Functional heartburn has more in common with functional dyspepsia than with non-erosive reflux disease

INTRODUCTION: Functional dyspepsia and non-erosive reflux disease (NERD) are prevalent gastrointestinal conditions with accumulating evidence regarding an overlap between the two. Still, patients with NERD represent a very heterogeneous group and limited data on dyspeptic symptoms in various subgroups of NERD are available. AIM: To evaluate the prevalence of dyspeptic symptoms in patients with NERD subclassified by using 24 h impedance-pH monitoring (MII-pH). METHODS: Patients with typical reflux symptoms and normal endoscopy underwent impedance-pH monitoring off proton pump inhibitor treatment. Oesophageal acid exposure time (AET), type of acid and non-acid reflux episodes, and symptom association probability (SAP) were calculated. A validated dyspepsia questionnaire was used to quantify dyspeptic symptoms prior to reflux monitoring. RESULTS: Of 200 patients with NERD (105 female; median age, 48 years), 81 (41%) had an abnormal oesophageal AET (NERD pH-POS), 65 (32%) had normal oesophageal AET and positive SAP for acid and/or non-acid reflux (hypersensitive oesophagus), and 54 (27%) had normal oesophageal AET and negative SAP (functional heartburn). Patients with functional heartburn had more frequent (p<0.01) postprandial fullness, bloating, early satiety and nausea compared to patients with NERD pH-POS and hypersensitive oesophagus. CONCLUSION: The increased prevalence of dyspeptic symptoms in patients with functional heartburn reinforces the concept that functional gastrointestinal disorders extend beyond the boundaries suggested by the anatomical location of symptoms. This should be regarded as a further argument to test patients with symptoms of gastro-oesophageal reflux disease in order to separate patients with functional heartburn from patients with NERD in whom symptoms are associated with gastro-oesophageal reflux.

Convergent synthesis and cellular uptake of multivalent cell penetrating peptides derived from Tat, Antp, pVEC, TP10 and SAP

Cell penetrating peptides (CPP) are peptides of 10 to 30 residues derived from natural translocating proteins. Multivalency is known to enhance cellular uptake for the Tat peptide and closely related polycationic sequences. To test whether multivalency effects on cellular uptake might also occur with other CPP types, we prepared multivalent versions of the strongly cationic Tat, the amphipathic sequences Antp, pVEC and TP10, and the polyproline helix SAP by convergent thioether ligation of the linear CPP onto multivalent scaffolds, and evaluated their uptake in HeLa and CHO cells, intracellular localization, cytotoxicity and hemolysis. While multivalency did not increase the cellular uptake of pVEC or SAP, multivalency effects on uptake comparable to Tat were observed with TP10 and Antp, which are attributable to their polycationic nature. The efficient synthetic protocol for these divalent CPP and their localization in the cytoplasm suggest that CPP might be useful for application in cargo delivery into cells.

CHROMOSOMAL ORIGIN OF DM-DNA

Double minutes (dm) are small chromatin particles of 0.3 microns diameter found only in the metaphase cells of human and murine tumors. Dm are unique cytogenetic structures since their numbers per cell show wide variation. At cell division, dm are retained despite the lack of centromeres. In squash preparations, dm show clustering often in association with chromosomes. Human carcinoma cell line SW613-S18 was found to have large numbers of dm and biological characteristics favorable for mitotic synchronization and chromosome isolation experiments.^ S18 cells were synchronized to mitosis with metabolic and mitotic blocking compounds. Mitotic cells were lysed to release chromosomes and dm from the mitotic spindle and the resulting suspensions were fractionated to enrich for dm. The DNA in enriched fractions was characterized. The reassociation kinetics of dm-DNA driven with placental human DNA was similar to the reassociation curve of labeled placental DNA under similar conditions. In situ hybridization of dm-DNA to tumor and normal metaphase cells showed grain localization over the entire karyotype. Dm-DNA was shown by pulse chase DNA replication experiments to replicate during early and mid S-phase of the cell cycle, but not in late S-phase. In addition, BrdUrd incorporation studies showed that dm-DNA replicates only once during the S-phase. Premature chromosome condensation studies suggest the basis of numerical heterogeneity of dm is nondisjunction, not anomalous or unscheduled DNA replication.^ These data and previous cytochemical banding studies of dm in SW613-S18 indicate that dm-DNA is chromosomal in origin. No evidence of gene amplification was found in the DNA reassociation data. It is likely that dm-DNA represents the pale-staining G-band regions of the human karyotype in this cell line. ^

Sēfer Menôrat ham-mā'ôr : ... û-le-maʿan gam nāšîm we-yelādîm yiʿidû li-šmôaʿ be-limmûdîm ...

Yiṣḥāq Abôhab

ʿAmmûdê hal-lāšôn : ham-meyûssādîm ʿal adnê hā-higgāyôn le-tôʿelet hat-talmîdîm ha-ḥafeśîm li-lemôd śefat ʿēver ; di elemenṭe der hebrešn šprache nach logišn printzipien en wikelṭ ... ; ein handbuch fir lehrer

fon Joel B. J. L.

Sēfer Tiqqûnê ham-môʿādîm

[Arje Ben-Jehuda Leib Ben-Seligmann]

Šraibn an di wirdign miṭglidr zemṭlichr leblichn und wohlṭheṭign ḥavērût gemîlût-ḥasādîm be-kōl haq-qehillôt haq-qedôšôt

fon Joel Bril

Sēfer Ḥiddûšê tôrā : we-ze šemô ašer yiqre'û lô Berît Šālôm û-vô derûšîm neḥmādîm ...

ašer îzzēn ḥîbbēr we-tîqqēn ... Pinḥās .... Ben- ... Pelṭā

Abris Der Königligen Stat Stvl Weissenbvrg In Vngern : Itzvndt Von Den Christen Belegert, Anno DM̄I 1601

Kurzer Text in d. Platte graviert

Arlt y Marechal: poética y poéticas en pos del conocimiento

Este trabajo busca demostar de qué modo las respectivas poéticas de Roberto Arlt y de Leopoldo Marechal -esto es, el recorrido hacia la interioridad de lo inconsciente en el primero y el “descenso y ascenso del alma por la belleza", del segundo- en tanto conjunto de procedimientos artísticos mediante los cuales se configura la experiencia ontológica de ser en el mundo del sujeto poético, se entreteje, pese a sus diferencias individuales, con el sistema de propuestas poéticas de algunos movimientos vanguardistas y, cómo ambas totalidades coinciden con postulados generales de las teorías poéticas elaboradas por la ciencia literaria de principios de siglo, en un camino ascendente de abstracciones que muestra los procesos de interpretación de lo real que operan autor, lector y estudioso de la teoría en su particular búsqueda de la verdad a través de la comprensión y del conocimiento.

Construyendo nuevas miradas pedagógicas en pos de la accesibilidad académica en la universidad

Durante el primer cuatrimestre del año 2012 la asignatura Patrimonio Natural Regional de la carrera Tecnicatura de Empresas de Servicios Turísticos — Facultad de Turismo — Universidad Nacional del Comahue; contó con la presencia de un estudiante en situación de discapacidad (motora). A partir de la iniciativa de los docentes de la asignatura, se solicita apoyo institucional y se toma contacto con la Comisión Universitaria sobre Accesibilidad al Medio Físico y Social, para consensuar estrategias a implementar en la evaluación parcial del alumno en situación de discapacidad en relación al resto del curso. Este trabajo tiene por objetivos: reflexionar acerca de la evaluación parcial con estudiantes en situación de discapacidad y proponer cambios en la evaluación parcial de la asignatura Patrimonio Natural Regional en relación a estudiantes en situación de discapacidad motora. La metodología usada por el equipo de cátedra fue de un estudio de diseño para las asignaturas del área de concurso Recursos Naturales, contextualizado para el estudiante en situación de discapacidad con el asesoramiento de la comisión sobre accesibilidad y también de la comunicación con el alumno. Algunas conclusiones a las que se arribó tienen relación con los ejes 1. Necesidad de evaluaciones con nuevas estrategias pedagógicas contextuales particulares; 2.Necesidad de un acompañamiento institucional ante la presencia de estudiantes en situación de discapacidad en las aulas.