Essays upon heredity and kindred biological problems, by Dr. August Weismann. Ed. by Edward B. Poulton, Selmar Schönland, and Arthur E. Shipley. Authorised translation.

v.1

Molecular cloning of a rat chromosome putative recombinogenic sequence homologous to the hepatitis B virus encapsidation signal.

Previously, we reported that a 61-bp subgenomic HBV DNA sequence (designated as 15AB, nt 1855-1915) is a hot spot for genomic recombination and that a cellular protein binding to 15AB may be the putative recombinogenic protein. In the present study, we established the existence of a 15AB-like sequence in human and rat chromosomal DNA by Southern blot analysis. The 15AB-like sequence isolated from the rat chromosome demonstrated a 80.9% identity with 5'-CCAAGCTGTGCCTTGGGTGGC-3', at 1872-1892 of the hepatitis B virus genome, thought to be the essential region for recombination. Interestingly, this 15AB-like sequence also contained the pentanucleotide motifs GCTGG and CCAGC as an inverted repeat, part of the chi known hot spot for recombination in Escherichia coli. Importantly, a portion of the 15AB-like sequence is homologous (82.1%, 23/28 bp) to break point clusters of the human promyelocytic leukemia (PML) gene, characterized by a translocation [t(15;17)], and to rearranged mouse DNA for the immunoglobulin kappa light chain. Moreover, 15AB and 15AB-like sequences have striking homologies (12/15 = 80.0% and 13/15 = 86.7%, respectively) to the consensus sequence for topoisomerase II. Our present results suggest that this 15AB-like sequence in the rat genome might be a recombinogenic candidate triggering genomic instability in carcinogenesis.

Immunogenicity of transgenic plant-derived hepatitis B surface antigen.

The focus of the Children's Vaccine Initiative is to encourage the discovery of technology that will make vaccines more readily available to developing countries. Our strategy has been to genetically engineer plants so that they can be used as inexpensive alternatives to fermentation systems for production of subunit antigens. In this paper we report on the immunological response elicited in vivo by using recombinant hepatitis B surface antigen (rHBsAg) purified from transgenic tobacco leaves. The anti-hepatitis B response to the tobacco-derived rHBsAg was qualitatively similar to that obtained by immunizing mice with yeast-derived rHBsAg (commercial vaccine). Additionally, T cells obtained from mice primed with the tobacco-derived rHBsAg could be stimulated in vitro by the tobacco-derived rHBsAg, yeast-derived rHBsAg, and by a synthetic peptide that represents part of the a determinant located in the S region (139-147) of HBsAg. Further support for the integrity of the T-cell epitope of the tobacco-derived rHBsAg was obtained by testing the ability of the primed T cells to proliferate in vitro after stimulation with a monoclonal anti-idiotype and an anti-idiotype-derived peptide, both of which mimic the group-specific a determinant of HBsAg. In total, we have conclusively demonstrated that both B- and T-cell epitopes of HBsAg are preserved when the antigen is expressed in a transgenic plant.

The placentation of the manatee (Trichechus latirostris) / by George B. Wislocki.

v.54:no.3 (1935)

Thaddeus Mason Harris' working documents regarding subject-based library catalog, ca. 1790

Thaddeus Mason Harris, who served as interim librarian of the Harvard College Library in 1787 and as its librarian from 1791 through 1793, is believed to have created these notes while helping compile the library's first printed subject-based catalog. The catalog, Catalogus Bibliothecae Harvardianae Cantabrigiae Nov-Anglorum, was published in 1790 and represented a significant change in approach to the cataloging of the library's collections, which had formerly been cataloged alphabetically. These documents, many of them on small scraps of paper, contain the titles and bibliographic information of books on a range of topics, from "Anatomici" to "Rhetorica."

Hādhā kitāb al-Hadīyah al-ḥamīdīyah fī al-lughah al-Kurdīyah

ijtahada bi-jamʻihi Yūsuf Ḍiyāʼ al-Dīn Pashā al-Khālidī al-Maqdisī.

Maʻmūlāt-i Maẓharīyah va Maḥbūb al-ʻārifīn

[Naʻīm Allāh al-Ḥanafī al-Majdawī Bahrāychī].

Hādhā kitāb Qiṣaṣ al-ʻulamāʼ

min taʼlīfāt al-ʻālim al-fāḍil al-kāmil nukhbat al-ʻulamāʼ wa-al-mujtahidīn al-Mīrzā Muḥammad ibn Sulaymān al-Tunukābunī.

Hādhā kitāb al-Farāʼid al-raḍwīyah

min taʼlīfāt Mīrzā Muḥammad ibn Sulaymān al-Tunukābunī.