Intersubunit contacts made by tryptophan 120 with biotin are essential for both strong biotin binding and biotin-induced tighter subunit association of streptavidin.

In natural streptavidin, tryptophan 120 of each subunit makes contacts with the biotin bound by an adjacent subunit through the dimer-dimer interface. To understand quantitatively the role of tryptophan 120 and its intersubunit communication in the properties of streptavidin, a streptavidin mutant in which tryptophan 120 is converted to phenylalanine was produced and characterized. The streptavidin mutant forms a tetrameric molecule and binds one biotin per subunit, as does natural streptavidin, indicating that the mutation of tryptophan 120 to phenylalanine has no significant effect on the basic properties of streptavidin. However, its biotin-binding affinity was reduced substantially, to approximately 10(8) M-1, indicating that the contact made by tryptophan 120 to biotin has a considerable contribution to the extremely tight biotin binding by streptavidin. The mutant retained bound biotin over a wide pH range or with the addition of urea up to 6 M at neutral pH. However, bound biotin was efficiently released by the addition of excess free biotin due, presumably, to exchange reactions. Electrophoretic analysis revealed that the intersubunit contact made by tryptophan 120 to biotin through the dimer-dimer interface is the major interaction responsible for the biotin-induced, tighter subunit association of streptavidin. In addition, the mutant has weaker subunit association than natural streptavidin even in the absence of biotin, indicating that tryptophan 120 also contributes to the subunit association of tetramers in the absence of biotin.

A Poesia Herói-Cómica e a Memória de Bagatelas: A Celebração Épica de Trivialidades n’O Hissope de Cruz e Silva

Os poemas homéricos integram no tecido narrativo evidências do processo oral de transmissão poética a que as suas origens estão associadas. Seria, pois, a voz hábil e poderosa do aedo o meio através do qual os heróis do passado alcançavam renome, passando à póstuma memória como protagonistas das mais notáveis façanhas. Contudo, enquanto a epopeia celebriza em tom solene e grave a memória de um passado glorioso, contrariamente a poesia herói-cómica, que se caracteriza pelo contraste entre a expressão elevada e o tema vulgar, torna objecto de canto incidentes triviais. Neste capítulo, reflectirei sobre a subversão do valor encomiástico da poesia épica antiga n’ O Hissope de Cruz e Silva, poema herói-cómico português, que celebra, num estilo alto e sublimado, próprio da epopeia, uma das mais frívolas questiúnculas ocorridas na Sé de Elvas, na segunda metade de setecentos, entre o Deão José Lara e o Bispo D. Lourenço de Lancastro. Demonstrarei que o poema em causa glorifica, de forma paródica e satírica, acções grosseiras e insignificantes, inserindo a realidade social, não num passado heróico, mas num presente vicioso e ordinário. Assiste-se, assim, neste texto, a uma imitação dos recursos épicos não para imortalizar feitos gloriosos, mas para preservar a memória de bagatelas. Significativo é o episódio (cuja análise dou especial atenção) em que Vidigal entretém o banquete em casa do deão, no canto VII de O Hissope. O padre cantor afirma categórico que deixará em silêncio os grandes sucessos “em mais remotos tempos celebrados”, optando por glorificar a memoranda Elvas com o relato musical de três incidentes triviais nela ocorridos.

First Annual IMACS Report: A global International Society for Heart and Lung Transplantation Registry for Mechanical Circulatory Support

The first annual report of the International Society for Heart and Lung Transplantation (ISHLT) Mechanically Assisted Circulatory Support (IMACS) registry provides global data on 5,942 patients from 31 countries. This initial report focuses on patient demographics, survival, device types, adverse events, competing outcomes, and a risk factor analysis.

University of Michigan Field Hockey Team, 1986

Front Row: trainer Kim Godek, manager Cheryl Mick, Sara Clark, Sharon Cantor, Diane Pentaleri, Lisa Murray, Joan Taylor, Andrea Kuebbeler, Margaret Kundtz, Ilene Meadows, assistant coach Andrea Wickerham;

Back Row: head trainer Sue Peel, Gillian Pieper, Jane Nixon, Debbie Devine, Judy Burinskas, Patti Farley, Trisha Mondul, Katrina Warner, Tracy Gaskins, Dorie McCubbrey, Robin Ives, Maryann Bell, Head Coach Karen Collins.

(Not pictured: Joanne Green)

University of Michigan Field Hockey Team, 1987

Back Row: head coach Karen Collins, Joanne Green, Josee Charvet, Debbie Devine, Carolyn Fancher, Katrina Warner, Judy Burinskas, Gillian Pieper, ?

Middle Row: Patricia Maran, Patti Farley, Ilene Meadows, Trisha Mondul, Diane Pentaleri, Kay King,

Front Row: assistant coach Patti Smith, Sharon Cantor, Robin Ives, Sara Clark, Andrea Kuebbeler, ?

Wide scope, questionable quality : three reports from the study on school violence and prevention : executive summary /

"Doc #2002-21."

Marcus Marulus Spalatensis De institutione beneuiuendi per exempla sanctorum.

Edit16,

Venerabilis Petri, Cantoris Ecclesiae B. Mariae Parisiensis, ... Verbum abbreviatum opus morale ab annis fere quingentis conscriptum, omnibus theologis, pastoribus, confesariis, concionatoribus, Iuris-consultis, & cuiuscumque conditionis hominibus utilissimum /

Mode of access: Internet.

Mathematische beiträge zum kulturleben der völker,

Mode of access: Internet.

Vorlesungen über Geschichte der Mathematik,

A 3d edition of v. 1 appeared in 1907.

Contributions to the founding of the theory of transfinite numbers.

Mode of access: Internet.

The Artistic tenor. [music] : a collection of standard ballads and arias /

Words in English or English and original language.

An evaluation of the trainer-tester and punchboard tutor as electronics trouble-shooting training aids,

Mode of access: Internet.

A history of the Bureau of the Public Debt : 1940-1990 with historical highlights from 1789-1939 /

S/N 048-011-00023-3

University of Michigan Field Hockey Team, 1989

Back Row: Jennifer Stevens, Lynn Miller, Laura Beer, Katie Thomas, Hilary Hughes, Sandi Marotti, Patricia Maran, Mary Beth Bird, head coach Patti Smith

Middle Row: Katie Vignevic, Keely Libby, Josee Charvet, Kristin Shaiper, Mary Peters, Katie Allison, Katherine Epler

Front Row: Margaret Kuntz, Ilene Meadows, Joanne Green, Sharon Cantor, Judy Burinskas, Patti Farley, student trainer Jim Peters.