Diferencia����o miog��nica ���in vitro��� de c��lulas tronco mesenquimais murinas de diferentes origens

Muscular dystrophy refers to a group of more than 30 genetical disorders characterized by progressive weakness and degeneration of the skeletal muscle. No effective therapy is available at present. Recent studies have reported that the transplantation of stem cells can offer an important potential therapy for genetic diseases. Adult bone marrow mesenchymal stem cells have been identified as a nonhematopoietic stem cell population capable of self-renewal with the ability to differentiate into many cell lineages, including bone, fat, cartilage and connective tissue. Because of their similarity with muscle progenitor cells, when they are injected in affected individuals, they are able to migrate into areas of skeletal muscle degeneration and participate in the regeneration process. The adipose tissue represents an alternative source of MSCs that, as the MSCs derived from bone marrow, are capable of in vitro differentiation into osteogenic, adipogenic, myogenic and chondrogenic lineages. The objective of this project is to investigate the ���in vitro��� myogenic potential of mesenchymal stem cells derived from murine bone marrow and adipose tissue. Four experimental groups were analyzed: mice from lineages Lama2dy-2J/J and C57black and, C2C12 lineage cells and transformed C2C12 expressing the eGFP protein. MSCs cultures were obtained by flushing the bone marrow femurs and tibials with ��-MEM or by the subcutaneous and inguinal fat from the mice. Their characterization was done by flow cytometry and in vitro differentiation. Muscle differentiation was studied through the analysis of the expression of transcriptional factors involved in muscle differentiation and/or the presence and amount of specific proteins from muscle differentiated cell. The pluripotency from bone marrow MSCs of the two lineages was evidenced and, in the muscular differentiation... (Complete abstract click electronic access below)

Influ��ncia de diferentes extratos de Cordia ecalyculata em cultura de c��lulas-tronco mesenquimais humanas

Diante da constata����o cl��nica de que muitos pacientes n��o conseguem benef��cio completo da remiss��o da ulcera����o em membros inferiores com os tratamentos convencionais, o Setor de Biotecnologia do Hemocentro de Botucatu estabeleceu o protocolo de produ����o de curativos bioativos, que interagem com a les��o e estimulam via horm��nios de crescimento (PDGF, VEFG, FGF e outros) a recupera����o da ��rea danificada por indu����o das c��lulas-tronco comissionadas da pele. Estudos realizados na Faculdade de Qu��mica de Araraquara culminaram com a purifica����o do princ��pio ativo do fitoter��pico Cordia ecalyculata para analisar in vitro o seu alto potencial antiinflamat��rio. Seus diferentes extratos foram enviados ao Laborat��rio de Engenharia Celular da Faculdade de Medicina de Botucatu: extrato bruto (EB), fase aquosa (FA), fase hex��nica (FH) e fase et��rea (FE), que foram testados em diferentes concentra����es em cultura de c��lulas-tronco mesenquimais humanas (CTM), oriundas de tecido adiposo, com a finalidade de analisar, atrav��s de caspase-3, marcador intracelular, o ��ndice de apoptose. Aquele que apresentar o melhor resultado ser�� adicionado �� f��rmula dos biocurativos. Este trabalho tem os objetivos de avaliar a contribui����o da cultura celular de CTM nos testes de toler��ncia com Cordia ecalyculata, estabelecer o ��ndice de apoptose celular e avaliar o tempo de conflu��ncia maior que 80% na cultura de CTM em diferentes concentra����es de C. ecalyculata. Os resultados encontrados comprovaram que os extratos que apresentaram os melhores desempenhos foram os da FA em todas as concentra����es e FE de 25��g/mL. Os extratos FE nas demais concentra����es demonstraram um desempenho insatisfat��rio,o que nos indica que uma concentra����o entre 25 e 50��g/mL seja o limite entre a boa execu����o nas c��lulas e toxicidade. Mais testes ter��o que ser realizados para demonstrar qual �� essa concentra����o de FA �� realmente melhor a ser utilizada

Fatores indutores e supressores da eritropoiese em caninos doentes renais cr��nicos

P��s-gradua����o em Cirurgia Veterin��ria - FCAV

Avalia����o da sensibilidade insul��nica, transdu����o do sinal insul��nico e da densidade mineral ��ssea em ratas tratadas com fluoreto de s��dio

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Condi����o corporal e jejum sobre o metabolismo energ��tico e crescimento de juvenis de pacu

P��s-gradua����o em Zootecnia - FCAV

Efeito dos h��bitos comportamentais e gordura corporal sobre indicadores de risco cardiovasculares entre adolescentes: estudo de coorte de 12 meses

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Express��o das prote��nas envolvidas no tr��nsito de c��lcio mioc��rdico de ratos obesos por dieta hiperlip��dica saturada

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Estudo comparativo de citocinas anti-inflamat��rias em c��lulas tronco mesenquimais cultivadas com Cordia ecalyculata e biocurativo

P��s-gradua����o em Pesquisa e Desenvolvimento (Biotecnologia M��dica) - FMB

Cell therapy in experimental model of inflammatory bowel disease

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Matrizes de quitosina-col��geno podem regular as atividades biol��gicas de c��lulas tronco mensenquimais adiposas para a engenharia de tecidos

P��s-gradua����o em Biof��sica Molecular - IBILCE

Influ��ncia da obesidade induzida por dieta hiperlip��dica saturada sobre o comportamento da via beta-adren��rgica mioc��rdica em ratos Wistar

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury

Cell therapy has frequently been reported as a possible treatment for spinal trauma in humans and animals; however, without pharmacologically curative action on damage from the primary lesion. In this study, we evaluated the effect of administering human adipose-derived stem cells (hADSC) in rats after spinal cord injury. The hADSC were used between the third and fifth passages and a proportion of cells were transduced for screening in vivo after transplantation. Spinal cord injury was induced with a Fogarty catheter no. 3 inserted into the epidural space with a cuff located at T8 and filled with 80 mu L saline for 5 min. The control group A (n = 12) received culture medium (50 mu L) and group B (n = 12) received hADSC (1.2 x 10(6)) at 7 and 14 days post-injury, in the tail vein. Emptying of the bladder by massage was performed daily for 3 months. Evaluation of functional motor activity was performed daily until 3 months post-injury using the Basso-Beattie-Bresnahan scale. Subsequently, the animals were euthanized and histological analysis of the urinary bladder and spinal cord was performed. Bioluminescence analysis revealed hADSC at the application site and lungs. There was improvement of urinary bladder function in 83.3% animals in group B and 16.66% animals in group A. The analysis of functional motor activity and histology of the spinal cord and urinary bladder demonstrated no significant difference between groups A and B. The results indicate that transplanted hADSC improved urinary function via a telecrine mechanism, namely action at a distance.

Rela����o entre a ingest��o de micronutrientes, perfil de citocinas plasm��ticas e express��o dos genes IL-6. IL-10 e TNF-�� na obesidade m��rbida

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Resposta da resist��ncia insul��nica de mulheres menopausadas ao protocolo de exerc��cio intervalado em esteira ergom��trica

P��s-gradua����o em Ginecologia, Obstetr��cia e Mastologia - FMB

Erva mate minimiza as altera����es do perfil lip��dico promovidas por elevado consumo de sacarose

The yerba mate (Ilex paraguariensis) is composed of bioactive that interfere with lipid metabolism. The objective was to evaluate if daily consumption of mate tea (MT) change the lipid deposits and dyslipidemia caused by excessive consumption of sucrose. Thirty male Wistar rats (40 days old) were divided into four groups: Group C - free access to commercial chow and deionized water; S - free access to commercial food, water and sucrose solution 30% (w/v) in water; MTS and deionized - free access to commercial feed solution, water, 30% sucrose (w/v) and treated with daily infusion of MT (soluble mate Le��o J��nior��) via orogastric tube at a dose of 100 mg/kg/mc for 8 weeks. After the trial period the lipid profile was evaluated by the following parameters: a) direct weighing of the retroperitoneal (RP) and epididymal (EPI) adipose tissues; b) determination of plasma total cholesterol, triglycerides and HDL cholesterol concentration. The MT promoted the reduction of 1.4 times in both tissues RP and EPI in MTS group compared to group S. Treatment with MT decreased 2.7 times triglyceride in the MTS group compared with the group S. The sucrose consumption did not alter the plasma cholesterol concentration, however the consumption of MT significantly reduced total cholesterol circulating. The HDL cholesterol concentration, in the MTS group, showed higher concentration than in group S (1.3 times). MT prevents in young male rats the increase of lipid deposits and dyslipidemia caused by excessive consumption of sucrose.