewed, 97% described satisfied with the equipment and report assistance with mobility (97%), comfort (82%) and good posture (82%) as the main positive aspects from the equipment. The results also describe the aspects related to the maintenance, time used and activities developed that were reported by the patients. The conclusion therefore is that in order to select the equipment to meet the needs of the consumer it is necessary that: an appropriate wheelchair prescription takes place, the up-to-date knowledge of equipment available in the market, the appropriate use of the resources from the concession programs of the government that purchase equipment and the establishment of link in between the manufacturers, patients and the caregivers

Macroeconomic Report on Latin America and the Caribbean. June 2012

Incluye Bibliografía

Influencia de la separación de residuos sólidos urbanos para reciclaje en el proceso de incineración con generación de energía

This work analyses the recuperation of the energy of Municipal Solid Waste (MSW) through the incineration process. It considers the up to date tendency of segregation (separation) of plastic, paper and cardboard, glass and metals and their influence in the fluxes of mass and energy in the incineration system of MSW. For its development was used information related to the generation of MSW in Bauru city and the Combust software. The results so obtained allowed the estimation of the Caloric power of the typical MSW and also of this residue when one considers the separations of paper/cardboard and plastic for recycling.

Job-seeking strategies in Trinidad and Tobago

Includes bibliography

Leitura e escrita em fonoaudiologia: a transição de paradigmas

Pós-graduação em Educação - FFC

Ocupação, políticas públicas e gestão ambiental de unidade de conservação do Estado de Rondônia: o estudo de caso do Parque Estadual de Guajará Mirim

Pós-graduação em Geociências e Meio Ambiente - IGCE

Histórias de pescadores: memórias de vidas submersas

Pós-graduação em Psicologia - FCLAS

O livro que divulgou o papiro Rhind no Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Evidence for dark matter in the inner Milky Way

The ubiquitous presence of dark matter in the Universe is today a central tenet in modern cosmology and astrophysics(1). Throughout the Universe, the evidence for dark matter is compelling in dwarfs, spiral galaxies, galaxy clusters as well as at cosmological scales. However, it has been historically difficult to pin down the dark matter contribution to the total mass density in the Milky Way, particularly in the innermost regions of the Galaxy and in the solar neighbourhood(2). Here we present an up-to-date compilation of Milky Way rotation curve measurements(3-13), and compare it with state-of-the-art baryonic mass distribution models(14-26). We show that current data strongly disfavour baryons as the sole contribution to the Galactic mass budget, even inside the solar circle. Our findings demonstrate the existence of dark matter in the inner Galaxy without making any assumptions about its distribution. We anticipate that this result will compel new model-independent constraints on the dark matter local density and profile, thus reducing uncertainties on direct and indirect dark matter searches, and will help reveal the structure and evolution of the Galaxy.

Reinigung und Coexpression von Enzymen der Raubasin-Biosynthese

Die vorgestellten Arbeiten bezüglich der Biosynthese von pflanzlichen Indolalkaloiden können in einen molekularbiologischen und einen proteinche-mischen Teil aufgegliedert werden. Im molekularbiologischen Abschnitt stand die Entwicklung eines Vektorsystems im Vordergrund, das die gleichzeitige Expression mehrerer Enzyme in bakteriellen Kulturen erlaubte. Hierfür konnte zunächst die cDNA der Strictosidin-Synthase aus Rauvolfia serpentina in den Expressionsvektor pQE-70 einkloniert und das Protein aktiv exprimiert werden. Bevor es zum Einbau des Strictosidin-β-D-Glucosidase-Gens –ebenfalls aus Rauvolfia serpentina– kam, musste dessen Aktivität mit einer vorgeschalteten Ribosomenbindestelle sichergestellt werden. Diese zusätzliche Binderegion wurde an das 5’-Ende der cDNA angefügt, um die ungestörte Expression des Enzyms im späteren Coexpressions-System zu gewährleisten. Im Hinblick auf weiterführende Arbeiten in Bezug auf die komplette in vitro-Synthese bereits bekannter Alkaloide, wie z.B. das antiarrhythmisch wirkende Ajmalin oder das antihypertensiv wirkende Heteroyohimbin-Alkaloid Raubasin, wurde die ursprüngliche multiple-clonig-site des verwendeten Expressionsvektors pQE-70 um 27 zusätzliche Restriktionsschnittstellen (verteilt auf 253 bp) erweitert. Nach erfolgreicher Ligation der Strictosidin-β-D-Glucosidase-cDNA mit vorgeschalteter Ribosomenbindestelle an das 3’-Ende des Strictosidin-Synthase-Gens gelang die heterologe Coexpression beider Enzyme in einer homogenen Suspensionskultur des E. coli-Expressionsstamms M15. Dafür wurde das Vektorkonstrukt pQE-70bh-STR-RBS-SG entwickelt. Das Endprodukt der anschließenden enzymatischen Umsetzung von Tryptamin und Secologanin wurde über das Zwischenrodukt Strictosidin gebildet und konnte als Cathenamin identifiziert werden. Im proteinchemischen Teil der Dissertation wurde die Reinigung einer Cathenamin-Reduktase aus Zellsuspensionskulturen von Catharanthus roseus RC mit dem Ziel der partiellen Bestimmung der Aminosäuresequenz bearbeitet. Das gesuchte Enzym wandelte in einer NADPH-abhängigen Reaktion das Edukt Cathenamin in Raubasin um. Des weiteren wurde untersucht, wie viele Enzyme insgesamt an der Umwandlung von Cathenamin zu Raubasin und den eng verwandten Produkten Tetrahydroalstonin und 19-Epi-Raubasin beteiligt waren. Unter Anwendung eines hierfür entwickelten säulenchromatographischen Protokolls gelang die Reinigung einer Raubasin-bildenden Reduktase, deren Teilsequenz jedoch noch nicht bestimmt werden konnte. Die Anzahl der beteiligten Enzyme bei der Ausbildung von Raubasin, Tetrahydroalstonin und 19-Epi-Raubasin konnte auf mindestens zwei beziffert werden.

Analisi climatica ad alta risoluzione delle precipitazioni sul nord Italia (1961-2005)

L’obiettivo di questo lavoro di tesi è di ottenere un’analisi climatica giornaliera ad alta risoluzione della precipitazione sul territorio del nord Italia realizzata con tecniche di controllo statistico, di analisi e di strumenti di descrizione dei risultati presentati nella recente letteratura. A tal fine, sono stati utilizzati i dati dell’Archivio ARCIS. In seguito alle fasi di controllo qualità, omogeneità e sincronicità i dati sono stati utilizzati per realizzare un’analisi giornaliera su grigliato regolare a 10 km di risoluzione utile alla rappresentazione della variabilità spazio-temporale della precipitazione sul Nord Italia per il periodo 1961-2005. I risultati di tale analisi mettono in evidenza dei valori medi di precipitazione annuale abbastanza intensi sulla parte centrale dell’arco Alpino, con massimi (oltre 2000 mm) sull’estremità orientale e sull’Appennino Ligure. Valori minimi (500 – 600 mm) sono osservati lungo le aree prospicienti il fiume Po, in Val d’Aosta ed in Alto Adige. La corrispondente analisi del trend temporale indica la presenza di lievi cali statisticamente significativi solo in aree limitate del territorio. In coerenza con questi risultati, la variazione nel tempo della precipitazione annuale mediata su tutto il territorio mette in evidenza un’intensa variabilità decennale, ma solo una lieve flessione lineare sull’intero periodo. Il numero annuo di giorni piovosi ed il 90° percentile della precipitazione giornaliera presentano invece trend lineari un po’ più pronunciati. In particolare, sul periodo considerato si nota un calo del numero di giorni piovosi su gran parte del territorio e solo su alcune aree del territorio un aumento dell’intensità del 90° percentile, sia a scala annuale che stagionale. Nell’ultima parte di questo lavoro è stato realizzato uno studio della relazione fra la forzante climatica e l’evoluzione della morfologia dell’Appennino Emiliano-Romagnolo. I risultati mostrano che a parità di quota, di pendenza e di litologia, la franosità è influenzata dalle precipitazioni.