Influence of elastase on alanine-rich peptide hydrogels

The self-assembly of the alanine-rich amphiphilic peptides Lys(Ala)6Lys (KA6K) and Lys(Ala)6Glu (KA6E)with homotelechelic or heterotelechelic charged termini respectively has been investigated in aqueous solution. These peptides contain hexa-alanine sequences designed to serve as substrates for the enzyme elastase. Electrostatic repulsion of the lysine termini in KA6K prevents self-assembly, whereas in contrast KA6E is observed, through electron microscopy, to form tape-like fibrils, which based on X-ray scattering contain layers of thickness equal to the molecular length. The alanine residues enable efficient packing of the side-chains in a beta-sheet structure, as revealed by circular dichroism, FTIR and X-ray diffraction experiments. In buffer, KA6E is able to form hydrogels at sufficiently high concentration. These were used as substrates for elastase, and enzyme-induced de-gelation was observed due to the disruption of the beta-sheet fibrillar network. We propose that hydrogels of the simple designed amphiphilic peptide KA6E may serve as model substrates for elastase and this could ultimately lead to applications in biomedicine and regenerative medicine.

Distinction between semantic and episodic memory: The underlying mechanisms for autonoetic consciousness

In 1972, episodic and semantic memories were considered to reflect different types of knowledge (Tulving, 1972). However, these early definitions encountered many difficulties. Now, Episodic and semantic memories are discussed in terms of awareness associated with retrieval (Wheeler, Stuss, & Tulving, 1997): Autonoetic consciousness (i.e., feeling of remembering) is considered associated with retrieval from the episodic memory system, while noetic consciousness (i.e., feeling of knowing) is considered characterized by retrieval from the semantic memory system. The present article investigated determinants of autonoetic consciousness in order to clarify characteristics of perceptual knowledge that is being recalled, the more strongly the individual feels autonoetic consciousness during retrieval, and that autonoetic consciousness is based on rich sensory-perceptual knowledge. Furthermore, we suggested that the parietal and frontal lobes mediate the process of generating autonoetic consciousness. This suggested that sensory-perceptual knowledge, the parietal lobe and the frontal lobe are important factors for discriminating episodic memory afrom semantic memory.

Networks and plant disease management: concepts and applications

A network is a natural structure with which to describe many aspects of a plant pathosystem. The article seeks to set out in a nonmathematical way some of the network concepts that promise to be useful in managing plant disease. The field has been stimulated by developments designed to help understand and manage animal and human disease, as well as by technical infrastructures, such as the internet. It overlaps partly with landscape ecology. The study of networks has helped identify likely ways to reduce flow of disease in traded plants, to find the best sites to monitor as warning sites for annually reinvading disease, and to understand the fundamentals of how a pathogen spreads in different structures. A tension between the free flow of goods or species down communication channels and free flow of pathogens down the same pathways is highlighted.

Multiresolution semantic activity characterisation and abnormality discovery in videos

This paper addresses the issue of activity understanding from video and its semantics-rich description. A novel approach is presented where activities are characterised and analysed at different resolutions. Semantic information is delivered according to the resolution at which the activity is observed. Furthermore, the multiresolution activity characterisation is exploited to detect abnormal activity. To achieve these system capabilities, the focus is given on context modelling by employing a soft computing-based algorithm which automatically enables the determination of the main activity zones of the observed scene by taking as input the trajectories of detected mobiles. Such areas are learnt at different resolutions (or granularities). In a second stage, learned zones are employed to extract people activities by relating mobile trajectories to the learned zones. In this way, the activity of a person can be summarised as the series of zones that the person has visited. Employing the inherent soft relation properties, the reported activities can be labelled with meaningful semantics. Depending on the granularity at which activity zones and mobile trajectories are considered, the semantic meaning of the activity shifts from broad interpretation to detailed description.Activity information at different resolutions is also employed to perform abnormal activity detection.

Distributed access control and privacy for the internet of me

This article presents an experimental scalable message driven IoT and its security architecture based on Decentralized Information Flow Control. The system uses a gateway that exports SoA (REST) interfaces to the internet simplifying external applications whereas uses DIFC and asynchronous messaging within the home environment.

OntoMap: an ontology-based architecture to perform the semantic mapping between an interlingua and software components

This paper is about the use of natural language to communicate with computers. Most researches that have pursued this goal consider only requests expressed in English. A way to facilitate the use of several languages in natural language systems is by using an interlingua. An interlingua is an intermediary representation for natural language information that can be processed by machines. We propose to convert natural language requests into an interlingua [universal networking language (UNL)] and to execute these requests using software components. In order to achieve this goal, we propose OntoMap, an ontology-based architecture to perform the semantic mapping between UNL sentences and software components. OntoMap also performs component search and retrieval based on semantic information formalized in ontologies and rules.

Framtagning av lämplig teknologi för konvertering av VBA-applikation till Internet Explorer

Företaget GUTAB använder idag en frågeanalysmodul som är utvecklat i Visual Basic for Applications i Microsoft Excel. GUTAB fann det intressant att veta om modulen kunde konverteras till webbläsaren Internet Explorer samt vilken teknologi som var lämplig för detta. GUTAB var även intresserade av att veta vilken teknologi som kunde användas för att skapa grafiska manipulerbara objekt.En skrivbordsundersökning genomfördes som omfattade teknologierna ASP, ASP.NET, JSP, PHP, ActiveX samt Java applets. Skrivbordsundersökningen utgjordes av två delar där den första delen redogjorde för de två klientbaserade teknologierna ActiveX och Java applets med vilka grafiska manipulerbara objekt kunde realiseras. GUTAB ansåg att ActiveX program var mest lämpliga för detta ändamål.Baserat på skrivbordsundersökningens andra del bestämde GUTAB att ASP och ASP.NET skulle användas för att testa teknologiernas kapacitet att konvertera programlogik samt grafiska objekt från VBA-applikationen i en webbapplikation då dessa två teknologier ansågs som mest lämpliga för en konvertering.Testet som genomfördes visade att det var fullt möjligt att konvertera utvald funktionalitet med båda teknologierna. Dock kunde inte grafiska objekt skapas i asp varför befintliga objekt användes för det ändamålet. Många fördelar identifierades med ASP.NET varför GUTAB beslutade att ASP.net var den mest lämpliga teknologin att använda vid en eventuell konvertering.

Ensidesapplikationers kodkvalitet och förvaltningsbarhet : En jämförelse av förvaltningsbarhet hos single-page applications utvecklade med AngularJS och React

Single-page applications have historically been subject to strong market forces driving fast development and deployment in lieu of quality control and changeable code, which are important factors for maintainability. In this report we develop two functionally equivalent applications using AngularJS and React and compare their maintainability as defined by ISO/IEC 9126. AngularJS and React represent two distinct approaches to web development, with AngularJS being a general framework providing rich base functionality and React a small specialized library for efficient view rendering. The quality comparison was accomplished by calculating Maintainability Index for each application. Version control analysis was used to determine quality indicators during development and subsequent maintenance where new functionality was added in two steps.   The results show no major differences in maintainability in the initial applications. As more functionality is added the Maintainability Index decreases faster in the AngularJS application, indicating a steeper increase in complexity compared to the React application. Source code analysis reveals that changes in data flow requires significantly larger modifications of the AngularJS application due to its inherent architecture for data flow. We conclude that frameworks are useful when they facilitate development of known requirements but less so when applications and systems grow in size.

Semantic and Personalised Service Discovery

One of the most pervasive classes of services needed to support e-Science applications are those responsible for the discovery of resources. We have developed a solution to the problem of service discovery in a Semantic Web/Grid setting. We do this in the context of bioinformatics, which is the use of computational and mathematical techniques to store, manage, and analyse the data from molecular biology in order to answer questions about biological phenomena. Our specific application is myGrid (www.mygrid.org.uk) that is developing open source, service-based middleware upon which bioinformatics applications can be built. myGrid is specifically targeted at developing open source high-level service Grid middleware for bioinformatics.

Semantic and Personalised Service Discovery

One of the most pervasive classes of services needed to support e-Science applications are those responsible for the discovery of resources. We have developed a solution to the problem of service discovery in a Semantic Web/Grid setting. We do this in the context of bioinformatics, which is the use of computational and mathematical techniques to store, manage, and analyse the data from molecular biology in order to answer questions about biological phenomena. Our specific application is myGrid (http: //www.mygrid.org.uk) that is developing open source, service-based middleware upon which bioin- formatics applications can be built. myGrid is specif- ically targeted at developing open source high-level service Grid middleware for bioinformatics.

Evolução e segmentação no perfil dos serviços bancários pela internet

O Internet banking tem evoluído rapidamente nos últimos anos. Se em 1996 apenas alguns pioneiros se arriscavam a oferecer serviços bancários através da Web, hoje milhões de usuários no mundo todo transformaram o Internet banking numa das aplicações de maior sucesso no universo do comércio eletrônico. Entretanto, este crescimento do Internet banking não está ocorrendo de forma homogênea no mercado bancário. Dois dos fatores influenciam o grau de investimento de um banco nos serviços on-line pela Web são: porte do banco e orientação de mercado varejo ou atacado dos serviços oferecidos. Este estudo mostra que os bancos de maior porte e os serviços voltados para o varejo estão mais consolidados no uso do Internet banking, embora os bancos de menor porte e os serviços para o mercado de atacado já disponham de importantes iniciativas de uso da Internet como canal bancário.

Ontologia para moelos de negócios de redes sociais na internet: um ensaio teórico-exploratório

Atualmente, uma profusão de soluções chamadas de Web 2.0 e redes sociais está causando um grande impacto no desenvolvimento da Internet, comparando-se à era “ponto-com” em termos de crescimento, investimentos e empolgação. Estas iniciativas possuem em comum um elevado grau de formação de comunidades, e de criação e compartilhamento de conteúdo por parte do usuário, dentre outras características. Acredita-se que as redes sociais possuam um grande potencial inovador e disruptivo tanto para a sociedade quanto para o mundo empresarial. Entretanto, como transformar as redes sociais e aplicações Web 2.0 em modelos de negócios auto-sustentáveis ainda é um desafio para o mercado. Esta dissertação objetiva auxiliar a compreensão e abordagem deste tema propondo uma ontologia específica para modelos de negócios de redes sociais na internet. Isto se realiza por meio do desenvolvimento de um ensaio teórico de viés exploratório, baseado em extensa, porém não exaustiva, revisão de literatura abordando modelos de negócios, redes sociais e demais temas relacionados. A ontologia criada é então aplicada na representação de modelos de negócio de redes sociais na internet. Este trabalho também fornece uma visão geral do fenômeno Web 2.0, abordando algumas de suas principais características tecnológicas e socioeconômicas.

Os jornais brasileiros e sua atuação na internet: um estudo exploratório com base na economia da informação

A internet é considerada a mais completa manifestação da nova mídia e tem causado um impacto relevante no campo da comunicação. Os veículos de comunicação tradicionais enfrentam hoje um cenário de incerteza em relação ao futuro. As mudanças provocadas pela nova mídia criam inúmeras possibilidades, que podem ser ameaças ou novas oportunidades, mas que, em ambos os casos, exigem reações dos participantes deste mercado. Este estudo tem por objetivo identificar como os jornais diários brasileiros estão atuando no meio online em reação a estas mudanças. O processo para se atingir este objetivo ocorreu, primeiramente por meio da identificação das características e da classificação dos principais jornais brasileiros nos meios impresso e online. Em seguida, houve um aprofundamento desta pesquisa em entrevistas com profissionais deste mercado. Com base nos conceitos teóricos da economia da informação, foi construída a estrutura de uma taxonomia que permitisse uma análise mais objetiva dos veículos estudados. A avaliação dos dados coletados, tendo o apoio das informações capturadas nas entrevistas, resultou em uma análise bastante rica, da qual algumas conclusões importantes foram extraídas. Identificou-se, por exemplo, uma possível relação entre o nível de adaptação do jornal ao meio online a categoria de seu conteúdo. Outra constatação é a grande importância que o meio impresso ainda tem em termos de resultados financeiros dos jornais. Em relação ao futuro, existem muitas expectativas para o mercado de notícias, mas seu rumo ainda é muito incerto. Apesar disso, uma coisa parece clara: é importante a construção de uma marca forte baseada em credibilidade. No futuro, independentemente dos modelos de negócios que vigorarem, este será um diferencial importante para a sobrevivência dos jornais.