Functional studies of {\it twist\/} during mouse embryogenesis

A fundamental task in developmental biology is to understand the molecular mechanisms governing early embryogenesis. The aim of this study was to understand the developmental role of a putative basic helix-loop-helix (b-HLH) transcription factor, twist, during mouse embryogenesis.^ twist was originally identified in Drosophila as one of the zygotic genes, including snail, that were required for dorsal-ventral patterning. In Drosophila embryogenesis, twist is expressed in the cells of the ventral midline destined to form mesoderm. In embryos lacking twist expression, their ventral cells fail to form a ventral furrow and subsequently no mesoderm is formed.^ During mouse embryogenesis, twist is expressed after initial mesoderm formation in both mesoderm and cranial neural crest cell derivatives. To study the role of twist in vivo, twist-null embryos were generated by gene targeting. Embryos homozygous for the twist mutation die at midgestation. The most prominent phenotype in the present study was a failure of the cranial neural tube to close (exencephaly). twist-null embryos also showed defects in head mesenchyme, branchial arches, somites, and limb buds.^ To understand whether twist functions cell-autonomously and to investigate how twist-null cells interact with wild-type cells in vivo, twist chimeras composed of both twist-null and wild-type cells marked by the expression of the lacZgene were generated. Chimeric analysis revealed a correlation between the incidence of exencephaly and the contribution of the underlying twist-null head mesenchyme, thus strongly suggesting that twist-expressing head mesenchyme is required for the closure of the cranial neural tube. These studies have identified twist as a critical regulator for the mesenchymal fate determination within the cranial neural crest lineage. Most strikingly, twist-null head mesenchyme cells were always segregated from wild-type cells, indicating that the twist mutation altered the adhesive specificity of these cells. Furthermore, these results also indicated that twist functions cell-autonomously in the head, arch, and limb mesenchyme but non-cell-autonomously in the somites. Taken together, these studies have established the essential role of twist during mouse embryogenesis. ^

Patrón génico de fibrosis y apoptosis en nefropatía obstructiva experimental : : modulación por rosuvastatina

La nefropatía obstructiva puede ser un desorden renal complejo de tratar debido al severo cuadro inflamatorio, desbalance oxidativo, apoptosis y fibrosis. Estudios previos sostienen que rosuvastatina (Ros) podría tener utilidad como una opción terapéutica en enfermedades renales que cursarían con apoptosis y fibrosis. Objetivo: Evaluar los posibles efectos antiapoptóticos y antifibróticos de Ros durante la obstrucción ureteral unilateral en ratas neonatas. Materiales y Métodos: Ratas Wistar neonatas de 48 hs. de vida fueron intervenidas quirúrgicamente (grupo experimental) o no (grupo control). Ambos grupos fueron subdivididos en tratadas o no tratadas con Ros (10mg / kg por día) vía oral durante 14 días. Posteriormente se procedió a nefrectomizar y procesar las cortezas renales para determinar por RT-PCR las expresiones de genes: óxido nítrico sintasa inducible (iNOS), factor promotor génico de chaperonas (hsf1), proteína de shock térmico (hsp70), bax, bcL2, wt1, p53, snail, proteína morfogénica del hueso (bmp7), caderina E, factor transformador de crecimiento (tgf-β) y factor de necrosis tumoral (tnf-α). Resultados: La obstrucción ureteral unilateral neonatal indujo una marcada fibrosis y apoptosis, mientras que el tratamiento con Ros moduló el patrón de genes fibróticos y apoptóticos mediante disminución de la expresión de bmp7, caderina E, wt1, p53 y bcl2; además indujo una caída en la expresión de los genes profibróticos y proapoptóticos (bax, tnf-α y tgf-β). El análisis de los resultados presentados, permiten sugerir que la protección renal de rosuvastatina durante nefropatía obstructiva de ratas neonatas estaría asociado a la interacción entre hsp70 y la biodisponibilidad del óxido nítrico con el concomitante descenso en genes pro-apoptóticos.

"La estrategia del caracol". El Neozapatismo : Insurgencia indígena y desobediencia civil

A la luz de sus autodefiniciones, se puede advertir el devenir del movimiento zapatista desde una organización indígena-campesina, democrática -y de tinte "multicultural"-, a una fuerza indígena-popular de carácter democrático y anticapitalista. La característica que especifica al movimiento es el haber hecho del problema de "los pueblos indios" una cuestión social y política al mismo tiempo, sin reducirla a un conflicto jurídico-político con el Estado. En el marco de los debates y polémicas -académicas y políticas- generados en los últimos años en torno a este devenir, nos propusimos explorar el proceso, enfocándonos en las relaciones entre las comunidades zapatistas en movimiento, y la sociedad civil mexicana

Entrelíneas para una introspección poética : Rubén Darío y Antonio Machado en 1903

Este trabajo intenta mostrar una actitud poética común coincidente entre Antonio Machado y Rubén Darío. Dicha actitud, que consiste en defender la introspección como fórmula adecuada para la expresión poética, es perceptible en un momento determinado de la trayectoria poética individual de cada uno. Sin embargo, es interesante ver cómo se muestra en el diálogo estético que ambos establecen a través de su creación como ofrenda al otro: aquellos poemas en los que el paratexto se dedica al otro pero el texto no se adecua (no utilizan el ritmo, el tono y el léxico) a quien va dirigida la dedicatoria. Obras que son parte de uno mismo, canto a sí mismo, y, sin embargo, se ofrecen al otro. Esta ofrenda puede apreciarse, desde nuestra perspectiva, en dos poemas concretos: "El canto de los niños", y "Caracol". Dedicados del uno al otro, son analizados en coincidencia estética entre Darío y Machado en torno a la fecha de 1903.

"La estrategia del caracol". El Neozapatismo : Insurgencia indígena y desobediencia civil

A la luz de sus autodefiniciones, se puede advertir el devenir del movimiento zapatista desde una organización indígena-campesina, democrática -y de tinte "multicultural"-, a una fuerza indígena-popular de carácter democrático y anticapitalista. La característica que especifica al movimiento es el haber hecho del problema de "los pueblos indios" una cuestión social y política al mismo tiempo, sin reducirla a un conflicto jurídico-político con el Estado. En el marco de los debates y polémicas -académicas y políticas- generados en los últimos años en torno a este devenir, nos propusimos explorar el proceso, enfocándonos en las relaciones entre las comunidades zapatistas en movimiento, y la sociedad civil mexicana

Entrelíneas para una introspección poética : Rubén Darío y Antonio Machado en 1903

Este trabajo intenta mostrar una actitud poética común coincidente entre Antonio Machado y Rubén Darío. Dicha actitud, que consiste en defender la introspección como fórmula adecuada para la expresión poética, es perceptible en un momento determinado de la trayectoria poética individual de cada uno. Sin embargo, es interesante ver cómo se muestra en el diálogo estético que ambos establecen a través de su creación como ofrenda al otro: aquellos poemas en los que el paratexto se dedica al otro pero el texto no se adecua (no utilizan el ritmo, el tono y el léxico) a quien va dirigida la dedicatoria. Obras que son parte de uno mismo, canto a sí mismo, y, sin embargo, se ofrecen al otro. Esta ofrenda puede apreciarse, desde nuestra perspectiva, en dos poemas concretos: "El canto de los niños", y "Caracol". Dedicados del uno al otro, son analizados en coincidencia estética entre Darío y Machado en torno a la fecha de 1903.

"La estrategia del caracol". El Neozapatismo : Insurgencia indígena y desobediencia civil

A la luz de sus autodefiniciones, se puede advertir el devenir del movimiento zapatista desde una organización indígena-campesina, democrática -y de tinte "multicultural"-, a una fuerza indígena-popular de carácter democrático y anticapitalista. La característica que especifica al movimiento es el haber hecho del problema de "los pueblos indios" una cuestión social y política al mismo tiempo, sin reducirla a un conflicto jurídico-político con el Estado. En el marco de los debates y polémicas -académicas y políticas- generados en los últimos años en torno a este devenir, nos propusimos explorar el proceso, enfocándonos en las relaciones entre las comunidades zapatistas en movimiento, y la sociedad civil mexicana

Entrelíneas para una introspección poética : Rubén Darío y Antonio Machado en 1903

Este trabajo intenta mostrar una actitud poética común coincidente entre Antonio Machado y Rubén Darío. Dicha actitud, que consiste en defender la introspección como fórmula adecuada para la expresión poética, es perceptible en un momento determinado de la trayectoria poética individual de cada uno. Sin embargo, es interesante ver cómo se muestra en el diálogo estético que ambos establecen a través de su creación como ofrenda al otro: aquellos poemas en los que el paratexto se dedica al otro pero el texto no se adecua (no utilizan el ritmo, el tono y el léxico) a quien va dirigida la dedicatoria. Obras que son parte de uno mismo, canto a sí mismo, y, sin embargo, se ofrecen al otro. Esta ofrenda puede apreciarse, desde nuestra perspectiva, en dos poemas concretos: "El canto de los niños", y "Caracol". Dedicados del uno al otro, son analizados en coincidencia estética entre Darío y Machado en torno a la fecha de 1903.

Avian species identity and predation success in tropical cacao agroforestry of the Napu Valley in Central Sulawesi, Indonesia

Avian ecosystem services such as the suppression of pests are considered being of high ecological and economic importance in a range of ecosystems, especially in tropical agroforestry. But how bird predation success is related to the diversity and composition of the bird community, as well as local and landscape factors, is poorly understood. The author quantified arthropod predation in relation to the identity and diversity of insectivorous birds, using experimental exposure of artificial, caterpillar-like prey on smallholder cacao agroforestry systems, differing in local shade management and distance to primary forest. The bird community was assessed using both mist netting (targeting on active understory insectivores) and point count (higher completeness of species inventories) sampling. The study was conducted in a land use dominated area in Central Sulawesi, Indonesia, adjacent to the Lore Lindu National Park. We selected 15 smallholder cacao plantations as sites for bird and bat exclosure experiments in March 2010. Until July 2011, we recorded several data in this study area, including the bird community data, cacao tree data and bird predation experiments that are presented here. We found that avian predation success can be driven by single and abundant insectivorous species, rather than by overall bird species richness. Forest proximity was important for enhancing the density of this key species, but did also promote bird species richness. The availability of local shade trees had no effects on the local bird community or avian predation success. Our findings are both of economical as well as ecological interest because the conservation of nearby forest remnants will likely benefit human needs and biodiversity conservation alike.

Seawater carbonate chemistry and Littorina obtusata biological processes during experiments, 2009

Our understanding of the effects of ocean acidification on whole organism function is growing, but most current information is for adult stages of development. Here, we show the effects of reduced pH seawater (pH 7.6) on aspects of the development, physiology and behaviour of encapsulated embryos of the marine intertidal gastropod Littorina obtusata. We found reduced viability and increased development times under reduced pH conditions, and the embryos had significantly altered behaviours and physiologies. In acidified seawater, embryos spent more time stationary, had slower rotation rates, spent less time crawling, but increased their movement periodicity compared with those maintained under control conditions. Larval and adult heart rates were significantly lower in acidified seawater, and hatchling snails had an altered shell morphology (lateral length and spiral shell length) compared to control snails. Our findings show that ocean acidification may have multiple, subtle effects during the early development of marine animals that may have implications for their survival beyond those predicted using later life stages.

Multiple stressor effects of near-future elevated seawater temperature and decreased pH on righting and escape behaviors of two common Antarctic gastropods

Warming seawater temperatures and ocean acidification on the coastal western Antarctic Peninsula pose unique challenges to stenothermal marine invertebrates. The present study examines prospective sub-lethal effects of elevated temperature, pCO2, and resultant decrease in seawater pH, on righting behavior and maximal escape speeds for two common gastropods, the limpet Nacella concinna (Strebel) and mesogastropod snail Margarella antarctica (Lamy). Replicate individuals held in individual containers were exposed to four combinations of seawater temperature (1.5 °C-current average, 3.5 °C-projected average by 2100) and pH (pH 8.0-current average, pH 7.8-projected average by 2100 as a result of elevated pCO2 levels) for a period of 6 weeks. Following this chronic exposure, righting behavior, determined for the limpets as proportion to right over 24 h and for snails as time to right, as well as maximum escape speed following contact with a sea star predator were measured. We found no significant differences in proportions of limpets displaying the capacity to right among the four temperature-pH treatments. However, there was a significant temperature-pH interaction effect for mean righting times in snails, indicating that the effect of pH on the time to right is dependent on temperature. We found no significant effects of temperature or pH on mean maximal escape speed in limpets. Additionally, we observed a significant temperature-pH interaction effect for mean maximal escape speed in snails. These interactive effects make it difficult to make clear predictions about how these environmental factors may impact behavioral responses.

Impacts of ocean acidification on multiplication and caste organisation of parasitic trematodes in their gastropod host

Ocean acidification is predicted to impact the structure and function of all marine ecosystems in this century. As focus turns towards possible impacts on interactions among marine organisms, its effects on the biology and transmission potential of marine parasites must be evaluated. In the present study, we investigate two marine trematode species (Philophthalmus sp. and Parorchis sp., both in the family Philophthalmidae) infecting two marine gastropods. These trematodes are unusual in that their asexually multiplying stages within snails display a division of labour, with two distinct castes, a large-bodied morph producing infective stages and a smaller morph playing a defensive role against other competing parasites. Using a potentiometric ocean acidification simulation system, we test the impacts of acidified seawater (7.8 and 7.6 pH) on the production of free-living infective stages (cercariae), the size and survival of encysted resting stages (metacercariae), and the within-host division of labour measured as the ratio between numbers of the two morphs. In general, low pH conditions caused an increase in cercarial production and a reduction in metacercarial survival. The ratio of the two castes within snail hosts tended to shift towards more of the smaller defensive morphs under low pH. However, the observed effects of reduced pH were species specific and not always unimodal. These results suggest that ocean acidification can affect the biology of marine parasites and may also impact transmission success and parasite abundance of some trematodes, with possible consequences for marine communities and ecosystems.

Geographical variation in shell morphology of juvenile snails (Concholepas concholepas) along the physical-chemical gradient of the Chilean coast

Changes in phenotypic traits, such as mollusc shells, are indicative of variations in selective pressure along environmental gradients. Recently, increased sea surface temperature (SST) and ocean acidification (OA) due to increased levels of carbon dioxide in the seawater have been described as selective agents that may affect the biological processes underlying shell formation in calcifying marine organisms. The benthic snail Concholepas concholepas (Muricidae) is widely distributed along the Chilean coast, and so is naturally exposed to a strong physical-chemical latitudinal gradient. In this study, based on elliptical Fourier analysis, we assess changes in shell morphology (outlines analysis) in juvenile C. concholepas collected at northern (23°S), central (33°S) and southern (39°S) locations off the Chilean coast. Shell morphology of individuals collected in northern and central regions correspond to extreme morphotypes, which is in agreement with both the observed regional differences in the shell apex outlines, the high reclassification success of individuals (discriminant function analysis) collected in these regions, and the scaling relationship in shell weight variability among regions. However, these extreme morphotypes showed similar patterns of mineralization of calcium carbonate forms (calcite and aragonite). Geographical variability in shell shape of C. concholepas described by discriminant functions was partially explained by environmental variables (pCO2, SST). This suggests the influence of corrosive waters, such as upwelling and freshwaters penetrating into the coastal ocean, upon spatial variation in shell morphology. Changes in the proportion of calcium carbonate forms precipitated by C. concholepas across their shells and its susceptibility to corrosive coastal waters are discussed.

A family of fibrinogen-related proteins that precipitates parasite-derived molecules is produced by an invertebrate after infection

After infection with the digenetic trematode Echinostoma paraensei, hemolymph of the snail Biomphalaria glabrata contains lectins comprised of 65-kDa subunits that precipitate polypeptides secreted by E. paraensei intramolluscan larvae. Comparable activity is lacking in hemolymph of uninfected snails. Three different cDNAs with sequence similarities to peptides derived from the 65-kDa lectins were obtained and unexpectedly found to encode fibrinogen-related proteins (FREPs). These FREPs also contained regions with sequence similarity to Ig superfamily members. B. glabrata has at least five FREP genes, three of which are expressed at increased levels after infection. Elucidation of components of the defense system of B. glabrata is relevant because this snail is an intermediate host for Schistosoma mansoni, the most widely distributed causative agent of human schistosomiasis. These results are novel in suggesting a role for invertebrate FREPs in recognition of parasite-derived molecules and also provide a model for investigating the diversity of molecules functioning in nonself-recognition in an invertebrate.

TAFII mutations disrupt Dorsal activation in the Drosophila embryo

In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. In vitro transcription reactions and protein binding assays implicate the TAFII110 and TAFII60 subunits of the TFIID complex in contributing to Dorsal-mediated activation. Mutations in TAFII110 and TAFII60 result in altered patterns of snail and twist transcription in embryos derived from dl/+ females. These results suggest that TAFIIs contribute to the activation of transcription in vivo and support the hypothesis that subunits of TFIID may serve as targets of enhancer binding proteins.