Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain

Although long suspected from histochemical evidence for carbonic anhydrase (CA) activity on neurons and observations that CA inhibitors enhance the extracellular alkaline shifts associated with synaptic transmission, an extracellular CA in brain had not been identified. A candidate for this CA was suggested by the recent discovery of membrane CA (CA XIV) whose mRNA is expressed in mouse and human brain and in several other tissues. For immunolocalization of CA XIV in mouse and human brain, we developed two antibodies, one against a secretory form of enzymatically active recombinant mouse CA XIV, and one against a synthetic peptide corresponding to the 24 C-terminal amino acids in the human enzyme. Immunostaining for CA XIV was found on neuronal membranes and axons in both mouse and human brain. The highest expression was seen on large neuronal bodies and axons in the anterolateral part of pons and medulla oblongata. Other CA XIV-positive sites included the hippocampus, corpus callosum, cerebellar white matter and peduncles, pyramidal tract, and choroid plexus. Mouse brain also showed a positive reaction in the molecular layer of the cerebral cortex and granular cellular layer of the cerebellum. These observations make CA XIV a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Mouse strain differences determine severity of iron accumulation in Hfe knockout model of hereditary hemochromatosis

Hereditary hemochromatosis (HH) is a common disorder of iron metabolism caused by mutation in HFE, a gene encoding an MHC class I-like protein. Clinical studies demonstrate that the severity of iron loading is highly variable among individuals with identical HFE genotypes. To determine whether genetic factors other than Hfe genotype influence the severity of iron loading in the murine model of HH, we bred the disrupted murine Hfe allele onto three different genetically defined mouse strains (AKR, C57BL/6, and C3H), which differ in basal iron status and sensitivity to dietary iron loading. Serum transferrin saturations (percent saturation of serum transferrin with iron), hepatic and splenic iron concentrations, and hepatocellular iron distribution patterns were compared for wild-type (Hfe +/+), heterozygote (Hfe +/−), and knockout (Hfe −/−) mice from each strain. Although the Hfe −/− mice from all three strains demonstrated increased transferrin saturations and liver iron concentrations compared with Hfe +/+ mice, strain differences in severity of iron accumulation were striking. Targeted disruption of the Hfe gene led to hepatic iron levels in Hfe −/− AKR mice that were 2.5 or 3.6 times higher than those of Hfe −/− C3H or Hfe −/− C57BL/6 mice, respectively. The Hfe −/− mice also demonstrated strain-dependent differences in transferrin saturation, with the highest values in AKR mice and the lowest values in C3H mice. These observations demonstrate that heritable factors markedly influence iron homeostasis in response to Hfe disruption. Analysis of mice from crosses between C57BL/6 and AKR mice should allow the mapping and subsequent identification of genes modifying the severity of iron loading in this murine model of HH.

Kikulacho [videorecording] =: The bite within

This documentary shows how Kenyan leaders are to blame for the social injustices suffered by Kenyans since the attainment of independence from the British. The issue of land and land clashes, corruption, irregularities in elections and the constitution are some of the highlights of this documentary.

Beyond the plains [videorecording]: histoire exemplaire d'un africain ordinaire

Shows the traditional world of the Masai as it is confronted by the westernized, modern world by telling the life story of a young Masai. Dramatized scenes show his early life in a village, his school days and his present position as a university lecturer.

Hikayat Raja Kulawandu : manuscript, 1838

Title from f. 1r.

Hikayat Darma Taʻsia : manuscript, 1838

According to the colophon (f. 68r), copy completed in Dhū al-Ḥijjah 1253 [February 1838] in the hand of Muhammad Ali bin Abdul Latif Munsyi.

Miocene microflora and palaeoclimate reconstructions from three sites in Bulgaria

In this study we reconstruct quantitatively the Middle to Upper Miocene climate evolution in the southern Forecarpathian Basin (Central Paratethys area, Northwest Bulgaria) by applying the coexistence approach to 101 well-dated palynofloras isolated from three cores. The climatic evolution is compared with changes in vegetation and palaeogeography. The Middle Miocene was a period of a subtropical/warm-temperate humid climate with mean annual temperature (MAT) between 16 and 18°C and mean annual precipitation (MAP) between 1100 and 1300 mm. Thereby, during the entire Middle Miocene a trend of slightly decreasing temperatures is observed and only small climate fluctuations occur which are presumably related to palaeogeographic reorganisations. The vegetation shows a corresponding trend with a decrease in abundance of palaeotropic and thermophilous elements. The Upper Miocene is characterised by more diverse climatic conditions, probably depending on palaeogeographic and global climatic transformations. The beginning of this period is marked by a slight cooling and a significant drying of the climate, with MAT 13.3-17°C and MAP 652-759 mm. After that, fluctuations of all palaeoclimate parameters occur displaying cycles of humid/dryer and warmer/cooler conditions, which are again well reflected in the vegetation. Our study provides a first quantitative model of the Middle-Upper Miocene palaeoclimate evolution in Southeastern Europe and is characterised by a relatively high precision and resolution with respect to the climate data and stratigraphy.

Improving the performance of virtual memory computers /

"UILU-ENG 78 1741."

Some results on the working set anomalies in numerical programs /

"UILU-ENG 80 1744"--Cover.

Program behavior under VAX/VMS /

"UILU-ENG 83 1727"--Cover.

Technical education & its need in India.

Letter concerning authorship, tipped in.

Die pharmakologischen Grundsätze (Liber fundamentorum pharmacologiae)

Reprinted from the Historische Studien of the Pharmakologisches Institut, Universität Dorpat, Bd. 3, 1893.