Structural determinants of subunit -subunit interactions and subcellular localization of the calcium /calmodulin -dependent protein kinase II

The multifunctional Ca$\sp{2+}$/calmodulin-dependent protein kinase II (CaM kinase) is a Ser/Thr directed protein kinase that participates in diverse Ca$\sp{2+}$ signaling pathways in neurons. The function of CaM kinase depends upon the ability of subunits to form oligomers and to interact with other proteins. Oligomerization is required for autophosphorylation which produces significant functional changes that include Ca$\sp{2+}$/calmodulin-independent activity and calmodulin trapping. Associations with other proteins localize CaM kinase to specific substrates and effectors which serves to optimize the efficiency and speed of signal transduction. In this thesis, we investigate the interactions that underlie the appropriate positioning of CaM kinase activity in cells. We demonstrate that the subcellular distribution of CaM kinase is dynamic in hippocampal slices exposed to anoxic/aglycemic insults and to high K$\sp{+}$-induced depolarization. We determine the localization of CaM kinase domains expressed in neurons and PC-12 cells and find that the C-terminal domain of the $\alpha$ subunit is necessary for localization to dendrites. Moreover, monomeric forms of the enzyme gain access to the nucleus. Attempts made to identify novel CaM kinase binding proteins using the yeast two-hybrid system resulted in the isolation of hundreds of positive clones. Those that have been sequenced are identical to CaM kinase isoforms. Finally, we report the discovery of specific regions within the C-terminal domain that are necessary and sufficient for subunit-subunit interactions. Differences between the $\alpha$ and $\beta$ isoforms were discovered that indicate unique structural requirements for oligomerization. A model for how CaM kinase subunits interact to form holoenzymes and how structural heterogeneity might influence CaM kinase function is presented. ^

Description and chemical composition of ferromanganese encrustations and deposits in the Atlantic Ocean from R/V Atlantis II cruises 20, 42, 60, 85; R/V Chain cruise 35; R/V Knorr cruise 61 and RRS Shackleton cruise 75/1 stations

Chemical and mineralogical compositions of ferromanganese oxide coatings on rocks dredged from the New England Seamounts, the Sierra Leone Rise and the Mid-Atlantic Ridge near the Equator have been determined in an investigation of regional differences in Atlantic ferromanganese deposits. Most encrustations are clearly of hydrogenous origin, consisting mainly of todorokite and delta MnO2, but several recovered from the equatorial fracture zones may be hydrothermal accumulations. Differences in the chemistry of the water column and in growth rates of the ferromanganese coatings may be important in producing this regional contrast in composition. Fine-scale changes in element abundances within the encrustations indicate that the nature of the substrate has little influence on compositional variations.

Water temperature reconstruction for Terminations I and II of sediment core MD01-2378

We present centennial records of sea surface and upper thermocline temperatures in Core MD01-2378 from the Timor Sea, which provide new insights into the variability of the Indonesian outflow across the last two glacial terminations. Mg/Ca in Globigerinoides ruber (white s. s.) indicates an overall increase of 3.2 °C in sea surface temperature (SST) over Termination I. Following an early Holocene plateau at 11.3-6.4 ka, SSTs cooled by 0.6 °C during the middle to late Holocene (6.4-0.7 ka). The early Holocene warming occurred in phase with increasing northern hemisphere summer insolation, coinciding with northward displacement of the Intertropical Convergence Zone, enhanced boreal summer monsoon and expansion of the Indo-Pacific Warm Pool. Thermocline temperatures (Pulleniatina obliquiloculata Mg/Ca) gradually decreased from 24.5 to 21.5 °C since 10.3 ka, reflecting intensification of a cool thermocline throughflow. The vertical structure of the upper ocean in the Timor Sea evolved in similar fashion during the Holocene and MIS5e, although the duration of SST plateaux differed (11.3 to 6.4 ka in Termination I and from 129 to 119 ka in Termination II), which was probably due to the more intense northern hemisphere summer insolation during MIS 5e. During both terminations, SST increased simultaneously in the southern high latitudes and the tropical eastern Indian Ocean, suggesting virtually instantaneous atmospheric climate feedbacks between the high and low latitudes.

Plio-Pleistocene Mg/Ca and TEX86 sea surface temperature records of ODP Sites 184-1143 and 165-999,

The western warm pools of the Atlantic and Pacific oceans are a critical source of heat and moisture for the tropical climate system. Over the past five million years, global mean temperatures have cooled by 3-4 °C. Yet, current reconstructions of sea surface temperatures indicate that temperature in the warm pools has remained stable during this time. This stability has been used to suggest that tropical sea-surface temperatures are controlled by some sort of thermostat-like regulation. Here we reconstruct sea surface temperatures in the South China Sea, Caribbean Sea and western equatorial Pacific Ocean for the past five million years, using a combination of the Mg/Ca, TEXH86-and Uk'37 surface temperature proxies. Our data indicate that during the period of Pliocene warmth from about 5 to 2.6 million years ago, the western Pacific and western Atlantic warm pools were about 2 °C warmer than today. We suggest that the apparent lack of warming seen in the previous reconstructions was an artefact of low seawater Mg/Ca ratios in the Pliocene oceans. Taking this bias into account, our data indicate that tropical sea surface temperatures did change in conjunction with global mean temperatures. We therefore conclude that the temperature of the warm pools of the equatorial oceans during the Pliocene was not limited by a thermostat-like mechanism.

X-ray fluorescence (XRF) Ca intensity of sediment cores MD04-2760 and MD04-2788

Accelerator mass spectrometry (AMS) radiocarbon dating of ostracod and gastropod shells from the southwestern Black Sea cores combined with tephrochronology provides the basis for studying reservoir age changes in the lateglacial Black Sea. The comparison of our data with records from the northwestern Black Sea shows that an apparent reservoir age of ~1450 14C yr found in the glacial is characteristic of a homogenized water column. This apparent reservoir age is most likely due to the hardwater effect. Though data indicate that a reservoir age of ~1450 14C yr may have persisted until the Bølling-Allerød warm period, a comparison with the GISP2 ice-core record suggests a gradual reduction of the reservoir age to ~1000 14C yr, which might have been caused by dilution effects of inflowing meltwater. During the Bølling-Allerød warm period, soil development and increased vegetation cover in the catchment area of the Black Sea could have hampered erosion of carbonate bedrock, and hence diminished contamination by "old" carbon brought to the Black Sea basin by rivers. A further reduction of the reservoir age most probably occurred contemporary to the precipitation of inorganic carbonates triggered by increased phytoplankton activity, and was confined to the upper water column. Intensified deep water formation subsequently enhanced the mixing/convection and renewal of intermediate water. During the Younger Dryas, the age of the upper water column was close to 0 yr, while the intermediate water was ~900 14C yr older. The first inflow of saline Mediterranean water, at ~8300 14C yr BP, shifted the surface water age towards the recent value of ~400 14C yr.

O corpo esportivo e o nacionalismo brasileiro durante a II Guerra Mundial : Uma análise a partir de um jornal da cidade de Vitória/ES/Brasil

Este artigo discute a relaçao entre esporte e nacionalismo, investigando a importância atribuída às práticas esportivas decorrentes desse vínculo. A análise se materializou entre os anos de 1939 e 1945, por meio do jornal "A Tribuna", publicaçao diária da cidade de Vitória, capital do Espírito Santo. Além de o esporte se configurar como uma escola de cultura física, constituiu-se, também, em uma escola de formaçao cívica da juventude, materializando os desejos do governo para a construçao da Naçao brasileira

Amistades paganas y persecución de los cristianos : La epístola de Bruno de Querfurt al emperador Enrique II

La carta que presentamos traducida al español fue escrita en el año 1008 por el obispo misionero Bruno de Querfurt. El destinatario era el rey sajón Enrique II (1002-1024), emperador desde el año 1014. El contexto de la epístola está marcado por los vínculos cambiantes entre diversos actores políticos. La dinastía polaca piasta había cooperado con el Imperio desde mediados del siglo x. Por otra parte, los liutizos, una confederación de pueblos eslavos paganos, se habían levantado contra las estructuras eclesiásticas e imperiales en la región del Elba en 983, en el marco de un floreciente paganismo. Sin embargo, al iniciarse el nuevo milenio, el piasta Boleslao el Bravo se había convertido en una amenaza más atemorizadora para el Imperio, debido particularmente a la expansión territorial que encabezaba. Así, el rey Enrique II pactó en el año 1003 en Quedlinburg con los liutizos frente al piasta: se sellaba una alianza entre un rey cristiano y una confederación pagana para hacer frente a otro gobernante cristiano. Bruno de Querfurt, que había dedicado su vida a la misión y recibía apoyo de Boleslao, escribió esta misiva, con el objeto de señalar las contradicciones de la alianza citada arriba

Amistades paganas y persecución de los cristianos : La epístola de Bruno de Querfurt al emperador Enrique II

La carta que presentamos traducida al español fue escrita en el año 1008 por el obispo misionero Bruno de Querfurt. El destinatario era el rey sajón Enrique II (1002-1024), emperador desde el año 1014. El contexto de la epístola está marcado por los vínculos cambiantes entre diversos actores políticos. La dinastía polaca piasta había cooperado con el Imperio desde mediados del siglo x. Por otra parte, los liutizos, una confederación de pueblos eslavos paganos, se habían levantado contra las estructuras eclesiásticas e imperiales en la región del Elba en 983, en el marco de un floreciente paganismo. Sin embargo, al iniciarse el nuevo milenio, el piasta Boleslao el Bravo se había convertido en una amenaza más atemorizadora para el Imperio, debido particularmente a la expansión territorial que encabezaba. Así, el rey Enrique II pactó en el año 1003 en Quedlinburg con los liutizos frente al piasta: se sellaba una alianza entre un rey cristiano y una confederación pagana para hacer frente a otro gobernante cristiano. Bruno de Querfurt, que había dedicado su vida a la misión y recibía apoyo de Boleslao, escribió esta misiva, con el objeto de señalar las contradicciones de la alianza citada arriba

O corpo esportivo e o nacionalismo brasileiro durante a II Guerra Mundial : Uma análise a partir de um jornal da cidade de Vitória/ES/Brasil

Este artigo discute a relaçao entre esporte e nacionalismo, investigando a importância atribuída às práticas esportivas decorrentes desse vínculo. A análise se materializou entre os anos de 1939 e 1945, por meio do jornal "A Tribuna", publicaçao diária da cidade de Vitória, capital do Espírito Santo. Além de o esporte se configurar como uma escola de cultura física, constituiu-se, também, em uma escola de formaçao cívica da juventude, materializando os desejos do governo para a construçao da Naçao brasileira

Amistades paganas y persecución de los cristianos : La epístola de Bruno de Querfurt al emperador Enrique II

La carta que presentamos traducida al español fue escrita en el año 1008 por el obispo misionero Bruno de Querfurt. El destinatario era el rey sajón Enrique II (1002-1024), emperador desde el año 1014. El contexto de la epístola está marcado por los vínculos cambiantes entre diversos actores políticos. La dinastía polaca piasta había cooperado con el Imperio desde mediados del siglo x. Por otra parte, los liutizos, una confederación de pueblos eslavos paganos, se habían levantado contra las estructuras eclesiásticas e imperiales en la región del Elba en 983, en el marco de un floreciente paganismo. Sin embargo, al iniciarse el nuevo milenio, el piasta Boleslao el Bravo se había convertido en una amenaza más atemorizadora para el Imperio, debido particularmente a la expansión territorial que encabezaba. Así, el rey Enrique II pactó en el año 1003 en Quedlinburg con los liutizos frente al piasta: se sellaba una alianza entre un rey cristiano y una confederación pagana para hacer frente a otro gobernante cristiano. Bruno de Querfurt, que había dedicado su vida a la misión y recibía apoyo de Boleslao, escribió esta misiva, con el objeto de señalar las contradicciones de la alianza citada arriba

O corpo esportivo e o nacionalismo brasileiro durante a II Guerra Mundial : Uma análise a partir de um jornal da cidade de Vitória/ES/Brasil

Este artigo discute a relaçao entre esporte e nacionalismo, investigando a importância atribuída às práticas esportivas decorrentes desse vínculo. A análise se materializou entre os anos de 1939 e 1945, por meio do jornal "A Tribuna", publicaçao diária da cidade de Vitória, capital do Espírito Santo. Além de o esporte se configurar como uma escola de cultura física, constituiu-se, também, em uma escola de formaçao cívica da juventude, materializando os desejos do governo para a construçao da Naçao brasileira

X-ray fluorescence scanning of discrete samples on the Schwalbenberg II loess-paleosol sequence, raw data, magnetic susceptibility, organic carbon and U-ratio as grainsize index

The Schwalbenberg II loess-paleosol sequence (LPS) denotes a key site for Marine Isotope Stage (MIS 3) in Western Europe owing to eight succeeding cambisols, which primarily constitute the Ahrgau Subformation. Therefore, this LPS qualifies as a test candidate for the potential of temporal high-resolution geochemical data obtained X-ray fluorescence (XRF) scanning of discrete samplesproviding a fast and non-destructive tool for determining the element composition. The geochemical data is first contextualized to existing proxy data such as magnetic susceptibility (MS) and organic carbon (Corg) and then aggregated to element log ratios characteristic for weathering intensity [LOG (Ca/Sr), LOG (Rb/Sr), LOG (Ba/Sr), LOG (Rb/K)] and dust provenance [LOG (Ti/Zr), LOG (Ti/Al), LOG (Si/Al)]. Generally, an interpretation of rock magnetic particles is challenged in western Europe, where not only magnetic enhancement but also depletion plays a role. Our data indicates leaching and top-soil erosion induced MS depletion at the Schwalbenberg II LPS. Besides weathering, LOG (Ca/Sr) is susceptible for secondary calcification. Thus, also LOG (Rb/Sr) and LOG (Ba/Sr) are shown to be influenced by calcification dynamics. Consequently, LOG (Rb/K) seems to be the most suitable weathering index identifying the Sinzig Soils S1 and S2 as the most pronounced paleosols for this site. Sinzig Soil S3 is enclosed by gelic gleysols and in contrast to S1 and S2 only initially weathered pointing to colder climate conditions. Also the Remagen Soils are characterized by subtle to moderate positive excursions in the weathering indices. Comparing the Schwalbenberg II LPS with the nearby Eifel Lake Sediment Archive (ELSA) and other more distant German, Austrian and Czech LPS while discussing time and climate as limiting factors for pedogenesis, we suggest that the lithologically determined paleosols are in-situ soil formations. The provenance indices document a Zr-enrichment at the transition from the Ahrgau to the Hesbaye Subformation. This is explained by a conceptual model incorporating multiple sediment recycling and sorting effects in eolian and fluvial domains.