Characterization of Hypoxia: Topic I Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico

Nutrient overenrichment from human activities is one of the major stresses affecting coastal ecosystems. There is increasing concern in many areas around the world that an oversupply of nutrients from multiple sources is having pervasive ecological effects on shallow coastal and estuarine areas. These effects include reduced light penetration, loss of aquatic habitat, harmfid algal blooms, a decrease in dissolved oxygen (or hypoxia), and impacts on living resources. The largest zone of oxygen-depleted coastal waters in the United States, and the entire western Atlantic Ocean, is found in the northern Gulf of Mexico on the Louisiana-Texas continental shelf. This zone is influenced by the freshwater discharge and nutrient flux of the Mississippi River system. This report describes the seasonal, interannual, and long-term variability in hypoxia in the northern Gulf of Mexico and its relationship to nutrient loading. It also documents the relative roles of natural and human-induced factors in determining the size and duration of the hypoxic zone.

Baseline assessment of Guánica Bay, Puerto Rico in support of watershed restoration

Guánica Bay is a major estuary on the southwest coast of Puerto Rico. Significant coral reef ecosystems are present outside the bay. These valuable habitats may be impacted by transport of sediments, nutrients and contaminants from the watershed, through the bay and into the offshore waters. The National Oceanic and Atmospheric Administration’s (NOAA) National Centers for Coastal Ocean Science (NCCOS), in consultation with local and regional experts, conducted an interdisciplinary assessment of coral reef ecosystems, contaminants, sedimentation rates and nutrient distribution patterns in and around Guánica Bay. This work was conducted using many of the same protocols as ongoing monitoring work underway elsewhere in the U.S. Caribbean and has enabled comparisons among coral reef ecosystems between this study and other locations in the region. This characterization of Guánica marine ecosystems establishes benchmark conditions that can be used for comparative documentation of future change, including possible negative outcomes due to future land use change, or improvement in environmental conditions arising from management actions. This report is organized into six chapters that represent a suite of interrelated studies. Chapter 1 provides a short introduction to the study area. Chapter 2 is focused on biogeographic assessments and benthic mapping of the study area, including new surveys of fish, marine debris and reef communities on hardbottom habitats in the study area. Chapter 3 quantifies the distribution and magnitude of a suite of contaminants (e.g., heavy metals, PAHs, PCBs, pesticides) in both surface sediments and coral tissues. Chapter 4 presents results of sedimentation measurements in and outside of the bay. Chapter 5 examines the distribution of nutrients in in the bay, offshore from the bay and in the watershed. Chapter 6 is a brief summary discussion that highlights key findings of the entire suite of studies.

A geo-referenced benthic habitat survey in support of natural resource management: Port Graham Bay, Alaska

The impact of recent changes in climate on the arctic environment and its ecosystems appear to have a dramatic affect on natural populations (National Research Council Committee on the Bering Sea Ecosystem 1996) and pose a serious threat to the continuity of indigenous arctic cultures that are dependent on natural resources for subsistence (Peterson D. L., Johnson 1995). In the northeast Pacific, winter storms have intensified and shifted southward causing fundamental changes in sea surface temperature patterns (Beamish 1993, Francis et al. 1998). Since the mid 1970’s surface waters of the central basin of the Gulf of Alaska (GOA) have warmed and freshened with a consequent increase in stratification and reduced winter entrainment of nutrients (Stabeno et al. 2004). Such physical changes in the structure of the ocean can rapidly affect lower trophic levels and indirectly affect fish and marine mammal populations through impacts on their prey (Benson and Trites 2002). Alaskan natives expect continued and perhaps accelerating changes in resources due to global warming (DFO 2006).and want to develop strategies to cope with their changing environment.

Successful inoculation of Artemia and production of cysts in the coastal saltpans of Bangladesh II

Bangladesh has no naturally occurring Artemia, and all the growing shrimp hatcheries of the country depend entirely on import of cysts from foreign countries. Following successful inoculation of Artemia and production of cysts for the first time in this country in a coastal saltpan (at Chanua, Banskhali) by the senior author (in 1989-90), a similar second attempt was made under this programme in a saltpan (1000 m super(2)) of Demoshia, Chakaria, Cox's Bazar, Bangladesh between January and April 1992. A total of 1639.9 g (dry weight) of cysts (i.e. 5.46 kg DW/ha/month) have been produced using the Red Jungle Brand, whereas the previous attempt obtained 517 g of cysts (i.e. 2.07 kg DW/ha/month) using the Great Salt Lake Brand.

Growth of juvenile freshwater prawn Macro brachium mslcolmsonii fed with various protein diets containing different biowastes

An experiment was conducted with juvenile prawns Macrobrachium malcolmso11ii, (0:76± 0.01 ro 0.94-::tO.Ol g) w evaluate various protein source diets. Six diets containing 20%, 25%, 30%, 35%, 40%,and 45% of crude protein were formulated, and fed to prawns in the form of pellet to evaluate their suitability. The experiment was designed for 60 days and sampling was made at every 15 days interval. At the end of the study period growth, feed conversion ration (FCR) specific growth rate (SGR), feed efficiency and survival were determined for prawns in each dietary treatment. Among the above five feeds poor FCR and higher weight gain observed in 35% protein diet (B-4). Similarly specific growth rate and feed efficiency are also highest with diet containing 35% protein. The dietary protein levels above 35% exerts a decrease in growth of prawn was observed in the present study. The feed efficiency ratio and protein efficiency ratio decreased with the increased dietary protein levels. It is concluded that 35% protein diet could be suitable with optimum protein supply for Macrobrachium malcolmsonii Therefore, above and below this 35% protein level in the formulated feed leads to metabolic stress which lowers the conversion efficiency and wastage of nutrients.

Seasonal fluctuation in primary production in relation to the physicochemical parameters of two weed-infested ponds of Kalyani, West Bengal

Kalyani lake (P sub(1)), a weed infested recreational water body and a weed chocked derelict water body (P sub(2)) in the heart of Kalyani city of West Bengal were studied for a period of one year for their primary productivity and other physicochemical parameters. Very low primary productivity (GPP=360-1237mg C m super(-2) d super(-1); NPP=157-787 mg C m super(-2) d super(-1)) was recorded in P sub(2) in spite of having a high concentration of nutrients (PO sub(4)–P=0.052-0.260mg l super(- 1); NO sub(3)-N=0.110-0.412mg l super(-1)). On the other hand, moderate primary productivity (GPP=1687-3195mg C m super(-2) d super(-1); NPP=900-2700mg C m super(-2) d super(-1)) was found in P sub(1) with comparatively low range of nutrients (P0 sub(4)-P =0.010-0.058mg l super(-1); NO sub(3)-N=0.032-0.118mg l super(-1)). After studying the other physicochemical parameters (temperature, transparency, dissolved oxygen, free carbon dioxide, pH, alkalinity and macrophytic biomass), it was found that the overall hydro-biological conditions of the weed-chocked derelict water body (P sub(2)) is not congenial for biological production as compared to Kalyani Lake (P sub(1)). Kalyani Lake may be used for fish culture with proper management practices.

Fish-makhana (Euryale salisb) integration: a case study of sustainable aquafarming system in north Bihar

The paper deals with a technique to synchronize two crops, fish and makhana (Euryale ferox Salisb) in a pond. In such eco-friendly integration both crops are mutually benefited. Decomposed plant parts of makhana crop form organic matter that releases nutrients in the water to enhance plankton population. Organic detritus not only acts as food for bottom dwelling fishes (mrigal and common carp) but also provides a suitable substratum for the growth of zooplankton, insect larvae, nematodes and gastropods. Fishes contribute to the control of makhana pests. Their faecal matter acts as organic manure for makhana crop. Plankton population fluctuated between 1260 u/l to 4030 u/l in the control pond and 1630 u/l to 4722 u/l in the experimental pond. During the grand growth period of makhana crop (April to July) the dissolved oxygen content fluctuated between 5.02 mg/l to 6.68 mg/l in the covered areas and 6.04 mg/l to 6.92 mg/l in uncovered areas. Makhana leaves acting as blanket barrier over the water surface brought down the D.O. content in the covered areas of the pond. Free CO sub(2) content showed wider fluctuation in the experimental pond (25.2 mg/l to 30.9 mg/l) than in the control pond (25.1 mg/l to 28.6 mg/l). This could be due to decomposition of plant parts of the presiding crop lying as debris at the pond bottom. Autochthonous supply of nutrients enhanced the content of nitrogen, phosphorous and organic carbon in the soil of experimental pond. The experimental pond covering an area of 0.40 ha yielded 852 kg fish and 200 kg pops whereas the control pond covering the same area produced 777 kg fish only. The net profit per ha came out to be Rs.1,04,700 and Rs. 66,200 in integrated and non-integrated system respectively. Owing to crop diversification, the present integrated system was found to be more viable than the non-integrated system in terms of production and net profit.

Effect of feed quality restriction followed by realimentation on growth, nutrient utilization, biochemical changes and haematological profiles of Indian major carp, rohu (Labeo rohita H.)

To investigate the effect of protein restriction with subsequent re-alimentation on nutrient utilization, hematological and biochemical changes of Indian major carp, Rohu (Labeo rohita H.), 150 acclimatized Rohu fingerlings (average 20.74 ± 0.13 g) divided into five experimental groups (30 fingerlings in each groups with three replications with 10 fingerlings in each) for experimental trial of 90 days using completely randomized design. Control group (T sub(CPR)) was fed with feed having 30% crude protein at 3% of body weight for 90 days trial period. Other experimental groups T sub(1PR) was alternatively 3 days fed with feed having 20% CP and 30% CP at 3% of body weight, T sub(2PR) was alternatively 7 days fed with feed having 20% CP and 30% CP at 3% of body weight, T sub(3PR) was alternatively 15 days fed with feed having 20% CP and 30% CP at 3% of body weight and T sub(4PR) was alternatively 25 days fed with feed having 20% CP and 30% CP at 3% of body weight during 90 days trial period with daily ration in two equal halves at morning and afternoon. It was noticed that retention of different nutrients was almost similar among all treatment groups indicated improvement of digestibility of nutrients might not be the mechanisms for recovery growth in carps. Increased percent feed intake of body weight (hyperphagia) (4.14 ± 0.30 or 4.94 ± 0.46 and 3.33 ± 0.29), improved specific growth rate (1.86 ± 0.09 or 2.26 ± 0.05 and 1.43 ± 0.01), absolute growth rate (1.57 ± 0.08 or 1.84 ± 0.18 and 1.36 ± 0.12), protein efficiency ratio (1.19 ± 0.11 or1.16 ± 0.12 and 1.05 ± 0.09) were the important mechanism showing better performance index (21.60 ± 1.09 or 23.80 ± 0.21 and 19.45 ± 0.37) through which the experimental groups which were protein restricted and re-alimented at 3 or 7 days alternatively during 90 days trial period could able to compensate the growth retardation and to catch up the final body weight of control (128.68 ± 11.53 g/f) but other experimental groups failed to compensate during 90 days trial period. Result of the present study indicated that deprived fish i.e., fish received alternate 3 or 7 days protein restriction and re-alimentation showed recovery growth had still lower values of Hb (10.21 ± 0.02, and 9.88 ± 0.04 g/dl), hematocrit value (30.62 ± 0.05 and 26.64 ± 0.11%), total erythrocytic count (3.40 ± 0.01 and 3.29 ± 0.01 X10super(6) mm³), plasma glucose (126.93 ± 0.20 and 126.67 ± 0.05 mg/dl), total plasma lipid (1.04 ± 0.01 and 1.02 ± 0.01 g/dl) and liver glycogen (290.10 ± 0.80 and 288.99 ± 0.95 mg/kg) in comparison to control (10.56 ± 0.08 g/dl, 31.68 ± 0.24%, 3.52 ± 0.03 X10super(6) mm³, 128.23 ± 0.25 mg/dl, 1.07 ± 0.01g/dl and 292.00 ± 0.23 mg/kg) at the end of 90 days trial but total plasma protein in deprived group was compensated with advancement of trial period. All hematological and biochemical parameters studied were proportionately lowered in the experimental group got higher degree of deprivation. These findings suggested that with the increase of trial length complete compensation of hematological and biochemical profiles of rohu might be achieved. The results indicated that the implementation of alternative 7 days low and high protein diet feeding during aquaculture of carps could make economize the operation through minimizing the feed input cost.

Assessing potential of production in Lake Choghakhor

Phytoplankton productivity is the common and important factor being considered in determining the overall status of a given body of water. This is because they are found at the base of an energy or food chain, being the basic source of primary food in a given aquatic system. Hence, information on their contribution is essential in indicating how much biomass energy will be available to all other living resources in the system. Though the primary productivity of shallow lakes is characterized by mixed populations of phytoplankton and submersed aquatic vegetation in the open water. Lake Choghakhor, is a shallow lake, located in Chaharmahal-Bakhtiyari Province. This lake is the most important ecosystem in the region especially for waterfowl populations, has a recreational value and supports tourism and fisheries. During last decade Choghakhor has been influenced by some man-made impacts such as water level fluctuation, agricultural discharge and fish (Cyprinids) introduction causing a serious problem in its trophic states. So water quality for physical, chemical and biological was monitored in five sampling stations, from April 2003 to March 2004. As biological parameters we studied phytoplankton, epiphytic algae, and zooplankton and macrobenthose community structure. Chlorophyll a content for phytoplankton and epiphytes was measured to estimate production of these groups (biomass over time). Also we determined biomasses of submersed macrophytes and macrobenthose and primary production of phytoplankton (dark and light bottles technique) to estimate fish production. The results of this study showed Lake Choghakhor did not undergo stable thermal and oxygen stratification, and the lake water was mixed throughout the study (the lake mixing regime is polymictic). Now submerged plants especially Myriophyllum spicatum has covered almost the entire lake and dense macrophyte beds (Polygonom amphibium), located on the east southern end of the lake appear to act as a sink for these nutrients. Lake Choghakhor appeared to be in a macrophyte dominated clear water state with low TP (annual mean: 24± 15μg.l-1) and chlorophyll a (annual mean: 3±1.28μg.l-1) concentrations and very high Secchi depth. The grazing pressure of dominant pelagic filtering zooplankton Daphnia longespina did not seem to be significant in determining the low phytoplankton crop expressed as chlorophyll a. We expect that sequestering of nutrients by submerged plants and associated epiphytes are the dominant stabilizing mechanisms suppressing the phytoplankton crop of Lake Choghakhor.

Phytoplankton biomass in relation to nutrients and zooplankton in thirty subtropical lakes adjacent to the Yangtze River, China

A total of 30 shallow lakes, located along the middle and lower reaches of the Yangtze River, were studied to assess the relative importance of nutrients and zooplankton biomass in determining the phytoplankton biomass in subtropical China. Zooplankton biomass and nutrients both varied greatly in these lakes. Factor analysis and multiple linear regression showed that phytoplankton biomass was positively correlated with TN, NH4+, NO3- and TP, while it did not show any negative relationship to zooplankton biomass. Meanwhile, the phytoplankton biomass showed contrary relationships to the mass ratio of TN/TP in spring and summer, suggesting that in nutrient-richer lakes the dominant phytoplankton species have different preferences for TN/TP ratio. The insignificant top-down control of phytoplankton biomass may be attributed to the dominance of small-sized crustaceans and low crustacean biomass resulting from cyanobacterial dominance and planktivorous fish predation as well as other factors. Thus, it is likely that nutrients were more important than zooplankton biomass in explaining the total variance of phytoplaDkton biomass in these subtropical lakes.

Effects of the phytoplanktivorous silver carp (Hypophthalmichthys molitrixon) on plankton and the hepatotoxic microcystins in an enclosure experiment in a eutrophic lake, Lake Shichahal in Beijing

The responses of nutrients, water transparency, zooplankton, phytoplankton and microcystins to a gradient of silver carp biomass (0, 18, 55, 110 g/m(3)) were assessed using enclosures in Lake Shichahai (Beijing). Picophytoplankton biomass increased with increasing fish stocking density (r=0.64, p=0.09). Silver carp significantly depressed zooplankton biomass, and thus, zooplankton grazing was too low to control phytoplankton. Intracellular microcystin (MC) content in the enclosures was correlated only to Microcystis biomass in the present study. Microcystis spp. biomass and intracellular microcystins content were much higher in lake water than those of enclosures with and without stocking fish. Stocking of silver carp could be an appropriate in highly productive Lake Shichahai, which naturally lacks of large cladoceran zooplankton. A fish stocking density of 55 g/m(3) was most efficient at controlling Microcystis blooms and increasing water clarity. Mean extracellular MC concentration in the lake water was almost the same with that of the enclosures with fish. (c) 2006 Elsevier B.V. All rights reserved.

Studies on the effects of silver carp (Hypophthalmichthys molitrix) on the Phytoplankton in a shallow hypereutrophic lake through an enclosure experiment

The responses of nutrients, water transparency, zooplankton and phytoplankton to a gradient of silver carp biomass were assessed using enclosure methods. The gradient of four silver carp biomass levels was set as follows: 0, 116, 176 and 316 g m(-2). Nutrients did not show any statistically significant differences among the treatments. An Outburst of Daphnia only occurred in fishless enclosures where phytoplankton biomass was the lowest and water clarity significantly increased. While among fish enclosures, the small-sized Moina micrura dominated throughout the experiment and both zooplankton and phytoplankton biomasses decreased with increased fish biomass. No large colonial cyanobacterial blooms occurred in the fishless enclosures as predicted. This might be due to low water temperature. short experiment time and the occurrence of large bodied Daphnia in our experiment. Cryptophyta was the most dominant group in most of the enclosures and the lake water throughout the experiment. The fishless enclosure had much lower proportion of Cyanophyta but higher proportion of Trachelomonas sp.

Budgets and dynamics of nitrogen and phosphorus in a shallow, hypereutrophic lake in China

Budgets and dynamics of nitrogen and phosphorus in Lake Donghu were investigated from Oct. 1997 to Sept. 1999. The water residence time was estimated to be 89 days in 1997-1998 and 124 days in 1998-1999. The total external loadings were 53 g N m(-2) yr(-1) and 3.2 g P m(-2) yr(-1) in 1997-1998, and 42 g N m(-2) yr(-1) and 3.1 g P m(-2) yr(-1) in 1998-1999. On average, about 80% of nitrogen and phosphorus input was from sewage outlets, while the rest was from land runoff and precipitation. Ammonium ion was the most abundant form of inorganic nitrogen in the sewage. The nutrient output was mainly through water outflow and fish catch. The percentages of nutrients in fish were estimated to be 7.8%-11.2% for nitrogen and 47.6%- 49.6% for phosphorus. Lake Donghu has a very high nutrient retention (63% for nitrogen and 79% for phosphorus) mainly due to its closure and long water residence time. Sedimentation is an important nutrient retention mechanism in this lake. Using mass balance method, we estimated that denitrification of Lake Donghu involves about 50% of the retained nitrogen. Lake Donghu is rich in inorganic nitrogen and phosphorus and showed great seasonal variation.

Methods for physical separation and characterization of soil organic matter fractions

Turnover of soil organic matter (SOM) is coupled to the cycling of nutrients in soil through the activity of soil microorganisms. Biological availability of organic substrate in soil is related to the chemical quality of the organic material and to its degree of physical protection. SOM fractions can provide information on the turnover of organic matter (OM), provided the fractions can be related to functional or structural components in soil. Ultrasonication is commonly used to disrupt the soil structure prior to physical fractionation according to particle size, but may cause redistribution of OM among size fractions. The presence of mineral particles in size fractions can complicate estimations of OM turnover time within the fractions. Densiometric separation allows one to physically separate OM found within a specific size class from the heavier-density mineral particles. Nutrient contents and mineralization potential were determined for discrete size/density OM fractions isolated from within the macroaggregate structure of cultivated grassland soils. Eighteen percent of the total soil C and 25% of the total soil N in no-till soil was associated with fine-silt size particles having a density of 2.07-2.21 g/cm3 isolated from inside macroaggregates (enriched labile fraction or ELF). The amount of C and N sequestered in the ELF fraction decreased as the intensity of tillage increased. The specific rate of mineralization (mug net mineral N/mug total N in the fraction) for macroaggregate-derived ELF was not different for the three tillage treatments but was greater than for intact macroaggregates. The methods described here have improved our ability to quantitatively estimate SOM fractions, which in turn has increased our understanding of SOM dynamics in cultivated grassland systems.

Spatial variability in water quality and surface sediment diatom assemblages in a complex lake basin,lake of the woods,ontario,canada

Lake of the Woods (LOW) is an international waterbody spanning the Canadian provinces of Ontario and Manitoba, and the U.S. state of Minnesota. In recent years, there has been a perception that water quality has deteriorated in northern regions of the lake, with all increase in the frequency and intensity of toxin-producing cyanobacterial blooms. However, given the lack of long-term data these trends are difficult to verify. As a first step, we examine spatial and seasonal patterns in water quality in this highly complex lake on the Canadian Shield. Further, we examine surface sediment diatom assemblages across multiple sites to determine if they track within-take differences in environmental conditions. Our results show that there are significant spatial patterns in water quality in LOW. Principal Component Analysis divides the lake into three geographic zones based primarily on algal nutrients (i.e., total phosphorus, TP), with the highest concentrations at sites proximal to Rainy River. This variation is closely tracked by sedimentary diatom assemblages, with [TP] explaining 43% of the variation in diatom assemblages across sites. The close correlation between water quality and the surface sediment diatom record indicate that paleoecological models could be used to provide data on the relative importance of natural and anthropogenic sources of nutrients to the lake.