Dehydrin expression as a potential diagnostic tool for cold stress in white clover

Cold acclimation is important for crop survival in environments undergoing seasonal low temperatures. It involves the induction of defensive mechanisms including the accumulation of different cryoprotective molecules among which are dehydrins (DHN). Recently several sequences coding for dehydrins were identified in white clover (Trifolium repens). This work aimed to select the most responsive to cold stress DHN analogues in search for cold stress diagnostic markers. The assessment of dehydrin transcript accumulation via RT-PCR and immunodetection performed with three antibodies against the conserved K-, Y-, and S-segment allowed to outline different dehydrin types presented in the tested samples. Both analyses confirmed that YnKn dehydrins were underrepresented in the controls but exposure to low temperature specifically induced their accumulation. Strong immunosignals corresponding to 37–40 kDa with antibodies against Y- and K-segment were revealed in cold-stressed leaves. Another ‘cold-specific’ band at position 52–55 kDa was documented on membranes probed with antibodies against K-segment. Real time RT-qPCR confirmed that low temperatures induced the accumulation of SKn and YnSKn transcripts in leaves and reduced their expression in roots. Results suggest that a YnKn dehydrin transcript with GenBank ID: KC247805 and the immunosignal at 37–40 kDa, obtained with antibodies against Y- and K-segment are reliable markers for cold stress in white clover. The assessment of SKn (GenBank ID: EU846208) and YnSKn (GenBank ID: KC247804) transcript levels in leaves could serve as additional diagnostic tools.

Jacob Simonsen og Hustru Rose født Hahn og deres Forfaedre : 100 Aar efter Jacob Simonsens Fødsel

udg. af S. H. Simonsens Børn ved Josef Fischer

Die neue Rose "William Francis Bennett"

von Gebr. Schultheis

Ms. lat. qu. 65 - Le Roman de la Rose

Vorbesitzer: du Roullet

"Die Rose"

Welsch (Projektbearbeiter): Aufstellung folgender Forderungen anläßlich der Abgeordnetenwahlen zur Preußischen Nationalversammlung: gesetzliche Regelung von Einbürgerung und Meisterprüfung, Arbeitsbeschaffungsmaßnahmen, Besteuerung nach finanzieller Leistungskraft, Abschaffung von Gewerbe- und Mietssteuer. Nebst Abdruck von sieben Gedichten. Der Verfasser wurde, wie aus dem Text hervorgeht, wegen Mietschulden gerichtlich verfolgt und nicht in die Liste der Wahlberechtigten aufgenommen; letztere Maßnahme scheint jedoch - nach der Diktion der Broschüre zu urteilen - seiner beeinträchtigten geistigen Gesundheit wegen erfolgt zu sein

Der gekränkte fromme Mann und die Rose : (Nach dem Midrasch) (Gedicht)

Mose ben Zebi-Hirsch Bock

N budget and NH3 exchange of a grass/clover crop at two levels of N application

Atmospheric ammonia (NH3) exchange during a single growing season was measured over two grass/clover fields managed by cutting and treated with different rates of mineral nitrogen (N) fertilizer. The aim was to quantify the total NH3 exchange of the two systems in relation to their N budget, the latter was split into N derived from symbiotic fixation, from fertilization, and from the soil. The experimental site was located in an intensively managed agricultural area on the Swiss plateau. Two adjacent fields with mixtures of perennial ryegrass (Lolium perenne L.), cocks foot (Dactylis glomerata L.), white clover (Trifolium repens L.) and red clover (Trifolium pratense L.) were used. These were treated with either 80 or 160 kg N ha−1 applied as NH4NO3 fertilizer in equal portions after each of four cuts. Continuous NH3 flux measurements were carried out by micrometeorological techniques. To determine the contribution of each species to the overall NH3 canopy compensation point, stomatal NH3 compensation points of the individual plant species were determined on the basis of NH4+ + NH3 (NHx) concentrations and pH in the apoplast. Symbiotic N2 fixation was measured by the 15N dilution method.

Das Hotel zur weißen Rose

J. E.

Mus Hs 1913a - Prends cette rose aimable comme toi : sonnet de Ronsard

[Julius Stockhausen]

Mus Hs 1913b - Prends cette rose aimable comme toi : ode de Ronsard ; Wien, im März 1869

J. St.

Die Vorliebe der Nachtigall für die Rose

Von Fr. Wilh. Gross

Bennett's 5000-Dollar-Rose und ihr Verbreiter in Deutschland

L. Groth

Ueber die Rose

anonym

Geschichte, Geographie und Poesie der Rose

anonym

Die Rose "Kaiserin Auguste Viktoria"

Von J. Barfuss