At least two indigenous species of the Bemisia tabaci complex are present in Brazil

Bemisia tabaci is one of the most important global agricultural insect pests, being a vector of emerging plant viruses such as begomoviruses and criniviruses that cause serious problems in many countries. Although knowledge of the genetic diversity of B. tabaci populations is important for controlling this pest and understanding viral epidemics, limited information is available on this pest in Brazil. A survey was conducted in different locations of São Paulo and Mato Grosso states, and the phylogenetic relationships of B. tabaci individuals from 43 populations sampled from different hosts were analysed based on partial mitochondrial cytochrome oxidase 1 gene (mtCOI) sequences. According to the recently proposed classification of the B. tabaci complex, which employs the 3.5% mtCOI sequence divergence threshold for species demarcation, most of the specimens collected were found to belong to the Middle East-Asia Minor 1 species, which includes the invasive populations of the commonly known B biotype, within the Africa/Middle East/Asia Minor high-level group. Three specimens collected from Solanun gilo and Ipomoea sp. were grouped together and could be classified in the New World species that includes the commonly known A biotype. However, six specimens collected from Euphorbia heterophylla, Xanthium cavanillesii and Glycine maxima could not be classified into any of the 28 previously proposed species, although according to the 11% mtCOI sequence divergence threshold, they belong to the New World high-level group. These specimens were classified into a new recently proposed species named New World 2 that includes populations from Argentina. Middle East-Asia Minor 1, New World and New World 2 were differentiated by RFLP analysis of the mtCOI gene using TaqI enzyme. Taq I analysis in silico also differentiates these from Mediterranean species, thus making this method a convenient tool to determine population dynamics, especially critical for monitoring the presence of this exotic pest in Brazil. © 2012 Blackwell Verlag, GmbH.

América Latina: la transición demográfica en sectores rezagados

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Efectos contextuales y fecundidad marital: un modelo de niveles múltiples de la paridez en la región del Bío-Bío, Chile

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Sobre la "migratología"

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México: estimación de la mortalidad materna a partir de diversas fuentes

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Extensión del método del hijo previo

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Pareo de datos sobre defunciones infantiles y nacimientos en Chile

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El uso de variables sintomáticas en la actualización de población de áreas menores

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La evolución del divorcio en Uruguay, 1950-1995

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Condición migratoria, raza y género en el mercado de trabajo brasileño: el caso de las regiones metropolitanas de Río de Janeiro y Sao Paulo

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Magnetic field profile of a mesoscopic SQUID-shaped superconducting film

Using a genuinely tridimensional approach to the time-dependent Ginzburg-Landau theory, we have studied the local magnetic field profile of a mesoscopic superconductor in the so-called SQUID geometry, i.e., a square with a hole at the center connected to the outside vacuum through a very thin slit. Our investigation was carried out in both the Meissner and the mixed state. We have also studied the influence of the temperature on the space distribution of the local magnetic field. © 2013 IOP Publishing Ltd.

Extracellular β-fructofuranosidase from Fusarium graminearum: Stability of the spray-dried enzyme in the presence of different carbohydrates

Microbial enzymes have been used for various biotechnological applications; however, enzyme stabilization remains a challenge for industries and needs to be considered. This study describes the effects of spray-drying conditions on the activity and stability of β-fructofuranosidase from Fusarium graminearum. The extracellular enzyme β-fructofuranosidase was spray dried in the presence of stabilizers, including starch (Capsul) (SC), microcrystalline cellulose (MC), trehalose (TR), lactose (LC) and β-cyclodextrin (CD). In the presence of TR (2% w/v), the enzymatic activity was fully retained. After 1 year of storage, 74% of the enzymatic activity was maintained with the CD stabilizer (10% w/v). The residual activity was maintained as high as 80% for 1 h at 70°C when MC, SC and CD (5% w/v) stabilizers were used. Spray drying with carbohydrates was effective in stabilizing the F. graminearum β-fructofuranosidase, improved enzymatic properties compared to the soluble enzyme and demonstrated a potential use in future biotechnology applications. © 2013 Informa UK Ltd. All rights reserved.

Banco de coyuntura: series trimestrales y mensuales del producto interno bruto (PIB), III-2000

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