Resposta de plantas daninhas ao óleo fúsel aplicado em pós-emergência inicial e tardia

This work aimed to study weed response to fusel oil rate applied at early and late post-emergence. The following species were studied at late and early post-emergence: Ipomoea hederifolia, Ipomoea quamoclit, Euphorbia heterophylla, Digitaria spp., Cenchrus echinatus and Panicum maximum.. The experiment was arranged in a completely randomized design with six treatments and four replications. Fusel oil, an alcohol distillation byproduct, was applied at rates of 50, 125, 250, 375 and 500 L ha(-1), plus control without application. The plots were constituted by polyethylene containers with 3 L capacity, filled with topsoil land from a fallow area. Visual symptoms of intoxication were verified at 7 and 30 days after application (DAA) and dry weight at 30 DAA. The variables were submitted to variance analysis according to the design, adjusting the data to obtain the dose-response curve by polynomial regression. The studied species were susceptible only to the application of 500 L ha(-1) of fusel oil at early or late plant post-emergence. The species Digitaria spp. was susceptible and E. heterophylla tolerant to fusel oil applied at early post-emergence, while the other species were moderately tolerant. E. heterophylla was susceptible, Digitaria spp., C. echinatus and P. maximum moderately tolerant and I. hederifolia and I. quamoclit tolerant to fusel oil applied at late post-emergence.

Histopathological evaluation in experimental envenomation of dogs with Crotalus durissus terrificus Venom

The present work evaluated histopathological aspects in experimental envenomation of dogs with Crotalus durissus terrificus venom. Twenty-eight mixed breed adult dogs were divided into three groups of seven animals each: Group I only venom; Group II - venom + 50ml antiophidic serum + fluid therapy; Group III venom + 50ml antiophidic serum + fluid therapy + urine alkalization. Lyophilized venom of Crotalus durissus terrificus was reconstituted in saline solution and inoculated subcutaneously at the dose of 1mg/kg body weight. Three animals of each group were subjected to euthanasia, and their muscular tissue, brain, spleen, kidneys, heart, lungs, stomach, small and large intestines, and popliteal lymph node fragments were collected for histopathological evaluation. There was myonecrosis in the inoculated limb, renal tubular degeneration, lymphoid hyperplasia of spleen, and unspecific reactive hepatitis. These results show the antigenicity and action of the venom on the immune system.

Efeitos tóxicos de surfactantes fitossanitários para o peixe mato grosso (Hyphessobrycon eques)

Toxic effects of phytosanitary surfactants for jewel tetra (Hyphessobrycon eques). Surfactants are amphipatic molecules that reduce the surface tension of water and make up the inert components of pesticide formulations. Thus, the objectives of this study were: to estimate the lethal concentration (LC(I)50;96h); classify and evaluate water quality during testing of the following surfactants: Agral[registered trademark], Aterbane[registered trademark]BR, Ag-bem[registered trademark], Energic[registered trademark], Fixade[registered trademark] and Gotafix[registered trademark] for jewel tetra (Hyphessobrycon eques); and the signs of intoxication in the animals. For this, the fish were acclimated for ten days in the bioassay room. The animals were exposed to the surfactants in an entirely randomized design with three replications. The LC(I)(50;96h) of surfactant Agral[registered trademark] was 3.29 mg L-1; Aterbane[registered trademark] BR 8.21 mg L-1; Energic[registered trademark] 2.34 mg L-1; Gotafix[registered trademark] 4.37 mg L-1; Fixade[registered trademark] 3.38 mg L-1; and Ag-bem[registered trademark] 34.95 mg L-1. The variables of water quality were unchanged. The fish showed an increase in the opercular beating after exposure; 4 and 24 hours, loss of gasping ability; 48 hours, slow opercular beating; and 72 and 96 hours later, recovery. The surfactants Energic[registered trademark], Agral[registered trademark], Gotafix[registered trademark], Aterbane[registered trademark] BR and Fixade[registered trademark] can be classified as moderately toxics, and Ag-Bem[registered trademark] as slightly toxic for H. eques; this organism shows similar intoxication signs for all surfactants.

Hepatic lesions in protein-deficient adult rats

Four groups of 10 young adult Wistar male rats were fed ad libitum on a protein-free diet for periods of 7, 28, 56 and 84 days. Control groups were fed on a 20% casein diet. Food intake and body weights of rats were registered. Plasma protein levels and liver weight and fat content were determined. Sections of the caudate lobe were studied histologically. Fatty changes were classified in three grades. Protein-deficient rats exhibited loss of body weight and had low levels of plasma protein concentration. Liver lost weight after 7 days of protein deficiency; there was a gradual reduction in liver weight as periods of protein deprivation were longer. After 7 days, liver fat concentration was not significantly higher than in the respective control group; it was significantly higher in all the other malnourished animals, As periods of protein deprivation were longer, fatty changes became more severe. Other hepatic lesions were found in 5 of the 10 rats submitted to the longest period of protein deficiency. One of the rats showed a diffuse cellular atrophy, 2 animals showed an extensive haemorrhagic necrosis, another showed a focal area of reticulum collapse and the last exhibited a distortion of the normal architecture of the liver due to diffuse reticulum collapse and early nodular regeneration; these 2 last rats showed early fibrosis in portal areas. The findings suggest that other deficiencies may complicate the protein deficiency when rats are given a protein-free diet over prolonged periods. Even if the protein-deficient diet has protective nutrients, it may be that, when rats eat less food, as occurs in prolonged experiments deficiency of one or all of these elements can occur, depending on their relative amount in diet.

EXPOSICAO DE CIRURGIOES-DENTISTAS AO MERCURIO

The professional exposure of dental surgeons to mercury has attracted a great deal of attention from governmental organs and institutions in different countries. Considering that the normal ratio is 10 μg/1000 ml of urine, it was verified from tests that 72.7% of the professionals working in the dental practice in the city of Araraquara (SP) Brazil, show mercury levels above this limit. These results demonstrated the almost complete unawareness of many dentists to mercury and, consequently, precautionary measures were almost nonexistent.

ACAO ANTIARRITMICA DA METOCLOPRAMIDA (PLASIL®) EM INTOXICACAO DIGITALICA EXPERIMENTAL. COMPARACAO COM A DIFENIL-HIDANTOINA

The antiarrhythmic action of metoclopramide in cardiac arrhythmias caused by toxic doses of desacetyl-lanatoside C and digitoxin was studied in dogs. A comparison was made between the efficacy of metoclopramide and diphenylhydantoin in arrhythmias caused by digitoxin. The results indicate that metoclopramide antagonized the arrhythmias induced by desacetyl-lanatoside C, while causing a transitory reversion of those induced by digitoxin in most of the experiments. Diphenylhydantoin failed to show an arrhythmic effect in the majority of the tested animals.

The effect of Ketoconazole on experimental paracoccidioidomycosis in the Syrian hamster: immunological and histopathological study

The effect of Ketoconazole (KTZ) on the hamster experimental intratesticular paracoccidioidomycosis was studied employing different treatment schedules. KTZ long course treatment beginning at an early stage of the infection was effective in preventing fungal proliferation, dissemination to lymph nodes, spleen and kidneys, and in maintaining low levels of humoral and cellular specific immune responses. KTZ short course treatment starting at an advanced stage of disease resulted in a more severe histopathological picture without significant changes in the immunological profile. The drug prolonged the life span of hamsters infected with Paracoccidioides brasiliensis, but did not prevent mortality. Toxic necrosis of the bone marrow occurred in normal animals receiving 120 mg/kg/day of KTZ but with lower doses no morphologic alterations were observed in heart, lungs, kidneys, adrenals, spleen, liver, intestine or bone marrow. © 1984 Dr W. Junk Publishers.

AVALIACAO DE BERGAPTENO 3CH E 6CH NA PROTECAO DE PEPINEIROS (CUCUMIS SATIVUS) CONTRA INTOXICACOES POR ESSA MESMA SUBSTANCIA

Authors report trial with bergapten using homeopathic preparations and the protection of cucumber seedlings (Cucumis sativus) against intoxications by the same substance and assess the positive results obtained.

Épocas de consórcio de alho com beterraba perante três manejos do mato sobre a produtividade e o lucro

The research was carried out with intercropped cultivation of garlic and beet, in Caçador, SC, Brazil. Four seeding epochs for beet (0, 15, 30 and 45 days after garlic planting) and three management systems for weeds (with herbicides, without control and with frequent weeding) were tested. It was hoped to determine the effects of this intercropping, in time and space, seeking the benefits in .weed control, efficient land use, productivity, commercial quality, and profitability. The randomized block design was in split-plots. The epochs represented the plots, and the management, the subplots, with four replicates. Napromide at 750 g/ha did not cause symptoms of intoxication in the garlic plants, nor in the beet. All the treatments in epochs at the initiation of intercropping, in three management systems for weeds, presented efficient land use values greater than 1. The profitability of the intercropping of garlic and beet only became evident in the management involving frequent weeding; in all epochs, profits were greater than for the respective epochs of monoculture of beet and monoculture of garlic.

Evolucao materno-infantil da contaminacao por mercurio: Estudo em duas populacoes indigenas brasileiras

Considering the severity of Hg intoxication to human health and the frequent use of the metal in gold prespection on Brazilian Amazonia, an extensive project in being developed in order to quantify such conditions in two indigenous Kayapo populations, inhabitants of South of Para State, Gorotire and Djudjetikitire. This paper reports evolution of this contamination on mothers and babies. During puerperium, it was registered both an important decline on dosimetric values observed before in pregnancy and the occurrence of expressive levels in the babies. Such data are discussed based on contemporary medical-biologic conceptions, since it raises conceptual review of the matter.

Study of the presence of the spores of Clostridium botulinum in honey in Brazil

The isolation of Clostridium botulinum from honey samples is described. Botulism is characterized as an intoxication provoked by ingestion of contaminated foods with this toxin. Infant botulism happens by the ingestion of spores of C. botulinum together with food that in special conditions of the intestinal tract, such as those present in babies of less than 1 year old, will allow the germination and colonization of the intestine with production and absorption of botulinic toxin. The samples were subjected to dilution and to a thermal shock and cultivated in modified CMM (Difco). Cultures were subjected to Gram smears and toxicity tests in mice. The toxic cultures were purified in RFCA (Oxoid) plates and incubated in anaerobic jars. Positive samples were typed using the mouse assay neutralization test. From the 85 honey samples analyzed, six were positive for C. botulinum (7.06%), and identified as producers of type A, B, and D toxins.