Snout mites from caves in Brazil, with description of a new species (Acari: Trombidiformes: Bdellidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

TOURISM IN CAVES and THE CONSERVATION of THE SPELEOLOGICAL HERITAGE: THE CASE of SERRA da BODOQUENA (MATO GROSSO do SUL STATE, BRAZIL)

Heros Augusto Santos Lobo & Edvaldo Cesar Moretti: Tourism in Caves and the Conservation of the Speleological Heritage: The case of Serra da Bodoquena (Mato Grosso do Sul State, Brazil)The Serra da Bodoquena is the region in the state of Mato Grosso do Sul, Brazil in which the Municipality of Bonito is located. This municipality is the primary calling card for tourism in the state and is one of the most developed areas of ecotourism and speleotourism in the country The tourism there is entitled ecotourism, and is designed to be sustainable. The present case study focuses on the ecologically Sustainable aspects of the spleotourism practiced there, especially the proposals for tourist carrying capacity adopted. The results and discussion provide Suggestions for the adoption of a different formulation of carrying capacity focusing on both operational and quantitative aspects. Ecologically Sustainable speleotourism in the Serra da Bodoquena should be possible as long as new proposals limiting visitation are adopted which conform to technical environmental management procedures and consider the interests of local stakeholders.

Paracoccidioides brasiliensis antigen batches from the same isolate show immunological and biochemical differences

We investigated the occurrence of antigenic and biochemical variability among Paracoccidioides brasiliensis antigen batches prepared according to the same protocol. Initially (experiment 1), we analyzed two antigen lots of two human isolates (Bt1 and Bt2), cultured in two media (PYG: bactopeptone, yeast extract, glucose; MMM: McVeigh and Morton medium) in SDS-PAGE and in two immunological tests (immunodiffusion-ID and footpad swelling test-FPT). Afterwards (experiment 2), we compared the antigenic profile of three antigen hatches from three human isolates (Bt1, Bt2 and Bt3) by two-dimensional immunoelectrophoresis (2 D-IEP) against a reference system for P. brasiliensis antigens. In experiment 1, there were important intra- and inter-strain antigenic differences between batches of the fungal isolates cultured on both media. The block titration of the antigen batches for the immunological tests revealed correlation between protein concentration and biological activity in ID and no correlation in FPT. In experiment 2, the reference system for P brasiliensis showed 26 antigen peaks. There were important differences between batches prepared from the same isolate and between batches from different isolates. Our data suggested the occurrence of instability in the synthesis of antigenic components by a same P. brasiliensis isolate, under controlled incubation conditions.

Mouse extensor digitorum longus and soleus show distinctive ultrastructural changes induced by veratrine

We investigated whether veratrine (5 μl, 10 ng/kg) injected into the mouse extensor digitorum longus (EDL) (fast-twitch) and soleus (SOL) (slow-twitch) muscles provokes distinctive ultrastructural disturbances 15, 30 and 60 min later. The mitochondria in SOL were affected earlier (within 15 min) than in EDL. Swelling of the sarcoplasmic reticulum terminal cisternae was more marked in EDL than in SOL and caused distortion of sarcomeres so that fragmentation of myofilaments was more pronounced in EDL. Hypercontracted sarcomeres were seen mainly in SOL and veratrine caused infoldings of the sarcolemma only in this muscle. In both muscles, the T-tubules remained unaffected and by 60 min after veratrine most of the above alterations had reverted to normal. Pretreatment with tetrodotoxin prevented the alterations induced by veratrine. This suggests that most of the alterations resulted from the enhanced influx of Na+ into muscle fibers. These results emphasize the importance of considering the type of muscle when studying the action of myotoxic agents.

Capacidade de carga real (ccr) da caverna de santana, parque estadual turístico do alto ribeira (Petar) - sp, e indicações para o seu manejo turístico

The tourist use of Santana cave provides a row of environmental damages, compromising partially the pristine conditions. Among the measures adopted to avoid this situation, this issue presents a contribution for the physical limits to tourist visitation and the indications for speleotouristic management. It was used the Cifuentes Carrying Capacity method and some basic principles of Visitors Impact Management Framework - VIM. The results show a Real Carrying Capacity (CCR) about 120 visits daily on the cave. The discussions raise a few hypotheses about the alteration in the method used and the temporal frequency between visitors groups, suggesting a provisional CCR of 117 and 135 visitors daily, respectively, during the week and in the weekends and holidays. The conclusions appoint the necessity of a conceptual revision in the Carrying Capacity methods, mainly about its adaptation for tourist management in caves. In the case studied, it is suggested the immediate beginning of environmental monitoring of the cave, to verify the plausibility from proposed visitation limits and the possible contributions for to mitigate the environmental impacts from speleotourism.

Nodule growth and nitrogen fixation of Calopogonium mucunoides L. show low sensitivity to nitrate

It is well established that nitrate is a potent inhibitor of nodulation and nitrogen fixation in legumes. The objective of this study was to demonstrate the relative insensitivity of these processes to nitrate with Calopogonium mucunoides, a tropical South American perennial legume, native to the cerrado (savannah) region. It was found that nodule number was reduced by about half in the presence of high levels of nitrate (15 mM) but nodule growth (total nodule mass per plant) and nitrogen fixation (acetylene reduction activity and xylem sap ureide levels) were not affected. Other sources of N (ammonium and urea) were also without effect at these concentrations. At even higher concentrations (30 mM), nitrate did promote significant inhibition (ca. 50%) of acetylene reduction activity, but no significant reduction in xylem sap ureides was found. The extraordinary insensitivity of nodulation and N2 fixation of C. mucunoides to nitrate suggests that this species should be useful in studies aimed at elucidating the mechanisms of nitrate inhibition of these processes. © 2010 Springer Science+Business Media B.V.

From widespread to microendemic: Molecular and acoustic analyses show that Ischnocnema guentheri (Amphibia: Brachycephalidae) is endemic to Rio de Janeiro, Brazil

Many species of tropical amphibians are restricted to very small ranges, and this microendemism coupled with ongoing habitat loss and susceptibility to emerging pathogens imperils the long-term persistence of these species. Incomplete taxonomic and distributional knowledge may obscure conservation assessment, particularly in putatively widespread species that are typically considered to be of Least Concern in Red List assessments, but that in fact may constitute complexes of partly microendemic species. Such is the case in the Steindachner's Robber Frog, Ischnocnema guentheri which, together with the recently recognized Ischnocnema henselii, is thought to occupy most of the Brazilian Atlantic Forest. To test whether these taxa may constitute a species complex of range-restricted and thus potentially threatened species, we analyzed 160 samples of I. guentheri and/or I. henselii for two molecular markers, 16S rRNA (16S) and recombination activation gene 1 (RAG1). To verify the monophyly of the complex, closely related species were also included in the 16S analysis. Congruent evidence from the molecular data and from analyses of advertisement calls support the existence of six distinct species within the complex: I. guentheri and I. henselii as well as four candidate new species. The lineages are distributed as a mosaic in the Atlantic Forest and are sympatric at some localities without indication of admixture. Their phylogeographical pattern partially agrees with paleo-models for the Atlantic Forest, but also suggests the existence of micro-refugia in less stable areas. I. guentheri, previously considered to be widespread, was found only in its type locality, a reserve within the urban area of Rio de Janeiro city. Although none of the species studied appears highly threatened with extinction, we recommend their IUCN threat status to be re-evaluated carefully for the next comprehensive update of the Red List of Brazil's amphibians. © 2013 Springer Science+Business Media Dordrecht.

Show da fé e de sentido(s): o universo eclesial como mediação sociocultural

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Paleofauna de vertebrados, com ênfase em répteis e mamíferos, dos depósitos quartenários da região da Serra da Bodoquena, Mato Grosso do Sul, Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reality-show: a tv na era da globalização

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Elapid Snake Venom Analyses Show the Specificity of the Peptide Composition at the Level of Genera Naja and Notechis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

E o homem que murmura contra o ensinamento secreto da Yahad será expulso e não mais voltará (1QS 7:17): a expulsão na comunidade judaica do mar Morto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)