Membrane fractionation technologies for high quality mill sugar and value-added by products - an integrated sugar production process concept model.

Membrane filtration technology has been proven to be a technically sound process to improve the quality of clarified cane juice and subsequently to increase the productivity of crystallisation and the quality of sugar production. However, commercial applications have been hindered because the benefits to crystallisation and sugar quality have not outweighed the increased processing costs associated with membrane applications. An 'Integrated Sugar Production Process (ISPP) Concept Model' is proposed to recover more value from the non-sucrose streams generated by membrane processing. Pilot scale membrane fractionation trials confirmed the technical feasibility of separating high-molecular weight, antioxidant and reducing sugar fractions from cane juice in forms suitable for value recovery. It was also found that up to 40% of potassium salts from the juice can be removed by membrane application while removing the similar amount of water with potential energy saving in subsequent evaporation. Application of ISPP would allow sugar industry to co-produce multiple products and high quality mill sugar while eliminating energy intensive refining processes.

Production of 2-phenethyl alcohol and 2-phenyllactic acid in Candida species

2-Phenethyl alcohol (2-PEA) and 2-phenyllactic acid (2-PLA) were isolated from the culture filtrates of Candida species grown in media containing peptone or phenylalanine as nitrogen source. These compounds were characterized by comparing their UV, IR, and NMR spectral properties with authentic samples. Candida species differed markedly in their production of 2-PEA and 2-PLA. Experiments using [14C]-phenylalanine indicated that both 2-PEA and 2-PLA are synthesised from L-phenylalanine. A pathway for the biosynthesis of 2-PEA from L-phenylalanine has been proposed.

An NAD(P)(+)-dependent secondary-alcohol dehydrogenase of Alcaligenes eutrophus: Purification, characterization and its application for the production of chiral alcohols

A soil micro-organism identified as Alcaligenes eutrophus capable of utilizing nerolidol, a sesquiterpene alcohol as the sole source of carbon, contains an inducible NAD(P)(+)-linked secondary-alcohol dehydrogenase (SADH), The enzyme was purified 252-fold from crude cell-free extract by a combination of salt precipitation, ion-exchange and affinity-matrix chromatography, Native and SDS/PAGE PAGE of the purified enzyme showed a single protein band and the enzyme appears to be a homotetramer having an apparent molecular mass of 139 kDa comprising four identical subunits of 38.5 kDa, The isoelectric point (pi) of SADH was determined to be 6.2, Depending on pH of the reaction media, the enzyme carried out both oxidation and reductions of various terpenoids and steroids, At pH 5.5, the enzyme catalysed the stereospecific reduction of prochiral ketones to optically active (S)-alcohols and the oxidation reaction was predominated over the former at pH 9.5, NADP(+) and NADPH were respectively preferred over NAD(+) and NADH for oxidation and reduction reactions, The K-m values for testosterone, NADP(+) and NAD(+) were 11.8, 55.6, and 122 mu M respectively, Neither enzyme was significantly inhibited by metal-binding agents, but some thiol-blocking compounds inhibited it, SADH tolerates moderate concentrations of water-miscible organic solvents such as ethanol, methanol, acetone and dioxan, Some of the properties of this enzyme were found to be significantly different from those thus far described.

Climate change impacts on the water services in Costa Rica: a production function for the hydroenergy sector

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Factors influencing colour production from the sugar medium used for the rapid approximation of bacterial counts in fishery products

Results of the studies carried out to elucidate the factors influencing colour production from the sugar medium used for the rapid approximation of bacterial counts in fishery products are reported. The effect of particle size, trace elements, salt soluble protein and non-protein fractions, rate of multiplication of bacteria, in the medium, surface bacteria and the rate of colour production by individual strains of bacteria were studied. It is observed that the best results are obtained when a sea-water homogenate is used.

Feasibility study of biofuel production from freshwater fern, Azolla sp in Anzali Wetland

Production of bioethanol through acidic and enzymatic hydrolysis of aquatic Azolla sp., as a new source of bio-mass, has been performed, as a means to control increasing growth and reducing undesirable effects of this plant in Anzali lagoon. After sampling, drying and crushing, Azolla was hydrolyzed, using diluted acid and enzyme. Diluted acid hydrolysis was done using both autoclave and a high-pressure system (Batch Synth® Microwave synthesizer). The effects of temperature and time (in autoclave) and concentration of acid (in both) were compared. Cellubrix®, a ommercial cellulase source, was used for enzymatic hydrolysis process. The amounts of reducing sugars, glucose and furfural, released from hydrolyzate, were measured. To produce alcohol, Sacchromyces cerevisiae (to ferment sixcarbon sugars), Zygowilliopsis californica and Pichia stipitis (to ferment five-carbon and sixcarbon sugars) were used. Maximum amounts of glucose (4.83% w/w) and reducing sugars (14.15% w/w) were obtained using acid hydrolysis in autoclave. In the microwave oven, maximum glucose (5.04% w/w) and reducing sugars (13.27 w/w) were obtained at 180 and 200 °C, respectively. Under these conditions, maximum produced furfural was 1.54 g/L. The difference between amounts of furfural obtained from acid hydrolysis of Azolla in microwave oven compared to autoclave was statistically significant. Amounts of alcohol produced and its yields were 3.99 g/L and 33.13% for S. cerevisiae in 48 hours, 3.73 g/L and 30.45% for Pichia stipites in 48 hours, and 3.73 g/L and 30.45% for Z. californica in 24 hours after inoculation, respectively, with significant differences. Statistical comparison of results showed significant differences (P<0.05) in glucose production, at different conditions. Amounts of reducing sugars and glucose increased after optimization of levels of acid, time, and temperature. The overall optimum released sugar and glucose were obtained with 1.67% (w/v) acid using autoclave. Higher temperatures in microwave oven caused a significant increase (P<0.05) in furfural. Furfural severely inhibits fermentation. Hence, regarding the issues of energy consumption and time, amounts of inhibiting substances and sugar production, autoclave is found to be superior to the high temperature and pressure, generated in microwave oven, for hydrolyzing Azolla. Furthermore, given the amounts of Azolla in Anzali lagoon, it may be recommendable to use this plant as a biomass resource.

Functional food production from the prymary sector : prospects and opportunities

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Functional food production from the prymary sector : prospects and opportunities

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Phase equilibria modeling for biofuels production

Em consequência de uma série de problemas ambientais, económicos e políticos relacionados com o uso de combustíveis convencionais, vários países estão agora a focar as suas atenções em combustíveis alternativos. O biodiesel está na linha da frente das alternativas ao petróleo no sector dos transportes, sendo considerado uma opção a curto prazo visto que o seu preço é competitivo e não são necessárias mudanças nos motores para implementar o seu uso. De entres os possíveis processos de produzir biodiesel, a reacção de transesterificação com catálise básica é o método preferido. Depois da reacção são sempre necessários processos de purificação de modo ao biodiesel produzido cumprir os standards definidos para os combustíveis alternativos, reduzindo problemas de motor e consequentemente aumentando a sua aceitação por parte dos consumidores. De entre as especificações encontram-se o conteúdo em água, em álcool e em glicerol. Ser-se capaz de descrever correctamente o equilíbrio de fases de sistemas que são de interesse para os processos de purificação de biodieseis numa gama alargada de condições termodinâmicas é uma condição necessária para uma correcta simulação do processo industrial, de modo a se atingir uma elevada produtividade a baixos custos de operação. O uso de moléculas oxigenadas como combustíveis representa uma alteração significativa em termos da termodinâmica de soluções. Para combustíveis baseados em petróleo as equações de estado cúbicas e os modelos clássicos de coeficientes de actividade mostraram ser apropriados, no entanto para combustíveis novos como o biodiesel, sendo mais complexos do ponto de vista das interacções intermoleculares com formação de dipolos e pontes de hidrogénio, são necessários modelos termodinâmicos mais complexos para descrever essas interacções. Neste trabalho a CPA EoS (Cubic-Plus-Association Equation of State) será desenvolvida de modo a permitir uma descrição adequada dos equilíbrios líquido-vapor e líquido-líquido para uma serie de sistemas binários e multicomponentes contendo água, ácidos gordos, ésteres de ácidos gordos, glicerol e álcoois.

Advances on the production of polyhydroxyalkanoates (PHA) by mixed microbial cultures from sugar cane molasses

Dissertação para obtenção do Grau de Mestre em Biotecnologia

Diseño de un plan de mejoramiento para el sector panelero en el municipio de Santana - Boyacá

El siguiente trabajo de grado se desarrolla para el sector panelero en el municipio de Santana – Boyacá, ubicado en la Hoya del Río Suárez, lugar en donde la producción de panela constituye la actividad económica más importante de la región. El objetivo del trabajo es la formulación de una estrategia que permita el fortalecimiento de dicho sector, de tal manera que se pueda mejorar la situación actual del mismo. A lo largo del estudio se analizan diferentes factores que recaen en la situación actual del sector; después de utilizar las herramientas del Modelo Matricial y realizar diversas encuestas a paneleros y consumidores, se plantea y evalúa la alternativa que conlleve a la implementación de formas de cooperativismo como solución. Se define el cooperativismo como oportunidad de mejora, ya que por medio del trabajo en equipo y la consolidación de esfuerzos se conseguirían grandes beneficios para el sector; tales como: reconocimiento de marca, mejores condiciones laborales, estabilidad en precios y grandes oportunidades de innovación. La solución propuesta de unificar productores paneleros se compone del establecimiento de un fondo panelero, encargado de mantener precios estándar para los productores y protegerlos de la volatilidad que los caracteriza. La cooperativa organizaría a los productores, además de asegurar la comercialización e incentivar el consumo de panela en el municipio de Santana-Boyacá.

Un panorama de la productividad del sector azucarero colombiano

El presente trabajo se basa en la investigación y análisis del sector azucarero, para presentar un panorama de la productividad del sector en Colombia. Para ello se utilizan tres países de referencia, Australia, Argentina y Guatemala, con los cuales se hace una comparación detallada a lo largo del trabajo. Éste trabajo consta de cinco partes. En la primera parte se realizará una introducción del sector azucarero en Colombia en comparación con otros países con diferentes niveles de producción de azúcar, y dos posibles indicadores para la medición de la productividad del sector en Colombia. En la segunda parte se planteará y describirá el problema de investigación junto con la pregunta a desarrollar a lo largo del trabajo. En la tercera parte se construirá un estado del arte, para así tomar como referencia otros estudios realizados sobre el tema. En la cuarta parte del trabajo se plantearán las posibles soluciones al problema de investigación. Y finalmente, en la quinta parte del trabajo se darán recomendaciones y se sacarán las conclusiones de todo el estudio. Teniendo como objetivo el análisis de la productividad del sector, se tomaran como base dos indicadores de productividad. El primero de ellos será el indicador de productividad laboral, el cual definirá el aumento o disminución de los rendimientos ocasionados por la variación de cualquiera de los factores que intervienen en la producción (trabajo, capital o tecnología) . Para medir este indicador se utiliza también el índice de Aporte Laboral (valor agregado /personal total), el cual muestra la gestión de los trabajadores mostrando la productividad media del trabajo. El segundo indicador que se analizará está medido por factores tales como: toneladas de azúcar, toneladas de caña y hectáreas por cosecha. Este indicador se podrá medir por medio del TAH-Cosecha (Tonelada de azúcar/hectárea) y el TCH-Cosecha (Tonelada de caña/hectárea) . Por medio de la construcción y análisis de estos indicadores se desea ver el impacto y relación de la productividad del sector azucarero en Colombia. A partir de este estudio se pretende brindar sugerencias para mejorar la productividad del sector azucarero colombiano, siendo este comparado con las prácticas y productividades de Argentina, Australia y Guatemala.