Resistance to human immunodeficiency virus type 1 infection conferred by transduction of human peripheral blood lymphocytes with ribozyme, antisense, or polymeric trans-activation response element constructs.

Human peripheral blood lymphocytes (PBLs) were transduced with a number of recombinant retroviruses including RRz2, an LNL6-based virus with a ribozyme targeted to the human immunodeficiency virus (HIV) tat gene transcript inserted within the 3' region of the neomycin-resistance gene; RASH5, and LNHL-based virus containing an antisense sequence to the 5' leader region of HIV-1 downstream of the human cytomegalovirus promoter; and R20TAR, an LXSN-based virus with 20 tandem copies of the HIV-1 trans-activation response element sequence driven by the Moloney murine leukemia virus long terminal repeat. After G418 selection, transduced PBLs were challenged with the HIV-1 laboratory strain IIIB and a primary clinical isolate of HIV-1, 82H. Results showed that PBLs from different donors could be transduced and that this conferred resistance to HIV-1 infection. For each of the constructs, a reduction of approximately 70% in p24 antigen level relative to the corresponding control-vector-transduced PBLs was observed. Molecular analyses showed constitutive expression of all the transduced genes from the retroviral long terminal repeat, but no detectable transcript was seen from the internal human cytomegalovirus transcript was seen from the internal human cytomegalovirus promoter for the antisense construct. Transduction of, and consequent transgene expression in, PBLs did not impact on the surface expression of either CD4+/CD8+ (measured by flow cytometry) or on cell doubling time (examined by [3H]thymidine uptake). These results indicate the potential utility of these anti-HIV-1 gene therapeutic agents and show the preclinical value of this PBL assay system.

Implementation of non-local boundary layer schemes in the Regional Atmospheric Modeling System and its impact on simulated mesoscale circulations

This paper proposes the implementation of different non-local Planetary Boundary Layer schemes within the Regional Atmospheric Modeling System (RAMS) model. The two selected PBL parameterizations are the Medium-Range Forecast (MRF) PBL and its updated version, known as the Yonsei University (YSU) PBL. YSU is a first-order scheme that uses non-local eddy diffusivity coefficients to compute turbulent fluxes. It is based on the MRF, and improves it with an explicit treatment of the entrainment. With the aim of evaluating the RAMS results for these PBL parameterizations, a series of numerical simulations have been performed and contrasted with the results obtained using the Mellor and Yamada (MY) scheme, also widely used, and the standard PBL scheme in the RAMS model. The numerical study carried out here is focused on mesoscale circulation events during the summer, as these meteorological situations dominate this season of the year in the Western Mediterranean coast. In addition, the sensitivity of these PBL parameterizations to the initial soil moisture content is also evaluated. The results show a warmer and moister PBL for the YSU scheme compared to both MRF and MY. The model presents as well a tendency to overestimate the observed temperature and to underestimate the observed humidity, considering all PBL schemes and a low initial soil moisture content. In addition, the bias between the model and the observations is significantly reduced moistening the initial soil moisture of the corresponding run. Thus, varying this parameter has a positive effect and improves the simulated results in relation to the observations. However, there is still a significant overestimation of the wind speed over flatter terrain, independently of the PBL scheme and the initial soil moisture used, even though a different degree of accuracy is reproduced by RAMS taking into account the different sensitivity tests.

Cast iron pipe /

Copy in Dept. of Rare Books and Special Collections bound in original black leather stamped in gold. P. 9-10 lacking.

Pam decides : a sequel to "Pam" /

Colored frontispiece and plates facing p. 26, 114, 142, 248 and 302.

Das Auswanderungsbuch, oder, Führer und Rathgeber bei der Auswanderung nach Nordamerika und Texas : in Bezug auf Ueberfahrt, Ankunft und Ansiedelung nebst einer vollständigen Schilderung des geographischen, politischen und geselligen Zustandes jener Länder und genauer Erörterung aller bei der Auswanderung zu berücksichtigenden Punkte : grösstentheils nach eigener Auffassung während eines zwejährigen Aufenthalts in Amerika /

Ownership mark, front flyleaf: Paul Vogt.

The American debater : being a plain exposition of the principles and practice of public debate, wherein will be found an account of the qualifications necessary to a good deliberative orator, as also the mode of acquiring them, the rules of order observed in deliberative assemblies, debates in full and in outline, on various interesting topics, numerous questions for discussion, forms of a constitution for literary clubs or debating societies, etc., etc. /

Publishers include Moore, Wilstach, Keys & Co., Cincinnati.

Pinneo's exercises in false syntax : for the correction of errors in the grammatical construction of sentences : designed to aid in the study of the author's grammars of the English language /

Copy in Dept. of Rare Books and Special Collections has original pink printed boards with black leather spine.

The girl from Girton : and other stories about schools /

Cover title: Stories of the school room.

In the name of liberty : a story of the terror /

Sepia frontispiece, signed by A. Castaigne.

Thirty pieces of silver /

Frontispiece and t.p. on double leaf plate, plates facing p. 4 and 20.

Holiday stories for young people /

Mode of access: Internet.

Sallust.

On cover: New York, J & J Harper, 1836.

The riddle of the night : being the record of a singular adventure of that remarkable detective genius, Hamilton Cleek, the man of the forty faces, once known to the police as the Vanishing Cracksman /

Frontispiece and plates facing p. 84, 146 and 300, illustrations on p. 20, 157 and 159.

Laramie holds the range /

Advertisement on p. [2] of preliminary p.

A century of gossip, or, The real and the seeming /

At head of title: New England life.