Analysis of human leukocyte antigens class II-DR in Brazilian children and adolescents with systemic lupus erythematosus

OBJECTIVE: To analyze the frequency of human leukocyte antigens class II-DR in children and adolescents with systemic lupus erythematosus. PATIENTS AND METHODS: Fifty-fiveBrazilian systemic lupus erythematosus children and adolescents and 308 healthy individuals were studied. Gender, race, and age of onset of systemic lupus erythematosus were recorded. The human leukocyte antigens typing of class II-DR was carried out by polymerase chain reaction amplification with sequence-specific primers (PCR-SSP). Data were analyzed statistically using the chi square test with Yates' correction, Fisher's exact test, and Bonferroni's correction. RESULTS: Human leukocyte antigen-DR 15 was the most frequently detected antigen in this group of children and adolescents, and it also occurred more frequently in the female group, in children with onset of systemic lupus erythematosus between 0 and 9 years and between 10 to 14 years, and in the Black race group, but these associations were not statistically significants. CONCLUSION: In this group of children and adolescents with a high degree of racial admixture, we could not verify a significant association between human leukocyte antigens class II-DR and systemic lupus erythematosus.

Dental and facial characteristics of patients with juvenile idiopathic arthritis

OBJECTIVE: It has been shown that the temporomandibular joint is frequently affected by juvenile idiopathic arthritis, and this degenerative disease, which may occur during facial growth, results in severe mandibular dysfunction. However, there are no studies that correlate oral health (tooth decay and gingival diseases) and temporomandibular joint dysfunction in patients with juvenile idiopathic arthritis. The aim of this study is to evaluate the oral and facial characteristics of the patients with juvenile idiopathic arthritis treated in a large teaching hospital. METHOD: Thirty-six patients with juvenile idiopathic arthritis (26 female and 10 male) underwent a systematic clinical evaluation of their dental, oral, and facial structures (DMFT index, plaque and gingival bleeding index, dental relationship, facial profile, and Helkimo's index). The control group was composed of 13 healthy children. RESULTS: The mean age of the patients with juvenile idiopathic arthritis was 10.8 years; convex facial profile was present in 12 juvenile idiopathic arthritis patients, and class II molar relation was present in 12 (P = .032). The indexes of plaque and gingival bleeding were significant in juvenile idiopathic arthritis patients with a higher number of superior limbs joints involved (P = .055). Anterior open bite (5) and temporomandibular joint noise (8) were present in the juvenile idiopathic arthritis group. Of the group in this sample, 94% (P = .017) had temporomandibular joint dysfunction, 80% had decreased mandibular opening (P = 0.0002), and mandibular mobility was severely impaired in 33% (P = .015). CONCLUSION: This study confirms that patients with juvenile idiopathic arthritis a) have a high incidence of mandibular dysfunction that can be attributed to the direct effect of the disease in the temporomandibular joint and b) have a higher incidence of gingival disease that can be considered a secondary effect of juvenile idiopathic arthritis on oral health.

Low coronary perfusion pressure is associated with endocardial fibrosis in a rat model of volume overload cardiac hypertrophy

Left ventricular hypertrophy following volume overload is regarded as an example of cardiac remodeling without increased fibrosis accumulation. However, infarction is associated with increased fibrosis within the noninfarcted, hypertrophied myocardium, particularly in the subendocardial regions. It is conceivable to suppose that, as also occurs postinfarction, low coronary driving pressure may also interfere with accumulation of myocardial fibrosis following aortocaval fistula. PURPOSE: To investigate the role of acute hemodynamic changes in subsequent deposition of cardiac fibrosis in response to aortocaval fistula. METHOD: Aortocaval fistula were created in 4 groups of Wistar rats that were followed over 4 and 8 weeks: aortocaval fistula 4 and aortocaval fistula 8 (10 rats each) and their respective controls (sham-operated controls - Sh), Sh4 and Sh8 (8 rats each). Hemodynamic measurements were performed 1 week after surgery. Hypertrophy and fibrosis were quantified by myocyte diameter and collagen volume fraction at the end of follow up. RESULT: Compared with Sh4 and Sh8, pulse pressure, left ventricular end-diastolic pressure, and +dP/dt were higher in aortocaval fistula 4 and aortocaval fistula 8, but -dP/dt was similar. Coronary driving pressure (mm Hg), used as an estimate of perfusion pressure, was lower in aortocaval fistula 8 (52.6 ± 4.1) than in Sh8 (100.8 ± 1.3), but comparable between aortocaval fistula 4 (50.0 ± 8.9) and Sh4 (84.8 ± 2.3). Myocyte diameter was greater in aortocaval fistula 8, whereas interstitial and subendocardial fibrosis were greater in aortocaval fistula 4 and aortocaval fistula 8. Coronary driving pressure correlated inversely and independently with subendocardial fibrosis (r² = .86, P <.001), whereas left ventricular systolic pressure (r² = 0.73, P = .004) and end-diastolic pressure (r² = 0.55, P = 012) correlated positively and independently with interstitial fibrosis. CONCLUSION: Coronary driving pressure falls and ventricular pressures increase early after aortocaval fistula and are associated with subsequent myocardial fibrosis deposition.

Ethnoveterinary knowledge of the inhabitants of Marajó Island, Eastern Amazonia, Brazil

There have been ethnoveterinary reports from around the world investigating plant usage in therapeutic protocols; however, there is no information regarding the ethnoveterinary practices in Brazilian Amazonia. The objective of this work was to register and document the ethnoveterinary knowledge of the inhabitants of the Island of Marajó, eastern Amazonia, Brazil. In the study, interviews were conducted with 50 individuals, with the application of semi-structured questionnaires that were quantitatively analyzed using descriptive statistic methods of frequency distribution. Use-value was calculated to determine the most important species. Samples of plants that were reported to have medicinal value were collected and identified by botanical classification. Fifty plants, distributed among 48 genera and 34 families, were indicated for 21 different medicinal uses. The family Asteraceae had the largest number of reported species; Carapa guianensis Aubl., Copaifera martii Hayne, Crescentia cujete L., Caesalpinia ferrea Mart., Chenopodium ambrosioides L., Jatropha curcas L. and Momordica charantia L. were species with highest use- value. The plant parts that were more commonly utilized for the preparation of ethnoveterinary medicines were the leaves (56%), bark (18%), roots (14%), seeds (14%) and fruit (8%). With regard to usage, tea was reported as a usage method by 56% of the informants; most preparations (90.9%) utilized only a single plant. In addition to medicinal plants, informants reported using products of animal and mineral origin. The present study contributed to the construction of an inventory of Marajó Island's ethnoveterinary plants, which might be the basis for future scientific validation studies.

Apical aneurysm and left ventricular hypertrophy

A 59-year-old woman presented with an embolic transient ischemic attack and a history of controlled hypertension for 16 years. Both echocardiogram and MRI showed severe biventricular hypertrophy and an apical aneurysm with a thrombus. The occurrence of an apical aneurysm in the presence of cardiac hypertrophy is a rare finding and has been described in patients with hypertrophic cardiomyopathy. However, it has not been reported in patients with systemic arterial hypertension. In this patient the lack of a relationship between the severity of the hypertrophy and the levels of blood pressure, together with the presence of histologic disorganization of myocardial cardiac muscle cells by endomyocardial biopsy suggested the diagnosis of hypertrophic cardiomyopathy.

Chagas' heart disease: evolutive evaluation of electrocardiographic and echocardiographic parameters in patients with the indeterminate form

OBJECTIVE: To identify and associate potential electrocardiographic and echocardiographic changes in patients with the indeterminate form of Chagas' disease during long-term follow-up. METHODS: One hundred sixty patients underwent standard electrocardiography and two-dimensional guided M-mode echocardiography for left ventricular ejection fraction determination. Patients were followed up for 98.6±30.4 months, undergoing repeat electrocardiographic studies at 6-month intervals and echocardiographic studies at 12-month intervals. RESULTS: Based on the electrocardiographic findings, the patients were divided into group I, 125 patients (78.6%) with normal electrocardiograms throughout follow-up, and group II, 34 patients (21.3%) who developed electrocardiographic changes. Group II was further divided into group IIA (9 patients, 5.6%) with permanent electrocardiographic changes, group IIB (14 patients, 8.8%) with transitory electrocardiographic changes, and group IIC (11 patients, 6.9%) with changes appearing only on the final electrocardiogram. Left ventricular ejection fractions remained normal in the entire population studied and did not differ among groups. CONCLUSION: The indeterminate form of Chagas' disease clearly represents a benign condition with a favorable long-term prognosis. Although some patients develop electrocardiographic changes, left ventricular systolic function is well preserved.

Subendocardial fibrosis in remote myocardium results from reduction of coronary driving pressure during acute infarction in rats

OBJECTIVE: To investigate the role of hemodynamic changes occurring during acute MI in subsequent fibrosis deposition within non-MI. METHODS: By using the rat model of MI, 3 groups of 7 rats each [sham, SMI (MI <30%), and LMI (MI >30%)] were compared. Systemic and left ventricular (LV) hemodynamics were recorded 10 minutes before and after coronary artery ligature. Collagen volume fraction (CVF) was calculated in picrosirius red-stained heart tissue sections 4 weeks later. RESULTS: Before surgery, all hemodynamic variables were comparable among groups. After surgery, LV end-diastolic pressure increased and coronary driving pressure decreased significantly in the LMI compared with the sham group. LV dP/dt max and dP/dt min of both the SMI and LMI groups were statistically different from those of the sham group. CVF within non-MI interventricular septum and right ventricle did not differ between each MI group and the sham group. Otherwise, subendocardial (SE) CVF was statistically greater in the LMI group. SE CVF correlated negatively with post-MI systemic blood pressure and coronary driving pressure, and positively with post-MI LV dP/dt min. Stepwise regression analysis identified post-MI coronary driving pressure as an independent predictor of SE CVF. CONCLUSION: LV remodeling in rats with MI is characterized by predominant SE collagen deposition in non-MI and results from a reduction in myocardial perfusion pressure occurring early on in the setting of MI.

Epidemiologia da insuficiência cardíaca descompensada em Niterói: Projeto EPICA - Niterói

OBJETIVO: Comparar entre os hospitais públicos e privados de Niterói o perfil epidemiológico, sócio-econômico, aspectos clínicos, etiologia, tempo de internação e a mortalidade dos pacientes internados por insuficiência cardíaca descompensada. MÉTODOS: Estudo transversal prospectivo e multicêntrico (julho a setembro/ 2001), incluindo todos os pacientes internados com diagnóstico primário de insuficiência cardíaca, maiores de 18 anos, e com pontuação 8 ou superior pelos critérios de Boston. RESULTADOS: Incluídos 203 pacientes: 98 públicos, 50% eram homens, média de idade de 61,1± 11,3 anos, 65% negros, 57% recebiam até 1 salário mínimo, 56% eram analfabetos, 66% tinham cardiopatia isquêmica; tempo médio de internação de 12,6 dias e a taxa de mortalidade ajustada para a idade de 5,23; 105 privados, 49% eram homens, média de idade de 72 ± 12,7 anos, 20% negros, 58% recebiam mais de 6 salários mínimos, 11% eram analfabetos, 62% eram isquêmicos; o tempo médio de internação hospitalar de 8 dias e a taxa de mortalidade ajustada para idade de 2,94. A distribuição das co-morbidades e fatores de risco similar entre os pacientes, à exceção do tabagismo, mais freqüente no serviço público. CONCLUSÃO: Além de assimetrias sócio-econômicas, o tempo de internação hospitalar e a taxa mortalidade ajustada para idade foram maiores no serviço público de saúde.

Anatomia, dos nectános de algumas espécies da flora apícola

This paper deals with anatomical descriptions of some types of nectaries in 27 species of honey plants of Piracicaba, S. P. The material studied was divides in two groups: a) Extra-floral nectaries; b) Floral nectaries. Euphorbia pulcherrima, Willd; showed to belonging to the first group: its nectaries tissue consist of an epidermal layer of cell without stomata and with true gland, with subepidermal cells diferentiated by the thickness of the wall. Among the plants with floral nectaries, the following types has been listed, according the location of the nectary in the flower: 1 - with true glands: a) in sepals, Hibiscus rosa sinensis, L.; Dombeya Wallichii, Bth. e Hk; b) in the stamens tube, Antigonum leptopus, Hook e Arn.; 2 - on the receptacle with nectariferous tissue in the epidermal cell with: a) thickness wall with stomata, Prunus persical, L.; b) thin wall without stomata, Crotalaria paulinia, Shranck; Caesal-pinia sepiaria, Roxb; Aberia caffra; 3 - with a disc located in the receptacle with: epidermal: a) with stomata, Coffea arábica, L. var. semper florens; Citrus aurantifolia, Swing; Cinchona sp.; Pryrostegia ignea, Presl.; b) without stomata and with thin wall, Leojurus sibiricus, L.; Bactocydia unguis, Mart., Ipomoea purpurea, L.; Greviüea Thelemanniana, Hueg.; Dolichos lablab, L.; Vernonia polyanthes, Less., Montanoa bipinatifida, C. Koch., Eruca sativa, L. Brassica Juncea, Co; Eucalyptus tereticomis, Smith.; Eucalyptus rostrata, Schleche; Salvia splendens, Selow.; 4 - in the basal tissues of the ovary, Budleia brasiliensis, Jacq F.; Petrea subserrata, Cham.; 5 - in the base of stamens, Per sea americana, Mill. On the anatomical point of view, most of the types of nectary studied has external nectariferous tissues, located on the epidermal cells with thin periclinal wall and without stomata. The sub-epidermal layer were rich in sugar. Short correlation was found between the structure of the nectary and the amount of nectar secretion. So, in the nectary with true glands, in those with thin wall and without stomata on epidermal cells and in those with stomata, the secretion was higher than in the other types listed.

Sôbre uma fórmula para o cálculo da dose mais econômica de adubo

The authors discuss a formula for the determination of the most profitable level of fertilization (x*). This formula, presented by CAREY and ROBINSON (1953), can be written as: x*= (1/c) log cx u L10 + (1/c) log wu _______ ___ 1-10 x u t being c the growth factor in Mitscherlich's equation, x u a standard dressing of the nutrient, L 10 the Naeperian logarithm of 10, u the response to the standard dressing, w the unit price of the crop product, and i the unit price of the nutrient. This formula is a modification of one of the formulas of PIMENTEL GOMES (1953). One of its advantages is that is does not depend on A, the theoretical maximum harvest, which is not directly given by experimental data. But another advantage, proved in this. paper, is that the first term on the right hand side K= 1(/c) log cx u L 10 ____________ 1 - 10-cx u is practically independent of c, and approximately equivalent to (1/2) x u. So, we have approximately x* = (1/2) x u + (1/c) log wu . ____ x u t With experimental data we compute z = wu ____ x u t then using tables 1, 2 and 3, we may obtain Y - (1/c) log z and finally x* = (1/2) x u + Y. This is an easy way to determine the most profitable level of fertilization when experimental data on the response u to a dressing x u are available. Tables for the calculation of Y are included, for nitrogen, phosphorus, potash, and manure.

Novas variedades de Cana-de-Açúcar

The authors carried out joint analyses of data referring to six experiments with varieties of sugar cane, carried out by SEGALLA and ALVAREZ in six locations in the State of S. Paulo, Brasil. The analyses showed that for cane or sugar yield, either for plant-cane or for plant-cane together with the first two ratoons, the best five varieties were CB 40-69, CB 41-76, CB 40-13, CB 40-19 and Co 419. The yield of sugar cane/for all varieties studied is given below, in metric tons produced in plant cane and the first two ratoons. Varieties Yield of sugar cane (tons/hectare) CB 40-69 205.2 CB 41-76 204.5 CB 40-13 199.4 CB 40-19 192.4 Co 419 192.1 CB 38-30 182.1 CB 41-70 181.5 Co 413 177.5 CB 38-22 174.4 CB 36-14 172.8 Co 290 166.6 CB 41-35 147.9 The least significant difference by Tukey's test, at the 5% level of probability, is A = 28.3 metric tons/hectare.

Influência do desponte sôbre a composição do côlmo e do caldo da cana-de-açucar, I. Var. Co 421

I. This paper deals with an experiment carried out to evaluate the effect of the sugar cane upper end on the composition of the sugar cane harvest as a raw material for the sugar industry. The variety studied was Co 421. The authors intend to study other varieties in the future. The data were collected from plant cane, at intervals of two weeks, always from the same field, from a small central area of 3.000 square meters approximately. Sixty (60) stalks were cut in each occasion, randomly chosen from the whole area. They were afterwards separated into three groups of 20 stalks, one for each of the treatments, namely: a) Complete stalks, with no leaves or sheaths. b) Stalks harvested by the technique of REYNOSO, that is, as usually done in practice. c) Stalks with the tops completely cut out, that is, cut by technique of REYNOSO and then with 3 other top internodes eliminated. The treatments caused significant differences on the following technological characteristics: a) Weight b) Cane pol c) Available sucrose (pol) per cent cane d) Cane juice pol e) Saline coefficient of juice. II. Except for weight, all changes were favorable to treatment c, even if with differences relatively slight, in percentage. IIII. Treatment differences for cane fiber, brix, reducing sugars, juice ashes, coefficient of purity and glucose coefficient were not significant. IV. Time of harvest was an important factor affecting the composition of the cane and of the juice. V. On the average the available sucrose of cane for treatments, with an standard error of 0.13%, was: Treatment c 13.05% Treatment b 12.65% Treatment a 12.53% This shows that there is no sound basis for the heavy fines applied by some sugar mills to planters who do not cut low enough the tops of the cane stalks.

A amostragem da cana-de-açúcar para determinações tecnológicas

The authors carried out 3 experiments on the sampling of sugar cane for technological determinations, one with each of the varieties Co 419, CB 40-69 and CB 41-58, in Piracicaba, State of São Paulo, Brasil. The main intent of the project was to compare 2 methods of sampling, namely: 1) Method A, where the sample is a hill (CATANI et al, 1959) or, more generally, 20 stalks all together in a randomly selected point of the furrow; 2) Method B, where 20 stalks are taken, from 20 points evenly spread but on the whole plot. Coefficients of variation for 20 stalk samples Variety Characteristic 20 stalks per hill 1 stalk per hill Brix 4.8% 1.9% Pol 6.4% 2.5% CB 40-69 Coefficient of purity 2.1% 0.83% Available sucrose 7.3% 2.7% Weight 6.6% 6.9% Brix 5.3% 1.8% Pol 7.6% 2.6% Co 419 Coefficient of purity 2.9% 1.0% Available sucrose 8.6% 3.0% Weight 21.2% 6.5% Brix 2.8% 1.4% Pol 4.1% 1.9% CB 41-58 Coefficient of purity 1.8% 0.8% Available sucrose 5.0% 2.2% Weight 10.9% 6.2% For the 3 varieties studied and for the data on Brix, pol, coefficient of purity, available sucrose and weight, analyses of variance were carried out. Further computations led to the following coefficients of variation. For available sucrose, which is probably the most important characteristic studied, the average coefficient of variation for the 3 varieties was 2.7%, for the case of method B, that is, 20 stalk samples, one stalk per hill. Assuming this coefficient of variation, in a trial with 5 treatments and 6 replications, in randomised blocks, the least significant difference among treatment means, at the 5% level, would be 4.7% of available sucrose by Tukey's test, and 3.3% by the t test. For the case of method A the average coefficient of variation is 7.0% and, in similar conditions, the least significant difference would be 15.1% by Tukey's test, and 12.1% by the t test. Since differences of available sucrose among treatments in experiments with fertilizers seldom are higher than 3 or 4% of the mean (PIMENTEL GOMES & CARDOSO, 1958), method B with a 20 stalk sample per plot gives more or less the minimum amount of cane to be sampled for technological determinations. In experiments with varieties, however, where differences may be assumed to be higher, a sample of 10 to 20 stalks one per hill, can be enough.

Influência do desponte sôbre a composição do colmo e do caldo da cana-de-açúcar: III. Var. CB 41-76

I. This paper deals with an experiment carried out to evaluate the effect of sugar cane upper end on the composition of the stalks and juice of sugar cane harvest as a raw material for the sugar industry. The variety studied was CB 41-76. The data were collected from plant cane at intervals of a two weeks, always from the same field, from a small central area of 3.000 square meters approximately, 60 stalks were cut in each occasion, randomly chosen from the whole area. They were afterwards separated into three groups of 20 stalks one for each of the treatments, namely: a) Complete stalk, with no leaves or sheaths. b) Stalks harvested by the technique of REYNOSO, that is, as usually done in practice. c) Stalks with the tops completely cut out, that is, cut by the techinique of REYNOSO and then with 3 other top internodes eliminated. The treatments caused significant differences on the weight of cane and coefficient of purity of juice, but the percentual differences between the average treatments a and c is 13% and 2%, respectively. II. Treatment differences for cane pol, cane fibre, brix, juice pol, reducing sugars, juice ashes, glucose coefficient, saline coefficient and available sucrose (pol) per cent were not significant. III. Time of harvest was an important factor affecting the composition of the cane and the juice. This paper shows that there is no sound basis for the heavy fines applied some sugar mills to planters who do not cut low enough the tops of the cane stalks.