Prevalência de papiloma vírus (HPV) perianal assintomático em pacientes portadores de HPV genital tratados no Hospital das Clínicas da Faculdade de Medicina de Botucatu

Introduction: Human Papillomavirus (HPV) infection is one of the Sexually Transmitted Diseases (STD) of greatest incidence and prevalence worldwide, and it is presently considered to be a pre-neoplastic lesion. Human Papillomavirus infection has totaled 23.4% of the sexually transmitted diseases reported to the Department of Health, and it is currently the most common in our country. Many patients are asymptomatic carriers. Methods: Twenty patients who had been previously treated for HPV due to genital lesions were referred for the conduction of anuscopy and colposcopy of the perianal region. Results: Males (80%) prevailed over females (20%). Of the total number of patients, only 2 showed lesions as examined by anuscopy (10%). However, 3 other patients showed lesions by means of colposcopy, thus increasing to 5 (25%) the total number of asymptomatic patients who presented perianal lesions. Of the total number of patients with lesions, 4 were males and 1 was a female. Conclusion: The presence of perianal lesions was observed in 10% of the patients with genital lesions by means of simple anal inspection. This figure increased to 25% when anal colposcopy was associated, thus showing the importance of conducting such examination on all patients with increased risk factors for HPV infection in the anal region.

Qualidade química da água residual da criação de peixes para cultivo de alface em hidroponia

Aiming to evaluate the association of hydroponic lettuce cultivation with residues from a fish intensive breeding system, a project was carried out in the Aquaculture Center in the FCAV-UNESP at Jaboticabal, SP, Brazil. A closed system was designed in order to allow the water to circulate through the fish tanks, a clarifier tank for removal of residues, a reservoir for biological conversion of ammonia into nitrate, and the hydroponic system. After this process, water returned back to the fish tanks. Three varieties of lettuce, constituting the treatments with four repetitions were evaluated. The results of the chemical analysis of the residual water from the fish tanks indicated the presence of the majority of the mineral nutrients necessary for vegetable development. Their concentration was close to that found in nutrient solutions, used for lettuce hydroponic cultivation, except for potassium and magnesium. The low concentration of magnesium in the water did not prevent lettuce development, although the plants presented visual symptoms of deficiency of this nutrient. Differences were not found between the varieties produced, regarding productivity and the mean weight of fresh plants.

Potenciais de água no solo na produção de alface

The scope of this experiment is to study the influence of soil water potential on lettuce productivity, particularly in relation to deficit and excess of water. Four lettuce cultivars (Americana, Roxa, Crespa and Mimosa), four minimum soil water potential (-0,001, -0,005, -0,012 and 0,022 MPa) and three replicates in experimental randomized design. The results allowed concluding that the -0,012 MPa has the tendency to produce the highest green mass among her soil water potential applied. The Mimosa showed the tendency to produce the highest evapotranspiration among the cultivars. The cultivars Americana e -0,05 MPa was the best combination (148,33g) the worst was the Roxa and -0,022 MPa minimum soil water potential.

Período total de prevenção a interferência das plantas daninhas na cultura da alface cultivar Solaris

This research work aimed to determine the total period of interference of weeds prevention of lettuce cultivar Solaris. The experiment was conducted in Universidade Estadual de Londrina, in the period of autumn/ winter of 2008. The lettuce crop remained free from weed interference from emergence up to 0, 10, 20, 30, 40, 50 e 60 days. The experiment was arranged in a randomized block design with four replications. The weed community was composed by Bidens pilosa, Amaranthus hybridus, Oxalis corniculata, Euphorbia heterophylla, Spinacia oleracea, Commelina benghalensis e Lepidium virginicum. It was determined dry and fresh matter of weeds, and number of lettuce leaves. Assuming a tolerance of 5% for crop production decrease, it was verified that the total period of interference prevention (TPIP) occurred up to 21 days after transplanting. Weed interference throughout the crop cycle reduced 25% of its yield.

Análise espacial de nutrientes e produção de alface cultivada em ambiente protegido

The objective of this work was to study spatial variability of some chemical soil attributes and lettuce production (total shoot fresh matter mass - MF; commercial shoot fresh matter mass - MFC; commercial shoot dry matter mass - MCS; and head commercial diameter - DCC) offering subsidies to the protected environment mapping in nutrients management areas in lettuce culture aiming for a higher productivity with application of fertilizers. The experiment was conducted in a protective environment (greenhouse) with lettuce irrigated by drip irrigation and sampling grid with 152 points. The special dependence analysis, determined by the variogram, was obtained with the aid of the GS+ Program. Considering the need for crop nutrients through the map obtained for element P (phosphorus) it was possible to establish two distinct areas for the application of this element in plantation fertilization. Through the lettuce yield maps obtained with MFC and DCC attributes was difficult to establish distinct areas for its management with data observed in only one crop cycle. Krigagem has proved useful for mapping the attributes studied.

Alface americana fertirrigada com diferentes doses de ácido fosfórico e hidróxido de potássio

Fertirrigation with phosphoric acid has been considered as an option to avoid drips from clogging. In some cases, this acid cause soil acidification and it can be substituted bypotassium hydroxide. This study had the objective of evaluating the effects of different phosphoric acid and potassium hydroxide doses, applied via fertirrigation, on soil fertility, plant nutrition and lettuce yield, compared to the conventional fertilization system. The experiment was carried out in greenhouse, in the College of Agricultural Sciences, FCA/UNESP, Botucatu-SP-Brazil. The experimental design was the completely randomized block, with 6 treatments and 4 replications. Treatments consisted of the combinations of doses (25% and 50% of the recommended complete dose for the lettuce) and application ofcomplete dose (100%) of phosphorus (P) and potassium (K) applied via fertirrigation, and a control (conventional fertilization system). The results showed that P and K availability in soil and nutrient concentration in plants were affected by treatments. Low phosphorus and potassium doses, applied via fertirrigation, were not efficient for the appropriate development of lettuce plants. The results showed that agronomic characteristics and commercial lettuce were affected by doses and application systems, especially the treatment with application of P and K full dose fertirrigation.

Variabilidade espacial na produção da alface em ambiente protegido

Este artigo mostra a dinâmica da água após ser aplicada sobre a superfície de um solo cultivado com alface, num sistema fechado de um ambiente protegido. A aplicação de água se deu por meio de um sistema de irrigação por gotejamento, com o CUD ( Coeficiente de Uniformidade de Distribuição ) igual a 97,06 %, o que garantiu uma ótima uniformidade de distribuição, desconsiderando como possíveis causas das variações de umidade do solo, a aplicação de lâminas variadas. Fazendo uso de técnicas de geoestatística, interpolando sessenta pontos amostrais de evaporação, umidade do solo e produtividade da alface, com as mesmas sessenta coordenadas para os três atributos, verificou-se que a umidade do solo, mesmo momentos após a irrigação, não permanece uniforme ao longo do sistema de irrigação, constatando-se que a evaporação local exerce influência de forma predominante sobre a dinâmica da água na superfície do solo. Concluiu-se que as regiões no interior da estufa de maiores evaporações, foram as de maiores variações de umidade do solo e na produção de massa verde da alface.

Sorofrequência de infecção pelo vírus da imunodeficiência felina e vírus da leucemia felina em gatos do município de araçatuba, são paulo

Feline immunodeficiency virus and feline leukemia represent important infectious diseases caused by retroviruses. This study aimed to investigate the prevalence of infection by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in cats from the municipality of Araçatuba, São Paulo. Blood samples from 302 cats were collected and tested for the presence of antibodies against feline immunodeficiency virus and antigen of feline leukemia virus by ELISA ® Snap- Combo FIV-FeLV (IDEXX Laboratories). The frequency of FIV positivity was 5.63% (17/302) and of FeLV was 0.33% (1/302). Of the 17 cats infected with FIV, nine (52.94%) were symptomatic. There was a prevalence of FIV infection in males (p 0.0316) and cats aged between one and three years (p 0.0324).

Fracionamento da fertirrigação e seu efeito na produção da alface sob ambiente protegido

Buscando quantificar o efeito do parcelamento da fertirrigação no desenvolvimento da alface, esta pesquisa procurou verificar o número de parcelas de fertirrigação que apresentasse o melhor desempenho no crescimento da cultura. O experimento se deu em blocos casualizados, com três repetições, quatro tratamentos de fertirrigação e três lâminas de irrigação (L1, L2 e L3). Em um dos tratamentos de fertirrigação (F1), toda a adubação necessária se deu no início do plantio; no tratamento F2, a metade da adubação se deu no início do plantio e a outra metade no meio do ciclo da cultura, uma vez planejada a colheita em 40 dias após o plantio; o tratamento F3 foi fracionado: 1/3 da adubação no início do plantio, 1/3 depois de 13 dias e o ultimo terço após 26 dias do plantio; o tratamento F4 seguiu o mesmo raciocínio, fracionando a aplicação em 4 partes, de 10 em 10 dias. Logo, os quatro tratamentos de fertirrigação receberam a mesma dosagem de fertilizantes. Os resultados permitiram concluir que a aplicação F1 resultou no pior desempenho, com massa fresca de 135,06 gramas por planta, enquanto o melhor resultado se deu no tratamento F2, com massa fresca de 161,40 g. Em todas as lâminas, o parcelamento F3 resultou em sistema radicular mais desenvolvido. O tratamento L1 apresentou em média maior desenvolvimento das raízes, seguido do L2 e L3. O tratamento L1 apresentou menor condutividade elétrica no extrato da solução dos vasos, sendo o único tratamento que diferiu estatisticamente dos demais.

Atividade viral do vírus respiratório sincicial bovino (brsv) em bezerros leiteiros

The purpose of the present study was to evaluate the antimicrobial activity of propolis extracts diluted in different solvents against bacteria from Staphylococcus genus. The study was performed in the Immunology and Microbiology Laboratory from Universidade Federal do Vale do São Francisco. The propolis extracts were prepared using brown propolis diluted in different solvents such as chloroform, methanol, ethyl acetate and grain alcohol. In order to determine the antimicrobial potential of extracts, agar well diffusion method was used, with controls for each diluent. After that, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods were used. All tests were performed in triplicate. In the agar well diffusion test, the measurements of the inhibition zone for propolis extract were as follows: grain alcohol and propolis (2.88mm), methanol and propolis (2.41mm), chloroform and propolis (2.40mm) and ethyl acetate and propolis (0.83mm). The MBC of propolis extracts in different solvents were 93.75 μg/mL for grain alcohol, 375 μg/mL for chloroform and methanol and 3,000 μg/ml for ethyl acetate. Statistically significant differences were achieved comparing the inhibition zones of propolis diluted in grain alcohol and ethyl acetate (2.88 and 0.83 mm, respectively). Considering the low cost of therapy and the activity of the propolis against caprine mastitis pathogens, other studies regarding in vivo activity and chemical characterization are necessary, in addition to evaluation of the toxicological aspects of propolis extracts.

Infecção pelo vírus da diarreia viral bovina em vacas gestantes abatidas no Estado de São Paulo-Brasil

The most important pathogens in the bovine livestock nowadays in the virus of the viral diarrhea mainly for triggered clinical manifestations related to the reproductive sphere. The infection in pregnant females, may result in abortions, embryonic resorptions, fetal mummification, birth of weak and malformation of the cattle. Moreover, their birth with persistently infected and immunotolerant virus, which the source of infection and dissemination of their disease. Nowadays, the complexity of the diagnosis and consequently its pathogenesis are tilted in the genotypic differences agent. So, this study aimed to verify the occurrence of the BVDV-1 (SINGER) and BVDV-2 (VS-253) genotypes in cows and their respective fetuses, slaughtered in an abattoir in the State of Sao Paulo. Through blood serum, using virus neutralization technique. All in all, 52,51% (115/219) of the cows which were tested reacted, but no fetus (0/219) reacted, to its virus neutralization. Through this cross-examination we observed that 42% (92/219) of cows reacted for both BVDV-1 and BVDV-2. Furthermore 4,10% (9/219) of them reacted only to the genotype BVDV-1 and 6,39% (14/219) responded only to the genotype 2 of BVDV. Therefore it was noticed that both strains are widespread in the regions studied, which justifies the use of different antigens to avoid false-negative diagnosis. Finally antibodies showed no fetus or fetal abnormalities, it is already developed and can be considered immunocompetent, independent child born to a reagent.

Falhas reprodutivas associadas com a presença de do vírus da artrite-encefalite caprina, toxoplasma gondii e neospora caninum em caprinos no estado de São Paulo, Brasil

This study aimed at assessing the occurrence of antibodies against the caprine arthritis-encephalitis virus (CAEV), Toxoplasma gondii and Neospora caninum, as well as the associations between the presence of antibodies and the occurrence of reproductive failures in goats. Serum samples were collected from 923 goats of both sexes, over 3 months of age, from 17 dairy farms located in different municipalities of São Paulo State, Brazil. Infections by T. gondii, N. caninum and CAEV were evaluated by indirect methods of diagnosis based on indirect fluorescence antibody test (IFAT), Neospora agglutination test (NAT), and agar gel immunodiffusion (AGID), respectively. A survey was conducted on the farms to obtain information about reproduction dates (abortions, stillbirths and births of weak and premature kids) and zoosanitary management. Antibodies against CAEV, T. gondii and N. caninum was found in 37.81%, 23.62% and 17.23% respectively. There was no significant association between the presence of anti-CAEV antibodies and CAEV/T. gondii or CAEV/N. caninum co-infection, suggesting that CAEV does not predispose goats to infection by these agents. However, when CAEV/T. gondii (p<0.01) or CAEV/N. caninum (p<0.001) co-infection was present, the occurrence of reproductive failures was significantly higher what could indicate that CAEV-induced immunosuppression may predispose goats to develop the clinical symptoms of toxoplasmosis and neosporosis increasing the risks of the reproductive failures.

Caracterização genética do vírus da diarreia bovina detectada em bovinos do Sul de Minas Gerais, Brasil, persistentemente infectados

Isolates of bovine viral diarrhoea virus (BVDV) detected in serum samples of two persistently infected animals (PI) identified in a herd located in the southern state of Minas Gerais, Brazil, underwent genetic characterization trough partial nucleotide sequencing and analysis of the 5' Untranslated Region (5'UTR) of the viral genome. The isolates were characterized as belonging to genotype BVDV-1, subgenotype BVDV-1b. The results of this study suggest BVDV-1b as an agent of importance in the occurrence of bovine viral diarrhoea (BVD) in the herds of the region. Moreover, the genotypic characterization of isolates of BVDV helps to better understand the epidemiology of the disease, as the genetic variability of BVDV interferes in the serological tests and has implications for the use of vaccines, whose majority is produced only with reference strains of BVDV. Therefore, the investigation on the genetic diversity of BVDV existing in Brazil is required for the improvement of the disease prevention and control measures.

Resposta da alface à aplicação de biofertilizante sob dois níveis de irrigação

The use of biofertilizers is interesting for agriculture as being an economical alternative as well as it is environmentally friendly by using organic waste and reducing the application of mineral fertilizers. The aim of this study was to evaluate the effect of biofertilizer doses of bovine origin (biodigester effluent) applied on the ground and two levels of irrigation on lettuce. The experiment was conducted under protection of a greenhouse in pots, applying to the soil different doses of biofertilizer of bovine origin obtained from anaerobic reactor (10, 20, 40 and 60 m3 ha-1) and mineral fertilizer as a witness in two irrigation levels calculated at 50 and 100% of reference evapotranspiration. The lettuce plants were analyzed in their: Height, leaves number, crown diameter, fresh weight and dry weight of shoots. The biofertilizer treatments showed better results than the mineral fertilizer and has increased with increasing doses of biofertilizer, the highest dose (60 m3 ha-1) showed the best results in all variables. For dry, mineral fertilization showed higher values. The irrigation levels had no effect on plant growth.

Co-infecção por vírus dengue, sorotipos 1 e 4, em paciente de cidade de porte médio no Brasil

The natural co-infection with dengue virus can occur in highly endemic areas where different serotypes have been observed for many years. We report one case of DENV-1/DENV-4 co-infection in human serum detected by molecular tests. Phylogenetic analysis of the sequences obtained indicated the presence of genotype V and II for DENV-1 and DENV-4, respectively.