Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase

tRNAs are charged with cognate amino acids by aminoacyl-tRNA synthetases (aaRSs) and subsequently delivered to the ribosome to be used as substrates for gene translation. Whether aminoacyl-tRNAs are channeled to the ribosome by transit within translational complexes that avoid their diffusion in the cytoplasm is a matter of intense investigation in organisms of the three domains of life. In the cyanobacterium Anabaena sp. PCC 7120, the valyl-tRNA synthetase (ValRS) is anchored to thylakoid membranes by means of the CAAD domain. We have investigated whether in this organism ValRS could act as a hub for the nucleation of a translational complex by attracting other aaRSs to the membranes. Out of the 20 aaRSs, only ValRS was found to localize in thylakoid membranes whereas the other enzymes occupied the soluble portion of the cytoplasm. To investigate the basis for this asymmetric distribution of aaRSs, a global search for proteins interacting with the 20 aaRSs was conducted. The interaction between ValRS and the FoF1 ATP synthase complex here reported is of utmost interest and suggests a functional link between elements of the gene translation and energy production machineries.

The Boston Marathon and the Visa Negotiations between the EU and Russia: Time to get real and positive. CEPS Commentary, 8 May 2013

Against the background of the Boston Marathon bombing as a violent reminder of the lawlessness throughout much of the Northern Caucasus, Michael Emerson calls in this commentary for a fresh direction in the EU’s negotiations with Russia over visa-free travel. While finding that a ‘visa liberalisation’ regime has a very slim prospect of materialising any time soon, on the other hand, he sees realistic possibilities for fast delivery of a ‘virtual visa free regime’ of the multi-year, multi-entry visa, which would be a real and important achievement for EU-Russian relations.

Interstate Commerce Commission: impacts of eliminating or transferring motor carrier and other functions. Statement of Barry T. Hill.

Mode of access: Internet.

A compendium of the law of real and personal property : connected with conveyancing, for the use of students and practitioners /

Includes bibliographical footnotes and index.

Tables of the common logarithms and trigonometrical functions to six places of decimals.

"With an appendix containing a table of natural functions and circular measures of angles to each minute of arc to five places of decimals."

The statistical estimation of throughput and turnaround functions for a university computer system /

Vita.

Notebook in extension history, objectives and staff functions, developed in special summer school courses at Oklahoma Agricultural and Mechanical College, Stillwater, Oklahoma, June 6-25, 1955. Karl Knaus, Instructor.

Mode of access: Internet.

Basic mathematics in electrical communications. A treatise on conic section curves, exponentials, alternating current, electrical oscillations, transients and hyperbolic functions.

Mode of access: Internet.

Diencephalon, autonomic and extrapyramidal functions

Mode of access: Internet.

A century of gossip, or, The real and the seeming /

At head of title: New England life.

Margaret: a tale of the real and the ideal, blight and bloom; including sketches of a place not before described, called Mons Christi ...

Mode of access: Internet.

Logarithmic tables of numbers and trigonometrical functions /

Mode of access: Internet.

Food and its functions, a text-book for students of cookery.

Mode of access: Internet.

Logarithmic tables of numbers and trigonometrical functions,

Mode of access: Internet.

Simple and complex models for nonlinear seismic response of reinforced concrete structures /

Mode of access: Internet.