1H HR-MAS NMR Based Metabolic Profiling of Cells in Response to Treatment with a Hexacationic Ruthenium Metallaprism as Potential Anticancer Drug.

1H high resolution magic angle spinning (HR-MAS) NMR spectroscopy was applied in combination with multivariate statistical analyses to study the metabolic response of whole cells to the treatment with a hexacationic ruthenium metallaprism [1]6+ as potential anticancer drug. Human ovarian cancer cells (A2780), the corresponding cisplatin resistant cells (A2780cisR), and human embryonic kidney cells (HEK-293) were each incubated for 24 h and 72 h with [1]6+ and compared to untreated cells. Different responses were obtained depending on the cell type and incubation time. Most pronounced changes were found for lipids, choline containing compounds, glutamate and glutathione, nucleotide sugars, lactate, and some amino acids. Possible contributions of these metabolites to physiologic processes are discussed. The time-dependent metabolic response patterns suggest that A2780 cells on one hand and HEK-293 cells and A2780cisR cells on the other hand may follow different cell death pathways and exist in different temporal stages thereof.

Visibility of lipid resonances in HR-MAS spectra of brain biopsies subject to spinning rate variation.

Lipid resonances from mobile lipids can be observed by (1)H NMR spectroscopy in multiple tissues and have also been associated with malignancy. In order to use lipid resonances as a marker for disease, a reference standard from a healthy tissue has to be established taking the influence of variable factors like the spinning rate into account. The purpose of our study was to investigate the effect of spinning rate variation on the HR-MAS pattern of lipid resonances in non-neoplastic brain biopsies from different regions and visualize polar and non-polar lipids by fluorescence microscopy using Nile Red staining. (1)H HR-MAS NMR spectroscopy demonstrated higher lipid peak intensities in normal sheep brain pure white matter biopsies compared to mixed white and gray matter biopsies and pure gray matter biopsies. High spinning rates increased the visibility particularly of the methyl resonances at 1.3 and the methylene resonance at 0.89ppm in white matter biopsies stronger compared to thalamus and brainstem biopsies, and gray matter biopsies. The absence of lipid droplets and presence of a large number of myelin sheaths observed in white matter by Nile Red fluorescence microscopy suggest that the observed lipid resonances originate from the macromolecular pool of lipid protons of the myelin sheath's plasma membranes. When using lipid contents as a marker for disease, the variable behavior of lipid resonances in different neuroanatomical regions of the brain and at variable spinning rates should be considered. The findings may open up interesting possibilities for investigating lipids in myelin sheaths.

Hr. Minister Eichmann wird gebohmhammelt, weil er den Weg zu de Freiheit verram̄elt: Eene reh-speck-volle Interpellation: Nämlich ick bitte Ihnen um Entschuldigung, wenn ick störe ...

Welsch (Projektbearbeiter): Kritische Auseinandersetzung mit dem Inhalt eines Rundschreibens des Innenministers von Eichmann vom 5. Oktober 1848, in dem vom Mißbrauch des Rechts auf Meinungs- und Versammlungsfreiheit die Rede ist und die zuständigen Beamten angewiesen werden, in solchen Fällen 'mit Nachdruck' einzuschreiten: "Ne, jutstet Eichmänneken, so lassen wir nich mehr mit uns reden. Det is 'ne vormärzliche Sprache, die Sie in Ihr Rundschreiben führen ..."

Aligning Library Strategy and Structure With the Campus Academic Plan: A Case Study

Colleges and universities’ missions are typically comprised of educating students, training professionals, engaging in scholarship and research, promoting creative activity, improving healthcare, and providing public service. Academic libraries exist to support these core functions, yet most academic libraries are organized based on library functions rather than the primary missions of their college or university. This paper describes one academic library’s attempt to align library strategy and structure with its university’s academic plan.

Cavallierischer Zwey-Kampf : vollzogen[n] den 7 Januarij Ao. 1678 - 28. Xbr. Ao. 1677 ; Hr. Johann Andreas Graf Corbelli Obristwachtmeister, Hr. Carol Fridrich Ferdinand V. Reich, Baron, Obristwachtmeister

G. I. Schneider fc.

Hr. B. Ankermann spricht über: einige Fetische aus Togo

B. Ankermann

Richard Wagner-Cyklus, Tannhäuser : Er kehrt nicht zurück! - Allmächt'ge Jungfrau, hör' mein Flehen! Zu dir, Gepris'ne, rufe ich! Lass mich im Staub vor dir vergehen.

Signatur des Originals: S 36/F10485

Beethovens Hör-Instrumente in den Jahren 1812-1814 von dem Mechaniker Maelzel in Wien für den Meister angefertigt : Verein Beethoven-Haus in Bonn

Signatur des Originals: S 36/F10603