Muscle transcriptome adaptations with mild eccentric ergometer exercise

The muscle has a wide range of possibilities to adapt its phenotype. Repetitive submaximal concentric exercise (i.e., shortening contractions) mainly leads to adaptations of muscle oxidative metabolism and endurance while eccentric exercise (i.e., lengthening contractions) results in muscle growth and gain of muscle strength. Modified gene expression is believed to mediate these exercise-specific muscle adjustments. In the present study, early alterations of the gene expression signature were monitored by a muscle-specific microarray. Transcript profiling was performed on muscle biopsies of vastus lateralis obtained from six male subjects before and in a 24-h time course after a single bout of mild eccentric ergometer exercise. The eccentric exercise consisted of 15 min of eccentric cycling at 50% of the individual maximal concentric power output leading to muscle soreness (5.9 on a 0-10 visual analogue scale) and limited muscle damage (1.7-fold elevated creatine kinase activity). Muscle impairment was highlighted by a transient reduction in jumping height after the eccentric exercise. On the gene expression level, we observed a general early downregulation of detected transcripts, followed by a slow recovery close to the control values within the first 24 h post exercise. Only very few regulatory factors were increased. This expression signature is different from the signature of a previously published metabolic response after an intensive endurance-type concentric exercise as well as after maximal eccentric exercise. This is the first description of the time course of changes in gene expression as a consequence of a mild eccentric stimulus.

[Arterial pressure curve and fluid status.]

Fluid optimization is a major contributor to improved outcome in patients. Unfortunately, anesthesiologists are often in doubt whether an additional fluid bolus will improve the hemodynamics of the patient or not as excess fluid may even jeopardize the condition. This article discusses physiological concepts of liberal versus restrictive fluid management followed by a discussion on the respective capabilities of various monitors to predict fluid responsiveness. The parameter difference in pulse pressure (dPP), derived from heart-lung interaction in mechanically ventilated patients is discussed in detail. The dPP cutoff value of 13% to predict fluid responsiveness is presented together with several assessment techniques of dPP. Finally, confounding variables on dPP measurements, such as ventilation parameters, pneumoperitoneum and use of norepinephrine are also mentioned.

Impact of working memory training on memory performance in old-old adults

Memory impairments constitute an increasing objective and subjective problem with advancing age. The aim of the present study was to investigate the impact of working memory training on memory performance. The authors trained a sample of 80-year-old adults twice weekly over a time period of 3 months. Participants were tested on 4 different memory measures before, immediately after, and 1 year after training completion. The authors found overall increased memory performance in the experimental group compared to an active control group immediately after training completion. This increase was especially pronounced in visual working memory performance and, to a smaller degree, also in visual episodic memory. No group differences were found 1 year after training completion. The results indicate that even in old?old adults, brain plasticity is strong enough to result in transfer effects, that is, performance increases in tasks that were not trained during the intervention.

Different response to eccentric and concentric training in older men and women

Sarcopenia is the age-related loss of muscle mass and strength and has been associated with an increased risk of falling and the development of metabolic diseases. Various training protocols, nutritional and hormonal interventions have been proposed to prevent sarcopenia. This study explores the potential of continuous eccentric exercise to retard age-related loss of muscle mass and function. Elderly men and women (80.6 +/- 3.5 years) were randomized to one of three training interventions demanding a training effort of two sessions weekly for 12 weeks: cognitive training (CT; n = 16), conventional resistance training (RET; n = 23) and eccentric ergometer training (EET; n = 23). Subjects were tested for functional parameters and body composition. Biopsies were collected from M. vastus lateralis before and after the intervention for the assessment of fiber size and composition. Maximal isometric leg extension strength (MEL: +8.4 +/- 1.7%) and eccentric muscle coordination (COORD: -43 +/- 4%) were significantly improved with EET but not with RET (MEL: +2.3 +/- 2.0%; COORD: -13 +/- 3%) and CT (MEL: -2.3 +/- 2.5%; COORD: -12 +/- 5%), respectively. We observed a loss of body fat (-5.0 +/- 1.1%) and thigh fat (-6.9 +/- 1.5%) in EET subjects only. Relative thigh lean mass increased with EET (+2.5 +/- 0.6%) and RET (+2.0 +/- 0.3%) and correlated negatively with type IIX/type II muscle fiber ratios. It was concluded that both RET and EET are beneficial for the elderly with regard to muscle functional and structural improvements but differ in their spectrum of effects. A training frequency of only two sessions per week seems to be the lower limit for a training stimulus to reveal measurable benefits.

Automatic algorithm for monitoring systolic pressure variation and difference in pulse pressure

BACKGROUND: Difference in pulse pressure (dPP) reliably predicts fluid responsiveness in patients. We have developed a respiratory variation (RV) monitoring device (RV monitor), which continuously records both airway pressure and arterial blood pressure (ABP). We compared the RV monitor measurements with manual dPP measurements. METHODS: ABP and airway pressure (PAW) from 24 patients were recorded. Data were fed to the RV monitor to calculate dPP and systolic pressure variation in two different ways: (a) considering both ABP and PAW (RV algorithm) and (b) ABP only (RV(slim) algorithm). Additionally, ABP and PAW were recorded intraoperatively in 10-min intervals for later calculation of dPP by manual assessment. Interobserver variability was determined. Manual dPP assessments were used for comparison with automated measurements. To estimate the importance of the PAW signal, RV(slim) measurements were compared with RV measurements. RESULTS: For the 24 patients, 174 measurements (6-10 per patient) were recorded. Six observers assessed dPP manually in the first 8 patients (10-min interval, 53 measurements); no interobserver variability occurred using a computer-assisted method. Bland-Altman analysis showed acceptable bias and limits of agreement of the 2 automated methods compared with the manual method (RV: -0.33% +/- 8.72% and RV(slim): -1.74% +/- 7.97%). The difference between RV measurements and RV(slim) measurements is small (bias -1.05%, limits of agreement 5.67%). CONCLUSIONS: Measurements of the automated device are comparable with measurements obtained by human observers, who use a computer-assisted method. The importance of the PAW signal is questionable.

Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes.

The survival of motor neurons (SMN) complex mediates the assembly of small nuclear ribonucleoproteins (snRNPs) involved in splicing and histone RNA processing. A crucial step in this process is the binding of Sm proteins onto the SMN protein. For Sm B/B', D1, and D3, efficient binding to SMN depends on symmetrical dimethyl arginine (sDMA) modifications of their RG-rich tails. This methylation is achieved by another entity, the PRMT5 complex. Its pICln subunit binds Sm proteins whereas the PRMT5 subunit catalyzes the methylation reaction. Here, we provide evidence that Lsm10 and Lsm11, which replace the Sm proteins D1 and D2 in the histone RNA processing U7 snRNPs, associate with pICln in vitro and in vivo without receiving sDMA modifications. This implies that the PRMT5 complex is involved in an early stage of U7 snRNP assembly and hence may have a second snRNP assembly function unrelated to sDMA modification. We also show that the binding of Lsm10 and Lsm11 to SMN is independent of any methylation activity. Furthermore, we present evidence for two separate binding sites in SMN for Sm/Lsm proteins. One recognizes Sm domains and the second one, the sDMA-modified RG-tails, which are present only in a subset of these proteins.

Ultrafast Relaxation Dynamics of Osmium-Polypyridine Complexes in Solution

We present steady-state absorption and emission spectroscopy and femtosecond broadband photoluminescence up-conversion spectroscopy studies of the electronic relaxation of Os(dmbp)3 (Os1) and Os(bpy)2(dpp) (Os2) in ethanol, where dmbp is 4,4′-dimethyl-2,2′-biypridine, bpy is 2,2′-biypridine, and dpp is 2,3-dipyridyl pyrazine. In both cases, the steady-state phosphorescence is due to the lowest 3MLCT state, whose quantum yield we estimate to be ≤5.0 × 10–3. For Os1, the steady-state phosphorescence lifetime is 25 ns. In both complexes, the photoluminescence excitation spectra map the absorption spectrum, pointing to an excitation wavelength-independent quantum yield. The ultrafast studies revealed a short-lived (≤100 fs) fluorescence, which stems from the lowest singlet metal-to-ligand-charge-transfer (1MLCT) state and decays by intersystem crossing to the manifold of 3MLCT states. In addition, Os1 exhibits a 50 ps lived emission from an intermediate triplet state at an energy 2000 cm–1 above that of the long-lived (25 ns) phosphorescence. In Os2, the 1MLCT–3MLCT intersystem crossing is faster than that in Os1, and no emission from triplet states is observed other than the lowest one. These observations are attributed to a higher density of states or a smaller energy spacing between them compared with Os1. They highlight the importance of the energetics on the rate of intersystem crossing.

Postural observación : los padres y los niños la percepción de su propia postura en la edad puberal. Encuesta en la escuela secundaria

El objetivo del presente estudio fue investigar los efectos de la percepción de competencia deportiva de desarrollar una investigación experimental en la escuela (Bunnell, WP., 1984) a fin de observar cómo pueden existir relaciones entre la auto-descripción de la postura, la postura vista por los padres y una observación postural objetiva

Construcción de información para la toma de decisiones : La política de Extensión de la Jornada Escolar en la provincia de Buenos Aires

Habitualmente el diseño y la gestión de políticas públicas se producen en escenarios complejos donde los aspectos técnicos y políticos se entrecruzan. Desde nuestro posicionamiento, resulta imprescindible considerar al planeamiento como una herramienta política que oriente la toma de decisiones en contextos de incertidumbre con sustento técnico. El presente trabajo tiene por finalidad describir la experiencia desarrollada desde la Dirección Provincial de Planeamiento (DPP) de la Dirección General de Cultura y Educación en relación con la construcción de herramientas de información que orienten la toma de decisiones en políticas educativas. Particularmente, se aborda la caracterización del dispositivo de trabajo elaborado para el monitoreo e implementación de la política de extensión de jornada escolar en el Nivel Primario. En primer lugar, se describen las características de esta política a nivel nacional, los antecedentes a nivel provincial y se analiza la situación de las instituciones que cuentan con algún formato de extensión del tiempo escolar. En segundo lugar, se presenta el dispositivo de abordaje elaborado por la DPP que incluye el análisis del cumplimiento de las metas de extensión de jornada, el costeo de su implementación y los criterios de selección de las instituciones educativas para extender su jornada escolar

Postural observación : los padres y los niños la percepción de su propia postura en la edad puberal. Encuesta en la escuela secundaria

El objetivo del presente estudio fue investigar los efectos de la percepción de competencia deportiva de desarrollar una investigación experimental en la escuela (Bunnell, WP., 1984) a fin de observar cómo pueden existir relaciones entre la auto-descripción de la postura, la postura vista por los padres y una observación postural objetiva

Construcción de información para la toma de decisiones : La política de Extensión de la Jornada Escolar en la provincia de Buenos Aires

Habitualmente el diseño y la gestión de políticas públicas se producen en escenarios complejos donde los aspectos técnicos y políticos se entrecruzan. Desde nuestro posicionamiento, resulta imprescindible considerar al planeamiento como una herramienta política que oriente la toma de decisiones en contextos de incertidumbre con sustento técnico. El presente trabajo tiene por finalidad describir la experiencia desarrollada desde la Dirección Provincial de Planeamiento (DPP) de la Dirección General de Cultura y Educación en relación con la construcción de herramientas de información que orienten la toma de decisiones en políticas educativas. Particularmente, se aborda la caracterización del dispositivo de trabajo elaborado para el monitoreo e implementación de la política de extensión de jornada escolar en el Nivel Primario. En primer lugar, se describen las características de esta política a nivel nacional, los antecedentes a nivel provincial y se analiza la situación de las instituciones que cuentan con algún formato de extensión del tiempo escolar. En segundo lugar, se presenta el dispositivo de abordaje elaborado por la DPP que incluye el análisis del cumplimiento de las metas de extensión de jornada, el costeo de su implementación y los criterios de selección de las instituciones educativas para extender su jornada escolar

Postural observación : los padres y los niños la percepción de su propia postura en la edad puberal. Encuesta en la escuela secundaria

El objetivo del presente estudio fue investigar los efectos de la percepción de competencia deportiva de desarrollar una investigación experimental en la escuela (Bunnell, WP., 1984) a fin de observar cómo pueden existir relaciones entre la auto-descripción de la postura, la postura vista por los padres y una observación postural objetiva

Construcción de información para la toma de decisiones : La política de Extensión de la Jornada Escolar en la provincia de Buenos Aires

Habitualmente el diseño y la gestión de políticas públicas se producen en escenarios complejos donde los aspectos técnicos y políticos se entrecruzan. Desde nuestro posicionamiento, resulta imprescindible considerar al planeamiento como una herramienta política que oriente la toma de decisiones en contextos de incertidumbre con sustento técnico. El presente trabajo tiene por finalidad describir la experiencia desarrollada desde la Dirección Provincial de Planeamiento (DPP) de la Dirección General de Cultura y Educación en relación con la construcción de herramientas de información que orienten la toma de decisiones en políticas educativas. Particularmente, se aborda la caracterización del dispositivo de trabajo elaborado para el monitoreo e implementación de la política de extensión de jornada escolar en el Nivel Primario. En primer lugar, se describen las características de esta política a nivel nacional, los antecedentes a nivel provincial y se analiza la situación de las instituciones que cuentan con algún formato de extensión del tiempo escolar. En segundo lugar, se presenta el dispositivo de abordaje elaborado por la DPP que incluye el análisis del cumplimiento de las metas de extensión de jornada, el costeo de su implementación y los criterios de selección de las instituciones educativas para extender su jornada escolar

Comparison of TEX86 and UK'37 temperature proxies in sinking particles in the Cariaco Basin

The Cariaco Basin, a silled, permanently anoxic basin on the continental shelf of Venezuela with a dynamic chemocline (-240-350 m), has been subject of > 20 years of oceanographic observation and sediment trap studies. We evaluated UK'37 and the TEX86 temperature proxies using sinking particles collected in shallow sediment trap samples at 275 m (Trap A) and 455 m (Trap B) (within and below the chemocline). The organic geochemical temperature proxies, UK'37. (based on coccolithophorid alkenone lipids) and TEX86 (based on archaeal glycerol dialkyl glycerol tetraether (GDGT) lipids), use observed relationships between the ratio of specific lipids and measured sea surface temperature to hindcast past sea surface temperatures. In this study, both UK'37 and TEX86 temperature proxies record seasonal temperature variations, including the cooling associated with upwelling events. UK'37-based temperatures are colder than measured sea surface temperatures, and better correlated temperature at the chlorophyll maximum. In sediment trap material collected below the chemocline (Trap B), UK'37 values are higher than those in Trap A. Warmer subchemocline UK'37 based temperatures may be related to autooxidation of sinking particles, either by small amounts of available oxygen or by alternate electron acceptors concentrated in the biologically dynamic chemocline (e.g. intermediate sulfur compounds). The absolute flux weighted TEX86 temperature values measured in sinking particles from Trap A match the measured SST well. The differences in the TEX86 values between Traps A and B are small and reflect less impact of degradation. Overall, the TEX86 temperatures in sinking particles in the Cariaco Basin reflect annual SST.