Adjustment of homeless adolescents to a crisis shelter: Application of a stress and coping model

The present study examined the utility of a stress and coping model of adaptation to a homeless shelter among homeless adolescents. Seventy-eight homeless adolescents were interviewed and completed self-administered scales at Time 1 (day of shelter entry) and Time 2 (day of discharge). The mean duration of stay at the shelter was 7.23 days (SD = 7.01). Predictors included appraisal (threat and self-efficacy), coping resources, and coping strategies (productive, nonproductive, and reference to others coping). Adjustment outcomes were Time I measures of global distress, physical health, clinician-and youthworker- rated social adjustment, and externalizing behavior and Time 2 youthworker-rated social adjustment and goal achievement. Results of hierarchical regression analyses indicated that after controlling for the effects of relevant background variables (number of other shelters visited, sexual, emotional, and physical abuse), measures of coping resources, appraisal, and coping strategies evidenced distinct relations with measures of adjustment in ways consistent with the model's predictions with few exceptions. In cross-sectional analyses better Time I adjustment was related to reports of higher levels of coping resources, self-efficacy beliefs, and productive coping strategies, and reports of lower levels of threat appraisal and nonproductive coping strategies. Prospective analyses showed a link between reports of higher levels of reference to others coping strategies and greater goal achievement and, unexpectedly, an association between lower self-efficacy beliefs and better Time 2 youthworker-rated social adjustment. Hence, whereas prospective analyses provide only limited support for the use of a stress and coping model in explaining the adjustment of homeless adolescents to a crisis shelter, cross-sectional findings provide stronger support.

Adjustment to chronic obstructive pulmonary disease: the importance of psychological factors

Research has indicated a weak relationship between the degree of physical problems and quality of life in patients with chronic obstructive pulmonary disease (COPD). The importance of adaptive psychological functioning to maintain optimum quality of life has long been recognized, but there is a lack of empirical evidence concerning the nature of psychological factors involved in adjustment to COPD. Ninety-two males completed questionnaires to determine their coping strategies, levels of self-efficacy of symptom management and social support. Adjustment was measured in terms of depression, anxiety and quality of life. Symptom severity, socioeconomic status, duration of disease and age, which have been demonstrated to be of consequence in COPD, were used as control variables in hierarchical multiple regression analyses. Higher levels of catastrophic withdrawal coping strategies and lower levels of self-efficacy of symptom management were associated with higher levels of depression, anxiety and a reduced quality of life. Higher levels of positive social support were linked to lower levels of depression and anxiety, while higher levels of negative social support were linked to higher levels of depression and anxiety. To maximize quality of life in patients with chronic obstructive pulmonary disease, psychological factors need to be carefully assessed and addressed.

Rab23, a negative regulator of hedgehog signaling, localizes to the plasma membrane and the endocytic pathway

The regulation of hedgehog signaling by vesicular trafficking was exemplified by the finding that Rab23, a Rab-GTPase vesicular transport protein, is mutated in open brain mice. In this study, the localization of Rab23 was analyzed by light and immunoelectron microscopy after expression of wild-type (Rab23-GFP), constitutively active Rab23 (Rab23Q68L-GFP), and inactive Rab23 (Rab23S23N-GFP) in a range of mammalian cell types. Rab23-GFP and Rab23Q68L-GFP were predominantly localized to the plasma membrane but were also associated with intracellular vesicular structures, whereas Rab23S23N-GFP was predominantly cytosolic. Vesicular Rab23-GFP colocalized with Rab5Q79L and internalized transferrin-biotin, but not with a marker of the late endosome or the Golgi complex. To investigate Rab23 with respect to members of the hedgehog signaling pathway, Rab23-GFP was coexpressed with either patched or smoothened. Patched colocalized with intracellular Rab23-GFP but smoothened did not. Analysis of patched distribution by light and immunoelectron microscopy revealed it is primarily localized to endosomal elements, including transferrin receptor-positive early endosomes and putative endosome carrier vesicles and, to a lesser extent, with LBPA-positive late endosomes, but was excluded from the plasma membrane. Neither patched or smoothened distribution was altered in the presence of wild-type nor mutant Rab23-GFP, suggesting that despite the endosomal colocalization of Rab23 and patched, it is likely that Rab23 acts more distally in regulating hedgehog signaling.

Is emotional intelligence training for leaders justified?

In this paper, we present the results of a qualitative study of subordinate perceptions of leaders. The study represents a preliminary test of a model based on Affective Events Theory, which posits that leaders who are seen to be effective shape the affective events that determine employees' attitudes and behaviours in the workplace. Within this framework, we argue that effective leaders ameliorate employees' hassles by providing frequent, small emotional uplifts. The resulting positive affective states are then proposed to lead to more positive employee attitudes and behaviours, and more positive regard for the leader. Importantly, leaders who demonstrate these ameliorating behaviours are likely to require high levels of emotional intelligence, defined in terms of the ability to recognise, understand, and manage emotions in self and others. To investigate this model, we conducted interviews and focus groups with 10 leaders and 24 employees. Results confirmed that these processes do indeed exist in the workplace. In particular, leaders who were seen by employees to provide continuous small emotional uplifts were consistently held to be the most effective. Study participants were especially affected by negative events (or hassles). Leaders who failed to deal with hassles or, worse still, were the source of hassles, were consistently seen to be less effective. We conclude with a discussion of implications for practicing managers, and suggest that our exploratory findings provide justification for emotional intelligence training as a means to improve leader perceptions and effectiveness. [Abstract from author]

The use of 16s rDNA restriction fragment length polymorphism analysis for the identification of non-fermenting gram negative bacilli in a cystic fibrosis microbiology referral service

The utility of 16s rDNA restriction fragment length polymorphism (RFLP) analysis for the partial genomovar differentiation of Burkholderia cepacia complex bacterium is well documented. We compared the 16s rDNA RFLP signatures for a number of non-fermenting gram negative bacilli (NF GNB) LMG control strains and clinical isolates pertaining to the genera Burkholderia, Pseudomonas, Achromobacter (Alcaligenes), Ralstonia, Stenotrophomonas and Pandoraea. A collection of 24 control strain (LMG) and 25 clinical isolates were included in the study. Using conventional PCR, a 1.2 kbp 16s rDNA fragment was generated for each organism. Following restriction digestion and electrophoresis, each clinical isolate RFLP signature was compared to those of the control strain panel. Nineteen different RFLP signatures were detected from the 28 control strains included in the study. TwentyoneyTwenty- five of the clinical isolates could be classified by RFLP analysis into a single genus and species when compared to the patterns produced by the control strain panel. Four clinical B. pseudomallei isolates produced RFLP signatures which were indistinguishable from B. cepacia genomovars I, III and VIII. The identity of these four isolates were confirmed using B. pseudomallei specific PCR. 16s rDNA RFLP analysis can be a useful identification strategy when applied to NF GNB, particularly for those which exhibit colistin sulfate resistance. The use of this molecular based methodology has proved very useful in the setting of a CF referral laboratory particularly when utilised in conjunction with B. cepacia complex and genomovar specific PCR techniques. Species specific PCR or sequence analysis should be considered for selected isolates; especially where discrepancies between epidemiology, phenotypic and genotypic characteristics occur.

The genetics of glycosylation in Gram-negative bacteria

In recent years there has been a dramatic increase in reports of glycosylation of proteins in various Gram-negative systems including Neisseria meningitidis, Neisseria gonorrhoeae, Campylobacter jejuni, Pseudomonas aeruginosa, Escherichia coli, Caulobacter crescentus, Aeromonas caviae and Helicobacter pylori. Although this growing list contains many important pathogens (reviewed by Benz and Schmidt [Mol. Microbiol. 45 (2002) 267-276]) and the glycosylations are found on proteins important in pathogenesis such as pili, adhesins and flagella the precise role(s) of the glycosylation of these proteins remains to be determined. Furthermore, the details of the glycosylation biosynthetic process have not been determined in any of these systems. The definition of the precise role of glycosylation and the mechanism of biosynthesis will be facilitated by a detailed understanding of the genes involved. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Organisational and professional commitments: the influence in nurses´organizational citizenship behaviours

The way an employee behaves in his work can be influenced by the organisational and professional commitment. Nurses are professionals who are guided by organisational and professional goals and values. Among nurses, professional commitment may be an important antecedent of organisational citizenship behaviours. The study shows how organisational and professional commitment is related with nurses’ organisational citizenship behaviours. Data from a sample of 420 nurses working in two hospitals --- the Hospital of St. Marcos, Braga and the Hospital Centre of Alto Ave, Guimarães and Fafe units were collected. The main findings are as follows: (a) organisational commitment and professional commitment contribute to the explanation of nurses’ organisational citizenship behaviours, (b) affective organisational commitment, continuance organisational commitment --- personal sacrifice, affective professional commitment and continuance professional commitment explain 28.6% of variance of organisational citizenship behaviours. © 2013 Instituto Politécnico do Cávado e do Ave (IPCA). Published by Elsevier España, S.L.U. All rights reserved.

Estudo das diferenças de género nas cognições antecipatórias, estratégias de coping e depressão em adultos

RESUMO: A presente dissertação tem o objectivo de estudar a relação das diferenças de género nas cognições antecipatórias, estratégias de coping e depressão. Para o efeito, constituiu-se duas amostras, uma de indivíduos do género feminino (n=224) de 57,9% (com uma média de idades de 36,2 e um DP=10,5), e outra por indivíduos do género masculino (n=163), perfazendo uma percentagem de 42,1% (com uma média de idades de 41,1 e um DP=12,5). Foi elaborado um protocolo de investigação composto por: Questionário de dados sócio-demográficos, Questionário de Cognições Antecipatórias (QCA), de Figueira & Ramos, 1995, o Questionário de Modos de Lidar com os Acontecimentos (QMLA), de J. Pais Ribeiro, C. Santos, 2001 e o Inventário de Avaliação Clínica da Depressão (IACLIDE), de A, Vaz Serra, 1995. Os resultados demonstraram que não existem diferenças estatisticamente significativas entre géneros nas cognições antecipatórias (p=0,594). Em relação às estratégias de coping, os resultados foram estatisticamente significativos, com as mulheres a recorrerem mais à procura de suporte social (p=0,042) e à fuga-evitamento (p=0,006). O índice de depressão mostra, de forma estatisticamente significativa, que o género feminino apresenta valores mais elevados (p=0,038). A escala de depressão permitiu ainda verificar de forma estatisticamente significativa que as mulheres revelam mais dificuldades no desempenho das tarefas associadas a queixas biológicas e cognitivas (p=0,003), tal como nas dimensões biológica (p=0,002) e de desempenho da tarefa (p=0,007). A relação entre as três variáveis, permite concluir que o índice global da escala de depressão está relacionado positivamente com as Cognições Antecipatórias (p=,000), e nas estratégias de coping, as dimensões fuga-evitamento (p=,002), resolução planeada do problema (p=,000) e a reavaliação positiva (p=,034) também estão relacionadas com esta escala da depressão de forma estatisticamente significativa. ABSTRACT: This dissertation aims to study the relationship of gender differences in anticipatory cognitions, coping strategies and depression. For this purpose, it was constituted two samples, one for female gender (n = 224) 57.9% (with a mean age of 36.2 and a SD=10.5), and other of male gender (n = 163), giving a percentage of 42.1% (with an average age of 41.1 and a SD=12.5). It was prepared a research protocol composed of data questionnaire included social-demographic, Anticipatory Cognitions Questionnaire (QCA), Figueira & Ramos, 1995, the Ways of Coping Questionnaire (WCQ), J. Pais Ribeiro, C. Santos, 2001, and the Inventory and Evaluation of Clinical Depression (IACLIDE) A, Vaz Serra, 1995. The results have shown no statistically significant differences between genders in anticipatory cognitions (p= 0.594). In the coping strategies, the results were statistically significant, with women looking to take more advantage of social support (p= 0.042) and escape-avoidance (p= 0.006). The rate of depression shows a statistically significant with females having higher values (p=0.038). The depression scale allowed us to verify statistically significant that women shows more difficulty in performing tasks associated with biological and cognitive complaints (p=0,003), as in the biological (p=0,002) and task performance (p=0,007). The relationship between the three variables, shows that the overall rate of depression scale is positively related to the Anticipatory Cognitions (p =, 000), and strategies for coping, escape-avoidance dimensions (p =, 002), resolution of the planned problem (p =, 000) and positive reappraisal (p =, 034) are also related to the scale of depression and were also statistically significant.

A influência do modelo pedagógico em variáveis psicológicas de estudantes do 1º ano da ESTSP-IPP

Introdução – A adaptação ao ensino superior reveste-se de experiências académicas que podem constituir fonte de stress para os estudantes. A implementação de novos modelos pedagógicos, no âmbito do processo de Bolonha, introduz novas variáveis cujo impacto, designadamente em termos de saúde, importa conhecer. Este estudo tem como objetivo analisar as associações entre modelo pedagógico (Problem Based Learning – PBL vs. modelos próximos do tradicional) e variáveis psicológicas (coping, desregulação emocional, sintomas psicossomáticos, perceção de stress e afeto). Metodologia – O estudo tem um design transversal. Foram usados os seguintes questionários online: Brief-COPE, Escala de Dificuldades de Regulação Emocional, Questionário de Manifestações Físicas de Mal-Estar, Escala de Stress Percebido e Escala de Afeto Positivo e Negativo. A amostra é constituída por 183 estudantes do primeiro ano (84% do género feminino) de cursos da Escola Superior de Tecnologia da Saúde do Porto – Instituto Politécnico do Porto (ESTSP-IPP). Resultados – Foram encontradas correlações significativas entre as variáveis demográficas e psicológicas. Considerando diferentes modelos pedagógicos, foram encontradas diferenças significativas nas variáveis psicológicas. Os principais preditores de stress na amostra foram: ser mulher, frequentar uma licenciatura no modelo PBL, ter maiores índices de desregulação emocional, apresentar mais sintomas psicossomáticos, menos afeto positivo e mais afeto negativo. Conclusão – As diferenças encontradas entre modelos pedagógicos são discutidas, possibilitando a reflexão sobre as implicações práticas e sugestões para futuras investigações.

Negative modulation of human osteoclastogenesis by antiepileptic drugs

Bone is constantly being molded and shaped by the action of osteoclasts and osteoblasts. A proper equilibrium between both cell types metabolic activities is required to ensure an adequate skeletal tissue structure, and it involves resorption of old bone and formation of new bone tissue. It is reported that treatment with antiepileptic drugs (AEDs) can elicit alterations in skeletal structure, in particular in bone mineral density. Nevertheless, the knowledge regarding the effects of AEDs on bone cells are still scarce, particularly on osteoclastic behaviour. In this context, the aim of this study was to investigate the effects of five different AEDs on human osteoclastic cells. Osteoclastic cell cultures were established from precursor cells isolated from human peripheral blood, and were maintained in the absence (control) or in the presence of 10-8-10-4 M of different AEDs (valproate, carbamazepine, gabapentin, lamotrigine and topiramate). Cell cultures were characterized throughout a 21-day period for tartrate-resistant acid phosphatase (TRAP) activity, number of TRAP+ multinucleated cells, presence of cells with actin rings and expressing vitronectin and calcitonin receptors, and apoptosis rate. Also, the involvement of several signaling pathways on the cellular response was addressed. All the tested drugs were able to affect osteoclastic cell development, although with different profiles on their osteoclastogenic modulation properties. Globally, the tendency was to inhibit the process. Furthermore, the signaling pathways involved in the process also seemed to be differentially affected by the AEDs, suggesting that the different drugs may affect osteoclastogenesis through different mechanisms. In conclusion, the present study showed that the different AEDs had the ability to negatively modulate the osteoclastogenesis process, shedding new light towards a better understanding of how these drugs can affect bone tissue.

Stresse, burnout e estratégias de coping nos guardas prisionais da Região Autónoma dos Açores

Mestrado, Psicologia da Educação (Contextos Educativos), 18 de Junho de 2014, Universidade dos Açores.

Riscos psicossociais no trabalho: estresse e estratégias de coping em enfermeiros em oncologia

Objetivo: identificar fontes de estresse e estratégias de coping em enfermeiros que exercem funções em três Serviços de Oncologia de Cirurgia Cabeça e Pescoço, de três hospitais centrais de Portugal. Método: estudo transversal, de carácter descritivo e exploratório, cuja amostra foi constituída pelos 96 enfermeiros dos três serviços. Na recolha de dados, foram utilizados: Questionário Sociodemográfico, Questionário de Saúde Geral-12, Inventário de Estressores Ocupacionais e Brief COPE. Resultados: verificaram-se níveis razoáveis de saúde geral. Os estressores mais referidos foram: sobrecarga de trabalho, baixa remuneração salarial, espaço físico onde se desenvolve a profissão, situações emocionalmente perturbadoras e falta de reconhecimento da profissão. As estratégias de coping mais utilizadas foram: planeamento, coping ativo, aceitação e autodistração. Conclusão: os estressores identificados relacionam-se principalmente a aspetos organizacionais e condições de trabalho, e as estratégias de coping escolhidas estão direcionadas para a resolução de problemas e melhoria do bemestar dos enfermeiros. Percentagem expressiva de enfermeiros apresentou níveis elevados de pressão e emoções deprimidas. Os resultados apresentados corroboram estudos anteriores que alertam para a importância do desenvolvimento de estratégias de prevenção dos níveis de estresse.

OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors

High salinity causes remarkable losses in rice productivity worldwide mainly because it inhibits growth and reduces grain yield. To cope with environmental changes, plants evolved several adaptive mechanisms, which involve the regulation of many stress-responsive genes. Among these, we have chosen OsRMC to study its transcriptional regulation in rice seedlings subjected to high salinity. Its transcription was highly induced by salt treatment and showed a stress-dose-dependent pattern. OsRMC encodes a receptor-like kinase described as a negative regulator of salt stress responses in rice. To investigate how OsRMC is regulated in response to high salinity, a salt-induced rice cDNA expression library was constructed and subsequently screened using the yeast one-hybrid system and the OsRMC promoter as bait. Thereby, two transcription factors (TFs), OsEREBP1 and OsEREBP2, belonging to the AP2/ERF family were identified. Both TFs were shown to bind to the same GCC-like DNA motif in OsRMC promoter and to negatively regulate its gene expression. The identified TFs were characterized regarding their gene expression under different abiotic stress conditions. This study revealed that OsEREBP1 transcript level is not significantly affected by salt, ABA or severe cold (5 °C) and is only slightly regulated by drought and moderate cold. On the other hand, the OsEREBP2 transcript level increased after cold, ABA, drought and high salinity treatments, indicating that OsEREBP2 may play a central role mediating the response to different abiotic stresses. Gene expression analysis in rice varieties with contrasting salt tolerance further suggests that OsEREBP2 is involved in salt stress response in rice.

Universidade como coping para lidar com o trabalho na assistência do mestrando enfermeiro

OBJETIVO: Analisar a relação entre os principais indícios de estresse, coping e estressores em mestrandos enfermeiros e o processo de elaboração da dissertação com sua inserção profissional. PROCEDIMENTOS METODOLÓGICOS: Pesquisa exploratória qualitativa de análise temática utilizando entrevistas individuais. A amostra de conveniência consistiu de 18 mestrandos e seis orientadores de uma universidade do Estado de São Paulo, em 2004. ANÁLISE DOS RESULTADOS: Apesar de o curso apresentar momentos estressantes, os mestrandos consideravam o estudo como oportunidade de abandonar a assistência para vir a lecionar. O aspecto mais prazeroso da pós-graduação foi dispor de um ambiente de reflexão das questões profissionais, reforçando a visão do mestrado como fuga e busca de suporte. O mestrado era visto pelo enfermeiro como fórum de legitimação do saber para conquistar o reconhecimento profissional não encontrado na assistência, onde se sentia desprestigiado, emergindo possivelmente deste contexto sua necessidade de "fugir" do hospital. CONCLUSÕES: Para os mestrandos de enfermagem o mestrado era menos estressante que suas atividades profissionais, consistindo em coping de fuga e busca de suporte para lidar com a prática assistencial.