Comparison of Brazilian plants used to treat gastritis on the oxidative burst of helicobacter pylori -stimulated neutrophil

Ten Brazilian medicinal plants used to treat gastritis and ulcers were carefully selected on the basis of ethnopharmacological importance and antiulcerogenic activity previously described. The antioxidant activity of the methanolic extracts was determined in analysis conditions that simulate a real biological activity on inhibition of the oxidative burst induced in neutrophils using Helicobacter pylori as activator, by a luminol-amplified chemiluminescence assay. The extracts, at low concentration (5 g/mL), exhibited a large variation in inhibitory effects of H. pylori-induced oxidative burst ranging from 48% inhibition to inactive, but all extracts, excluding Byrsonima intermedia, had inhibitory activity over 80% at the concentration of 100 g/mL. The total suppressive antioxidant capacity measured as the effective concentration, which represents the extract concentration producing 50% inhibition of the chemiluminescence induced by H. pylori, varies from 27.2 to 56.8 g/mL and was in the following order: Qualea parviflora > Qualea multiflora > Alchornea triplinervia > Qualea grandiflora > Anacardium humile > Davilla elliptica > Mouriri pusa > Byrsonima basiloba > Alchornea glandulosa > Byrsonima intermedia. The main groups of compounds in tested extracts are presented. Differences in the phytochemical profile, quantitatively and qualitatively, of these plants can explain and justify their protective effect on the gastric mucosa caused by the neutrophil-generated ROS that occurs when H. pylori displays its evasion mechanisms. © 2013 Cibele Bonacorsi et al.

Leishmaniasis causes oxidative stress and alteration of oxidative metabolism and viability of neutrophils in dogs

The aim of the present study was to test the hypothesis that oxidative stress and alteration of oxidative metabolism and apoptosis of neutrophils in dogs vary with the stage of leishmaniasis and to determine the contribution of uremia to such alterations. Dogs with leishmaniasis were classified into two stages: moderate (Leish II, n = 20) or very severe (i.e. with concurrent uremia; Leish IV, n = 20) according to the LeishVet Consensus. The two leishmaniasis groups were compared with uremic dogs without leishmaniasis (Uremic, n = 10) and to healthy dogs (Control, n = 30). To determine oxidative stress, total antioxidant/oxidant capacity, lipid peroxidation, total glutathione and the plasma antioxidants albumin, uric acid and bilirubin were quantified. Superoxide production was determined using the hydroethidine probe and viability and apoptosis were measured using annexin V-PE by capillary flow cytometry. Oxidative stress was present in both uremia and leishmaniasis with reduced total antioxidant capacity and was associated with increased induced production of superoxide and apoptosis. The greatest amount of oxidants was observed in animals with moderate disease only. Neutrophils from uremic dogs with and without leishmaniasis had decreased viability and an increased apoptosis rate in addition to increased lipid peroxidation. In conclusion, oxidative stress occurs in both stages of leishmaniasis with differences in intensity and levels of plasma markers; however, uremia does contribute to the decreased spontaneous viability of neutrophils in dogs in the final stage of the disease. © 2013 Elsevier Ltd. All rights reserved.

Sistema com aeração, decantação e filtragem para melhoria da qualidade da água para irrigação localizada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Propriedades funcionais da carne de ratitas: ema (Rhea americana) e avestruz (Struthio camellus) comparadas com a carne de frango (Gallus domesticus)

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Aplicação de 1-mcp na conservação de abacate ‘Hass’

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Uniformidade de distribuição de água em irrigação localizada com sistema de aeração, decantação e filtragem

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Engenharia Civil e Ambiental - FEB

Uso e ocupação do solo e monitoramento dos recursos hídricos na microbacia do Córrego do Ipê, Ilha Solteira, SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Agronomia - FEIS

Diagnóstico dos recursos hídricos na microbacia do Córrego Três Barras, Marinópolis, SP

Pós-graduação em Agronomia - FEIS

Qualidade de água para a irrigação na microbacia do córrego Três Barras no município de Marinópolis, SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade da água para irrigação na microbacia do Córrego do Coqueiro no Noroeste Paulista

Pós-graduação em Agronomia - FEIS

Metabolismo do ferro e lipoperoxidação eritrocitária em equinos puro sangue árabe sumetidos ao exercício em esteira e suplementados com vitamina E (dl-alfa-tocoferol)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Capacidade antioxidante na Hb S: influência de talassemia alfa, haplótipos do gene βS globina e melatonina

Pós-graduação em Genética - IBILCE