OB protein binds specifically to the choroid plexus of mice and rats.

Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissue from lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats were probed to identify potential central OB protein-binding sites. We report here that recombinant OB protein binds specifically to the choroid plexus. The binding of OB protein (either radiolabeled or the alkaline phosphatase-OB fusion protein) and its displacement by unlabeled OB protein was similar in lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats. These findings suggest that OB protein binds with high affinity to a specific receptor in the choroid plexus. After binding to the choroid plexus receptor, OB protein may then be transported across the blood-brain barrier into the cerebrospinal fluid. Alternatively, binding of OB protein to a specific receptor in the choroid plexus may activate afferent neural inputs to the neural network that regulates feeding behavior and energy balance or may result in the clearance or degradation of OB protein. The identification of the choroid plexus as a brain binding site for OB protein will provide the basis for the construction of expression libraries and facilitate the rapid cloning of the choroid plexus OB receptor.

Lonnie Holley exploration and analysis of his artworks in two settings : the yard and the museum

The contemporary artist, Lonnie Holley, creates assemblage sculptures using found objects that he then places in his multi-layered yard art environment. With the rise in prestige of folk art, many art galleries and museums have displayed the works of Holley, removing them from the yard art environment and placing them in the gallery setting. This paper addresses how meaning changes when the context of Holley’s artworks changes.

La théorie sociale de la connaissance et la gestion du patrimoine documentaire à l’ère numérique

Les institutions de mémoire et de savoir (par exemple, les bibliothèques, les archives, les musées) font face à des défis importants dans leurs responsabilités d’assurer la pérennité du patrimoine documentaire à l’ère numérique. Citons la surabondance de l’information numérique, la possibilité de production, théoriquement illimitée, offerte aux individus comme aux groupes sociaux, les limites dans les capacités de stockage et de diffusion de l’information numérique qui sont à la disposition des institutions mandataires du patrimoine documentaire. De plus, il est apparu que les approches et les méthodes utilisées pour identifier, gérer, préserver et diffuser le patrimoine documentaire de la société canadienne dans un environnement analogique n’étaient transférables à un environnement numérique. Nous suggérons que la théorie sociale de la connaissance peut servir de base à une réflexion portant sur le développement d’une politique publique qui viserait à encadrer l’identification, la sélection, la gestion et la préservation du patrimoine documentaire d’une société à l'ère numérique. Nous définissons la problématique puis proposons des réponses à travers trois articles scientifiques. Les résultats indiquent que les connaissances et les pratiques professionnelles utilisées demeurent persistantes et limitent la formulation et l'application de nouveaux cadres théoriques, de politiques administratives et de techniques associés à l'identification et la sélection du patrimoine documentaire. Cette recherche propose un cadre conceptuel qui permet de développer des politiques publiques sur le patrimoine documentaire du Canada.

Postsecondary education and the new workforce /

"PLLI 96-8005"--P. [4] of cover.

Libraries enhance student learning : a guidebook of innovative library programs for youth /

Mode of access: Internet.

Decomposition of residential and light commercial solid waste in test lysimeters /

"Contract number 68-03-0315."

Codisposal of municipal solid waste and sewage sludge : an analysis of constraints /

"Contract no. 68-01-4427"--Verso of t.p.

Source separation collection and processing equipment : final report.

"January 1981"--Cover.

Cooperation of library and school.

Extract from the Normal Seminar, v. 1, no. 1, March, 1904.

Learning and earning : analysis of HEA Title II-B Graduate Library Fellowship Program recipients, fiscal years 1985-1991 /

"PLLI 97-8014"--P. [4] of cover.

Wings of hope : the U.S. Air Force and humanitarian airlift operations /

Mode of access: Internet.

Issues and innovations in electronic theses and dissertations /

"October 1998"--T.p. verso.

Realizing the potential : improving postsecondary teaching, learning and assessment /

"Grant R117G10037"--T.p. verso.

Issues and innovations in distance learning /

"September 1998"--T.p. verso.

Small modular incinerator systems with heat recovery : a technical, environmental, and economic evaluation /

November 1979.